scholarly journals PHYTOCHEMICAL INVESTIGATION AND PHARMACOLOGICAL ACTIVITY OF CORCHORUS OLITORIUS L. CULTIVATED IN IRAQ.

2018 ◽  
pp. 115-123
Author(s):  
Hayder Taher Hasen
Keyword(s):  
Antiviral Activity ◽  
Pharmacological Activity ◽  
Measles Virus ◽  
Basic Solution ◽  
Multiplicity Of Infection ◽  
Corchorus Olitorius ◽  
Reduced Pressure ◽  
Plant Materials ◽  
Soxhlet Apparatus ◽  
Aqueous Layer

Objective: The aim of our study was to investigate the antiviral activity of the Corchorus olitorius family tiliaceae cultivated in Iraq against measles virus, and to demonstrate an overview about chemical constituents and pharmacological activity of Corchorus olitorius L. Methods: Leaves of Corchorus. olitorius were defatted by maceration in hexane for 24 hrs. The defatted plant materials were subjected for extraction using Soxhlet apparatus, the aqueous methanol 85% as a solvent extraction for 24 hours, the extract was filtered, and the solvent was evaporated under reduced pressure using a rotary evaporator to get a dry extract of about 12 gm. The residue was suspended in 100ml water, about 3-4ml of 5% sodium hydroxide was added to obtain a basic solution having PH 10 and partitioned with ethyl acetate (3x100ml), the aqueous layer collected and evaporated to dryness. MTT-cell viability assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) was conducted as on 96-well plates (Falcon), Vero cells were seeded at 1× 104 cells/well to obtain a multiplicity of infection (MOI 10), and at 5 × 103 cells/well to obtain multiplicity of infection (MOI 5). Results: The different statistical result revealed a significant antiviral activity of the aqueous layer of  Corchorus olitorius leaves against measles virus. the preliminary phytochemical tests show the presence of phenols and flavonoids in the aqueous layer of  Corchorus olitorius leaves. Conclusion: The antiviral activity of Corchorus olitorius leaves is mainly due to the phenolics and flavonoids that detected in the aqueous layer.

scholarly journals Phytochemical Investigation of Corchorus olitorius L. Leaves Cultivated in Iraq and it’s In Vitro Antiviral Activity

2018 ◽  
pp. 115-122
Author(s):  
Hayder T. Hasan ◽  
Eman J. Kadhim
Keyword(s):  
Antiviral Activity ◽  
Measles Virus ◽  
Chemical Constituents ◽  
Basic Solution ◽  
Multiplicity Of Infection ◽  
Corchorus Olitorius ◽  
Reduced Pressure ◽  
Plant Materials ◽  
Soxhlet Apparatus ◽  
Aqueous Layer

The aim of our study was to investigate the antiviral activity of the Corchorus olitorius family Tiliaceae cultivated in Iraq against measles virus, and to demonstrate an overview about chemical constituents and pharmacological activity of Corchorus olitorius L. About150 gm Leaves of Corchorus. olitorius were defatted by maceration in hexane for 24 hrs. The defatted plant materials were subjected for extraction after filtration using Soxhlet apparatus, with aqueous methanol 85% as a solvent extraction for 24 hours, the extract was filtered, and the solvent was evaporated under reduced pressure using a rotary evaporator to get a dry extract of about 12 gm. About 4 gm from the residue was suspended in 100ml water, about 3-4ml of 5% sodium hydroxide was added to obtain a basic solution having PH 10 and partitioned with ethyl acetate (3x100ml), the aqueous layer collected and evaporated to dryness. MTT-cell viability assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) was conducted on 96-well plates (Falcon), Vero cells were seeded at 1× 104 cells/well to obtain a multiplicity of infection (MOI 10), and at 5 × 103 cells/well to obtain a multiplicity of infection (MOI 5). Different statistical result revealed a significant antiviral activity of the aqueous layer of Corchorus olitorius leaves against measles virus. The preliminary phytochemical tests showed the presence of phenols and flavonoids in the aqueous layer of Corchorus olitorius leaves. The antiviral activity of Corchorus olitorius leaves is mainly due to the phenolics and flavonoids that detected in the aqueous layer.

Functional Group Manipulations

2008 ◽  
Author(s):  
Jie Jack Li ◽  
Chris Limberakis ◽  
Derek A. Pflum
Keyword(s):  
Column Chromatography ◽  
Room Temperature ◽  
Functional Group ◽  
Lithium Bromide ◽  
Methyl Methanesulfonate ◽  
Organic Layer ◽  
Reduced Pressure ◽  
Organic Extracts ◽  
Aqueous Layer ◽  
G 34

CBr4–Ph3P is very straightforward and widely used. Workup and purification can be messy at times because of the by-product, Ph3PO. To a mixture of the alcohol (0.800 g, 3.36 mmol) and carbon tetrabromide (1.337 g, 4.03 mmol) in CH2Cl2 at 0 ºC was added a solution of PPh3 (1.319 g, 5.03 mmol) in CH2Cl2 (3 mL). The reaction mixture was stirred at room temperature for 1 h, concentrated under reduced pressure, and purified by column chromatography to afford the bromide (0.941 g, 93% yield). Reference: Hu, T.-S.; Yu, Q.; Wu, Y.-L.; Wu, Y. J. Org. Chem. 2001, 66, 853–861. A two-step sequence consisting of mesylate formation followed by treatment with LiBr can also be used. This procedure involves two steps, but workup and purification are very straightforward. The bromide can be carried out to the next step without further purification in many cases. To a solution of 5-hydroxymethyl-1-methylcyclopentene (3.8 g, 34 mmol) in CH2 Cl2 (50 mL) at 0 ºC was added triethylamine (5.2 mL, 37 mmol) followed by methanesulfonyl chloride (2.9 mL, 37 mmol). The mixture was stirred at 0 ºC for 5 h and then water was added. The organic layer was separated and the aqueous layer was extracted with ether. The combined organic extracts were dried over MgSO4 and the solvent was removed under reduced pressure to give 6.4 g (98%) of (2-methylcyclopent-2- enyl)methyl methanesulfonate, which was used in the next step without further purification. A solution containing the mesylate (6.4 g, 34 mmol) in acetone (70 mL) was treated with lithium bromide (8.89 g, 102 mmol). The mixture was heated at reflux for 6 h, cooled to room temperature, diluted with water, extracted with ether, and the combined ethereal extracts were dried over MgSO4. Removal of the solvent under reduced pressure gave 4.6 g (78%) of 5-bromomethyl-1-methylcyclopentene, which was used in the next step without further purification.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF CORDIA OBLIQUA AGAINST PARACETAMOL-INDUCED LIVER TOXICITY ON WISTAR RATS

2019 ◽  
pp. 149-152
Author(s):  
SIVAKRISHNAN SIVAGNANAM ◽  
PRADEEPRAJ DEVARASU
Keyword(s):  
Wistar Rats ◽  
Liver Toxicity ◽  
Histological Study ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Plant Materials ◽  
Percolation Method ◽  
Soxhlet Apparatus ◽  
Serum Glutamate Pyruvate Transaminase

Objective: The ethanolic extract of Cordia obliqua (EECO) Willd. leaves was evaluated for hepatoprotective activity in Wistar rats by inducing hepatic damage with paracetamol. Methods: The leaves of C. obliqua were dried under shade, segregated, pulverized by a mechanical grinder and passed through a 40 mesh sieve. The powdered plant materials were stored in an air-tight container. The above-powdered materials were successively extracted with ethanol by hot continuous percolation method in Soxhlet apparatus for 24 h. The extract was concentrated using a rotary evaporator and subjected to freeze drying in a lyophilizer till dry powder was obtained. Results: Treatment with EECO at 200 and 400 mg/kg showed significant decrease in serum glutamate-pyruvate transaminase, serum glutamic oxaloacetic transaminase, alkaline phosphatase, and total bilirubin levels and a significant elevation in the total protein, albumin, and globulin levels in serum when compared with paracetamol treated rats. In histological study of liver, the hepatocytes show moderate cytoplasm and moderately enlarged pleomorphic and hyperchromatic nuclei. The portal triads show mild periportal inflammation composed of lymphocytes, and central veins are normal. Conclusion: On the basis of results, we can conclude that the EECO leaves showed potential hepatoprotective activity in rats.

scholarly journals Antiviral Activity of Favipiravir (T-705) against a Broad Range of ParamyxovirusesIn Vitroand against Human Metapneumovirus in Hamsters

2016 ◽  
Vol 60 (8) ◽  
pp. 4620-4629 ◽  
Author(s):  
D. Jochmans ◽  
S. van Nieuwkoop ◽  
S. L. Smits ◽  
J. Neyts ◽  
R. A. M. Fouchier ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Measles Virus ◽  
Rna Virus ◽  
Antiviral Drug ◽  
Specific Effect ◽  
Human Metapneumovirus ◽  
Drug Candidate ◽  
Emerging Viruses ◽  
Syncytial Virus

ABSTRACTThe clinical impact of infections with respiratory viruses belonging to the familyParamyxoviridaeargues for the development of antiviral therapies with broad-spectrum activity. Favipiravir (T-705) has demonstrated potent antiviral activity against multiple RNA virus families and is presently in clinical evaluation for the treatment of influenza. Here we demonstratein vitroactivity of T-705 against the paramyxoviruses human metapneumovirus (HMPV), respiratory syncytial virus, human parainfluenza virus, measles virus, Newcastle disease virus, and avian metapneumovirus. In addition, we demonstrate activity against HMPV in hamsters. T-705 treatment inhibited replication of all paramyxoviruses testedin vitro, with 90% effective concentration (EC90) values of 8 to 40 μM. Treatment of HMPV-challenged hamsters with T-705 at 200 mg/kg of body weight/day resulted in 100% protection from infection of the lungs. In all treated and challenged animals, viral RNA remained detectable in the respiratory tract. The observation that T-705 treatment had a significant effect on infectious viral titers, with a limited effect on viral genome titers, is in agreement with its proposed mode of action of viral mutagenesis. However, next-generation sequencing of viral genomes isolated from treated and challenged hamsters did not reveal (hyper)mutation. Polymerase activity assays revealed a specific effect of T-705 on the activity of the HMPV polymerase. With the reported antiviral activity of T-705 against a broad range of RNA virus families, this small molecule is a promising broad-range antiviral drug candidate for limiting the viral burden of paramyxoviruses and for evaluation for treatment of infections with (re)emerging viruses, such as the henipaviruses.

scholarly journals Interferon as a mediator of human lymphocyte suppression.

1980 ◽  
Vol 151 (3) ◽  
pp. 637-650 ◽  
Author(s):  
A S Kadish ◽  
F A Tansey ◽  
G S Yu ◽  
A T Doyle ◽  
B R Bloom
Keyword(s):  
Antiviral Activity ◽  
Cell Line ◽  
Measles Virus ◽  
Cell Activity ◽  
Suppressor Cells ◽  
Human Leukocyte ◽  
Suppressor Cell ◽  
Con A ◽  
Blood Leukocytes ◽  
Suppressor Activity

Evidence is presented that interferon (IF) is a major mediator of the human concanavalin A (Con A) suppressor cell. The suppressive effects of Con A-activated lymphocytes on the mitogen responses of normal responder cells were largely abrogated by addition of anti-human leukocyte IF serum. Similar suppressor activity was generated by coculture of peripheral blood leukocytes (PBL) with a melanoma cell line (MeWo) and a HeLa cell line persistently infected with measles virus that induced the production of IF by lymphocytes. A human mammary carcinoma line (MCF-7) and two bladder carcinoma lines (T24 and TCCSUP) failed to induce IF or suppression. Addition of anti-human leukocyte IF serum to suppressor cells and supernates from tumor cell-lymphocyte cocultures largely abolished suppression and neutralized the antiviral activity of such supernates. Exposure of PBL from purified protein derivative (PPD)-positive donors to PPD caused the production of suppressor activity and IF. PBL from PPD-negative donors failed to produce significant amounts of IF or to suppress on exposure to PPD. Supernates from PBL treated with virus (Newcastle disease virus [NDV]) contained IF and suppressed the mitogen responses of responder PBL. Both the suppressive and the antiviral activities of this material were eliminated after treatment with anti-IF serum. To ascertain whether antiviral and suppressive activities were mediated by the same types of IF, supernates from PBL cultured with Con A, PPD, NDV, and tumor cells were treated with anti-IF serum or acid pH. In all cases antiviral activity was neutralized in parallel with abrogation of suppressor activity. These results provide strong evidence for the role of IF as a mediator of human suppressor cell activity.

scholarly journals Phytochemical Investigation of Aerial Parts of Iraqi Cardaria draba

2020 ◽  
Vol 29 (2) ◽  
pp. 27-36
Author(s):  
Alaa M. Abd ◽  
Enas J. Kadhim
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acid ◽  
High Performance ◽  
Chemical Constituents ◽  
Aqueous Methanol ◽  
Plant Materials ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Soxhlet Apparatus ◽  
Layer Chromatography

 The aim of this study was to study chemical constituents of aerial parts of Cardaria draba since no phytochemical investigation had been studied before in Iraq. Aerial parts of Cardaria draba were defatted by maceration in hexane for 72 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous Methanol 90% as a solvent extraction for 18 h, and fractionated with petroleum ether- chloroform (CHCl3)- ethylacetate- and n-butanol respectivly. The ethyl acetate, n-butanol, and n-butanol after hydrolysis fractions were investigated by high performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) for its phenolic acid and flavonoid contents. Flavonoids and phenolic acid derivative were isolated from the ethylacetate of leaf fraction and n-butanol after hydrolysis fraction of the aerial parts and identified by TLC, FTIR and HPLC. A various chromatographic and spectroscopic results shown the presence of luteolin, chlorogenic acid, caffeic acid, and resorcinol in aerial parts of C. draba.                                                                                                                              

scholarly journals Phenol-rich alternatives for Rosa x damascena Mill. Efficient phytochemical profiling using different extraction methods and colorimetric assays

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zuzanna Piotrowicz ◽  
Łukasz Tabisz ◽  
Marta Waligórska ◽  
Radosław Pankiewicz ◽  
Bogusława Łęska
Keyword(s):  
Plant Extracts ◽  
Crop Yields ◽  
Radical Scavenging ◽  
In Vitro Assays ◽  
Plant Materials ◽  
Soxhlet Apparatus ◽  
Colorimetric Assays ◽  
Damask Rose

AbstractDamask rose is a well-established, abundant source of phytochemicals, as well as economically important essential oil—however, its cultivation is demanding and costly. In this paper, extracts from four raw plant materials—Salvia officinalis, Sambucus nigra, Matricaria chamomilla, Calendula officinalis, known to be rich in phenolic compounds, but also far easier to cultivate—were directly compared to those obtained from Rosa × damascena Mill. By combining diverse extraction methodologies (in a Soxhlet apparatus, ultrawave-assisted and microwave-assisted, using supercritical CO2) and complementary in vitro assays (radical scavenging, iron reducing, Folin–Ciocalteau and Al3+ complexation), it was possible to conveniently approximate and compare the phytochemical portfolios of those diverse plants. By factoring in the crop yields of different species, economically important conclusions can be reached—with pot marigold (C. officinalis) seemingly the most viable substitute for damask rose as a source of phenolics. Fatty acid and microelement analyses were also performed, to further enrich the chemical profiles of plant extracts. The paper also aims to collate and redesign multiple colorimetric assays frequently used while studying plant extracts in vitro, but criticized for their lack of correlation to in vivo activity. We show that they remain a viable tool for direct comparison of extraction methodologies, while highlighting their shortcomings.

scholarly journals THE STUDY OF PHYSICO-TECHNOLOGICAL FACTORS’ INFLUENCE ON THE OUTPUT OF LIPOPHILIC SUB-STANCES FROM MEDICINAL PLANT MATERIALS

2019 ◽  
pp. 299-305
Author(s):  
Ekaterina Aleksandrovna Degtyareva ◽  
Liliya Ivanovna Vyshnevska ◽  
Svetlana Vasil'yevna Garnaya ◽  
Ekaterina Aleksandrovna Kalko
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Experimental Studies ◽  
Optimal Conditions ◽  
Plant Materials ◽  
Lipophilic Compounds ◽  
Technological Factors ◽  
Soxhlet Apparatus ◽  
Β Carotene ◽  
Lipophilic Substances

Currently, the lipophilic fractions of well-known medicinal plants are still understudied despite of contains of unique groups of BAS (chlorophylls, carotenoids, tocopherols, sterols, unsaturated fatty acids, phospholipids, etc.), what is the actual task of modern pharmaceutical science. The aim of our work was to perform experimental studies for the choice of optimal conditions of the extraction of lipophilic compounds from the meal of pumpkin pulp. Extraction of lipophilic substances from the meal of pumpkin pulp was carried out in laboratory conditions in the Soxhlet apparatus by the method of circulation extraction. The weight loss on drying, the content of extractive lipophilic substances and the amount of carotenoids (in terms of β-carotene) were determined by compendial procedures. According to the results of the experimental work, the optimum humidity (not more than 7%) and drying temperature (+ 60 °C) of pumpkin pulp meal containing carotenoids were established. The obtained data are necessary for the development of technology of lipophilic pumpkin extract.

scholarly journals In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Puneet Singh ◽  
Yogesh Sharma ◽  
Ashutosh Sharma
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Local Market ◽  
Plant Materials ◽  
Whole Plant ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory ◽  
Convolvulus Pluricaulis

Aim: In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy. Material & Methods- The whole plant parts of Convolvulus pluricaulis Choisy were purchased from the local market. Whole plant materials were dried under shade and subjected to coarse powder for extraction process. Accurately weighed quantity of whole plant material was extracted using 95 % methanol by soxhlet apparatus for 72 h. Qualitative chemical tests of methanolic extracts were subjected to various chemical tests to detect various phytoconstituents. Solvent systems ethyl acetate: methanol: water (77:13:10) were found to be most satisfactory solvent system. After development of plates, they were air-dried and number of spots, color and Rf values were recorded. The % heamolysis was calculated by assuming the heamolysis produced by the control group as 100 %. Results: The preliminary phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds. At a concentration of 500 µg/ml, the extract produced 71.59% protection of RBC haemolysis as compared with 72.73% produced by prednisolone.  The methanolic extract of selected plant showed 39.70% inhibition. The Diclofenac sodium showed 55.88 % inhibition against denaturation of protein. Conclusion: In conclusion, it can be stated that the methanolic extract has beneficial effects in long lasting in membrane stabilizing method, inhibition of protein denaturation method and proteinase model. Keywords: In-Vitro, Anti-inflammatory Activity, Methanolic Extract, Convolvulus pluricaulis Choisy, Protein Denaturation Method

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