scholarly journals Cressa cretica Pharmacognosy, and Pharmacology (A review )

2021 ◽  
Vol 30 (2) ◽  
pp. 31-40
Author(s):  
Noor Sabah Jaafar ◽  
Iman S Jaafar ◽  
Zainab S Noori
Keyword(s):  
Molecular Docking ◽  
Ethyl Acetate ◽  
Phenolic Acid ◽  
Clinical Studies ◽  
Ethyl Acetate Fraction ◽  
Antiviral Effect ◽  
Docking Studies ◽  
Methanolic Extracts ◽  
Dicaffeoylquinic Acid ◽  
Oil Source

  Cressa cretica (Shuwwayl) is a halophytic that belongs to Convolvulaceae, naturally grown in the Middle East including Iraq. Traditionally the plant is used as a paste for sore treatment, also it is used for fever, jaundice, and other illness. Regarding nonclinical use it is used as goat, sheep, and camel feed also as an oil source. Flavonoids including quercetin, kamepferol, apigenin, and their glycosides, phenolic acid as chlorogenic acid, and phytosterols mainly ?–sitosterol were the most important phytochemicals that were detected in this halophyte. Crude ethanolic, methanolic extracts and ethyl acetate fraction of the areal parts were used in clinical studies and demonstrated various effects as hepatoprotective, cytotoxic, and genotoxic effect. In molecular docking studies, 3,5-dicaffeoylquinic acid showed antiviral effect vs SARS-CoV-2 (sever acute respiratory syndrome corona virus-2). The purpose of this review was to clarify and discuss all aspects regarding Cressa cretica.

scholarly journals Inulae Flos and Its Compounds Inhibit TNF-α- and IFN-γ-Induced Chemokine Production in HaCaT Human Keratinocytes

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Jung-Hoon Kim ◽  
Hye-Sun Lim ◽  
Hyekyung Ha ◽  
Chang-Seob Seo ◽  
Hyeun-Kyoo Shin
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Hacat Cell ◽  
Human Keratinocytes ◽  
Ethyl Acetate Fraction ◽  
The Other ◽  
Hacat Cells ◽  
Chemokine Production ◽  
Dicaffeoylquinic Acid ◽  
Dependent Inhibition

The present study is to investigate which kinds of solvent extracts of Inulae Flos inhibit the chemokine productions in HaCaT cell and whether the inhibitory capacity of Inulae Flos is related with constitutional compounds. The 70% methanol extract showed comparatively higher inhibition of thymus and activation-regulated chemokine (TARC/CCL17) in HaCaT cells, therefore this extract was further partitioned with n-hexane, chloroform, ethyl acetate, butanol, and water. The ethyl acetate fraction inhibited TARC, macrophage-derived chemokine (MDC/CCL22), and regulated on activation of normal T-cell-expressed and -secreted (RANTES/CCL5) production in HaCaT cells better than the other fractions. The compounds of Inulae Flos, such as 1,5-dicaffeoylquinic acid and luteolin, inhibited TARC, MDC, and RANTES production in HaCaT cells. 1,5-Dicaffeoylquinic acid was contained at the highest concentrations both in the 70% methanol extract and ethyl acetate fraction and inhibited the secretion of chemokines dose-dependently more than the other compounds. Luteolin also represented dose-dependent inhibition on chemokine productions although it was contained at lower levels in 70% methanol extract and solvent fractions. These results suggest that the inhibitory effects of Inulae Flos on chemokine production in HaCaT cell could be related with constituent compounds contained, especially 1,5-dicaffeoylquinic acid and luteolin.

scholarly journals Antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers towards Streptococcus sanguis ATCC 10566

2018 ◽  
Vol 30 (3) ◽  
pp. 190
Author(s):  
Fajar Fatriadi ◽  
Dikdik Kurnia ◽  
Mieke Hemiawati Satari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Chlorhexidine Gluconate ◽  
Streptococcus Sanguis ◽  
Microdilution Method ◽  
Methanolic Extracts ◽  
Natural Remedies

Introduction: Streptococcus sanguis is an initial cause of dental plaque formation which is the initial cause of caries. One of the preventive treatments can be done by using the mouthwash containing antibacterial substances. Along time, natural remedies are proven to be having more antibacterial properties. Ant-plant (Myrmecodia pendens Merr. & Perry) tubers are types of epiphytic plant that grows in many parts of Papua, with many health benefits, and are known to contain flavonoids, tannins, and tocopherols. This study was aimed to determine the antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers on the growth of Streptococcus sanguis ATCC 10566. Methods: The ant-plant tubers were extracted with soxhletation method using the methanol solvent. The fractionation was then performed using ethyl acetate to obtain the ethyl acetate fraction. Result: The phytochemical test showed that the ethyl acetate fraction of the ant-plant contained phenolics, tannins, flavonoids, and terpenoids. The bacterial test in this study was using the microdilution method using the ELISA Reader by measuring the Minimum Inhibitory Concentration (MIC) of ethyl acetate fraction of ant-plant tubers towards Streptococcus sanguis with positive control was using the chlorhexidine gluconate as the golden standard medication. The ethyl acetate fraction of ant-plant tuber had an antibacterial effect towards Streptococcus sanguis in the MIC of 31.25 ppm, while chlorhexidine gluconate was 0.49 ppm. Conclusion: The ethyl acetate fraction of ant-plant had a lower antibacterial activity compared to chlorhexidine gluconate on the growth of Streptococcus sanguis ATCC 10566.Keywords: Ant-plant tubers, Myrmecodia pendens Merr. & Perry, antibacterial activity, Streptococcus sanguis ATCC 10566.

scholarly journals Separation and Identification of Phenolic Acid from Borago officinalis (F:Boraginaceae) Cultivated in Iraq

2020 ◽  
Vol 29 (2) ◽  
pp. 139-151
Author(s):  
Ashwaq T Kareem ◽  
Maha N. Hamad
Keyword(s):  
Ethyl Acetate ◽  
Phenolic Acid ◽  
Inflammatory Diseases ◽  
Analytical Techniques ◽  
Ethyl Acetate Fraction ◽  
Extraction Methods ◽  
Absolute Methanol ◽  
Phytochemical Screening ◽  
Borago Officinalis ◽  
Active Constituents

    The plant Borago officinalis, which belongs to the Boraginaceae family and Celebrated as borage, is one of the useful medicinal plants cultivated in Iraq. It was used in olde medicine in Iraq, Irane, Syria and Europe for management of various diseases. It is commonly used as an atonic, tranquilliser, management of cough, sore throat, pneumonia, swelling, inflammatory diseases, antioxidant, and anticancer. This project provides the first comprehensive research done in Iraq to study the phytochemicals and the methods of extraction and isolation of active constituents from Borago officinalis cultivated in Iraq. The plant was harvested in spring from AL-Rifai, Nassiriyah city, IRAQ in February 2019.were washed carefully, dried under dark, and milled in a mechanical grinder to a fine powdere then measure the weight of the plant.The plant was extracted by cold extraction methods using (85%absolute methanol ) solvent for three days then Fractionation was done by petrollium ethere, chloroform, ethyl acetate and n-butanol(n.b) to separate the active constituentse according  to the change in polaritiese. The phytochemical screening exposed the presence of, phenols, tannins, fatty acid, in the plant's Ethyl acetate fraction, n-butanol fraction was usedfor identification and isolationof phenolic compoundsby by TLC, PLC HPLC andLC/mass. The Phenolic acid (Sinapic acide, Rosmarinic acides,Caffeic acid) were seperated and purified by PLC. The isolated compounds were subjected to several chemical chromatographic and spectral analytical techniques for their identification, such as TLC, PLC, HPLC, UV, and  LC/mass.

scholarly journals Chemical Composition and Antimycoplasmic Activity of Eugenia mattosii Leaves, Stems and Isolated Compounds

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400 ◽  
Author(s):  
Giovana Vechi ◽  
Adrielli Tenfen ◽  
Ariela Maína Boeder ◽  
Lorena Hernandez-Gómez ◽  
Caio Maurício Mendes de Córdova ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Ethyl Acetate ◽  
Cell Walls ◽  
Ethyl Acetate Fraction ◽  
Chloroform Fraction ◽  
Bioactive Substances ◽  
Methanolic Extracts ◽  
Biological Potential ◽  
Activity Quantification ◽  
Human And Animal Diseases

The Eugenia genus is rich in bioactive substances with biological potential. Mollicutes are bacteria without cell walls, which are responsible for various human and animal diseases. The aim of this study was to evaluate the chemical composition and antimollicute activity of Eugenia mattosii. Leaves and stems were extracted with methanol, partitioned into fractions of different polarities, and submitted to column chromatography in order to isolate major compounds. Identification and quantification of isolated substances in extracts and fractions were performed by UPLC-PDA/ESI+ - QTOF. Methanolic extracts, ethyl acetate and chloroform fractions and isolated substances were screened for antimycoplasmic activity against five Mycoplasma strains. The methanolic extracts of leaves and stems showed moderate antimycoplasmic activity (MICs = 250-500 μg.mL-1). The fractions exhibited better effect, with MICs = 125-1000 μg.mL-1, especially the ethyl acetate fraction of the leaves, which presented MICs of 125-250 μg.mL-1 for all strains tested. Phytochemical analyses evidenced the presence of some phenolic compounds, including pinostrobin, cryptostrobin and catechin, the first showing promising antimycoplasmic activity. Quantification of the compounds demonstrated higher concentrations of pinostrobin and cryptostrobin in the chloroform fraction. In conclusion, E. mattosii presented antimycoplasmic activity related, at least in part, to the presence of pinostrobin.

scholarly journals PHYTOCHEMICAL INVESTIGATION OF CHENOPODIUM MURALE (FAMILY: CHENOPODIACEAE) CULTIVATED IN IRAQ, ISOLATION AND IDENTIFICATION OF SCOPOLETIN AND GALLIC ACID

2017 ◽  
Vol 10 (11) ◽  
pp. 70
Author(s):  
Omer H Ahmed ◽  
Maha N Hamad ◽  
Noor S Jaafar
Keyword(s):  
Ethyl Acetate ◽  
Gallic Acid ◽  
Phenolic Acid ◽  
High Performance ◽  
Chemical Constituents ◽  
Ethyl Acetate Fraction ◽  
Absolute Methanol ◽  
Isolation And Identification ◽  
Chenopodium Murale ◽  
Phytochemical Investigation

  Objective: The aim of our study was to investigate chemical constituents of leaves of Chenopodium murale since no phytochemical investigation had been done previously in Iraq.Methods: Leaves of C. murale were macerated in absolute methanol for 2 days and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate fraction was analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and coumarins contents. Coumarin derivative and phenolic acid were isolated from this fraction and identified by gas chromatography/mass spectrometry, infrared, ultraviolet, HPLC, and HPTLC.Results: The different chromatographic and spectroscopic results revealed the presence of gallic acid and coumarin.Conclusion: The results of the current study showed the presence of scopoletin and gallic acid in the ethyl acetate fraction of C. murale.

scholarly journals Phenolic Constituents of Platanus Orientalis L. Leaves

2008 ◽  
Vol 3 (2) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Taha S. El-Alfy ◽  
Hamida M.A. El-Gohary ◽  
Nadia M. Sokkar ◽  
Amani A. Sleem ◽  
Dalia A. Al-Mahdy
Keyword(s):  
Ethyl Acetate ◽  
Phenolic Acid ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Flavonoid Glycoside ◽  
Cytotoxic Activities ◽  
Flavonoid Aglycones ◽  
Phenolic Constituents ◽  
Platanus Orientalis ◽  
Ethyl Acetate Extracts

A new flavonoid glycoside, 5,7,4′-trihydroxy-3,6-dimethoxyflavone-3′- O-β-D-xylopyranoside (axillarin-3′- O-β-D-xylopyranoside), was isolated from the ethyl acetate fraction of the ethanolic extract of Platanus orientalis L. leaves, along with nine known flavonoid aglycones and glycosides, a phenolic acid and a phenolic acid derivative. Their structures were established on the basis of detailed spectral analysis. The total ethanolic, aqueous and ethyl acetate extracts of the leaves were tested for antihepatotoxic, antioxidant and cytotoxic activities. The extracts were found to be effective in protection against CCl4 liver injury by significantly lowering alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels. The extracts also significantly restored depleted levels of glutathione, indicating a strong antioxidant activity, while they showed variable cytotoxic activity.

HPLC-ESI-MS/MS phenolic profile of ‟Nanicão Corupá” (Musa acuminata)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Amanda Rodrigues Borges ◽  
Ana Paula Jungston Capistrano ◽  
Camila Saatkamp ◽  
Luisana Lusia Silveira Utzig ◽  
Bruna Gonçalves Lopes ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Musa Acuminata ◽  
Industrial Property ◽  
Esi Ms ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Pre Treatment ◽  
First Time

Abstract “Nanicão Corupá” (Musa acuminata) comes from Southern Brazil. The tropical climate in the region provides unique characteristics, including a sweeter flavor. This difference resulted in a Geographical Indication Recognition and Designation of Origin, recognized by the National Institute of Industrial Property (INPI) in Brazil. Considering that “Nanicão Corupá” has some peculiarities related to the climate and there are no studies evaluating this banana cultivars, the purpose of the present study was to investigate the qualitative and quantitative phenolic composition of the aerial parts of “Nanicão Corupá” by HPLC-ESI-MS/MS in comparison to 46 commercial standards of phenolic compounds. Aerial parts (flower, leaves, fruit and stem) of “Nanicão Corupá” were collected and macerated in methanolic extracts, which were partitioned with solvents of different polarities (dichloromethane and ethyl acetate). The HPLC-ESI-MS/MS analysis was performed using the sample pre-treatment, chromatographic and mass spectrometer parameters. Results demonstrated that a total of 11 phenolic compounds were identified in the analyzed samples. The majority of compounds was identified in the ethyl acetate fraction (BFEF) of banana flowers: rutin (36.06 ± 0.23) and isoquercetin (28.83 ± 5). The compounds isoquercetin, naringerin and myricitrin were identified for the first time in the Musa genus.

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