scholarly journals Physicochemical Analysis and Microbial Diversity of Yamuna Water and Industrial Effluents

2014 ◽  
Vol 2 (2) ◽  
pp. 199-205 ◽  
Author(s):  
Poonam Gupta ◽  
Monika Asthana ◽  
Avnish Kumar ◽  
Siddhartha Barun
Keyword(s):  
Ph Value ◽  
Environmental Concern ◽  
Bacterial Species ◽  
Industrial Effluents ◽  
Chloride Content ◽  
Physicochemical Analysis ◽  
Tannery Effluent ◽  
Tannery Effluents ◽  
Textile Effluents ◽  
Standard Values

Pollution has arisen as a serious environmental concern to the present world after industrialization of human societies. It has severely affected our air, soil and water sources. Looking to its global, national, regional and local dimensions, it is now imperative to check it at each and every level. In the present study, 8 samples (3 Yamuna water samples, 3 tannery effluent samples and 2 textile effluent samples), were collected from different sites of Yamuna and exit points of textile and tannery Industries. Water and effluent samples were analysed for various physicochemical parameters (pH, TDS, hardness, chloride and BOD) using conventional methods. Afterwards these samples were utilized for isolation of the native bacterial species. All the samples were showing higher than the standard values for TDS (500mg/l), hardness (80-100 mg/l), chloride (250mg/l) and BOD (30mg/l). It was observed that the tannery effluents were showing maximum TDS values(1190-1240mg/l), followed by textile effluents (1190 and 1210mg/l) and Yamuna water (530-1180mg/l).Similarly, in case of chloride content, highest concentration range(828.8-1598mg/l)was shown by tannery effluents.  pH value was nearly neutral for Yamuna water, slightly  acidic in case of textile effluents and more acidic for tannery samples. Highest range of hardness values were observed for the tannery effluents (860-880mg/l) followed by textile effluents (760 and 860mg/l). The BOD values were nearly similar for all the samples with maximum values being observed for tannery effluents (42-48 mg/l). Thus it can be inferred that all the samples were highly polluted and need to be treated by suitable methods. There were 11 cultures purified, that could be employed in bioremediation purposes.DOI: http://dx.doi.org/10.3126/ijasbt.v2i2.10352Int J Appl Sci Biotechnol, Vol. 2(2): 199-205 

scholarly journals Study on the comet assay and micronucleus test in eudrilus eugeniae earthworms’ coelomocytes exposed with tannery industrial effluents

2020 ◽  
Vol 7 (2) ◽  
pp. 63-67
Author(s):  
Mathivanan R ◽  
Chandirasekar R
Keyword(s):  
Comet Assay ◽  
Experimental Animal ◽  
Micronucleus Test ◽  
Terrestrial Ecosystem ◽  
Industrial Effluents ◽  
Tannery Effluent ◽  
Eudrilus Eugeniae ◽  
High Concentration ◽  
Tannery Effluents ◽  
Test Result

Tannery effluents having the different kind’s chemical compounds are extensively used to the production of leather industries and it’s considered as rich potential of environmental pollutant.Earthworms are easily affecting the toxic chemical in environmentally and in this organism is good experimental animal for monitoring the soil pollution and terrestrial ecosystem. In this study, we taken from the industrial raw tannery effluents and the experimental animal in earthworm species of Eudrilus eugeniae (10 for each group) were introduced to 48 hrs for tannery effluent in five different concentrations like 10 ml, 20ml, 30ml, 40 ml, and 50 ml and to find out LC50 level. The LC50 was found at 35 ml concentration. The study was used to the LC50/2, LC50, 2XLC50 for 48 hrs. Then the genotoxicity level was evaluated in Eudrilus eugeniae species. After that we collect earthworm’s coelomocytes by using the micronucleus (MN) test and comet assay (CA) test. Result of the study, MN and CA level was significantly increased in both genotoxicity and cytotoxicity assays and the high concentration of effluent to promote the increased level of DNA damage and micronucleus in Eudrilus eugenie species.

scholarly journals Biodegradation of Tannic Acid, Chromium and Cadmium Present in Leather Industrial Effluents Using Microorganisms Isolated from Leather Industrial Sludge

2021 ◽  
Vol 8 (12) ◽  
pp. 503-512
Author(s):  
Roselin K ◽  
J. Caroline Rose
Keyword(s):  
Tannic Acid ◽  
Industrial Effluents ◽  
Tannery Effluent ◽  
Tannery Sludge ◽  
Bacterial Strains ◽  
Bacillus Velezensis ◽  
Tannery Effluents ◽  
Industrial Sludge ◽  
Tannery Waste ◽  
Paracoccus Pantotrophus

The present work was aimed to isolate indigenous predominant adapted Bacterial strains from tannery waste which possess the ability to detoxify and degrade Tannic acid, Chromium and Cadmium from tannery effluent. Fifteen bacterial strains were isolated from tannery sludge samples out of which Paracoccus pantotrophus (Tannery Waste 15) and Bacillus velezensis (Tannery Waste 17) were found to be the most efficient isolates. Degradation of Tannic acid, Cadmium and Chromium were evaluated for the two selected isolates. Better degradation of heavy metals was recorded in co-cultured media on day 7. From the study, it is evident that both P. pantotrophus and B. velezensis have has the ability to degrade tannic acid with maximum degradation on day 7 and absorbance was found to be 0.915 and 0.383 respectively. The strain P. pantotrophus showed better tannic acid degradation than B. velezensis. Better degradation was observed with co-culturing of both the strains with absorbance of 0.274. Optimal cadmium degradation was observed on day 7 with OD 2.013 and 1.709 for B. velezensis and P. pantotrophus respectively. P. pantotrophus showed better cadmium degradation when compared to B. velezensis. Chromium degradation was maximum on day 7 and absorbance was 2.096 for P. pantotrophus and 0.560 for B. velezensis. The isolates recorded an acceptable reduction in the concentration of Tannin, Chromium and Cadmium in tannery effluent. The results of this showed that the isolates reduced the concentration of Tannin, Chromium and Cadmium present in the raw tannery effluent and suggest that the organisms can be used as a possible treatment of tannery effluents. Keywords: Bio-degradation, Bacillus velezensis, Paracoccuspantotrophus, Chromium, Cadmium, Tannic acid, Tannery effluent.

scholarly journals Biodegradation of tannery effluent and its impact on seed germination of Oryza sativa

2020 ◽  
Vol 10 (3) ◽  
pp. 73-77
Author(s):  
Jenny Sivakumar ◽  
Malliga Perumal
Keyword(s):  
Oryza Sativa ◽  
Toxic Metals ◽  
Inorganic Compounds ◽  
Industrial Effluents ◽  
High Strength ◽  
Microbial Reduction ◽  
Tannery Effluent ◽  
Tannery Effluents ◽  
Adequate Treatment ◽  
Industrial Complexes

Environmental pollution, especially of water bodies, is one of the major problems and it is increased day by day. The contamination of environment with various toxic metals is a serious threat for ecosystem and human health. Industrial effluents may contain toxic metals, harmful volatile compounds and several organic and inorganic compounds such as chromium, cadmium, mercury, arsenic and lead which are directly or indirectly discharged into the environment without adequate treatment. However, tannery industries are the major source of chromium contamination into the environment. Tanneries are typically characterized as pollution intensive industrial complexes which generate widely varying, high-strength wastewaters. The discharge of these toxic effluents has been a major loss from the ecological, social and economic perspective. There are many conventional physiochemical methods available for the removal of heavy metals from the tannery effluents that are highly expensive and require skilled techniques. However, microbial reduction of toxic chromium to non-toxic chromium by chromium resistance microorganism is the most pragmatic approach that offers an economical as well as eco-friendly process. Hence, this study examines the biodegradation of tannery effluent and its impacts on the germination of Oryza sativa seeds.

scholarly journals Chromate Reduction in Serratia marcescens Isolated from Tannery Effluent and Potential Application for Bioremediation of Chromate Pollution

2002 ◽  
Vol 2 ◽  
pp. 972-977 ◽  
Author(s):  
M.A. Mondaca ◽  
V. Campos ◽  
R. Moraga ◽  
C.A. Zaror
Keyword(s):  
Natural Waters ◽  
Waste Treatment ◽  
Environmental Concern ◽  
Electron Microscopic Examination ◽  
Tannery Effluent ◽  
Chromate Reduction ◽  
Bacterial Cells ◽  
Electron Microscopic ◽  
Treatment Processes ◽  
Transmission Electron

Pollution of aquatic systems by heavy metals has resulted in increasing environmental concern because they cannot be biodegraded. One metal that gives reason for concern due to its toxicity is chromium. Cr(VI) and Cr(III) are the principal forms of chromium found in natural waters. A chromate-resistant strain of the bacterium S. marcescens was isolated from tannery effluent. The strain was able to reduce Cr(VI) to Cr(III), and about 80% of chromate was removed from the medium. The reduction seems to occur on the cell surface. Transmission electron microscopic examination of cells revealed that particles were deposited on the outside of bacterial cells. A stable biofilm was formed in less than 10 h, reaching around 1010cfu attached per milligram of activated carbon. These findings demonstrate that immobilizedS. marcescensmight be used in industrial waste treatment processes.

scholarly journals Bioreduction of Cr(VI) by Viable Cells and Cell-Free Extract of a Moderate Halophile, Halomonas Smyrnensis KS802 and Evaluation of Their in Vitro Efficacy in Tannery Effluent

2020 ◽  
Author(s):  
Jhuma Biswas ◽  
AK Paul
Keyword(s):  
Reductase Activity ◽  
Basal Medium ◽  
Tannery Effluent ◽  
Chromate Reduction ◽  
Cell Free Extract ◽  
Tannery Effluents ◽  
Moderate Halophile ◽  
Viable Cells ◽  
Reducing Ability ◽  
Halomonas Smyrnensis

Abstract The present study was aimed to characterize the chromate reducing ability of cells and cell-free extract (CFE) of Halomonas smyrnensis KS802 (GenBank Accession No. KU982965) and evaluate their effectiveness in tannery effluents. Viable cells of the strain reduced 200 µM Cr(VI) in basal medium for halophiles (MH) in 10 h and was inversely proportional to Cr(VI) concentrations. The rapid reduction by cells (10⁹ cells/mL) was achieved with 7.5% NaCl, at pH 7 and 37°C which increased with increasing cell density (10¹° cells/mL). While acetate, Cu³⁺, Fe³⁺, SO₄²⁻, and CO₃²⁻ were stimulating the reduction, the inhibitors retarded the process significantly. The NADH-dependent chromate reduction of the CFE was found to be constitutive with Km and Vmax values of 56.58 µM and 3.37 µM/min/mg protein respectively. The optimal reductase activity of the CFE was evident at 200 µM Cr(VI), 10% NaCl, pH 8.0 and at 45°C. A higher concentration of CFE and electron donors increased the enzyme activity but was impacted negatively by toxic metals and anions. Both the cells and CFE were capable of reducing Cr(VI) remarkably from tannery effluent. FTIR and XRD spectra of chromate reducing cells confirmed possible complexation of reduced Cr-species with functional groups on cell surface.

Variabilities of Surface Water Quality of Degraded Post-mining Areas in Bytom

2021 ◽  
Vol 23 ◽  
pp. 318-331
Author(s):  
Aleksandra Czajkowska ◽  
Łukasz Gawor
Keyword(s):  
Water Quality ◽  
Surface Water ◽  
Ph Value ◽  
Surface Water Quality ◽  
Water Hardness ◽  
Total Content ◽  
Mining Area ◽  
Physicochemical Analysis ◽  
High Concentration ◽  
Mining Areas

In the paper there is presented an evaluation of variability of surface water quality (reservoirs and watercourses), on the area of degraded post-mining area in Bytom. The physicochemical analysis of water and compared with archival data obtained in 2009 and 2014. There were done analysis of following parameters: reaction, total content of substances dissolved in water, water hardness and the content of: Cl-, SO42-, HCO3-, Ca2+, Mg2+, K+, Na+, NH4+, NO3-, NO2-, PO4- ions as well as Fe and Mn. The examined surface waters were characterised by high content of solutes. Anions were dominated by chlorides, the sodium proved to be the dominating cation, the examined water samples were characterised by high concentration of sulphates. In all analyzed reservoirs, permissible concentrations of chlorides and sulphates were exceeded. In all sample points there was observed a decrease of pH value in long term period, the concentration of chlorides lowered, however concentrations of sulphates increased in the majority of sampling points.

The Influence of Amendment to the Red Mud and the Effects of Rehabilitated Red Mud on Sweet Sorghum Seedlings

2014 ◽  
Vol 1073-1076 ◽  
pp. 216-221
Author(s):  
Chong Jian Ma ◽  
Ravi Naidu ◽  
Hui Ming
Keyword(s):  
Red Mud ◽  
Ph Value ◽  
Environmental Concern ◽  
Physical Structure ◽  
Ecological Environment ◽  
In Situ Remediation ◽  
Seedling Root ◽  
Theoretical Significance ◽  
Alumina Industry

Red mud is the waste generated in alumina industry, which occupies much land and brings great harm to the ecological environment and its disposal and comprehensive utilization has become a major environmental concern of the whole society. This thesis discusses the rehabilitating effect of several amendments on red mud and their influences on the growth of the sorgo seedlings, eventually providing theoretical and technical support for in-situ remediation of the ecological environment red mud stacking area. The results show that the amendments could reduce pH value of the soil; the addition of phosphorus, gypsum and sludge can effectively reduce the pH value. All of these are beneficial to soil’s physical structure and seedling root. At the same time, sludge can provide necessary nutrients to seedlings while promote the metabolism. The research results have both theoretical significance and application prospect.

scholarly journals Isolation, Characterization, and Identification of Bacterial Population from Textile and Tannery Effluents of Bangladesh

2016 ◽  
Vol 29 (2) ◽  
pp. 84-88
Author(s):  
A Hakim ◽  
S Hoque ◽  
SM Ullah
Keyword(s):  
Bacterial Population ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Tannery Effluents ◽  
Bacterial Composition ◽  
Degrading Bacteria ◽  
Diagnostic Characteristics ◽  
Metal Treatment

Ten effluent samples from two different sites located at Hazaribagh tannery belt and Dhaka EPZ, Savar were collected. This study aimed to compare the bacterial composition isolated from tannery and textile effluents and to investigate the occurrence of metal toxicity tolerant and dye degrading bacteria and to select the potential strains for the use in bioremediation. The average bacterial count of HT and DETDE varied in between 3.35×106 and 5.45×106 cfu/mL and 4.8×106 and 7.75×106cfu/mL, respectively. A total of 12 bacterial isolates were characterized as strains of Bacillus, Staphylococcus, and Pseudomonas. A few, however, were re-cultured on other recommended media for verification of diagnostic characteristics. Maximum numbers of bacterial species were isolated from textile effluent. The results showed that a Gram-positive bacillus with a yellow pigment was considered as a major group of the population. Among them three isolates were identified based on alignments of partial sequence of 16S rRNA gene. These are also being used in different wastewater and metal treatment plants all over the world.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 84-88

Comparison of malachite green adsorption by two yeast strains using Raman microspectroscopy

2019 ◽  
Vol 366 (13) ◽  
Author(s):  
Jinghua Liu ◽  
Dongchen Zha ◽  
Xinyi Chen ◽  
Yin Wang ◽  
Zihang Wang ◽  
...  
Keyword(s):  
Malachite Green ◽  
Dye Removal ◽  
Environmental Concern ◽  
Health Hazard ◽  
Industrial Effluents ◽  
Raman Microspectroscopy ◽  
Operational Conditions ◽  
Yeast Strains ◽  
Batch Biosorption

ABSTRACT Malachite green (MG), as a triarylmethane compound, poses a health hazard and causes considerable environmental concern. In this work, batch biosorption experiments were conducted under different operational conditions such as pH, contact time and adsorption dose to assess the optimal parameters of MG dye removal by yeast biomass from aqueous solutions. Then, the conventional biochemical assay was used to evaluate MG removal efficiency (75.18 and 95.85%) by Saccharomyces cerevisiae and Candida utilis. In addition, Fourier-transform infrared spectroscopy in combination with Raman microspectroscopy was employed to scrutinize the differences of dye removal between two types of yeast strains. This study demonstrates that Raman microspectroscopy may serve as a useful and powerful tool to quantitatively measure the content of MG dye on yeast cell surfaces in situ, and even offer an alternative new technique to seek potentially proper adsorbents for the removal of toxic dyes from industrial effluents.

scholarly journals Detection of Tannery Effluents Induced DNA Damage in Mung Bean by Use of Random Amplified Polymorphic DNA Markers

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Abhay Raj ◽  
Sharad Kumar ◽  
Izharul Haq ◽  
Mahadeo Kumar
Keyword(s):  
Dna Damage ◽  
Mung Bean ◽  
Control Plant ◽  
Effluent Treatment ◽  
Treated Wastewater ◽  
Tannery Effluent ◽  
Genotoxic Effects ◽  
Tannery Effluents ◽  
Treated Effluent ◽  
Treated Effluents

Common effluent treatment plant (CETP) is employed for treatment of tannery effluent. However, the performance of CETP for reducing the genotoxic substances from the raw effluent is not known. In this study, phytotoxic and genotoxic effects of tannery effluents were investigated in mung bean (Vigna radiata (L.) Wilczek). For this purpose, untreated and treated tannery effluents were collected from CETP Unnao (UP), India. Seeds of mung bean were grown in soil irrigated with various concentrations of tannery effluents (0, 25, 50, 75, and 100%) for 15 days. Inhibition of seed germination was 90% by 25% untreated effluent and 75% treated effluent, compared to the control. Plant growth was inhibited by 51% and 41% when irrigated with untreated and treated effluents at 25% concentration. RAPD technique was used to evaluate the genotoxic effect of tannery effluents (untreated and treated) irrigation on the mung bean. The RAPD profiles obtained showed that both untreated and treated were having genotoxic effects on mung bean plants. This was discernible with appearance/disappearance of bands in the treatments compared with control plants. A total of 87 RAPD bands were obtained using eight primers and 42 (48%) of these showed polymorphism. Irrigating plants with untreated effluent caused 12 new bands to appear and 18 to disappear. Treated effluent caused 8 new bands and the loss of 15 bands. The genetic distances shown on the dendrogram revealed that control plants and those irrigated with treated effluent were clustered in one group (joined at distance of 0.28), whereas those irrigated with untreated effluent were separated in another cluster at larger distance (joined at distance of 0.42). This indicates that treated effluent is less genotoxic than the untreated. Nei’s genetic similarity indices calculated between the treatments and the control plants showed that the control and the plants irrigated with treated tannery effluent had a similarity index of 0.75, the control and plants irrigated with untreated 0.65, and between the treatments 0.68. We conclude that both untreated and treated effluents contain genotoxic substances that caused DNA damage to mung beans. CETP Unnao removes some, but not all, genotoxic substances from tannery effluent. Consequently, use of both untreated and treated wastewater for irrigation poses health hazard to human and the environment.

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