scholarly journals Potential Antibacterial Activity of Bergenia purpurascens

2012 ◽  
Vol 12 ◽  
pp. 157-162 ◽  
Author(s):  
Gan B Bajracharya ◽  
Rokesh Maharjan ◽  
Bijaya L Maharjan
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Salmonella Typhi ◽  
Traditional Use ◽  
Conservation Area ◽  
Healing Of Wounds ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
And Staphylococcus Aureus

Bergenia purpurascens, an important traditional medicinal plant used as Ayurveda, was collected at 3800 m from the Manaslu Conservation Area. In vitro evaluation of the crude extracts of the plant rhizomes using agar well diffusion assay against eight bacteria displayed potential antibacterial activity. The minimum inhibitory concentration values for Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella paratyphi, Salmonella typhi, Salmonella typhimurium, Shiegella flexneri and Staphylococcus aureus were determined. This study supports the traditional use of the plant material for the healing of wounds and antibiotic effect.DOI: http://dx.doi.org/10.3126/njst.v12i0.6494 Nepal Journal of Science and Technology 12 (2011) 157-162 

scholarly journals Estimation of Alkaloids and Antibacterial Activity of Aconitum spicatum Bruhl Stapf from Manaslu Conservation Area

2013 ◽  
Vol 13 (1) ◽  
pp. 67-71
Author(s):  
Sajan L Shyaula ◽  
Ash B Ngakushi ◽  
Bijaya L Maharjan ◽  
Mangala D Manandhar
Keyword(s):  
Antibacterial Activity ◽  
Salmonella Typhi ◽  
Klebsiella Pneumonia ◽  
Conservation Area ◽  
National Priority ◽  
Pathogenic Microbes ◽  
The Government ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

Aconitum spicatum is one of the deadly poisonous and highly valued medicinal plant. It is a national prioritized herb among 30 national priority herbs listed by the government of Nepal for their development, research and cultivation. The acute toxicity of the extract of this plant appeared to be directly related to the alkaloid content. The amount of total alkaloid of A. spicatum collected from Manaslu conservation area was calculated to be 1.7% by spectrophotometric method based on Dragendorff’s reagent. In-vitro evaluation of the crude extract of plant rhizomes using agar well diffusion assay against eight bacteria, Staphylococcus aureus, Escherichia coli, Schigella flexneri, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi, Salmonella typhimurium and Klebsiella pneumonia, displayed potential antibacterial activity. The diethyl ether fraction was the most effective against all pathogenic microbes with minimum bacterial concentration value 3.125-6.25 mg/ml. Nepal Journal of Science and Technology Vol. 13, No. 1 (2012) 67-71 DOI: http://dx.doi.org/10.3126/njst.v13i1.7443

scholarly journals Antibacterial Activity of Ethanolic Extract from Tempuyung Leaf (Sonchus arvensis L.) againts Salmonella typhi by In Vitro Study

2016 ◽  
Vol 2 (2) ◽  
pp. 1
Author(s):  
Rinda Yanuarisa ◽  
Dini Agustina ◽  
Ali Santosa
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Control Group ◽  
Natural Material ◽  
Control Group Design ◽  
Sonchus Arvensis

Tifoid fever caused by Salmonella typhi (S. typhi) is endemic disease in Indonesia. S. typhi begin to resist to chloramphenicol, so it is necessary to study about natural material as traditional medicine such as tempuyung. The aim of this research was to study antibacterial activity and Minimum Inhibitory Concentration (MIC) of ethanolic extract from tempuyung leaf (Sonchus arvensis L.) againts growth of S. typhi. This research used quasy experimental methods with post test only control group design. Treatment consisted of 8 concentrations with 5 replications was 2,5 µg/disc, 5 µg/disc, 10 µg/disc, 20 µg/disc, 30 µg/disc, 40 µg/disc, 60 µg/disc, and 80 µg/disc. As much as 10 µl from each concentration was dropped into disc and placed in Mueller Hinton Agar. Then incubated for 18 hours to 37oC. Observation of bacterial inhibition zone by using a caliper. Result of this research that the leaf extract at a concentration 10 µg/disc, 20 µg/disc, 30 µg/disc, 40 µg/disc, 60 µg/disc, and 80 µg/disc can inhibit the growth of Salmonella typhi. Minimum Inhibitory Concentration of ethanolic extract from tempuyung leaf was 10 µg/disc qualitatively and 4,43 µg/disc quantitatively. Key words: Sonchus arvensis L., Salmonella typhi, antibacterial

Ferrocene-Based Antibacterial Agents: Synthesis, Characterization, and Biological Evaluation of Metal (II) Complexes with Glycine-Derived Compounds

2011 ◽  
Vol 339 ◽  
pp. 317-320 ◽  
Author(s):  
Yu Ting Liu ◽  
Xiao Ming Sun ◽  
Da Wei Yin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agents ◽  
Salmonella Typhi ◽  
Biological Evaluation ◽  
Bacterial Strains ◽  
Gram Negative ◽  
And Staphylococcus Aureus

A series of Ferrocene Schiff bases derived from glycine and their cobalt (II), copper (II), nickel (II), and zinc (II) metal complexes have been synthesized and characterized by IR, 1H NMR, MS and elemental analysis, the results conformed well with expected structures. The synthesized ligands, along with their metal (II) complexes, were screened in vitro for their antibacterial activity against three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) and two Gram-positive (Bacillus subtilis and Staphylococcus aureus) bacterial strains. The results showed that the metal (II) complexes are more potent in antibacterial activity against one or more species in comparison with those of uncomplexed ligands.

Ferrocene-Based with Thiadiazole Antibacterial Agents: Synthesis, Characterization, and Biological Evaluation

2012 ◽  
Vol 189 ◽  
pp. 181-184
Author(s):  
Da Wei Yin ◽  
Xiao Ming Sun ◽  
Yu Ting Liu
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Agents ◽  
Salmonella Typhi ◽  
Biological Evaluation ◽  
New Drugs ◽  
Bacterial Strains ◽  
Gram Negative ◽  
And Staphylococcus Aureus

A series of ferrocene schiff bases derived from Thiadiazole have been synthesized and characterized by IR, 1H NMR, and elemental analysis, the results conformed well with expected structures. The synthesized compounds were screened in vitro for their antibacterial activity against three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) and two Gram-positive (Bacillus subtilis and Staphylococcus aureus) bacterial strains. The results showed that these compounds are a series potential new drugs in antibacterial activity against one or more species.

scholarly journals In-vitro antibacterial activity of the seed extract of three-member Artocarpus towards Methicillin-Resistant Staphylococcus aureus (MRSA)

2020 ◽  
Vol 9 (2) ◽  
pp. 128-135
Author(s):  
Muhammad Evy Prastiyanto ◽  
Inas Hasna Azizah ◽  
Hafizha Dara Haqi ◽  
Bagus Dwi Yulianto ◽  
Aulia Bella Agmala ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Seed Extract ◽  
Methicillin Resistant ◽  
Biological Source ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Seed Extracts

Methicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from a biological source. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden, and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method and minimum bactericidal concentration. The antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member Artocarpus showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of A. heterophyllus at 62.5 mg/mL, A. champeden at 31.25 mg/mL, A. camansi at 250 mg/mL. All seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of Artocarpus as a source of natural antibacterial.

scholarly journals Antibacterial activity of Brazilian Northeast plants against Corynebacterium pseudotuberculosis

2021 ◽  
Vol 10 (11) ◽  
pp. e509101119875
Author(s):  
Maria Emília Alcântara ◽  
Antonio Pedro Fróes de Farias ◽  
Maria da Conceição Aquino de Sá ◽  
Soraya Castro Trindade ◽  
Roberto Meyer ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Small Ruminants ◽  
Inhibition Zone ◽  
Corynebacterium Pseudotuberculosis ◽  
High Prevalence ◽  
In Vitro Antibacterial Activity ◽  
Agar Well Diffusion Assay ◽  
Brazilian Northeast ◽  
Diffusion Assay

The production of small ruminants is an important economic activity of the brazilian Northeast, but some diseases have a high prevalence in this region, such as caseous lymphadenitis (CL), caused by Corynebacterium pseudotuberculosis. The treatment of CL is often ineffective, which justifies the search of new active principles from plants, mainly of the region, to have an accessible treatment. For this reason, the present study was carried out to evaluate the in vitro antibacterial activity of Annona squamosa, Azadirachta indica, Allium sativum, Prosopis juliflora and Portulaca oleracea against C. pseudotuberculosis. Agar well diffusion assay (AWD) and broth microdilution - to determine minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) - evaluated the antimicrobial activity. The highest antibacterial potential was obtained by ethanolic extracts of A. indica leaf (MIC 0,12 mg/mL for 2 strains), A. squamosa stalk (MIC 0,55 mg/mL for 3 strains) and shell (MIC 0,6 mg/mL for 3 strains). These extracts also presented the highest inhibition zone in AWD (30 mm, 38 mm and 32 mm, respectively). A. squamosa and A. indica have high antimicrobial potential against C. pseudotuberculosis.

scholarly journals Antibacterial Activity of Eel (Anguilla spp.) Mucus against Salmonella typhi

2016 ◽  
Vol 8 (3) ◽  
pp. 179 ◽  
Author(s):  
Tomy Nurtamin ◽  
Resty Yulianita Nurman ◽  
Indria Hafizah
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Typhoid Fever ◽  
Inhibitory Concentration ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
In Vitro Test ◽  
Fish Mucus ◽  
Vitro Test

BACKGROUND: Typhoid fever has become one global health problem. Typhoid fever is caused by a Gram-negative bacterium, Salmonella typhi. Eel (Anguilla spp.) is a fish which lives in the sea or in freshwater. Several previous studies have found that Anguilla spp. mucus has the ability as antibacterial against Gram-positive and negative. Although the antibacterial activity of Anguilla spp. against various pathogens had been reported, very little is known about its activity against Salmonella typhi. The aim of this study was to investigate antibacterial activity of Anguilla spp. mucus against Salmonella typhi bacteria.METHODS: Present study was an experimental in vitro test. Antibacterial activity assays were carried out by the disc diffusion method. Antibacterial activity was determined by the clear zone formed around the paper disc and minimum inhibitory concentration was determined by observing the lowest concentration which could inhibit the growth of Salmonella typhi.RESULTS: Result of the present study showed that the Anguilla spp. mucus has inhibitory effects against Salmonella typhi. Minimum inhibitory concentration from the Anguilla spp. mucus was 12.5%.CONCLUSION: Anguilla spp. mucus has antibacterial activity against the Salmonella typhi bacteria.KEYWORDS: eel fish mucus, Anguilla spp., antibacterial activity, Salmonella typhi

scholarly journals An Investigation on the Pharmacological Profile of Titanium (IV) and Aluminum (III) 8-Hydroxyquinoline Derivatives Grafted on MCM-41 Mesoporous Silica

2018 ◽  
Vol 3 (3) ◽  
pp. 120-126
Author(s):  
Yousef Fazaeli ◽  
Gholamreza Gholamreza Shahhosseini ◽  
Samira Samira Shahbazi ◽  
Shahzad Feizi
Keyword(s):  
Mesoporous Silica ◽  
Salmonella Typhi ◽  
Assay Method ◽  
Escherchia Coli ◽  
Chlorine Substituent ◽  
Agar Well Diffusion Assay ◽  
Anti Fungal ◽  
Diffusion Assay ◽  
And Staphylococcus Aureus ◽  
Mcm 41

8-hydroxyquinoline and 5-chloro-8-hydroxyquinoline were treated with a titanium (IV) and Aluminum (III) alkoxide reagents to generate (Q)2(2-BuO) Al (Q = 8-hydroxyquinoline and 5-chloro-8-hydroxyquinoline) and (Q)2(O-iPr)2Ti (Q = 5-chloro-8-hydroxyquinoline) complexes. These active complexes underwent successive grafting on MCM-41 mesoporous silica in order to improve overall toxicity and stability of them. The prepared nanocomposites were characterized. Anti-bacterial activity of the compounds on Escherchia coli (ATCC 1330), Salmonella typhi (PTCC 1609) and Staphylococcus aureus subsp. Areas (PTCC 1113) were tested and the anti-fungal activity of them was determined using the agar well diffusion assay method on SCC (for yeasts) and Muller Hinton Agar medium (for filamentous fungi). The results showed that the anti-microbial (bacterium and fungi) effect of the Ti & Al complexes can be improved in the solid-state using appropriate chlorine substituent on the 8-hydroxyquinoline ligand and grafting to the mesoporous silica.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

scholarly journals A comparison of the antibacterial activity of some African black soaps and medicated soaps commonly used for the treatment of bacteria-infected wound

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Olufunmiso O. Olajuyigbe ◽  
Morenike O. Adeoye-Isijola ◽  
Otunola Adedayo
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Waste Product ◽  
Antibacterial Activities ◽  
Traditional Use ◽  
E Coli ◽  
African Black ◽  
Soap Production ◽  
Inhibition Zones ◽  
Better Than

Background: Black soap is a medicinal product that could be harnessed for economic purpose if properly packaged, and misconception about its traditional use by herbalists is thrown overboard.Aims: To promote the relevance of these soaps for economic development, this study compared the antibacterial activity of black soaps with medicated soaps widely used against bacterial infections.Methods: The antibacterial activities of these soap samples were determined by agar diffusion and macrobroth dilution methods.Results: In this study, the statistical analysis of the inhibition zones showed that black soaps were significantly (p < 0.05) more active than medicated soaps used against the test bacterial isolates. The black soaps inhibited and killed the isolates better than the medicated soaps at the different concentrations used. The minimum inhibitory concentration for Klebsiella pneumoniae and Enterococcus faecalis ranged between 0.125 mg/mL and 2 mg/mL, Staphylococcus aureus (0.25–4) mg/mL, Escherichia coli (0.125–4) mg/mL and Pseudomonas aeruginosa (1–4) mg/mL. The result showed that K. pneumoniae and E. faecalis were the most susceptible, followed by E. faecalis > E. coli > S. aureus > P. aeruginosa.Conclusion: As a valuable medicinal output derivable from organic waste product that could be converted to wealth, African black soap production, utilisation and commercialisation have tremendous economic potentials. These soaps showed significant antibacterial activity greater than those of the medicated soaps. Hence, their use could be a better option in place of commercially available medicated and antiseptic soaps because of the degree of antibacterial activities they exhibited.

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