scholarly journals Production of Single Cells from Plant Materials and Softening by an Enzyme Infusion Method Under Reduced Pressure After Freezing

2004 ◽  
Vol 51 (8) ◽  
pp. 395-400 ◽  
Author(s):  
Koji Sakamoto ◽  
Masako Ishihara ◽  
Kenya Shibata ◽  
Atsuhiko Inoue
Keyword(s):  
Single Cells ◽  
Reduced Pressure ◽  
Plant Materials ◽  
Infusion Method ◽  
Enzyme Infusion

scholarly journals Phytochemical Investigation of Corchorus olitorius L. Leaves Cultivated in Iraq and it’s In Vitro Antiviral Activity

2018 ◽  
pp. 115-122
Author(s):  
Hayder T. Hasan ◽  
Eman J. Kadhim
Keyword(s):  
Antiviral Activity ◽  
Measles Virus ◽  
Chemical Constituents ◽  
Basic Solution ◽  
Multiplicity Of Infection ◽  
Corchorus Olitorius ◽  
Reduced Pressure ◽  
Plant Materials ◽  
Soxhlet Apparatus ◽  
Aqueous Layer

The aim of our study was to investigate the antiviral activity of the Corchorus olitorius family Tiliaceae cultivated in Iraq against measles virus, and to demonstrate an overview about chemical constituents and pharmacological activity of Corchorus olitorius L. About150 gm Leaves of Corchorus. olitorius were defatted by maceration in hexane for 24 hrs. The defatted plant materials were subjected for extraction after filtration using Soxhlet apparatus, with aqueous methanol 85% as a solvent extraction for 24 hours, the extract was filtered, and the solvent was evaporated under reduced pressure using a rotary evaporator to get a dry extract of about 12 gm. About 4 gm from the residue was suspended in 100ml water, about 3-4ml of 5% sodium hydroxide was added to obtain a basic solution having PH 10 and partitioned with ethyl acetate (3x100ml), the aqueous layer collected and evaporated to dryness. MTT-cell viability assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) was conducted on 96-well plates (Falcon), Vero cells were seeded at 1× 104 cells/well to obtain a multiplicity of infection (MOI 10), and at 5 × 103 cells/well to obtain a multiplicity of infection (MOI 5). Different statistical result revealed a significant antiviral activity of the aqueous layer of Corchorus olitorius leaves against measles virus. The preliminary phytochemical tests showed the presence of phenols and flavonoids in the aqueous layer of Corchorus olitorius leaves. The antiviral activity of Corchorus olitorius leaves is mainly due to the phenolics and flavonoids that detected in the aqueous layer.

Decreased Hardness of Dietary Fiber-Rich Foods by the Enzyme-Infusion Method

2006 ◽  
Vol 70 (7) ◽  
pp. 1564-1570 ◽  
Author(s):  
Koji SAKAMOTO ◽  
Kenya SHIBATA ◽  
Masako ISHIHARA
Keyword(s):  
Dietary Fiber ◽  
Infusion Method ◽  
Enzyme Infusion

scholarly journals Experimental Data of Grinding Dried Fibrous Plant Materials

2021 ◽  
Vol 31 (4) ◽  
pp. 591-608
Author(s):  
Sergey V. Braginets ◽  
Oleg N. Bakhchevnikov ◽  
Aleksandr S. Alferov
Keyword(s):  
Energy Intensity ◽  
Fractional Composition ◽  
Raw Materials ◽  
Specific Power ◽  
Energy Capacity ◽  
Operating Characteristics ◽  
Optimal Range ◽  
Reduced Pressure ◽  
Plant Materials ◽  
Experimental Apparatus

Introduction. Developing a method for energy-efficient grinding of fibrous vegetable raw materials to avoid the clogging of grids remains an urgent task. The aim of the research is to study the process of grinding dried fibrous plant materials and to estimate the influence of the device operating characteristics on the quality of grinding and the process energy intensity. Materials and Methods. The experimental apparatus is a rotor grinder. Its working bodies are alternate knives and hammers. When a hammer is in motion, its triangle side creates the reduced pressure area. There was studied the influence of the linear velocities of knife motion and of feed of raw materials on fractional composition of the grinded materials, grinder productivity, and grinding specific energy capacity. Results. It is found that the change in the fractional composition of the grinded product occurs when the speed of the rotor knives increases. Optimal range of knife speed for producing the product of the required fractional composition is 55‒75 m/s. The increase in the speed leads to increasing productivity, but is accompanied by the growth of specific power intensity. If the rotor speed is constant, the increase of raw material feed increases the grinder productivity, but only up to a certain value. After that, the productivity decreases because of excessive filling of the working chamber with raw materials and clogging of the grates. For each value of the knife speed, there is an optimal feed that ensures the maximum productivity. High values of knife speed lead to significant energy intensity of the process and overgrinding of raw materials. Therefore, the optimal range of knife speed is 55‒65 m/s. Discussion and Conclusion. Effective grinding of raw materials is achieved through lower energy capacity of grinding process and absence of grate clogs resulted from separating particles from the surface of plants to be grinded.

scholarly journals PHYTOCHEMICAL INVESTIGATION AND PHARMACOLOGICAL ACTIVITY OF CORCHORUS OLITORIUS L. CULTIVATED IN IRAQ.

2018 ◽  
pp. 115-123
Author(s):  
Hayder Taher Hasen
Keyword(s):  
Antiviral Activity ◽  
Pharmacological Activity ◽  
Measles Virus ◽  
Basic Solution ◽  
Multiplicity Of Infection ◽  
Corchorus Olitorius ◽  
Reduced Pressure ◽  
Plant Materials ◽  
Soxhlet Apparatus ◽  
Aqueous Layer

Objective: The aim of our study was to investigate the antiviral activity of the Corchorus olitorius family tiliaceae cultivated in Iraq against measles virus, and to demonstrate an overview about chemical constituents and pharmacological activity of Corchorus olitorius L. Methods: Leaves of Corchorus. olitorius were defatted by maceration in hexane for 24 hrs. The defatted plant materials were subjected for extraction using Soxhlet apparatus, the aqueous methanol 85% as a solvent extraction for 24 hours, the extract was filtered, and the solvent was evaporated under reduced pressure using a rotary evaporator to get a dry extract of about 12 gm. The residue was suspended in 100ml water, about 3-4ml of 5% sodium hydroxide was added to obtain a basic solution having PH 10 and partitioned with ethyl acetate (3x100ml), the aqueous layer collected and evaporated to dryness. MTT-cell viability assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) was conducted as on 96-well plates (Falcon), Vero cells were seeded at 1× 104 cells/well to obtain a multiplicity of infection (MOI 10), and at 5 × 103 cells/well to obtain multiplicity of infection (MOI 5). Results: The different statistical result revealed a significant antiviral activity of the aqueous layer of  Corchorus olitorius leaves against measles virus. the preliminary phytochemical tests show the presence of phenols and flavonoids in the aqueous layer of  Corchorus olitorius leaves. Conclusion: The antiviral activity of Corchorus olitorius leaves is mainly due to the phenolics and flavonoids that detected in the aqueous layer.

scholarly journals AKTIVITAS INSEKTISIDA BAGIAN TUMBUHAN CALOPHYLLUM SOULTTRI BURM.F. (CLU IACEAE) TERHADAP LARVA LEPIDOPTERA

2004 ◽  
Vol 4 (1) ◽  
pp. 23-31
Author(s):  
Edy Syahputra ◽  
Syafrida Manuwoto ◽  
Latifah K Darusman ◽  
Dadang Dadang ◽  
Djoko Prijono
Keyword(s):  
Insecticidal Activity ◽  
Larval Mortality ◽  
Developmental Time ◽  
Fourth Instar ◽  
Bark Extract ◽  
Plant Materials ◽  
Feeding Method ◽  
Highly Active ◽  
Infusion Method ◽  
Probit Method

 The objective of this test was evaluate the insecticidal activity of ectract of some parts of Calophyllum soulattri (Clusiaceae) against larvae of three species of Lepidoptera, i. e Crocidolomia pavonana, Plutella xylostela, and Pieris sp.. Extraction of plant materials was done by infusion method using ethanol. The bioassays were conducted by leaf-feeding method. Second-instar larvae were fed extract-treated broccoli leaves of 48 hours, then they were presented with untreated leaves until the surviving larvae larvae reached the fourth-instar stage. The number of dead larvae was recorded daily an larval mortality date were analyzed by probit method. The result showed the gummy bark exudates and bark extract of old and young C. soulattri plants were highly active against C. pavonana. The abrk extact of old C. soulattri plant was also effective against P. xilostella and Pieris sp. The gummy exudates possessed strong insecticidal activity against C. pavonana larvae with LC50 of 0.04% and prolonged the developmental time from second to fourth instar of C. soulattri 2.03-7.25 days compared with control. The bark excudate gave positive respon to alkaloid flavonoid, and tannin test. Futher studies are needed to identify insecticidal compound in those active extracts.

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

Selective Staining of Acid Mucopolysaccharides By Ruthenium Red

1968 ◽  
Vol 26 ◽  
pp. 38-39
Author(s):  
J. H. Luft
Keyword(s):  
Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Osmium Tetroxide ◽  
Single Cells ◽  
Ruthenium Red ◽  
Collagen Fibers ◽  
Selective Staining ◽  
Pectic Substances ◽  
Solid Tissues

Ruthenium red is one of the few completely inorganic dyes used to stain tissues for light microscopy. This novelty is enhanced by ignorance regarding its staining mechanism. However, its continued usefulness in botany for demonstrating pectic substances attests to selectivity of some sort. Whether understood or not, histochemists continue to be grateful for small favors.Ruthenium red can also be used with the electron microscope. If single cells are exposed to ruthenium red solution, sufficient mass can be bound to produce observable density in the electron microscope. Generally, this effect is not useful with solid tissues because the contrast is wasted on the damaged cells at the block surface, with little dye diffusing more than 25-50 μ into the interior. Although these traces of ruthenium red which penetrate between and around cells are visible in the light microscope, they produce negligible contrast in the electron microscope. However, its presence can be amplified by a reaction with osmium tetroxide, probably catalytically, to be easily visible by EM. Now the density is clearly seen to be extracellular and closely associated with collagen fibers (Fig. 1).

Fluorescent potentiometric dyes for membrane-potential imaging

1994 ◽  
Vol 52 ◽  
pp. 166-167
Author(s):  
Leslie M. Loew
Keyword(s):  
Membrane Potential ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Optical Recording ◽  
Biological Processes ◽  
Major Focus ◽  
The Past ◽  
Excitable Systems ◽  
Major Application ◽  
The Impact

A major application of potentiometric dyes has been the multisite optical recording of electrical activity in excitable systems. After being championed by L.B. Cohen and his colleagues for the past 20 years, the impact of this technology is rapidly being felt and is spreading to an increasing number of neuroscience laboratories. A second class of experiments involves using dyes to image membrane potential distributions in single cells by digital imaging microscopy - a major focus of this lab. These studies usually do not require the temporal resolution of multisite optical recording, being primarily focussed on slow cell biological processes, and therefore can achieve much higher spatial resolution. We have developed 2 methods for quantitative imaging of membrane potential. One method uses dual wavelength imaging of membrane-staining dyes and the other uses quantitative 3D imaging of a fluorescent lipophilic cation; the dyes used in each case were synthesized for this purpose in this laboratory.

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