scholarly journals Assessment of the nutritive value of toasted castor seed cake-based diets as a reflect on blood profile of weanling wistar albino rats

2021 ◽  
Vol 44 (3) ◽  
Author(s):  
A. F Agboola
Keyword(s):  
Blood Cell ◽  
Castor Seed ◽  
Albino Rats ◽  
Blood Profile ◽  
Seed Cake ◽  
Soyabean Meal ◽  
Serum Biochemical ◽  
Wistar Albino Rats ◽  
Biochemical Indices ◽  
Dietary Treatments

Since the cost of feed is increasing due to the keen competition between humans and animals, there is a need to critically seek for a matching alternative. Castor seed cake, a residue after the extraction of oil from nutrients-rich castor seed constitutes a waste which can be converted into livestock feed. Thus, the study was carried out to investigate the effect of graded levels of castor seed cake diets on haematological and serum biochemical indices of weanling albino rats in an experiment that lasted 21 days. Thirty weanling Wistar albino rats were weighed individually and randomly assigned to 5 dietary treatments with 3 replicates of two rats in a completely randomized design. Diet 1 was a nitrogen-free diet (NFD), while diets 2, 3, 4, and 5 had 0% (without castor seed cake inclusion), 2, 4 and 6% castor seed cake replacing soyabean meal respectively. At day 21, blood samples were obtained from two rats per replicate for haematological and serum analysis. There were no significant differences (P>0.05) observed in all the parameters (packed cell volume, haemaglobin, red blood cell, white blood cell, lymphocyte, neutrophil, monocyte, eosinophil and platelet) measured. Similarly, diet had no effect on the serum biochemical indices (creatinine, urea, total protein and globulin) measured. Significant difference (P<0.05) was however observed in albumin (2.62 – 3.18g/dL) of rats on the different experimental diets. The albumin concentrations of weanling albino rats on dietary treatments were similar to those on the control diet but significantly (P<0.05) higher (3.09 – 3.18g/dL) than those on the nitrogen free diet. It can be concluded that castor seed cake can replace soyabean meal up to 6% inclusion level without eliciting detrimental effect on blood profile of weanling wistar albino rats.

scholarly journals Effect of enzymes supplementation on growth performance, haematological and serum biochemical responses of grower rabbits fed sorghum based diets

2020 ◽  
Vol 44 (4) ◽  
pp. 279-286
Author(s):  
M. A. Oguntoye ◽  
U. M. Mafindi ◽  
B. A. Ardo ◽  
I. E. Gangum ◽  
D. Amos
Keyword(s):  
Blood Cell ◽  
Total Protein ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Biochemical Responses ◽  
Randomized Design ◽  
Final Weight ◽  
Serum Biochemical ◽  
Biochemical Indices ◽  
Dietary Treatments

A study was conducted to investigate the effect of sorghum based diets supplemented with enzyme on haematological and serum biochemical responses of grower rabbits. A total of twenty four grower rabbits were used for the investigation which lasted eight weeks. Four experimental diets were formulated which included maize based diet (Control), sorghum based diet supplemented with; Protease (D2), Phytase (D3) and PhytasewithProtease(D4).The rabbits were randomly assigned to the four dietary treatments in a completely randomized design. Rabbitsfed diet containing sorghum with phytase (D3)had high significant (p< 0.05) value of 115.10g for final weight. Least statistically similar values of 1136.80g and 1033.60g were obtained for rabbit fed sorghum with protease plus phytase (D4) and control diet, respectively. The weight gain followed similar trend with the final weight. The dietary treatments had no significant influence (p > 0.05) on all haematological parameters determined. A range of 2.90-3.15 ×10 /l was recorded for red blood cell, and 5.710.60×10/l for White blood cell While MCH and MCV random 27.1632.75pg and 88.5589.35fl, respectively. Sorghum based diet supplemented with enzymes showed significant (p< 0.05) on serum biochemical indices. Parameters that were significantly influenced include; Total protein, globulin, urea, creatinine and cholesterol. Rabbits fed sorghum based diet supplemented with phytase (D3) revealed highest (p < 0.05) values for total protein and globulin. Highest (p<0.05) value of 7.05mmol/l,102.25mmol/l and 4.50mmol/l were recorded for urea, creatinine and cholesterol, respectively in the control diet. In conclusion, sorghum based diets supplemented with enzyme improved rabbit performance, had no allergic effect on health status of rabbit and enhanced serum protein bioavailability with efficient nutrient utilization.  

Haematological and serum biochemical indices of growing rabbits fed graded levels of Yam peel meal as replacement for maize

2020 ◽  
Vol 47 (4) ◽  
pp. 167-175
Author(s):  
I. B. Amaza ◽  
A. Maidala ◽  
C. E. Isidahomen
Keyword(s):  
Biochemical Analysis ◽  
Experimental Period ◽  
Blood Chemistry ◽  
Feeding Trial ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Inclusion Level ◽  
Completely Randomized Design ◽  
Biochemical Indices ◽  
Dietary Treatments

Haematological and serum biochemical parameters are indicators of physiological, nutritional and pathological status of animals. Investigation of these parameters provide information for diagnostic purposes. The study was conducted to determine the influence of feeding graded levels of yam peel meal (YPM) as a replacement for maize on haematology; blood chemistry was evaluated in rabbits. Thirty- six (New Zealand white X Chinchilla) were randomly assigned to four dietary treatments in a completely randomized design with nine rabbits per treatment. The rabbits were fed diets containing 0, 12.5, 25 and 37.5% of Yam peel meal (YPM) as a replacement for maize, designated as T1, T2, T3 and T4, respectively. The experimental diets and clean drinking water were supplied ad libitum throughout the experimental period of nine weeks. At the end of the feeding trial, three rabbits per treatment were randomly selected for hematological and serum biochemical analysis. All the haematological and serum biochemical indices except AST were affected (P<0.05) by dietary inclusion of YPM. Although most of the parameters investigated were affected by rabbits fed test ingredient, the values fell within the normal literature reference values for rabbits. It can therefore be concluded that up to 37.5% inclusion level of YPM can be fed to growing rabbits without deleterious effects on health status.

Blood Profile and Selected Organ Histopathology of Balami Sheep Fed Shea Cake (Vitellaria paradoxa) Meal

2021 ◽  
pp. 46-56
Author(s):  
D. O. Okunlola ◽  
M. D. Shittu ◽  
O. O. Olatunji ◽  
A. J. Amuda ◽  
B. O. Olateju ◽  
...  
Keyword(s):  
Blood Cell ◽  
Control Diet ◽  
Average Weight ◽  
Blood Profile ◽  
Significant Difference ◽  
Randomized Experimental Design ◽  
Inclusion Level ◽  
Males And Females ◽  
Dietary Treatments ◽  
Representative Samples

The blood profile and histology of selected tissues (kidney, lungs, intestine, and epididymis) of Balami Sheep fed Shea Cake meal supplement was investigated. A total of thirty-two (32) sheep of the average weight range of 11.75-14.75kg comprising an equal number (16) of males and females were randomly allotted to four dietary treatments in a completely randomized experimental design with eight sheep per treatment for twelve weeks duration. Treatment 1 (T1) was a control diet and had zero inclusion level of Shea cake meal (SCM), while treatments 2,3, and 4 had 10%,15%, and 20%  inclusion of SCM, respectively. At the twelfth week of the experiment, two blood samples of 5ml each were taken from each animal from the jugular vein. The first set was preserved in Ethylene Diamine Tetra Acetic Acid (EDTA) bottle for haematological parameters, while the second samples were preserved without EDTA for serum examination. Representative samples of tissues were randomly selected and slaughtered for gross microscopic examination. There was a significant difference (P<0.05) at various levels of inclusion for parameters investigated. The white blood cell (WBC), Red blood cell (RBC), and Packed cell volume (PCV) recorded the highest values at T3 (15% inclusion level) (13.42 x109/L), 2.92x1012/L, 3.75%, respectively). The serum parameters were significantly(p <0.05)different among the treatments. Total protein ranges from 54.78(mg/dl) (T1) - 60.51(mg/dl) (T2), Cholesterol70.15(mg/dl) (T1) - 78.00(mg/dl) (T4). Iron ranges from 58.15(ug/dl) (T1) - 62.88(ug/dl) (T3).The histopathology of tissues (kidney, lungs, intestine, and epididymis) showed no alteration across the treatments (P= .05) in the gross microscopic examinations. No lesion was observed in the lungs, kidney, epididymis, and intestine. The results showed that shea cake meal posed no health risk to Balami sheep and as such recommended as a good supplement in the diets of the sheep.

Performance and blood indices of West African dwarf goats fed graded levels of malted sorghum sprout with enzyme supplementation

2020 ◽  
Vol 47 (4) ◽  
pp. 210-218
Author(s):  
A. O. Adekanbi ◽  
C. F. I. Onwuka ◽  
A. O. Oni ◽  
K. B. Jinadu ◽  
B. J. Adebayo ◽  
...  
Keyword(s):  
Blood Cells ◽  
West African ◽  
Performance Characteristics ◽  
Daily Weight Gain ◽  
Average Weight ◽  
Blood Profile ◽  
West African Dwarf ◽  
Serum Biochemical ◽  
Enzyme Supplementation ◽  
Dietary Treatments

Malted sorghum sprout (MSP) is an agro-industrial by-products rich in organic nitrogen and amino acids which can be used to enhance ruminant nutrition. A study was conducted to assess the nutritive value of MSP as supplementary on the performance characteristics and blood profile of West African dwarf (WAD) goats. MSP was collected, air-dried and then incorporated at different inclusion levels of 0, 10, 20 and 30% to formulate four dietary treatments T , T , T and T , respectively, with T - T being supplemented with Roxazyme G2 1 2 3 4 2 4 enzyme at 0.5g/kg. Twelve WAD bucks with an average weight of 7±0.5kg were assigned into four dietary treatments with three bucks per treatment in a completely randomized design over a period of 84 days. Data were obtained on performance characteristics, haematological and serum biochemical indices. Performance characteristics showed that average concentrate consumed (229.04g/day) and average daily weight gain (44.44 g/day) of WAD goats were significantly (P?0.05) higher in goats fed 20% MSP with enzyme supplementation. Dietary MSP with enzyme supplementation significantly (P?0.05) influenced the total protein (TP), aspartate amino transferase (AST), alanine amino transferase (ALT), white blood cells (WBC) and red blood cells (RBC) across the treatments. 3 3 TP (7.05g/dL) and WBC (7.88 x 10 /mm ) were significantly (P?0.05) higher in goats fed 20% MSP while globulin values increased significantly (P?0.05) across the dietary treatments (3.94 – 5.05g/dL) and is within the normal levels expected of healthy goats. It is concluded that dietary MSP with enzyme supplementation at 20% improved the performance, hematological and serum biochemical indicess of WAD goats without any adverse effects.

scholarly journals Haematological and serum biochemical indices of broiler chickens fed roselle seed (Hibiscus sabdariffa l.) cake based diet as replacement for soyabean meal

2020 ◽  
Vol 46 (2) ◽  
pp. 158-163
Author(s):  
D. N. Onunkwo ◽  
J. C. Ezike ◽  
W. Amaduruonye ◽  
J. Nathaniel ◽  
G. Daniel-Igwe,
Keyword(s):  
Broiler Chickens ◽  
Control Diet ◽  
Density Lipoprotein ◽  
Dietary Treatment ◽  
Soya Bean ◽  
Broiler Chicks ◽  
Seed Cake ◽  
Blood Biochemical ◽  
Serum Biochemical ◽  
Dietary Treatments

Feeding trial experiment was carried out to evaluate the haematological and serum biochemical parameters of broiler chickens fed graded levels of Roselle Seed Cake (RSC) as replacement of a conventional protein source. Proximate composition was done to determine the nutrient content of RSC and experimental diets. The experiment contained graded levels of RSC replacing soya bean cake (SBC). One hundred and twenty (120) day old Ross broiler chickens were used in a Completely Randomized Design (CRD). The broiler chicks were assigned to five dietary treatments with three replicates each with 8 birds per replicate and 24 birds per treatment. Five isonitrogenous and isocaloric diets were formulated for the broiler straight diet. Diet 1 served asthe control diet while diets 2, 3, 4 and 5 contained 8%, 16%, 24% and 32% RSC respectively. Data were collected and analyzed using analysis of variance and the mean was separated using Duncan multiple range test. The findings from the study showed that there were no significant (P?0.05) differences in all the parameters measured for haematology across dietary treatment means except for the white blood cell. The blood biochemical composition showed no significant (P?0.05) differences in total protein, high density lipoprotein, low density lipoprotein and creatinine across dietary treatments. RSC as an alternative feedstuff can replace soya bean cake at 24% in the diet of broiler chickens for improved haematological and blood biochemical of broilers. Roselle seed cake can replace SBC at the broiler chicken straight ration at 75% (24% dietary RSC) inclusion for better performance.

scholarly journals Tubular Necrosis, Acid-Base and Electrolyte Abnormalities Associated with Gasoline Vapour-induced Nephrotoxicity

2020 ◽  
Vol 2 (3) ◽  
Keyword(s):  
Renal Function ◽  
Biochemical Markers ◽  
Histopathological Examination ◽  
Albino Rats ◽  
Renal Vasculature ◽  
Tubular Necrosis ◽  
Serum Biochemical ◽  
Group 2 ◽  
Wistar Albino Rats ◽  
Electrolyte Abnormalities

Introduction: This study aimed to assess the effect of exposure to gasoline vapor (GV) on the histomorphology and biochemical markers of renal function in rats. Methods: Twenty-four mature Wistar Albino rats weighing 180–200 g were randomly divided into two groups (n = 12 per group). Animals in group 1 (G1) served as unexposed controls, while animals in group 2 (G2) were exposed to GV for 35 days. At the end of the exposure, the animals were sacrificed, and blood samples were collected for biochemical analysis while the kidneys were removed and processed for histopathological evaluation. Results: Serum biochemical markers of renal function in the exposed group differed significantly (p< 0.05) from the unexposed group in urea (45.16 ± 1.00mg/dl versus(vs) 13.20 ± 0.69 mg/dl), creatinine (1.16 ± 0.27mg/dl vs 0.38 ± 0.10mg/dl), uric acid (3.66 ± 0.82mmol/L vs 1.96 ± 0.08mmol/L), potassium (6.90 ± 0.27mmol/L vs 3.57 ± 0.26mmol/L), sodium (182.60 ± 3.21mmol/L vs 141.33 ± 10.46mmol/L), chloride (119.00 ± 1.58mmol/L vs 103.33 ± 2.07mmol/L), pH (6.82 ± 0.22 vs 7.38 ± 0.25), bicarbonate (16.60 ± 5.03mmol/L vs 26.50 ± 3.45mmol/L), and glucose (125.60 ± 16.23mg/ dl vs 83.33 ± 4.46mg/dl). Histopathological examination of kidney sections revealed areas of degenerative and necrotic changes in the glomerulus, tubules, and renal vasculature, particularly in the cortical portion of the kidney. Conclusion: Chronic exposure to gasoline compounds may be associated with significant structural and biochemical derangements in kidney function.

scholarly journals Effect of phytobiotics inclusion on haematological and serum indices of broiler chickens

2015 ◽  
Vol 42 (2) ◽  
pp. 168-173
Author(s):  
O. E. Lawal ◽  
D. Eruvbetine ◽  
R. A. Sobayo ◽  
O. O. Olowofeso
Keyword(s):  
Broiler Chickens ◽  
Broiler Chicken ◽  
Cholesterol Concentration ◽  
Broiler Chicks ◽  
Randomized Design ◽  
Red Blood Cell Count ◽  
Serum Biochemical ◽  
Completely Randomized Design ◽  
Biochemical Indices ◽  
Dietary Treatments

The effect of phytobiotics inclusion on haematological and serum indices of broiler chickens was investigated using 180 one day-old Arbor-acre broiler chicks in an eight weeks feeding trial. Six experimental diets were formulated to include the control diets (Og/kg), Ginger (5g/kg), Pawpaw leaf (5g/kg), Moringa (5g/kg), Turmeric(1g/kg) and Oxytetracycline (0.5g/kg) diet. These diets were assigned to six groups of 30 chicks replicated 3 times with 10 birds per replicate in a completely randomized design (CRD). Feed and water were given ad libitum. At the end of eight weeks, blood samples were collected from jugular vein of the birds and were analyzed for haematological and serum biochemical indices. The result of the analysis showed that dietary treatments significantly (p<0.05) influenced all haematological parameters studied. Ginger had the highest value of packed cell volume (45%), haemoglobin (15.05 g/dl) and red blood cell count (3.39x1012L-1) at the finisher phase. Total protein, uric acid, glucose were significant (p<0.05). Serum cholesterol value was significantly (p<0.05) lower in birds supplemented with ginger compared to other groups. It can be concluded that ginger at 5g/kg improved PCV, Hb and reduction of cholesterol concentration in broiler chicken.

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