scholarly journals Phytochemical screening, antimicrobial activity and cytotoxicity of Nepalese medicinal plants Swertia chirayita and Dendrobium amoenum

2015 ◽  
Vol 3 (1) ◽  
pp. 48-57 ◽  
Author(s):  
Pritish Shrestha ◽  
Manisha Bista ◽  
Prativa Sharma ◽  
Shristi Shrestha ◽  
Basanta Lamichhane ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Swertia Chirayita ◽  
Percentage Yield

Research on medicinal plants are important to Nepal because most of its rural population relies on it as mode of medicine. Medicinal plants namely Swertia chirayita and Dendrobium amoenum were collected from mid hills of Nepal. The present study was undertaken to find the antimicrobial activity, phytochemical presence and their cytotoxicity in different extraction medium. The percentage yield from the plants were highest in warm methanol extraction with 12.6%, followed by ethyl acetate and lowest was for cold methanol. Plant extract showed the presence of antioxidants like alkaloid, terpenoids, flavonoids, tannin, glycosides. The Brine Shrimp Bioassay of methanol and ethyl acetate extract showed cytotoxicity. Chiraito extract showed LC50 of 199 ppm for Dhunche sample, 128.82 ppm for Daman sample and 131.82 ppm of Illam sample. The antibacterial activity of methanol extract of Chiraito and Dendrobium amoenum showed significant bioactivity by inhibiting growth of microbial species selected for the test. The zone of inhibition shown by the extracts was comparable to the standard antibiotics. Similarly, methanol extract of Chiraito also showed significant antifungal activity with the zone of inhibition comparable to amphotericin.Nepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 48-54

scholarly journals Antimicrobial activity and phytochemical screening of Adansonia digitata stem bark extract on some clinical isolates

2020 ◽  
Vol 22 (2) ◽  
pp. 035-044
Author(s):  
A.E. Ajiboye ◽  
S.O. Sadiq ◽  
M.R. Adedayo
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Clinical Isolates ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Adansonia Digitata ◽  
Standard Methods ◽  
E Coli

Adansonia digitata is a massive and deciduous tree with a height of about 24 m and of significant economic importance. The antimicrobial and  phytochemical screening of the aqueous and ethyl-acetate extract of stem bark of the plant were determined on some clinical isolates. The stem bark of the plant was collected and washed o properly before drying at 28 C. The pulverised stem bark was extracted with water and ethyl-acetate and screened for phytochemicals (qualitative and quantitative) using standard methods. The clinical isolates used were identified as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Candida albicans. The antimicrobial activities of the crude extracts were carried out using the agar well diffusion methods. The minimum inhibitory concentration (MIC), minimum bactericidal and fungicidal concentrations were carried out using standard methods. The aqueous extract exhibited a higher zone of inhibition against S. aureus (14.00 ± 0.57 mm) at a concentration of 200 mg/ml while a zone of inhibition of 11.66±0.33 mm was observed for E. coli using ethylacetate extract. Candida albicans had a zone of inhibition of 11.66±0.88 mm and 11.00±0.57 mm using aqueous and ethyl-acetate extracts respectively at 200 mg/ml. The MIC was 200 mg/ml for the crude extracts against the clinical isolates. The qualitative ethyl-acetate phytochemical screening revealed the presence of phenol, flavonoids, tannins, alkaloids, saponin and terpenoids. Phenol had the highest concentration of 2.02±0.25 mg/ml while terpenoids had a value of 1.38±0.02 mg/ml. Aqueous and ethyl-acetate extract of A. digitata possess significant antimicrobial activity against E. coli, S. aureus and C. albicans. However, K. pneumoniae showed resistance to ethyl acetate extract. Key words: Adansonia digitata, Antimicrobial activity, Clinical isolates, Phytochemical  screening

scholarly journals Antimicrobial, Antioxidant and Cytotoxic Activities of Callistemon citrinus (Curtis) Skeels

2012 ◽  
Vol 11 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Md Enamul Haque ◽  
Afrin Sultana ◽  
Biazid Alam Shibib ◽  
Md Mirazul Islam
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Stem Bark ◽  
Cytotoxic Activities

The petroleum ether, ethyl acetate and methanol extracts of the stem bark of Callistemon citrinus were subjected to screenings for antimicrobial and antioxidant activities and brine shrimp lethality. The ethyl acetate  extract and its column fractions XVIII and XIV exhibited moderate antimicrobial activity, while the methanol extract  revealed significant antioxidant activity having IC50 of 3.84 ?g/ml. The methanol extract and fraction-II showed  potent cytotoxic its cytotoxicity with the LC50 of 11.27 and 11.35 ?g/ml, respectively. DOI: http://dx.doi.org/10.3329/dujps.v11i1.12487 Dhaka Univ. J. Pharm. Sci. 11(1): 51-54, 2012 (June)

scholarly journals Chemical Composition, Phytochemical Screening and Antioxidant Activity of Acmella uliginosa (Sw.) Cass Leaves

2018 ◽  
Vol 16 (2) ◽  
pp. 162
Author(s):  
Askal Maimulyanti ◽  
Anton Restu Prihadi ◽  
Iwan Safrudin
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Percentage Yield ◽  
Phytochemical Constituents ◽  
Free Radical Scavenging Assay

Acmella uliginosa (Sw.) Cass is widely used in traditional medicine and used in the treatment of many diseases. Extraction of component in leaves of Acmella uliginosa used methanol, ethyl acetate and n-hexane solvent. The percentage yield of extract from the leaves in methanol, ethyl acetate and n-hexane were 6.50, 0.49, and 0.66, respectively. Analysis of component in various extracts of Acmella uliginosa (Sw.) Cass leaves used Gas Chromatography-Mass Spectrometry (GC-MS). The various classes of phytochemicals were identified from fractions of methanolic leaves extract by GC-MS. Qualitative analysis of phytochemical constituents in methanol, ethyl acetate and n-hexane extract were tannins, flavonoids, saponin, alkaloid and steroid. Antioxidant activity of Acmella uliginosa leaves from Indonesia was carried out by using 1,1,diphenyl-2-picryl-hydrazine (DPPH) free radical scavenging assay. The IC50 of three extract was calculated. A comparative study determined that Acmella uliginosa in ethyl acetate extract showed the highest antioxidant potential (IC50 = 28.09 µg/mL) compared to methanol extract (IC50 = 44.31 µg/mL) and n-hexane extract (181.23 µg/mL) against DPPH free radicals.

scholarly journals Antimicrobial activity of various extracts of Plectranthus ambionicus and Phyllanthus amarus

2016 ◽  
Vol 1 (2) ◽  
pp. 29 ◽  
Author(s):  
M. Pathmavathi ◽  
P. Thamizhiniyan
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Fungal Pathogens ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Phyllanthus Amarus ◽  
Zone Of Inhibition ◽  
Gram Positive Bacteria ◽  
Aspergillus Fumigates

Hexane, chloroform, ethyl acetate and methanol extracts of leaves of Plectranthus ambionicus and  Phyllanthus amarus were screened for their antimicrobial activity against human pathogenic bacterial and fungal strains. Antimicrobial activity was carried out by disc diffusion method, determination of minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC) and minimum fungicidal concentrations (MFC) against four strains of Gram positive bacteria, three strains of Gram negative bacteria and three species of fungi. The antimicrobial activity of various extracts of Plectranthus ambionicus and Phyllanthus amarus showed varied levels of antimicrobial activity against the studied bacterial and fungal pathogens. The mean zone of inhibition produced by all the tested extracts ranged from 7.0 ± 0.50 mm to 29.5 ± 0.50 mm. The MIC, MBC and MFC values were between 15.62 and 1000 μg/mL. The ethyl acetate extract of Plectranthus ambionicus showed good antimicrobial activity with the highest mean zone of inhibition (29.5±0.5 mm), lowest MIC (15.62 μg/mL) and MBC (31.25 μg/mL) values followed by ethyl acetate extract of Phyllanthus amarus(23.6±0.50 mm; MIC=62.5; MBC=125 μg/mL) against Aspergillus fumigates.

scholarly journals Antimicrobial activity, toxicity and phytochemical screening of selected medicinal plants of Losho, Narok County, Kenya

2017 ◽  
Vol 15 (1) ◽  
pp. 29-43
Author(s):  
DUNCAN MUTISO CHALO ◽  
CATHERINE LUKHOBA ◽  
DOSSAJI SAIFUDDIN FIDAHUSSEIN ◽  
JOSEPH MWANZIA NGUTA
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Brine Shrimp ◽  
Inhibition Zone ◽  
Phytochemical Screening ◽  
Methicillin Resistant ◽  
Phytochemical Composition ◽  
Crude Extracts ◽  
Broth Microdilution Method ◽  
Organic Extracts

Chalo DM, Lukhoba C, Fidahussein DS, Nguta JM. 2017. Antimicrobial activity, toxicity and phytochemical screening of selected medicinal plants of Losho, Narok County, Kenya. Biofarmasi (Rumphius J Nat Prod Biochem) 15: 29-43. In Kenya, microbial infections are a major cause of morbidity. The effectiveness of antibiotic is threatened by the increase of resistance of pathogenic microbes against most available drugs because new pathogens continue to emerge. Nowadays, herbal remedies offer hope since they are readily available and cheap. The aim of this research was to investigate the activity of antimicrobial, the lethality of brine shrimp and phytochemical composition of crude extracts of four selected plants namely Schrebera alata (Oleaceae), Ormocarpum kirkii (Papilionoideae), Helichrysum forskahlii (Asteraceae) and Cussonia holstii (Araliaceae) that are medicinally used by herbalists from Losho, Narok County Kenya for treatment of ear, nose and throat infections, gastrointestinal disorders and skin diseases. Qualitative antimicrobial susceptibility test against five microorganisms, methicillin-resistant Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans was investigated using agar diffusion methods to produce inhibition zones and the data accrued were analyzed using Analysis of variance. Minimum inhibitory concentrations were determined by broth microdilution method. Toxicity of the extracts was analyzed using brine shrimp lethality assay. The median fatal concentration of fifty was determined by data analysis using Finney‟s computer program. Phytochemical screening for flavonoids, sterols, alkaloids, tannins, quinones and terpenoids and saponins was determined using standard procedures. The observation showed that the organic crude extracts of H. forskahlii had the highest inhibition zone against methicillin-resistant S. aureus of 19.5 and 18.5 mm in agar well and agar disk diffusion respectively. In addition, organic extracts of H. forskahlii showed the highest antifungal inhibition zone of 8.5 mm in agar well diffusion. Minimum values of inhibitory concentrations varied from 15.625 to 250 mg/mL. Organic crude extracts of H. forskahlii and C. holstii were found to be highly toxic with lethal concentration of 0.009 mg/mL. All plant crude extracts contained flavonoids, sterols, alkaloids, tannins, quinones, and terpenoids. Saponins were present in all the plant extracts except in organic extracts of H. forskahlii. This study promoted the first record of antimicrobial activity, toxicity and phytochemical composition of S. alata and C. holstii. The study has shown that H. forskahlii and O. kirkii possess promising antimicrobial activity against microbes of health importance and could lead to the isolation of new, safe and efficacious antimicrobial compounds. Further research should be carried on O. kirkii and S. alata to isolate and characterize the compounds responsible for the observed activity.

In Vitro Antimicrobial Activity of 80% Methanol Extract and Solvent Fractions of Urtica simensis Hochst. ex. A. Rich. (Urticaceae) Leaves against Pathogenic Bacteria and Fungi

2021 ◽  
Vol 36 (2) ◽  
pp. 97-108
Author(s):  
Fentaye Kassa ◽  
Teshome Nedi ◽  
Aklilu Feleke ◽  
Tadesse Eguale ◽  
Haile Alemayehu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Fungal Species ◽  
Diffusion Method ◽  
Active Fraction ◽  
Gram Positive ◽  
Aqueous Fraction ◽  
Gram Positive Bacteria

Medicinal plants have been used for the treatment of infectious diseases since a long time. Urtica simensisHochst. ex. A. Rich. is one of the endemic medicinal plants used for the treatment of bacterial and fungalinfections in Ethiopian folklore medicine. The aim of this study was to investigate the antimicrobial activityof 80% methanol extract and solvent fractions of the leaves of U. simensis. The 80% methanol extract wasprepared by maceration and the solvent fractions were obtained by successive fractionation of the 80%methanol extract with chloroform, ethyl acetate, n-butanol and distilled water. The antimicrobial activity ofthe extracts was evaluated on eight bacterial and two fungal species using agar well diffusion method atconcentrations of 200, 400 and 800 mg/ml. Minimum inhibitory concentrations (MICs) were determined bybroth microdilution method. From the gram positive bacteria tested, the most susceptible species wasStreptococcus pneumoniae (MIC of 1.36 mg/ml), while Klebsiella pneumoniae and Aspergillus niger weremost susceptible gram negative bacterial and fungal species with MIC values of 2.54 and 3.13 mg/ml,respectively. The least values of MIC, MBC and MFC were 1.36, 5.21 and 6.25 mg/ml, respectively. Resultsof the present study revealed that the 80% methanol extract and solvent fractions, of the leaves of U.simensis, with the exception of the aqueous fraction, possess activity against the growth of selected pathogenic bacterial and fungal species. Among the solvent fractions, the n-butanol fraction was the most active fraction against the gram positive bacteria tested, while the ethyl acetate fraction was the most active fraction against the other tested organisms. Further studies are needed to isolate the active principles, elucidate the mechanism of antimicrobial action, determine in vivo antimicrobial activity and effect of the extracts on other pathogens including clinical isolates.

A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS

2016 ◽  
Vol 9 (5) ◽  
pp. 259 ◽  
Author(s):  
Sumathy Rengarajan ◽  
Vijayalakshmi Melanathuru ◽  
Deecaraman Munuswamy ◽  
Sankaranarayanan Sundaram ◽  
Saravanan Thiruverkadu Selvaraj
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methanol Extract ◽  
Phytochemical Screening ◽  
Layer Chromatography ◽  
Indian Medicinal Plants

ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.

scholarly journals A STUDY ON PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL AND α-ΑMYLASE INHIBITORY ACTIVITIES OF CRUDE EXTRACTS OF THE STEM BARK OF PISONIA GRANDIS, R.BR

2017 ◽  
Vol 10 (11) ◽  
pp. 129
Author(s):  
Vishu Mohan ◽  
Mohan Kumar Ramasamy ◽  
Srikalyani Vemuri ◽  
Ilango Kaliappan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

  Objective: The aim of the present study is to determine the phytochemical screening, antioxidant activity and α-amylase inhibitory activity of the crude hexane, ethyl acetate and ethanolic stem bark extract of Pisonia grandis.Methods: The evaluation of antioxidant and antimicrobial activity, total phenolic, and flavonoid content were assessed using 2,2-diphenyl-1- picrylhydrazyl, Folin–Ciocalteu’s reagent, and aluminum chloride assay, respectively. The antidiabetic activity was assessed for porcine pancreatic α-amylase for the stem bark of P. grandis. Results: Phytochemical screening confirmed the presence of phenolic, flavonoids, tannins, saponins, terpenoids, and steroids in all the three extracts. The antioxidant activity showed 148.2 μg/ml, total phenolic content (gallic acid equivalent), 0.0665±0.0002 mg/g, flavonoid content (quercetin equivalent), 0.6061±0.1817 mg/g, and inhibitory concentration 50% values were found to be 40.42 μg/ml and showed better in ethyl acetate extract. The antidiabetic activity exhibited mimic action with insulin due to the presence of pinnatol in the stem bark and leaves of P. grandis. Conclusion: P. grandis stem bark crude ethyl acetate extract showed strong antioxidant activity, high phenolic, and flavonoid content. The antimicrobial activity was studied in both Gram-positive and Gram-negative strains against ampicillin and rifampicin as reference drugs. Antidiabetic activity shows effective result by α-amylase inhibitory activity.

scholarly journals ISOLASI DAN ELUSIDASI STRUKTUR SENYAWA TRITERPENOID DARI KULIT BATANG AMBACANG (Mangifera foetida L.) SERTA UJI BRINE SHRIMP LETALITY TEST (BSLT)

2015 ◽  
Vol 9 (1) ◽  
pp. 1 ◽  
Author(s):  
Adlis Santoni ◽  
Sabariah ◽  
Mai Efdi
Keyword(s):  
Ethyl Acetate ◽  
1H Nmr ◽  
13C Nmr ◽  
Brine Shrimp ◽  
Spectroscopic Data ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Toxic Effects ◽  
Toxic Compound ◽  
Isolated Compound

A brine shrimp toxic compound has been isolated from Mangifera foetida L. steam bark. Based on spectroscopic data (IR,1H- NMR, 13C-NMR 1D and 2D), the structure of this compound was identified as mangiferenes B, C30H47O2. In this study, the n-hexane, ethyl acetate, methanol extracts and isolated compound showed toxic effects (LC50 471.9543; 534.5209, 678.4421, and 22.1615 μg/mL, respectively). The toxicity degree among the three extracts and isolated compound is isolated compound > n-hexane extract > ethyl acetate extract > methanol extract.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAN SKRINING FITOKIMIA EKSTRAK HEKSANA, ETIL ASETAT DAN METANOL TUMBUHAN SURUHAN (Peperomia pellucida L. Kunth)

2020 ◽  
Vol 16 (2) ◽  
pp. 105-111
Author(s):  
Restianti Restianti ◽  
Bimo Budi Santoso ◽  
Maria Ludya Pulung
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
E Coli ◽  
Activity Test ◽  
Solvent Analysis

Antibacterial activity test and screening of phytochemical of extract hexane, ethyl acetate  and methanol have been carried out. The extraction of the P.pellucida plants was carried out by the maceration method in stages based on the polarity of the solvent. Analysis of chemical compounds done with phytochemical screening test. Based on the identification of chemical compounds by phytochemical screening, hexane and ethyl acetate extract has been detected only in the presence of flavonoid compounds with moderate categories. Methanol  extract contains high-grade alkaloids and moderate flavonoid and tannin compounds. Antibacterial test using disffusion methods suggest that ethyl acetate and methanol extract have categorically strong antibacterial activity againts the S. aureus  of 14 mm and 16 mm respectively. Hexane extract and acetate ethyl have a strong inhibition to  E. coli  of 11 mm and 12 mm respectively. Methanol extract has a weak inhibition to bacteria E. coli  and a hexane extract has a weak inhibition to bacteria S. aureus.

Sign in / Sign up

Export Citation Format

Share Document