scholarly journals Antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers towards Streptococcus sanguis ATCC 10566

2018 ◽  
Vol 30 (3) ◽  
pp. 190
Author(s):  
Fajar Fatriadi ◽  
Dikdik Kurnia ◽  
Mieke Hemiawati Satari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Chlorhexidine Gluconate ◽  
Streptococcus Sanguis ◽  
Microdilution Method ◽  
Methanolic Extracts ◽  
Natural Remedies

Introduction: Streptococcus sanguis is an initial cause of dental plaque formation which is the initial cause of caries. One of the preventive treatments can be done by using the mouthwash containing antibacterial substances. Along time, natural remedies are proven to be having more antibacterial properties. Ant-plant (Myrmecodia pendens Merr. & Perry) tubers are types of epiphytic plant that grows in many parts of Papua, with many health benefits, and are known to contain flavonoids, tannins, and tocopherols. This study was aimed to determine the antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers on the growth of Streptococcus sanguis ATCC 10566. Methods: The ant-plant tubers were extracted with soxhletation method using the methanol solvent. The fractionation was then performed using ethyl acetate to obtain the ethyl acetate fraction. Result: The phytochemical test showed that the ethyl acetate fraction of the ant-plant contained phenolics, tannins, flavonoids, and terpenoids. The bacterial test in this study was using the microdilution method using the ELISA Reader by measuring the Minimum Inhibitory Concentration (MIC) of ethyl acetate fraction of ant-plant tubers towards Streptococcus sanguis with positive control was using the chlorhexidine gluconate as the golden standard medication. The ethyl acetate fraction of ant-plant tuber had an antibacterial effect towards Streptococcus sanguis in the MIC of 31.25 ppm, while chlorhexidine gluconate was 0.49 ppm. Conclusion: The ethyl acetate fraction of ant-plant had a lower antibacterial activity compared to chlorhexidine gluconate on the growth of Streptococcus sanguis ATCC 10566.Keywords: Ant-plant tubers, Myrmecodia pendens Merr. & Perry, antibacterial activity, Streptococcus sanguis ATCC 10566.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽  
2012 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Muhtadi , MSi. ◽  
Ria Ambarwati ◽  
Ratna Yuliani
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

Anthraquinones Derivatives and Its Antibacterial Properties from Paederia foetida Stems

2019 ◽  
Vol 10 ◽  
Author(s):  
Aryandi Ramadhan ◽  
Agustin Krisna Wardani ◽  
Chi-I Chang
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Positive Control ◽  
Bioactive Constituents ◽  
Chemical Structures ◽  
Normal Phase Hplc ◽  
Paederia Foetida ◽  
Agar Well Diffusion Assay

Background: Paederia foetida (Rubiaceae) locally known as Chinese fever vine is a prominent plant species in the east and south Asia. The extract of paederia foetida Linn. has been used for the treatment of gastric infections or other digestive disorders in Chinese Traditional Medicine. Objective: The main aim of the study was to isolate bioactive constituents of P. foetida stem through a bio-guided assay, then evaluate their antibacterial activity and compare them with standard agents. Materials and Methods: The stems of P. foetida were extracted by methanol and successively partitioned with ethyl acetate and n-butanol. The ethyl acetate layer further fractionated using column chromatography and normal phase HPLC. The chemical structures of the isolated compounds were elucidated through comparison of the 1H and 13C NMR and MS spectral data with the literature. The antibacterial activity of P. foetida stem was evaluated using agar well diffusion assay and resazurin based micro-dilution technique. Results: Ten compounds were isolated from the Chinese fever vine stem including four anthraquinones, morindaparvin A (1), 1,3-dihydroxy-2-methoxyanthraquinone (2), digiferrol (3), and alizarin (4); two steroids, β-sitosterol (5), and stigmastan-3-one (6); two coumarins, scopoletin (7) and fraxidin (8); two aromatics, ferulic acid (9) and vanillic acid (10). The four anthraquinones 1-4 were isolated for the first time from Chinese fever vine stem. Compound 2 and 3 significantly inhibited staphylococcus aureus with MIC values 18.75 and 9.37 μg/mL respectively, and streptomycin (1.8 μg/mL) was used as a positive control. Conclusion: Compound 2 and 3 can be considered as a prospective candidate for the treatment of staphylococcal bacterial infections in both human and animals.

scholarly journals ANTIBACTERIAL EFFECTS OF SARANG SEMUT (MYRMECODIA PENDANS) FRACTIONS USING THREE DIFFERENT SOLVENTS TOWARD ENTEROCOCCUS FAECALIS CPS2

2020 ◽  
pp. 271-275
Author(s):  
CIPTADHI TRI OKA BINARTHA ◽  
ENDANG SUPRASTIWI ◽  
DIDIK KURNIA ◽  
ANGGRAINI MARGONO ◽  
DEWA AYU NYOMAN PUTRI ARTININGSIH
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Enterococcus Faecalis ◽  
Inhibition Zone ◽  
Ethyl Acetate Fraction ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Diffusion Method ◽  
Water Fractions ◽  
Layer Chromatography

Objective: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane),semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2.Methods: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groupsof solvent to obtain the n-hexane, ethyl acetate, and water fractions. The active compound from the best fraction group was identified using aphytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group was divided into five differentconcentrations, i.e. 20%, 40%, 60%, 80%, and 100% and was assessed against E. faecalis cps2 with an agar diffusion method. Chlorhexidine 2% wasused as a positive control. The width of the inhibition zone was calculated.Results: The ethyl acetate group had the biggest inhibition zone of 21 mm in diameter compared to n-hexane and water, which was 15 mm and19 mm in diameter, respectively. The MIC value of the fraction with a 20% concentration of ethyl acetate was significantly different (P < 0.05) from then-hexane and water solvents in inhibiting the growth of E. faecalis cps2.Conclusion: The ethyl acetate fraction of sarang semut had a greater inhibitory effect on E. faecalis cps2. In addition, the antibacterial activity of thefraction increased with an increase in concentration.

scholarly journals ANALISIS KORELASI ANTARA FLAVONOID TOTAL DENGAN AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI DAUN GEDI HIJAU (Abelmoschus manihot L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 591
Author(s):  
Josua A.T. Suoth ◽  
Sri Sudewi ◽  
Defny S. Wewengkang
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content

One chemichal component that is often reported to have pharmacological activity against treatment or prevention of disease is flavonoids which can be found in gedi hijau leaves. Staphylococcus aureus is the main cause of infection in humans which can cause various severe infections. This study aims to determine the total flavonoids content, minimum inhibitory concentration (MIC). To determine the correlation between total flavonoids and antibacterial activity of extract and fractions (Abelmoschus manihot L.), The method of this study was done by obtaining the results of total flavonoid content, testing the antibacterial activity by dilution using a positive control of ciprofloxacin. The results showed the highest total flavonoid content in ethyl acetate fraction, and the best antibacterial activity in ethyl acetate fraction. Correlation between total flavonoid content from extract and fraction (Abelmoschus manihot L.) with antibacterial activity against Staphylococcus aureus bacteria on ethyl acetate fraction, n-hexane fraction, and ethanol extract at a concentration of 1000 µg / mL had good values with kneeling significance values according to 0.012 (p <0.05), 0.034 (p <0.05), and 0.023 (p <0.05) with total flavonoid content affecting how much antibacterial activity is 40% for ethyl acetate fraction, 35% fraction n -heksane and 33% ethanol extract. Keywords         : Antibacterial, Total Flavonoids, Staphylococcus aureus, Abelmoschus manihot L. ABSTRAK Salah satu komponen kimia yang sering dilaporkan memiliki aktivitas farmakologi terhadap pengobatan atau pencegahan penyakit adalah flavonoid yang dapat ditemukan pada daun gedi hijau. Staphylococcus aureus merupakan kuman penyebab utama infeksi pada manusia yang dapat menyebabkan berbagai infeksi berat. Penelitian ini bertujuan menentukan kandungan flavonoid total, aktivitas antibakteri dan korelasi antara flavonoid total dengan aktivitas antibakteri dari ekstrak dan fraksi (Abelmoschus manihot L.). Metode penelitian ini dilakukan dengan mendapatkan hasil kandungan flavonoid total, uji aktivitas antibakteri dengan cara dilusi menggunakan kontrol positif ciprofloxacin. Hasil penelitian didapatkan kandungan flavonoid total tertinggi pada fraksi etil asetat, dan aktivitas antibakteri paling bagus pada fraksi etil asetat. Korelasi antara kandungan flavonoid total dari ekstrak dan fraksi (Abelmoschus manihot L.) dengan aktivitas antibakteri terhadap bakteri Staphylococcus aureus pada fraksi etil asetat, fraksi n-heksane, dan ekstrak etanol pada konsentrasi 1000 µg/mL memiliki nilai yang baik baik dengan nilai signifikansi bertutut-turut 0,012 (p <0,05), 0,034  (p <0,05), dan 0,023 (p <0,05) dengan kandungan flavonoid total mempengaruhi seberapa besar aktivitas antibakteri yaitu 40% untuk fraksi etil asetat, 35% fraksi n-heksane dan 33% ekstrak etanol.Kata kunci          : Antibakteri, Flavonoid Total, Staphylococcus aureus, Abelmoschus manihot L.

scholarly journals Profil fitokimia dan aktivitas antibakteri fraksi etil asetat akar jarak pagar (Jatropha curcas Linn.)

2021 ◽  
Vol 2 (2) ◽  
pp. 73-79
Author(s):  
Sudarman Rahman ◽  
Stevin Carolius Angga ◽  
Erwin Prasetya Toepak ◽  
Muhammad Takdir Bachtiar
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Jatropha Curcas ◽  
Bacterial Infections ◽  
Folk Medicine ◽  
Antibacterial Properties ◽  
Ethyl Acetate Fraction ◽  
Inhibitory Zone ◽  
Chronic Infections ◽  
Inhibitory Potency

Bacterial infections are a major cause of chronic infections and mortality that continues to threaten public health worldwide. Various medicinal plants used in folk medicine have demonstrated wound healing and antibacterial properties. The aims of the study are investigation phytochemical and antibacterial activity of ethyl acetate fraction from jarak pagar roots (Jatropha curcas Linn.). The roots were treated with maceration method using ethanol 70% and subsequently fractioned to ethyl acetate fraction. The fractions were used for phytochemical screening and antibacterial assay using Kirby and Bauer agar difussion method. The results showed that ethyl acetate fraction jarak pagar roots contained alkaloid, flavonoids, saponins, steroids, poliphenol dan tannins. The antibacterial activity of jarak pagar roots againts Staphylococcus aureus and Escherichia coli shows that the activity of this fraction with concentration 8 mg/mL has strong inhibitory potency against the growth S. aureus with inhibitory zone is 14,3 mm and has moderate inhibitory potency against E. coli with inhibitory zone is 9 mm. The increase concentrations of ethyl asetate fraction jarak pagar roots shows high inhibition diameter of bacterial growth.  

scholarly journals Antibacterial Activity of Scopoletin from Stem Bark of Aleurites moluccana Against Salmonella typhi

2020 ◽  
Vol 5 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Wisnu Cahyo Prabowo ◽  
Risna Agustina
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
East Kalimantan ◽  
Antibacterial Screening ◽  
Microdilution Method ◽  
The People ◽  
Aleurites Moluccana

Background: Based on a previous survey of ethnopharmaceutical studies (local culture), it was known that the use of decoction of candlenut stem bark (Aleurites Moluccana) was used as typhoid medicine in the treatment tradition of the people of East Kalimantan. In antibacterial screening by diffusion test, methanol extract of stem bark A.moluccana showed excellent growth inhibition of Salmonella thypimurium. Working method: Simplicia of A.moluccana stem bark was extracted by maceration using methanol. Maserate was extracted liquid-liquid using n-hexane and ethyl acetate solvents. The hexane and ethyl acetate fraction was monitored for active spots with TLC bioautography. The active compound is separated using vacuum liquid chromatography and radial chromatography. Results: The fraction J of KCV and J2 subfraction results from radial chromatography provides antibacterial activity of S. thpimurium. From the J2 subfraction obtained pure isolates in the form of yellowish needle crystals. The isolate was tested for antibacterial S.thypimurium using the microdilution method with a value of MIC is 250 µg/ml. Based on spectroscopic data and comparing the published spectra of the compound, the elucidation of the isolate is Scopoletin (7-hydroxy-6-methoxycoumarin).

scholarly journals ANTI-BACTERIAL ACTIVITY TEST OF ETHANOL EXTRACTS AND ETHLACETATE FRACTION FROM THE EXTRACT OF JATROPHA CURCAS L. LEAVES AGAINST STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 5 (1) ◽  
pp. 31
Author(s):  
Sekar Wulandari
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Diffusion Method ◽  
Resistance Response ◽  
Activity Test ◽  
Significant Difference

Background: Staphylococcus aureus is a gram-positive bacteria that can cause infection. One of the plants that has antibacterial activity is jatropha leaves which contain flavonoids, saponins, tannins, alkaloids, steroids and polyphenols. Purpose: To determine the antibacterial activity of ethanol extract and ethyl acetate fraction with concentrations of 30%, 60% and 100% against Staphylococcus aureus bacteria. Method: The method for extracting jatropha leaves is maceration with 96% ethanol solvent and the fractionation method, namely liquid-liquid fractionation with ethyl acetate solvent. Antibacterial activity test was carried out in vitro with the disc diffusion method and compared the mean zone of inhibition of each treatment with a positive control (gentamicin 10 μg). Result: The results showed that the ethanol extract and ethyl acetate fraction of jatropha leaves had a strong resistance response, while the positive control gave a very strong inhibitory response to the growth of S. aureus bacteria. Based on the one way ANOVA test, ethanol extract and ethyl acetate fraction showed a significant difference from each treatment with a significant value (P=<0.05). Conclusion: The ethanol extract of Jatropha leaves can inhibit the growth of S. aureus bacteria at a concentration of 100% (18.28 ± 0.50 mm), 100% concentration of ethyl acetate fraction (15.10 ± 0.12 mm). The ethanol extract provided the best inhibition power, namely 18.28 ± 0.50 mm and a positive control 21.82 ± 0.092 mm.

scholarly journals Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Resmi Mustarichie ◽  
Sulistiyaningsih Sulistyaningsih ◽  
Dudi Runadi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Clinical Isolates ◽  
Active Fraction ◽  
Cassava Leaves ◽  
Ethanol Extracts

This study is aimed at determining antibacterial activity from ethanol extracts and the most active fraction of cassava leaves against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes. Research carried out by the experimental method involved determination of plants, extraction with maceration method, fractionation with liquid-liquid extraction, antibacterial activity testing of extracts and fractions by agar diffusion method, determination of most active fraction from the extract, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) testing of most active fraction by microdilution method. The results showed that ethanol extracts of cassava leaves had antibacterial activity against both bacteria with the most active fraction indicated by ethyl acetate. MIC values of ethyl acetate fraction against S. epidermidis were in the concentration range of 2.5%–5.0% (w/v) and against P. acnes were in the concentration range of 1.25%–2.5% (w/v). The MBC value of ethyl acetate fraction against S. epidermidis was at a concentration of 5% (w/v), while P. acnes was at a concentration of 2.5% (w/v). From the results of this study, it can be concluded that the ethanol extract of cassava leaves (Manihot esculenta Crantz) has antibacterial activity against clinical isolates of Staphylococcus epidermidis as well as on Propionibacterium acnes. The fraction with the best activity from the ethanol extract of cassava leaves to the two test bacteria was shown by ethyl acetate fraction. It is suggested that cassava leaves are possible to be developed into standardized antiacne herbal.

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

scholarly journals Uji Aktivitas Antibakteri dari Ekstrak dan Fraksi Daging Buah Mangga Kasturi (Mangifera Casturi) terhadap Bakteri Pseudomonas Aeruginosa dan Bacillus Cereus

2021 ◽  
Vol 49 (2) ◽  
pp. 113-122
Author(s):  
Meliana Meliana ◽  
Sogandi Sogandi ◽  
Ekajayanti Kining
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Mangifera Indica ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry

Kasturi (Mangifera casturi) is a typical plant of South Kalimantan which has only been used for consumption because of its distinctive taste and aroma. Mangifera indica, which is in the same genus as Mangifera casturi, shows antioxidant, antibacterial, antifungal, and antidiarrheal activity, so it is suspected that this plant has the same activity. Crude methanol extract from the meat of Casturi mango was fractionated using n-hexane, ethyl acetate, and methanol-water. Testing for antibacterial activity and minimum inhibitory concentration (MIC) were carried out using the disk diffusion method (Kirby-Bauer). The positive control used was Chloramphenicol because it has an antibacterial range that is rather broad and the negative control used was 10% DMSO. The fraction that had the greatest antibacterial activity was the ethyl acetate fraction; with the formation of a clear zone of 7.98mm against Pseudomonas aeruginosa and 7.52mm against Bacillus cereus, with a minimum inhibitory concentration of 2mg/mL. The next test was a cell leakage analysis which revealed an increase in absorbance at MIC 1 and MIC 2. The Gas Chromatography – Mass Spectrometry (GC-MS) results from the ethyl acetate fraction revealed the presence of phenolic, flavonoid, and glycoide content, with the highest compound concentration of 5-Hydroxymethylfurfural. Keywords: Antibacterial, Extract, Ethyl acetate, fraction, Mangifera casturi Abstrak Kasturi (Mangifera casturi) merupakan tumbuhan khas Kalimantan Selatan yang selama ini hanya dimanfaatkan untuk dikonsumsi karena rasa dan aromanya yang khas. Mangifera indica yang masih satu genus dengan Mangifera casturi menunjukkan aktivitas antioksidan, antibakteri, antijamur, dan antidiare sehingga diduga tanaman ini memiliki aktivitas yang sama. Ekstrak kasar metanol daging buah mangga kasturi difraksinasi dengan menggunakan n-heksan, etil asetat, dan metanol-air. Pengujian aktivitas antibakteri dan kadar hambat minimum dilakukan dengan menggunakan metode difusi cakram (Kirby- Bauer). Kontrol positif yang digunakan adalah Chloramfenikol karena memiliki spektrum antibakteri yang cukup luas dan kontrol negatif yang digunakan adalah DMSO 10%. Fraksi yang memiliki aktivitas antibakteri terbesar pada penelitian ini adalah fraksi etil asetat dengan terbentuknya zona bening sebesar 7,98mm terhadap Pseudomonas aeruginosa dan 7,52mm terhadap Bacillus cereus dengan kadar hambat minimum 2mg/mL. Uji selanjutnya adalah uji kebocoran sel yang menyatakan adanya peningkatan absorbansi pada KHM 1 dan KHM 2. Hasil pemeriksaan Gas Chromatography – Mass Spectrometry (GCMS) dari fraksi etil asetat menyatakan adanya kandungan fenolik, flavonoid, dan glikosida dengan senyawa tertinggi yaitu 5-Hydroxymethylfurfural. Kata kunci: Antibakteri, Ekstrak, Etil asetat, Fraksi, Mangifera castur

Sign in / Sign up

Export Citation Format

Share Document