scholarly journals Imunocompetent Mice Model for Dengue Virus Infection

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Denise Gonçalves ◽  
Rafael de Queiroz Prado ◽  
Eric Almeida Xavier ◽  
Natália Cristina de Oliveira ◽  
Paulo Marcos da Matta Guedes ◽  
...  
Keyword(s):  
Animal Model ◽  
Virus Infection ◽  
Dengue Virus ◽  
Viral Disease ◽  
Mice Model ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
Dengue Disease ◽  
The Family ◽  
The World

Dengue fever is a noncontagious infectious disease caused by dengue virus (DENV). DENV belongs to the familyFlaviviridae, genusFlavivirus, and is classified into four antigenically distinct serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. The number of nations and people affected has increased steadily and today is considered the most widely spread arbovirus (arthropod-borne viral disease) in the world. The absence of an appropriate animal model for studying the disease has hindered the understanding of dengue pathogenesis. In our study, we have found that immunocompetent C57BL/6 mice infected intraperitoneally with DENV-1 presented some signs of dengue disease such as thrombocytopenia, spleen hemorrhage, liver damage, and increase in production of IFNγand TNFαcytokines. Moreover, the animals became viremic and the virus was detected in several organs by real-time RT-PCR. Thus, this animal model could be used to study mechanism of dengue virus infection, to test antiviral drugs, as well as to evaluate candidate vaccines.

scholarly journals Upaya Pencegahan dan Pengendalian Demam Berdarah Dengue dalam Bentuk Peta Tematik di Kecamatan Rappocini

bionature ◽  
2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Suci Wulandhani ◽  
A. Bida Purnamasari ◽  
Ryan Humardani Syam Pratomo
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Hemorrhagic Fever ◽  
Viral Disease ◽  
High Rate ◽  
Dengue Virus Infection ◽  
Dengue Disease ◽  
Keywords Prevention ◽  
Short Time ◽  
And Control

Abstract. Dengue hemorrhagic fever (DHF) is a disease caused by dengue virus infection. One symptom of dengue virus infection is high fever and headache. Dengue virus is a virus from the genus Flavivirus, family of Flaviviridae. This dengue fever is a dangerous viral disease because it can cause sufferers to die in a very short time / several days. The existence and population density are often associated with transmission, endemicity and Extraordinary Events (EE) of DHF. Disease mapping by utilizing digital technology to support epidemiological investigations and also as a tool to monitor regional conditions for dengue disease. The purpose of this study was to determine efforts to prevent and control dengue disease by mapping. The results of the study show that mapping can be seen as increasing and decreasing the number of cases of dengue disease so that it can assist in data management and reporting of information to monitor areas at risk of contracting dengue disease. Suggestions given need to do further research using other variables that are the cause of the high rate of dengue cases, so that it can be utilized by related agencies as the basis of the information system supporting the decision on preventive measures to combat dengue. Keywords: prevention and control, DHF, mapping.

scholarly journals HITUNG JENIS LEUKOSIT PADA PASIEN ANAK DENGAN INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Ellisabeth M. Harahap ◽  
Arthur E. Mongan ◽  
Maya F. Memah
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Viral Infection ◽  
Viral Disease ◽  
Differential Count ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
The Third ◽  
The World ◽  
Cross Sectional Design

Abstract: Dengue is the most rapidly spreading mosquito-borne viral disease in the world. The decreasing leukocytes can be found between the third and eighth day with normal differential telling. The number of granulocytes decreases on the third until the eighth day. This study used a cross-sectional design. esearch. Samples were children with dengue virus infection at Wolter Mongisidi Hospital, Advent Hospital, and Pancaran Kasih Hospital in Manado from December 2014 until January 2015. There were 36 children as samples. The results showed that 48.6% of samples had increases of basophils, 54% had decreases of eosinophils, 64.8% had decreases of neutrophils, 54% had increases of lymphocytes, and 59.4% had increases of monocytes. Conclusion: In this study, most of the children with dengue viral infection had decreased number of neutrophil.Keywords: dengue viral infection, children, differential count, leukocyteAbstrak: Infeksi virus dengue adalah penyakit virus ditularkan oleh nyamuk dengan penyebaran paling cepat di dunia. Penurunan leukosit dapat dijumpai antara hari ke 1-3 demam dengan hitung jenis yang masih dalam batas normal. Jumlah granulosit menurun pada hari ke 3-8. Penelitian ini bertujuan untuk mengetahui hitung jenis leukosit pada anak terinfeksi virus dengue di Manado. bersifat potong lintang. Penelitian ini menggunakan desain potong lintang. Sampel penelitian ialah pasien anak yang terinfeksi virus dengue di RS Wolter Mongisidi Manado, RS Advent Manado, dan RS Pancaran Kasih Manado selama bulan Desember 2014 sampai Januari 2015. Jumlah sampel sebanyak 36 anak. Hasil penelitian menunjukkan 48,6% sampel mengalami peningkatan jumlah basofil, 54% penurunan eosinofil, 64,8% penurunan neutrofil, 54% peningkatan limfosit, dan 59,4% peningkatan monosit. Simpulan: Pada penelitian ini sebagian besar pasien anak terinfeksi virus dengue menunjukkan penurunan neutrofil.Kata kunci: infeksi virus dengue, anak, hitung jenis, leukosit

scholarly journals THE MONTHLY CHANGING OF THE LOWEST POPULATION DENGUE VIRUS INFECTION IN PATIENT AT SOERYA HOSPITAL SIDOARJO IN 00

2015 ◽  
Vol 2 (1) ◽  
pp. 20
Author(s):  
Soegeng Soegijanto
Keyword(s):  
Global Warming ◽  
Virus Infection ◽  
Dengue Virus ◽  
Urban Area ◽  
Dengue Shock Syndrome ◽  
Dengue Virus Infection ◽  
The World ◽  
Important Health ◽  
Good Environment ◽  
Growing Population

Background: Dengue virus infection is one of the important health problems in Indonesia, although the mortality rate has been decreased but many dengue shock syndrome cases is very difficult to be solving handled. To solve this problem, some factor that influence the prevalence of dengue virus infection should be studied. The Aim of Study: To detect some factor that maintain the higher case of dengue virus infection in patient at the Soerya Hospital Sepanjang, Sidoarjo. Material & Method: Study had been done at Soerya Hospital Sepanjang, Sidoarjo since January 1, 2007 until December 31, 2010. All cases suspected dengue virus infection in patient at soerya hospital were diagnosed based on WHO criteria in 1997 and PCR examination in ITD laboratory. The Result: In 2007, 2008, 2009, the monthly observation showed that decreasing cases of dengue virus infection in patient at Soerya Hospital had been found on September, but in 2010 this event had been found on November. Why this event to be change? It is suggested might be due to global warming in the world and the climate going to influence the environment sanitation. Interaction between agent host and environment becoming increase it might be due to the changing of climate can influence the growing population Aedes Aegyptie and Aedes Albopictus promoting to increase vector for transmit dengue virus infection. It is prominent in sub urban area, with have many peoples don’t aware with the bad environment sanitation. And many peoples showed very dynamic for living until the idea good environment do not be thought. By this condition the monthly population dengue virus infection in patient at hospital are going to maintain higher more than six months than usually. On the year 2007, 2008, 2009 the lowest cases found on September. In 2010, the lowest cases had been found on November. The Conclusion: Global warming, increasing sub urban area which have many peoples don’t aware with the bad environment sanitation and have highly dynamic peoples for getting some money for their life, could influence the higher cases dengue virus infection in patient at hospital more than 6 months.

scholarly journals Roles for Endothelial Cells in Dengue Virus Infection

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Nadine A. Dalrymple ◽  
Erich R. Mackow
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Capillary Permeability ◽  
Virus Disease ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Barrier Functions ◽  
Dengue Disease ◽  
Primary Fluid

Dengue viruses cause two severe diseases that alter vascular fluid barrier functions, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The endothelium is the primary fluid barrier of the vasculature and ultimately the effects of dengue virus infection that cause capillary leakage impact endothelial cell (EC) barrier functions. The ability of dengue virus to infect the endothelium provides a direct means for dengue to alter capillary permeability, permit virus replication, and induce responses that recruit immune cells to the endothelium. Recent studies focused on dengue virus infection of primary ECs have demonstrated that ECs are efficiently infected, rapidly produce viral progeny, and elicit immune enhancing cytokine responses that may contribute to pathogenesis. Furthermore, infected ECs have also been implicated in enhancing viremia and immunopathogenesis within murine dengue disease models. Thus dengue-infected ECs have the potential to directly contribute to immune enhancement, capillary permeability, viremia, and immune targeting of the endothelium. These effects implicate responses of the infected endothelium in dengue pathogenesis and rationalize therapeutic targeting of the endothelium and EC responses as a means of reducing the severity of dengue virus disease.

scholarly journals NS-1 antigen positive Dengue Infection and molecular characterization of Dengue Viruses in a private Medical College Hospitalin Dhaka, Bangladesh

2018 ◽  
Vol 17 (4) ◽  
pp. 669-673
Author(s):  
Mahmuda Siddiqua ◽  
Ahmed Nawsher Alam ◽  
AKM Muraduzzaman ◽  
Tahmina Shirin
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Medical Science ◽  
Cost Effective ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
Medical College ◽  
Virus Serotype ◽  
Ns1 Antigen

Introduction: Detection of dengue virus infection as soon as possible is critical for management of dengue virus infected patients. Immuno-chromatographic (ICT) tests are easy, cost effective method for dengue virus antigen detection.The sensitivity and specificity of ICT should compare with a gold standard test like RT-PCR. Aim of this study was to compare two test methods (ICT and RT-PCR), observe dengue serotype and seasonal impact on dengue infection.Methodology & result: The patients of Ibn Sina Medical College Hospital from October 2015 to October 2017 were tested for dengue NS1 antigen by ICT method. Out of 3201 sample tested 32.39% were found positive and 89 of which were re-tested for RT-PCR for comparison. Eighty eight of 89 NS1 positive cases showed positive by RT-PCR method giving an accuracy of 98.87%. Among the RT-PCR positive cases 45 were further analyzed for serotype. DEN-1, DEN-2 or both DEN- 1 and DEN-2 were found in 21, 23 and 1cases respectively. No cases of DEN-3 or DEN-4 were detected.Conclusion: This study showed that easily available and cost effective dengue NS1 antigen detection method (ICT) is as effective as molecular test (RT-PCR). DEN-1 and DEN-2 serotype were prevalent during last few years in Bangladesh. Continuous monitoring of dengue virus serotype is important for prevention and control of sudden epidemic by other serotype. Alert to be more during post monsoon when the peak of dengue virus infection was observed.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.669-673

First Report of Dengue Virus Infection in a Bone Marrow Transplant Patient.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5331-5331
Author(s):  
Myrna S. Figueroa ◽  
Luis A. Clavell
Keyword(s):  
Bone Marrow ◽  
Virus Infection ◽  
Dengue Virus ◽  
Bone Marrow Transplant ◽  
Virus Type ◽  
Transplant Patient ◽  
Marrow Transplant ◽  
Allogeneic Bone ◽  
Dengue Virus Infection ◽  
The World

Abstract Dengue virus infections are a frequent cause of morbidity and mortality in certain endemic areas of the world but it has never been reported to occur in a bone marrow transplant patient. We describe a pediatric patient with ALL on her first bone marrow relapse undergoing allogeneic bone marrow transplant who developed sepsis and irreversible shock that on postmortem autopsy proved to be secondary to dengue virus infection Type 4. A 6 years old female with ALL underwent an allogeneic bone marrow transplant from her sibling (6/6 match) on Nov 10, 1994. She received TBI (1200 rads) and ARA-C (3gm/m2 per dose per 12 doses) as preparative regimen. She developed generalized skin erythema on day +5 followed by fever on day +6. The following days were characterized by continued fever spikes, severe mucositis, persistence of the erythematous skin rash (first degree burn like) and gross hematuria in spite of broad spectrum antibiotic coverage. Blood cultures were reported negative. She developed irreversible shock and finally died on day +11 post-transplant. At autopsy (Nov 22, 1994), Dengue virus type 4 was isolated from blood, ascitic fluid, liver and spleen by routine viral culture at San Juan C.D.C. Dengue Branch. Results were confirmed by polymerase chain reaction. Her donor also developed low grade fever the day after bone marrow donation. Further blood testing on donor (Dec 2, 1994) showed positive IgM antibodies against Dengue virus Type 4. The recipient viral cultures matches with donor acute titers for Dengue Type 4, suggesting infection through bone marrow transplant. Dengue virus infection may cause significant morbidity and mortality to blood and bone marrow recipients in endemic areas of the world.

scholarly journals Nine year trends of dengue virus infection in Mumbai, Western India

2017 ◽  
Vol 9 (04) ◽  
pp. 296-302 ◽  
Author(s):  
Jayanthi Shastri ◽  
Manita Williamson ◽  
Nilima Vaidya ◽  
Sachee Agrawal ◽  
Om Shrivastav
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Monsoon Season ◽  
Western India ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
Virus Serotype ◽  
Wide Range ◽  
Ns1 Antigen

Abstract INTRODUCTION: Dengue virus (DENV) causes a wide range of diseases in humans, from acute febrile illness Dengue fever (DF) to life-threatening Dengue hemorrhagic fever (DHF) or Dengue shock syndrome (DSS). Factors believed to be responsible for spread of Dengue virus infection include explosive population growth, unplanned urban overpopulation with inadequate public health systems, poor standing water and vector control, climate changes and increased international recreational, business, military travel to endemic areas. All of these factors must be addressed to control the spread of Dengue and other mosquito-borne infections. The detection of Dengue virus RNA by reverse transcriptase PCR (RT-PCR) in human serum or plasma samples is highly indicative of acute Dengue fever. Moreover, the method is able to identify the Dengue virus serotype by demonstrating defined sequence homologies in the viral genomic RNA. METHODS AND RESULTS: During the nine year period of this study analysis, 6767 strongly suspected cases were tested by RT-PCR. 1685 (24.9%) were Dengue PCR positive and confirmed as Dengue cases. Observations on the seasonality were based on the nine year's data as the intensity of sampling was at its maximum during monsoon season. Dengue typing was done on 100 positive samples after storage of Dengue RNA at – 80°C. Dengue serotypes were detected in 69 samples of which Dengue 2 was most predominant. 576 samples were processed for NS1 antigen and PCR simultaneously. 19/576 were positive (3.3 %) for NS1 as well as by PCR. 23/576 samples were negative for NS1 antigen, but were positive by RT-PCR. The remaining 534 samples which were negative for NS1 antigen were also negative by Dengue RT-PCR. CONCLUSION: In this study we sought to standardize rapid, sensitive, and specific fluorogenic probe-based RT-PCR assay to screen and serotype a representative range of Dengue viruses that are found in and around Mumbai. Qualitative Dengue virus TaqMan assays could have tremendous utility for the epidemiological investigation of Dengue illness and especially for the study of the viremic response with candidate live-attenuated dengue virus vaccines.

scholarly journals Emergence of Dengue Virus Infection in Nepal

2013 ◽  
Vol 1 (3) ◽  
pp. 79-84 ◽  
Author(s):  
Birendra Prasad Gupta ◽  
K. D. Manandhar ◽  
R. Malla ◽  
C. Tamarakar ◽  
S. K. Mishra ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Rural Areas ◽  
Public Health Problem ◽  
Viral Disease ◽  
Infected Mosquito ◽  
Dengue Virus Infection ◽  
Female Ratio ◽  
First Case ◽  
Positive Rate

This article reviews Dengue, a common viral disease in humans and is an emerging public health problem in Tarai Region of Nepal. The most affected are among the poorest populations living in remote, rural areas and urban slums who have even no access for medical treatment, acquired by bite of infected mosquito. Aedes Aegypti infected with dengue virus is the major source of infections for humans and cannot be transmitted from person-to-person because human are the dead end host. DENV-1 was first isolated by Ren Kimura and Susumu Hotta in Japan in 1943. An epidemic of DF involving at least 200,000 cases had occurred between 1942 and 1944 during World War II in Japanese port cities such as Nagasaki, Kobe, and Osaka. First case of dengue was reported in 2004 in Nepal. The seroprevalence study were done in different part of Nepal by IgM antibody capture ELISA and positive rate was highest (50.0%) in Biratnagar, and lowest (19.6%) in Chitwan male to female ratio was 2:1. IgM-positive rate was 29.0% at ages 21-30, 25.4% at ages 11-20 and 23.6% at ages 0-10, but 10.9% at ages 31-40, and ages over 40. There was not significant association between occupation of the patients and positive rate among farmer, labour, service, business and student. The epidemiological studies of Dengue virus infection and the knowledge of the pattern of the disease outbreak can guide therapy and effective preventive measures against this disease.DOI: http://dx.doi.org/10.3126/ijasbt.v1i3.8384 Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 79-84

A simple one-step real-time RT-PCR for diagnosis of dengue virus infection

2008 ◽  
Vol 80 (8) ◽  
pp. 1426-1433 ◽  
Author(s):  
Harryson Wings Godoy dos Santos ◽  
Telma Regina Ramos Silva Poloni ◽  
Kelly Paula Souza ◽  
Vanessa Danielle Menjon Muller ◽  
Flávia Tremeschin ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Real Time ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
One Step

scholarly journals Dengue virus–induced hemorrhage in a nonhuman primate model

Blood ◽  
2010 ◽  
Vol 115 (9) ◽  
pp. 1823-1834 ◽  
Author(s):  
Nattawat Onlamoon ◽  
Sansanee Noisakran ◽  
Hui-Mien Hsiao ◽  
Alexander Duncan ◽  
Francois Villinger ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Rhesus Macaques ◽  
Blood Chemistry ◽  
High Dose ◽  
Dengue Virus Infection ◽  
Primate Model ◽  
Dengue Disease ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

AbstractLack of a dengue hemorrhagic animal model recapitulating human dengue virus infection has been a significant impediment in advancing our understanding of the early events involved in the pathogenesis of dengue disease. In efforts to address this issue, a group of rhesus macaques were intravenously infected with dengue virus serotype 2 (strain 16 681) at 1 × 107 PFU/animal. A classic dengue hemorrhage developed 3 to 5 days after infection in 6 of 6 animals. Blood chemistry appeared to be normal with exception of creatine phosphokinase, which peaked at 7 days after infection. A modest thrombocytopenia and noticeable neutropenia concomitant with slight decrease of hemoglobin and hematocrit were registered. In addition, the concentration of D-dimer was elevated significantly. Viremia peaked at 3 to 5 days after infection followed by an inverse relationship between T and B lymphocytes and a bimodal pattern for platelet-monocytes and platelet-neutrophil aggregates. Dengue virus containing platelets engulfed by monocytes was noted at 8 or 9 days after infection. Thus, rhesus macaques inoculated intravenously with a high dose of dengue virus produced dengue hemorrhage, which may provide a unique platform to define the early events in dengue virus infection and help identify which blood components contribute to the pathogenesis of dengue disease.

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