scholarly journals Emergence of Dengue Virus Infection in Nepal

2013 ◽  
Vol 1 (3) ◽  
pp. 79-84 ◽  
Author(s):  
Birendra Prasad Gupta ◽  
K. D. Manandhar ◽  
R. Malla ◽  
C. Tamarakar ◽  
S. K. Mishra ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Rural Areas ◽  
Public Health Problem ◽  
Viral Disease ◽  
Infected Mosquito ◽  
Dengue Virus Infection ◽  
Female Ratio ◽  
First Case ◽  
Positive Rate

This article reviews Dengue, a common viral disease in humans and is an emerging public health problem in Tarai Region of Nepal. The most affected are among the poorest populations living in remote, rural areas and urban slums who have even no access for medical treatment, acquired by bite of infected mosquito. Aedes Aegypti infected with dengue virus is the major source of infections for humans and cannot be transmitted from person-to-person because human are the dead end host. DENV-1 was first isolated by Ren Kimura and Susumu Hotta in Japan in 1943. An epidemic of DF involving at least 200,000 cases had occurred between 1942 and 1944 during World War II in Japanese port cities such as Nagasaki, Kobe, and Osaka. First case of dengue was reported in 2004 in Nepal. The seroprevalence study were done in different part of Nepal by IgM antibody capture ELISA and positive rate was highest (50.0%) in Biratnagar, and lowest (19.6%) in Chitwan male to female ratio was 2:1. IgM-positive rate was 29.0% at ages 21-30, 25.4% at ages 11-20 and 23.6% at ages 0-10, but 10.9% at ages 31-40, and ages over 40. There was not significant association between occupation of the patients and positive rate among farmer, labour, service, business and student. The epidemiological studies of Dengue virus infection and the knowledge of the pattern of the disease outbreak can guide therapy and effective preventive measures against this disease.DOI: http://dx.doi.org/10.3126/ijasbt.v1i3.8384 Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 79-84

scholarly journals Seroprevalence 0f Dengue Virus Infection in Nepal

2013 ◽  
Vol 1 (4) ◽  
pp. 224-227 ◽  
Author(s):  
B P Gupta ◽  
S K Mishra ◽  
K D Manandhar ◽  
R Malla ◽  
C S Tamarakar ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Health Problem ◽  
Public Health Problem ◽  
Cross Sectional Study ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Positive Rate ◽  
History Of

Dengue Virus infection is an emerging mosquito-borne disease. It is a global health problem and its expanding endemicity towards new territories is a serious concern. Relatively a new disease in Nepalese context, dengue abruptly appeared as massive outbreak in 2010, merely four years after its first introduction. It is a nagging public health problem in the low lands of Terai, expanding to new areas of Nepal in recent years.  A cross-sectional study was conducted to determine anti-Dengue IgM positive rate in Lumbini, Dhading and Chitwan district. The study was carried from June 2012 to November 2012. The total number of Serum samples was collected from 275 patients visiting hospitals with history of fever, headache and suspected DF. The samples were examined by ELISA. The anti-Dengue IgM positivity was found to be 29.09 %. The positive rate was highest in Dhading (70.37%) followed by Bharatpur (37.6%) and Lumbini (11.38%). The Dengue positive cases were higher in males (32.5 %) than female (24.8 %). The highest positive cases (41.6%) were from age group less than 15 years. Dengue has substantial expansion in Western and Far Western Terai region of Nepal which was limited to the middle Terai region in the past and mostly infects older people.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9135  Int J Appl Sci Biotechnol, Vol. 1(4): 224-227

scholarly journals SERO-EPIDEMIOLOGY OF DENGUE VIRUS INFECTION IN CITIES OF INDONESIA

2013 ◽  
Vol 4 (4) ◽  
pp. 26
Author(s):  
Soegeng Soegijanto ◽  
Kris Cahyo Mulyanto ◽  
Siti Churotin ◽  
Tomohiro Kotaki ◽  
Masa Nori Kamioka ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Rural Areas ◽  
Virus Isolation ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Dengue Virus Infection ◽  
Virus Serotype ◽  
Urban And Rural Areas ◽  
Predominant Strain

Background: Dengue Virus Infektion is major public health problem in Indonesia. Aedesaegypti is widespread in both urban and rural areas, where multiple virus Serotype are circulating. On 2013 outbreak of dengue virus infection occur in East Java. Therefore study seroepidemiology in Bangkalan and Lombok had been done. Aim: to find a mutated strain of Dengue Virus in 4 cities of Indonesia. Method: On 2011 and 2012 seroepidemiology study had been done in Dr. Soetomo Surabaya and Soerya Sidoarjo Hospital; and on  2013 study had been done in Surabaya, Bangkalan and Lombok Hospital . Diagnosis of Dengue Virus Infection was based on Criteri WHO - 2009. Virus isolation in Surabaya, Sidoarjo, Bangkalan and Lombok had been done. Result: a total of 349 isolate were obtained from dengue patients sera collected in Surabaya and Sidoarjo, 2011–2012 showed that Den V1 (182), Den V2 (20) Den V4 (1) were found in Surabaya on 2011 and Den V 1 (79) , Den V 2 (7) were found in Surabaya on 2012; Den V1 (40), Den V 2 (3) were found in Sidoarjo on 2011 and Den V 1 (17) were found in Sidoarjo on 2012; Virus isolation in Surabaya on 2013, January: 237 serum sample were collected, found Den V 1 (8), Den V 3 (2) and Den V 4 (5). And PCR stereotyping of isolated viruses in Madura found Den V 1 (1) and Den V 4 (23). In Lombok found Den V 4 (4).It is possible to shift predominant strain in Surabaya , Genotype or Serotype shift might increase the number of dengue patients. Conclusion: there were shift predominant strain in Surabaya especially Den V 1. Therefore to continuous surveillance of circulating viruses is required to predict the risk of DHF and DF.

Case Report: Transmission of Dengue Virus from a Deceased Donor to a Kidney Transplant Recipient Previously Infected by Dengue Virus

2021 ◽  
Author(s):  
Arnaud Lecadieu ◽  
Laura Teysseyre ◽  
Kevin Larsen ◽  
Charles Vidal ◽  
Margot Caron ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Kidney Transplant ◽  
Kidney Transplant Recipient ◽  
Deceased Donor ◽  
Severe Dengue ◽  
Reunion Island ◽  
Dengue Virus Infection ◽  
First Case ◽  
Réunion Island

Since 2018, a dengue epidemic has been ongoing in the French overseas department of Reunion Island, in the Indian Ocean, with more than 25,000 serologically confirmed cases. Currently, three dengue serotypes have been identified in Réunion Island (DENV-1, DENV-2, and DENV-3) progressing in the form of epidemic outbreaks. This arbovirus is mainly transmitted by mosquitoes of the genus Aedes and may be responsible for serious clinical forms. To date, very few cases of kidney transplant–related dengue virus infection have been described. Here we report the first case of severe dengue virus infection related to kidney transplantation from a patient previously infected with dengue. Testing for dengue fever with PCR search in donor’s urine may help complete the pretransplant assessment in areas where this disease occurs.

scholarly journals Imunocompetent Mice Model for Dengue Virus Infection

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Denise Gonçalves ◽  
Rafael de Queiroz Prado ◽  
Eric Almeida Xavier ◽  
Natália Cristina de Oliveira ◽  
Paulo Marcos da Matta Guedes ◽  
...  
Keyword(s):  
Animal Model ◽  
Virus Infection ◽  
Dengue Virus ◽  
Viral Disease ◽  
Mice Model ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
Dengue Disease ◽  
The Family ◽  
The World

Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the familyFlaviviridae, genusFlavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world. The absence of an appropriate animal model for studying the disease has hindered the understanding of dengue pathogenesis. In our study, we have found that immunocompetent C57BL/6 mice infected intraperitoneally with DENV-1 presented some signs of dengue disease such as thrombocytopenia, spleen hemorrhage, liver damage, and increase in production of IFNγand TNFαcytokines. Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR. Thus, this animal model could be used to study mechanism of dengue virus infection, to test antiviral drugs, as well as to evaluate candidate vaccines.

Neonate with haemophagocytic lymphohistiocytosis secondary to dengue infection: a case report

2016 ◽  
Vol 47 (3) ◽  
pp. 253-255 ◽  
Author(s):  
MV Krithika ◽  
Prakash Amboiram ◽  
Sneha M Latha ◽  
Binu Ninan ◽  
Febe Renjitha Suman ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Case Report ◽  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Haemophagocytic Lymphohistiocytosis ◽  
Dengue Virus Infection ◽  
The Third ◽  
First Case

We report the first case of haemophagocytic lymphohistiocytosis (HLH) in a neonate secondary to primary Dengue virus infection. This neonate presented in the third week of life with fever, shock and hepatosplenomegaly and was diagnosed to have Dengue infection by serology and HLH was confirmed on bone marrow.

Dengue Virus Associated Hemophagocytic Syndrome with Neurogical Manifestations in Children: A Case Report.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4516-4516
Author(s):  
Yadira Soler-Rosario ◽  
Nilka J. Barrios ◽  
Ricardo Garcia ◽  
Alicia Fernandez-Sein ◽  
Enid Rivera
Keyword(s):  
Bone Marrow ◽  
Virus Infection ◽  
Dengue Virus ◽  
Hemophagocytic Syndrome ◽  
Conflicts Of Interest ◽  
Neurological Manifestations ◽  
Positive Serology ◽  
Dengue Virus Infection ◽  
First Case ◽  
Uncommon Presentation

Abstract Abstract 4516 Dengue fever, caused by dengue virus, can cause increased vascular permeability, which leads to a bleeding diathesis or disseminated intravascular coagulation known as Dengue Hemorrhagic Fever (DHF). Hemophagocytocytic Syndrome (HPS) with neurological manifestations is an uncommon presentation of DHF. There are no reports of virus associated with HPS and neurological manifestations in the pediatric literature. Hemophagocytic Syndrome (HPS) is a clinico-pathologic entity characterized by proliferation of T lymphocytes and macrophages leading to cytokine overproduction. HPS may be diagnosed in association with malignant, genetic, or autoimmune diseases. Dengue virus is considered non-neurotropic, however neuroinvansion has been reported. We present the case of a 10 month old female patient who developed DHF, manifested with upper GI bleeding, hypotension and pancytopenia. The infant subsequently developed dengue shock syndrome, with thrombocytopenia, intravascular hemolysis, coagulopathy, elevated transaminase, hyperbilirubinemia, and creatine kinase (CK) of 2,876U/L. She required artificial hemodynamic and blood components support. During her course of illness, she developed ecchymosed, purpuric bullae lesions of the skin in the distal upper extremities. Her IgM ELISA for Dengue virus was positive. Bone marrow aspiration and biopsy was diagnostic for HPS. The patient was initiated on IV steroids and antibiotics therapy. An improvement was noticed on day 8 of illness and was weaned off completely of all artificial support by the day 9. A complete recovery of her hematological, transaminase, billirubin, and coagulation parameters was noticed on day 12. A repeated bone marrow aspirate and biopsy examination was normal. Patient's recovery was significant by muscle weakness (MCG grade ≤3), hypotonia, reduced tendon reflex and increased CK suggestive of possible myositis. The cerebrospinal fluid and magnetic resonance imaging studies of the brain were normal. She was given IV immunoglobulin (500mg/kg) for 2 consecutive days with remarkable neurological improvement including a normal CK (56U/L).She was weaned of steroids completely on day 20.Dengue virus infection was confirmed by a positive serology result at the convalescent stage. On day 23 she had a complete clinical recovery. To our knowledge, this is the first case reported of dengue virus-associated HPS and neurological manifestations in a pediatric patient with DHF. Clinicians should consider that the occurrence of HPS and neurological manifestations in children could de due to dengue virus infection. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Sero-diagnosis of Dengue virus in Different Hospitals of Nepal

2013 ◽  
Vol 1 (2) ◽  
pp. 58-62 ◽  
Author(s):  
Y Shah ◽  
G Khadka ◽  
GP Gupta ◽  
N Adhikari ◽  
A Poudel ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Control Measure ◽  
Public Health Problem ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dengue Virus Infection ◽  
Capture Elisa ◽  
Occupation Group ◽  
Hilly Region

INTRODUCTION: Dengue fever (DF) is an emerging mosquito borne viral disease and important public health problem in low land Terai region which is also moving towards hilly region Nepal. This study was designed to determine the sero-prevalence of dengue virus infection in patients visiting hospitals of Nepal. MATERIALS AND METHODS: This study was conducted during period (June-November) of 2010 in Nepalese patients with fever visiting hospitals of Birganj, Damouli, Biratanagar, Dhading Besi and Chitwan. The sero-prevalence of dengue virus specific IgM was determined by enzyme linked immunosorbent assay (ELISA). Serum samples were collected from 289 patients visiting hospitals with history of fever and clinically suspected dengue fever. RESULTS: The anti-dengue IgM positivity was found to be 8.99%. The positive dengue cases were higher in male (10.8%) as compared to female (7.1%) though it was not statistically significant (P>0.05). Among different age groups, the highest positive cases (12.3%) were from age group below 15 years followed by above 50 years 8.3%. Out of 5 hospitals, the highest positive cases were in Tanahu hospital, Damouli (23.8%) followed by Bharatpur hospital and Chitwan (22.2%). Age and gender were found to be independent predictors. The highest numbers of dengue positive cases were in occupation group business (13.3%) followed by agriculture (12.7%). CONCLUSIONS: Prevalence of dengue virus infection is increasing and proper control measure should be provided. IgM capture ELISA was used for laboratory analysis and remains as a reliable and inexpensive method for the diagnosis of dengue. Hence, the IgM capture ELISA has become the most accepted technique for the diagnosis of dengue in developing countries like Nepal. DOI: http://dx.doi.org/10.3126/ijim.v1i2.7003 Int J Infect Microbiol 2012;1(1):58-62

scholarly journals GAMBARAN NILAI HEMATOKRIT DAN LAJU ENDAP DARAH PADA ANAK DENGAN INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Sitti S. P. Kamuh ◽  
Arthur E. Mongan ◽  
Maya F. Memah
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Clinical Symptoms ◽  
Clinical Manifestations ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Dengue Virus Infection ◽  
Erythrocyte Sedimentation

Abstract: Dengue is a major public health problem that can be found throughout the tropical and subtropical countries around the world. Spectrum of clinical manifestations of dengue virus infection varies greatly. In addition to clinical symptoms, the diagnosis of dengue virus infection need to be supported by blood tests such as hematocrit and erythrocyte sedimentation rate. This study aimed to determine the hematocrit value and erythrocyte sedimentation rate (ESR) in children with dengue virus infection in Manado. This study used a cross-sectional design and was conducted from Desember 2014 to January 2015 at GMIM Pancaran Kasih, Advent, and Robert Wolter Mongisidi hospitals in Manado. There were 37 patients that fulfilled the inclusion criteria, consisted of 17 males and 20 females. The results showed that of 37 patients, there were 36 with hematocrit within normal limits. Moreover, of 37 patients only 6 patients performed the ESR examination; only 1 child had a rapid ESR result (> 15mm/h). Conclusion: In this study, most of the pediatric patients with dengue virus infection in Manado had normal hematocrit. Of 6 patients who had performed ESR test, only 1 had rapid ESR.Keywords: dengue, hematocrit, erythrocyte sedimentation rateAbstrak: Dengue merupakan masalah kesehatan masyarakat yang utama dan dapat ditemui diseluruh daerah tropis dan subtropis di seluruh dunia. Spektrum manifestasi klinis infeksi virus dengue sangat bervariasi. Selain gejala klinis, diagnosis infeksi virus dengue perlu ditunjang hasil uji darah di laboratorium antara lain hematokrit dan laju endap darah (LED). Penelitian ini bertujuan untuk mengetahui nilai hematokrit dan LED pada anak dengan infeksi virus dengue di Manado. Penelitian ini menggunakan desain potong lintang, dilakukan pada bulan Desember 2014 sampai Januari 2015 di RSU GMIM Pancaran Kasih Manado, RS Advent Manado, dan RSAD Robert Wolter Mongisidi Teling17 anak laki-laki dan 20 anak perempuan. Hasil penelitian memperlihatkan 36 dari 37 pasien mempunyai hematokrit normal. Dasri 37 pasien, hanya 6 anak yang menjalani pemeriksaan LED; hanya 1 anak dengan LED cepat (> 15mm/jam). Simpulan: Pada studi ini, sebagian besar pasien anak dengan infeksi virus dengue di Manado mempunyai nilai hematokrit normal. Dari 6 pasien yang dilakukan pemeriksaan LED, 1 anak mempunyai hasil LED cepat.Kata kunci: dengue, hematrokrit, laju endap darah.

scholarly journals Upaya Pencegahan dan Pengendalian Demam Berdarah Dengue dalam Bentuk Peta Tematik di Kecamatan Rappocini

bionature ◽  
2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Suci Wulandhani ◽  
A. Bida Purnamasari ◽  
Ryan Humardani Syam Pratomo
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Hemorrhagic Fever ◽  
Viral Disease ◽  
High Rate ◽  
Dengue Virus Infection ◽  
Dengue Disease ◽  
Keywords Prevention ◽  
Short Time ◽  
And Control

Abstract. Dengue hemorrhagic fever (DHF) is a disease caused by dengue virus infection. One symptom of dengue virus infection is high fever and headache. Dengue virus is a virus from the genus Flavivirus, family of Flaviviridae. This dengue fever is a dangerous viral disease because it can cause sufferers to die in a very short time / several days. The existence and population density are often associated with transmission, endemicity and Extraordinary Events (EE) of DHF. Disease mapping by utilizing digital technology to support epidemiological investigations and also as a tool to monitor regional conditions for dengue disease. The purpose of this study was to determine efforts to prevent and control dengue disease by mapping. The results of the study show that mapping can be seen as increasing and decreasing the number of cases of dengue disease so that it can assist in data management and reporting of information to monitor areas at risk of contracting dengue disease. Suggestions given need to do further research using other variables that are the cause of the high rate of dengue cases, so that it can be utilized by related agencies as the basis of the information system supporting the decision on preventive measures to combat dengue. Keywords: prevention and control, DHF, mapping.

scholarly journals HITUNG JENIS LEUKOSIT PADA PASIEN ANAK DENGAN INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Ellisabeth M. Harahap ◽  
Arthur E. Mongan ◽  
Maya F. Memah
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Viral Infection ◽  
Viral Disease ◽  
Differential Count ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
The Third ◽  
The World ◽  
Cross Sectional Design

Abstract: Dengue is the most rapidly spreading mosquito-borne viral disease in the world. The decreasing leukocytes can be found between the third and eighth day with normal differential telling. The number of granulocytes decreases on the third until the eighth day. This study used a cross-sectional design. esearch. Samples were children with dengue virus infection at Wolter Mongisidi Hospital, Advent Hospital, and Pancaran Kasih Hospital in Manado from December 2014 until January 2015. There were 36 children as samples. The results showed that 48.6% of samples had increases of basophils, 54% had decreases of eosinophils, 64.8% had decreases of neutrophils, 54% had increases of lymphocytes, and 59.4% had increases of monocytes. Conclusion: In this study, most of the children with dengue viral infection had decreased number of neutrophil.Keywords: dengue viral infection, children, differential count, leukocyteAbstrak: Infeksi virus dengue adalah penyakit virus ditularkan oleh nyamuk dengan penyebaran paling cepat di dunia. Penurunan leukosit dapat dijumpai antara hari ke 1-3 demam dengan hitung jenis yang masih dalam batas normal. Jumlah granulosit menurun pada hari ke 3-8. Penelitian ini bertujuan untuk mengetahui hitung jenis leukosit pada anak terinfeksi virus dengue di Manado. bersifat potong lintang. Penelitian ini menggunakan desain potong lintang. Sampel penelitian ialah pasien anak yang terinfeksi virus dengue di RS Wolter Mongisidi Manado, RS Advent Manado, dan RS Pancaran Kasih Manado selama bulan Desember 2014 sampai Januari 2015. Jumlah sampel sebanyak 36 anak. Hasil penelitian menunjukkan 48,6% sampel mengalami peningkatan jumlah basofil, 54% penurunan eosinofil, 64,8% penurunan neutrofil, 54% peningkatan limfosit, dan 59,4% peningkatan monosit. Simpulan: Pada penelitian ini sebagian besar pasien anak terinfeksi virus dengue menunjukkan penurunan neutrofil.Kata kunci: infeksi virus dengue, anak, hitung jenis, leukosit

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