scholarly journals Effects of Rice Cultivar, Additional Fertilizer Application, and Harvest Time on 4-aminobutyric Acid (GABA) Content of Brown Rice : Potential Improvements to GABA-enriched Supplementary Foods

2015 ◽  
Vol 62 (9) ◽  
pp. 461-464
Author(s):  
Takayo Saikusa ◽  
Tetsuya Ishikawa ◽  
Kouji Higuchi ◽  
Motohiko Ishida
Keyword(s):  
Brown Rice ◽  
Rice Cultivar ◽  
Fertilizer Application ◽  
Aminobutyric Acid ◽  
Harvest Time ◽  
Gaba Content ◽  
Supplementary Foods

Twin-Screw Extrusion of Pre-Germinated Brown Rice: Physicochemical Properties and ?-Aminobutyric Acid Content (GABA) of Extruded Snacks

2011 ◽  
Vol 7 (4) ◽  
Author(s):  
Nirandorn Chanlat ◽  
Sirichai Songsermpong ◽  
Chulaluck Charunuch ◽  
Onanong Naivikul
Keyword(s):  
Brown Rice ◽  
Rice Flour ◽  
Expansion Ratio ◽  
Aminobutyric Acid ◽  
Screw Speed ◽  
Germinated Brown Rice ◽  
Twin Screw ◽  
Feed Moisture ◽  
Gaba Content ◽  
Brown Rice Flour

Pre-germinated brown rice (PGBR) from non-glutinous (KDML 105) and glutinous (RD 10) varieties was produced by a soaking method in cool water at 25°C for 48 h. After germination, increased crude fiber, reducing sugar (P ? 0.05) and the ?-aminobutyric acid (GABA) content (P ? 0.01) and decreased fat were observed. The extrusion was carried out in a twin-screw extruder by a central composite design (CCD) which studied the effect of feed moisture (15.6-22.3%), screw speed (264-434 rpm) and pre-germinated glutinous brown rice flour level (0-63.6%) on the physicochemical properties of extruded snacks (expansion ratio, density, hardness, brittleness, water absorption index (WAI), water solubility index (WSI)) and GABA content. Second-order polynomial models were computed and used to generate surface plots. Increasing the feed moisture increased the density (P ? 0.01) hardness and WAI, and decreased the expansion ratio, brittleness (P ? 0.01) and WSI (P ? 0.05). Increasing the screw speed increased the expansion ratio, brittleness, WAI and WSI, and decreased the density and hardness. Increasing the levels of pre-germinated glutinous brown rice flour increased the expansion ratio (P ? 0.01), brittleness, WSI (P ? 0.01) and the GABA content (P ? 0.01), and decreased the density, hardness (P ? 0.01) and WAI (P ? 0.01), while the feed moisture content and screw speed had no significant effect on the GABA content (P > 0.05). This knowledge can be used in the design of customer-oriented extruded PGBR products.

scholarly journals Study the appropriate conditions to obtain germinated brown rice with high biological activity

2019 ◽  
Vol 57 (3B) ◽  
pp. 116
Author(s):  
Van Anh Luu ◽  
Hang Thanh Nguyen ◽  
Giang Truong Nguyen
Keyword(s):  
Amylase Activity ◽  
Brown Rice ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Germinated Brown Rice ◽  
Bioactive Substances ◽  
High Biological Activity ◽  
Germination Process ◽  
Effects Of Ph ◽  
Gaba Content

ABSTRACT – QMFS 2019Germinated brown rice strains contain more bioactive substances than germinated regular rice ones, however germination conditions play an important role in the activity and the content of those substances. The proper germination process provides the optimized active ingredients from rice that can be used for the production of nutritious beverages. In this study, we investigated the effects of pH, temperature and incubation time in microaerobic culture condition on the change of bioactive substances in AnhDao brown rice. The optimal germination condition with pH at 3, temperature of 35 0C and time for 36h release 109.11U/g of the α-amylase activity, 17.22(U/g) of the enzyme glutamate decacboxylase (GAD), 1.38(U/g) of protease, 231.76mg/100g of GABA content and 21.9 (mgGAE/100g) of polyphenol from germinated AnhDao brown rice. In nutrient evaluation, germinated AnhDao brown rice contains 65.53% of starch, 2.49% of lipid, 9.13%of protein, 2.04% of reducing sugar, and 1.26% of ash.Key words: Germinated brown rice, bioactive substances, α-amylase, protease, glutamate decacboxylase (GAD), Gamma aminobutyric acid (GABA).

Preparation of γ-Aminobutyric Acid from Brown Rice in the Determination

2011 ◽  
Vol 396-398 ◽  
pp. 1628-1631
Author(s):  
Su Hong Li ◽  
Li Juan Wang ◽  
Tuo Ping Li ◽  
Zhong Li Jang ◽  
Jun Wei Wang
Keyword(s):  
Brown Rice ◽  
Extraction Process ◽  
Water Extraction ◽  
Aminobutyric Acid ◽  
Liquid Ratio ◽  
Extraction Condition ◽  
Gaba Content ◽  
Effects Of Temperature ◽  
Optimum Extraction ◽  
Orthogonal Tests

Water extraction and ethanol reflux extraction of γ-aminobutyric acid (GABA) in brown rice were compared with the yield and the recovery. In the water extraction, the effects of temperature and time on the extraction process of GABA were investigated, the results showed that the optimum extraction condition was incubated at 50°C for the 3h, and the GABA content was 30.73mg/100g, the recovery of the water extraction was lower, just 60.5% on average. The single factor and orthogonal tests of the extraction conditions were discussed for the ethanol reflux extraction. The result indicated that the best extraction condition is incubated in 70% ethanol, at 80°C, solid to liquid ratio 1:7 (g/mL), GABA content was 50.45 mg/100g, the recovery was 98.5%.

FURTHER STUDIES ON THE BINDING OF γ-AMINOBUTYRIC ACID BY BRAIN

1967 ◽  
Vol 45 (12) ◽  
pp. 1795-1807 ◽  
Author(s):  
Paula Strasberg ◽  
K. A. C. Elliott
Keyword(s):  
Rat Brain ◽  
Mitochondrial Fraction ◽  
Sodium Ion ◽  
The Other ◽  
Aminobutyric Acid ◽  
Sodium Ions ◽  
Ninhydrin Reaction ◽  
Gaba Content

Factors which can interfere with the paper chromatographic – ninhydrin method for determining γ-aminobutyric acid (GABA) are described. The GABA–ninhydrin reaction does not involve loss of CO2. GABA that is occluded in subcellular particles in plain sucrose homogenates of rat brain does not readily exchange with radioactive GABA in solution. The relevant particles are found mostly in the "mitochondrial fraction". These particles deteriorate with time and manipulations, and tend to lose much of their GABA content. The presence of sodium (but not of potassium, calcium, or magnesium) in the suspending medium allows considerably more GABA to be bound. The extra bound GABA is exchangeable with free labelled GABA. Sodium also promotes some exchange between free and occluded GABA. It is concluded from the present and previous results that in brain in vivo very little GABA exists in a freely diffusing situation. There are two forms of bound GABA. One of these is an occluded or storage form which does not readily exchange with free GABA though exchange is to some extent promoted by sodium ions. The other is a form which occurs only in the presence of sodium ion and is freely exchangeable with GABA in solution.

scholarly journals Changes in Functionality of Germinated and Non-Germinated Brown Rice Fermented by Bacillus natto

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2779
Author(s):  
Huei-Ju Wang ◽  
Lin Chang ◽  
Yu-Shiun Lin
Keyword(s):  
Crude Protein ◽  
Nutritional Value ◽  
Radical Scavenging ◽  
Brown Rice ◽  
Essential Amino Acids ◽  
Aminobutyric Acid ◽  
Crude Protein Content ◽  
Germinated Brown Rice ◽  
Bacillus Natto ◽  
Fermented Products

Germinated brown rice (GBR) is brown rice (BR) that has been germinated. GBR accumulates more nutrients and has a softer texture than BR. The aim of this study was to ferment GBR and BR using Bacillus natto and to investigate the functionality of the fermented products compared with white rice (WR) as a control. After fermentation with B. natto, the crude ash, total essential amino acids, and fat contents of each sample increased, while the crude protein content decreased. Moreover, the γ-aminobutyric acid and γ-oryzanol contents decreased, while the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging increased significantly in all fermented samples. The nattokinase activity (FU/g) of the fermented products was highest for GBR (43.11), followed by BR (19.62), and lowest for WR (12.24). Collectively, these results indicate that GBR fermented with B. natto yields better nutritional value and functional properties than fermented BR or WR.

The preventing method of browning and γ-aminobutyric acid (GABA) contained in Luffa cylindrica Roem. cultivated in Okinawa

2021 ◽  
Vol 4 (4) ◽  
pp. 251-258
Author(s):  
Hanagasaki Takashi ◽  
Keyword(s):  
Ascorbic Acid ◽  
Acid Solution ◽  
Room Temperature ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Luffa Cylindrica ◽  
Air Exposure ◽  
Ascorbic Acid Solution ◽  
Gaba Content ◽  
Prevention Method

Luffa (Luffa cylindrica Roem.) is a popular vegetable in Okinawa, and it has abundant nutrients, including γ-aminobutyric acid (GABA). We focused on GABA content in luffa, taking into consideration registering it as foods with functional claims in Japan. Besides, when selling cut luffa and frozen cut luffa at supermarkets, they are supposed to get browned due to air exposure and other causes. In the present study, we developed the prevention method of browning cut luffa and frozen cut luffa using 0.5 %, 1.0 %, 2.0 %, and 4.0 % ascorbic acid solution. It was found that 55 L of 4.0 % ascorbic acid solution could be used for soaking of 70 kg cut luffa to prevent browning, but GABA content decreased in food processing of luffa in the factory. Besides, GABA content in luffa fruits was found not to change during storage for 07 days at room temperature after harvest.

scholarly journals Accumulation γ-Aminobutyric Acid and Biogenic Amines in a Traditional Raw Milk Ewe’s Cheese

Foods ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 401 ◽  
Author(s):  
Rosanna Tofalo ◽  
Giorgia Perpetuini ◽  
Noemi Battistelli ◽  
Alessia Pepe ◽  
Andrea Ianni ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Raw Milk ◽  
Starter Cultures ◽  
Aminobutyric Acid ◽  
Microbial Composition ◽  
Qrt Pcr ◽  
A Value ◽  
Gaba Content ◽  
Gaba Accumulation ◽  
Polymerase Chain

The influence of calf (R1), kid (R2) and pig (R3) rennets on microbiota, biogenic amines (BAs) and γ-aminobutyric acid (GABA) accumulation in raw milk ewe’s cheeses was evaluated. Cheeses were investigated at different ripening times for their microbial composition, free amino acids (FAAs), BAs and GABA content. Moreover, the expression of tyrosine (tdc) and histidine (hdc) decarboxylases genes was evaluated by quantitative Real Time–Polymerase Chain Reaction (qRT-PCR). Microbial counts showed similar values in all samples. Pig rennet were cheeses were characterized by higher proteolysis and the highest values of BAs. The BAs detected were putrescine, cadaverine and tyramine, while histamine was absent. qRT-PCR confirmed this data, in fact hdc gene was not upregulated, while tdc gene expression increased over time in agreement with the increasing content of tyramine and the highest fold changes were detected in R3 cheeses. GABA showed the highest concentration in R2 cheeses reaching a value of 672 mg/kg. These results showed that the accumulation of BAs and GABA in Pecorino di Farindola is influenced by ripening time and type of coagulant. Further studies are required to develop starter cultures to reduce BAs content and improve health characteristics of raw milk ewe’s cheeses.

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