scholarly journals Antibacterial and Anticancer Activity of Ethnomedicinal Plants Used in the Jongilanga Community, Mpumalanga

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Lall N ◽  
De Canha MN ◽  
Reid A ◽  
Oosthuizen CB ◽  
Langhansova L ◽  
...  
Keyword(s):  
Mycobacterium Smegmatis ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Radical Scavenging ◽  
Docking Studies ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Value ◽  
Cox 2 ◽  
Antibacterial And Anticancer Activity

Seventy-four ethanolic extracts were prepared from traditionally used medicinal plants in the Jongilanga community in Mpumalanga South Africa. The aim was to determine the biological activity of the selected plants against cancer, mycobacteria species and acne. From the results, it was evident that Mundulea sericea was able to inhibit the proliferation of human melanoma cells (A375) with a fifty percent inhibitory concentration (IC50) ranging between 50 and 100 µg/ml as well as the ability to inhibit Mycobacterium tuberculosis, Mycobacterium smegmatis and Propionibacterium acnes with minimum inhibitory concentrations (MIC) of 125, 31.25 and 7.9 µg/ml respectively. This further led to the investigation of the antioxidant and anti-inflammatory activity as well as the influence of the extract on mycothiol disulphide reductase (Mtr) and glutathione reductase enzymes (Gtr) as potential targets against the above-mentioned diseases. M. sericea inhibited the COX-2 enzyme, responsible for inflammation, with an IC50 value of 10.70 ± 1.14 µg/ml, furthermore compounds previously isolated from M. sericea showed potential inhibition of COX-2 in molecular docking studies. Low radical scavenging capacity against the DPPH free radical with an IC50 value of 60.52 ± 2.40 µg/ml was obtained, however, M. sericea showed a higher affinity towards Mtr as compared to Gtr, which makes it an ideal plant for use as an antimycobacterial agent.

In-vitro Cytotoxic and Antioxidant Activity of Bauhinia racemosa Root

2021 ◽  
pp. 3333-3336
Author(s):  
Aravanan P. ◽  
Jayakumari S
Keyword(s):  
Antioxidant Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Inhibitory Concentration ◽  
Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Line Analysis ◽  
Mcf 7

Objective: To evaluate cytotoxic and antioxidant activity of the plant Bauhinia racemosa root by in-vitro cell line analysis. Methods: MCF-7 cell lines were used for the study to find the inhibitory concentration of the extract. DPPH and NO scavenging assays were performed to study the antioxidant nature of the extract. Results and Conclusion: In MCF-7 cell line cytotoxicity effect was observed in tested sample concentrations in 48 hours treatment, it also revealed that increased concentration of drug shown increased cytotoxicity over the MCF-7 cell lines. DPPH and NO scavenging Assay revealed a concentration dependent radical scavenging capacity of the tested sample concentrations of Bauhinia racemosa root spectrophotometrically.

scholarly journals Antioxidant, antifungal and anticancer activities of se-enriched Pleurotus spp. mycelium extracts

2014 ◽  
Vol 66 (4) ◽  
pp. 1379-1388 ◽  
Author(s):  
Ivan Milovanovic ◽  
Mirjana Stajic ◽  
Jasmina Cilerdzic ◽  
Tatjana Stanojkovic ◽  
Aleksandar Knezevic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Concentration ◽  
Radical Scavenging ◽  
Pleurotus Eryngii ◽  
Dpph Radical ◽  
Anticancer Activities ◽  
Radical Scavenging Capacity ◽  
Low Concentrations ◽  
Scavenging Capacity ◽  
Dpph Radical Scavenging

The goal of this study was the evaluation of antifungal, antioxidant and anticancer potentials of Pleurotus eryngii, P. ostreatus and P. pulmonarius mycelial extracts, and the influence of mycelium enrichment with selenium on these activities. Both Se-amended and non-amended extracts showed the same or similar minimal inhibitory concentration for 14 studied micromycetes, while a fungicidal effect was not noted, contrary to ketoconazole, which had inhibitory and fungicidal effects at very low concentrations. Se-non-amended extracts exhibited antioxidant activity, especially at higher concentrations. Selenium enrichment influenced activity, its effects decreasing in P. eryngii and P. pulmonarius, while in P. ostreatus no effect was noted. The DPPH? radical scavenging capacity of the extracts was in direct correlation with their phenol and flavonoid contents. Cytotoxic activity against both HeLa and LS174 cell lines was very low compared with cis-DDP. These features suggest that mycelium should be an object of intensive studies.

scholarly journals Quantification of Total Phenolics Content and Their Antioxidant Scavenging Capacity in Selected Herbs Extract

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Normah Haron ◽  
Noraslinda Muhammad Bunnori ◽  
Noor Hasniza Md Zin ◽  
Widya Abdul Wahab ◽  
Khairul Bariyyah Abdul Halim
Keyword(s):  
Total Phenolics ◽  
Radical Scavenging ◽  
Strong Correlations ◽  
Dpph Assay ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Phenolics Content ◽  
Gallic Acid Equivalent

Introduction: G. procumbens, H. sabdariffa and C. nutans contain potential compoundsthat lead to the development of drugs and supplements. The quantification of phenoliccompound was conducted by analyzing the compounds at 3 different fractions (free,insoluble bound and soluble bound). Thus, the objectives of this research were todetermine TPC and antioxidant of the plants in three different extracts. Methods: Threereplicates of the medicinal plants were extracted with 80% methanol and hydrolysis withHCl and NaOH. The TPC was determined by using Folin-Ciocalteau reagent whileantioxidant radical scavenging capacities were analyzed by using the DPPH assay. Results:Results showed that the TPC of the G. procumbens and C. nutans extracts of the insolublebound extract had the highest amount of phenolic compounds (15.19 ± 0.25 mg of GAE/gand 6.09+ 0.45 mg gallic acid equivalent GAE/g DW, respectively) as compared to theother fractions (p<0.05). In contrast, H. sabdariffa had the highest TPC value inboundsoluble phenolic compound (7.63 + 0.28 mg GAE/g DW) than the other fractions (p<0.05).The antioxidant scavenging capacity of the G. procumbens and C. nutans extracts showedthat highest IC50 values in the insoluble bound extract > free > soluble bound extract,whereas the bound soluble of H. sabdariffa showed highest IC50 value > free >boundinsoluble phenolic extracts. Conclusions: Strong correlations were found between TPCwith antioxidant radical scavenging capacity. This finding proved that these three plantscould be a good source of natural antioxidant for food, cosmetic and pharmaceuticalindustry.

scholarly journals Pengujian Kapasitas Antioksidan Wedang Tahu dalam Rangka Meningkatkan Mutu Fungsionalnya

2020 ◽  
Vol 7 (1) ◽  
pp. 46-51
Author(s):  
Nurheni Sri Palupi ◽  
◽  
Rachmat Widyanto ◽  
Keyword(s):  
Radical Scavenging ◽  
Health Benefit ◽  
Water Extract ◽  
Radical Scavenging Capacity ◽  
Improve Health Status ◽  
Scavenging Capacity ◽  
Ic50 Value ◽  
Dpph Method ◽  
Optimum Characteristic ◽  
Main Components

Efforts to prevent and overcome various degenerative diseases can be realized by developing functional foods that are known as natural or processed foods containing bioactive components that have the potential to improve health status. One of whole foods that has potency to be developed as functional food is soft tofu dessert. Soft tofu dessert has two main components which are soft tofu and ginger drink. However, there is lack of references providing the evidence of the characteristics and health benefit or soft tofu dessert. This study provide data to support the development of soft tofu dessert as a whole food that has optimum characteristic and contains benefit for health as well. The research objectives were to test radical scavenging capacity of soft tofu dessert and its component by implementing DPPH method. Antioxidant capacity of the samples was observed by using DPPH method (517 nm). Ranges of concentration were observed to determine IC50 of each sample. IC50 value presents the inhibition of 50% DPPH radical by samples. The values of IC50 for ginger water extract, ginger drink, soft tofu, and soft tofu dessert were 1592.40±22.30 ppm, 2764.26±90.29 ppm, 24657.50±2483.11 ppm, and 3356.04±415.54 ppm respectively. This result showed the best radical scavenging capacity was ginger water extract > ginger drink > soft tofu dessert, and > soft tofu. Soft tofu dessert performed better scavenging capacity than soft tofu itself, but still performed the lower capacity compared to ginger water extract and ginger drink.

EVALUATION BIOLOGICAL ACTIVITIES OF Piper Sp INTENDED FOR SKIN FORMULATION PRESERVATIVE

2015 ◽  
Vol 77 (2) ◽  
Author(s):  
Saidatul Husni Saidin ◽  
Norulaiman Yusoff ◽  
Fauziah Abdullah ◽  
Mastura Mohtar ◽  
Ong Boo Kean ◽  
...  
Keyword(s):  
Free Radical ◽  
Cell Lines ◽  
Inhibitory Concentration ◽  
Ph Value ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Normal Liver Cell ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Preservatives are substances added into product to prevent their premature spoilage. Preservative helps to prolong the product’s shelf life by protecting the product from microbial growth and oxidation process. In this study, the potential of a Piper sp. as preservative for cosmetic products is being investigated. The exploration begins with the screening of major phytochemical groups; flavonoid, steroid, alkaloid, saponin and triterpene. Evaluation of biological activities exhibited the plant possessed antimicrobial property towards two strains of Staphylococcus aureus and free radical scavenging capacity. The minimum inhibitory concentration values (MIC) for both S. aureus ATCC 25923 and S. aureus ATCC 6538 is 2500 mg/mL and 312.5 mg/mL respectively. Free radical scavenging capacity of the plant was determined via Superoxide scavenging assay and DPPH radical scavenging assay and the capacity values are 76.4+0.35% and 59.7+0.75% respectively. Cytotoxicity study was also carried out to determine the safety of Piper sp. where median inhibitory concentration (IC50) value for normal kidney cell lines (Vero) is 62.96 mg/mL and normal liver cell lines (WRL-68) is 69.91mg/mL. The extract of Piper sp. was formulated as cosmetic preservative ingredient especially for topical product. The specification of ingredient’s characteristic and performance were described based on colour, odour, pH value as well the solubility in product formulation and stability at 4°C, room temperature and 40°C.

scholarly journals STUDY ON ANTIOXIDANT ACTIVITY OF PHYTOESTROGEN EXTRACTS FROM SOY GERM

2021 ◽  
Vol 58 (6A) ◽  
pp. 270
Author(s):  
Chau Minh Le ◽  
Vien Thi Hoa Do ◽  
Ha Phu Ho
Keyword(s):  
Antioxidant Activity ◽  
Cardiovascular Health ◽  
Biological Effects ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Oxygen Species ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Value ◽  
Ranking Order

Soy germ is one of the richest phytoestrogen sources and thus has many benefits for health such as improving bone density, cardiovascular health, cancer prevention, and menopausal treatment. In addition, phytoestrogens are reported to act as antioxidants, removing reactive oxygen species and thereby preventing oxidative damage in living tissue. Phytoestrogens in soy germ include isoflavone compounds and their derivatives: daidzein, genistein, glycitein, daidzin, genistin, glycitin, acetyl daidzin, acetyl genistin, acetyl glycitin, malonyl daidzin, malonyl genistin, malonyl glycitin. Isoflavones aglycone forms comprise only about 2-5% of total isoflavones, however, they express more biological effects than the others. The objective of this study was to compare the antioxidant activity among three extracts: purified isoflavone aglycone extract, crude isoflavone aglycone extract and total phytoestrogen extract. The IC50 value of DPPH free radical scavenging capacity of purified isoflavone aglycone extract, crude isoflavone aglycone extract and total phytoestrogen extract were 0.763 ± 0.016; 3.345 ± 0.076; 6.142 ± 0.050 mg/ml, respectively. The IC50 value of reducing power activity of purified isoflavone aglycone extract, crude isoflavone aglycone extract and total phytoestrogen extract were 1.248 ± 0.024; 3.961 ± 0.172; 9.385 ± 0.272 (mg/ml). As our result, the ranking order of the antioxidant activity (from highest to lowest level) was purified isoflavone aglycone extract > crude isoflavone aglycone extract > total phytoestrogens extract.

scholarly journals Microwave heating effect on total phenolics and antioxidant activity of green and mature coconut water

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Abraham Jesús Arzeta-Ríos ◽  
Diana Guerra-Ramírez ◽  
Benito Reyes-Trejo ◽  
Ma. Carmen Ybarra-Moncada ◽  
Holber Zuleta-Prada
Keyword(s):  
Antioxidant Activity ◽  
Heating Temperature ◽  
Cocos Nucifera ◽  
Radical Scavenging ◽  
Coconut Water ◽  
Total Phenolic ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Maximum Temperatures ◽  
Increasing Temperature

AbstractCoconut water (Cocos nucifera L.) from fruits of two ripening stages (green and mature) was used to perform microwave heat treatments. Three different heating conditions with maximum temperatures of 70, 80 and 90 °C were tested in three holding times (0, 2 and 4 min). The Total Phenolic Content (TPC) using the Folin-Ciocalteu method and ABTS radical scavenging capacity were evaluated for each combination of coconut fruit age, heating temperature and holding time. It was observed that green coconut water exhibited significant differences (p < 0.05) compared to mature coconuts in terms of TPC (46.03 and 69.16 mg GAE/L, respectively) and ABTS radical scavenging capacity (422.31 and 549.1 μmol TE/L, respectively). An increase in TPC (up to 23%) and antioxidant activity (up to 19%) was also observed with increasing temperature in the range of 70–90 °C. Green coconut water showed the largest increase in both TPC and antioxidant activity.

scholarly journals A simple new method for aged seed utilization based on melatonin-mediated germination and antioxidant nutrient production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song Yu ◽  
Xuetian Zhu ◽  
Helin Yang ◽  
Lihe Yu ◽  
Yifei Zhang
Keyword(s):  
Total Phenolics ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Aged Seed ◽  
Phenolics Content ◽  
Dpph Radical Scavenging ◽  
Aged Seeds

AbstractSeed deterioration, coupled with a decrease in nutrients, is unavoidable following long-term storage, and these seeds are therefore used as livestock fodder. Here, we developed a simple, rapid and efficient method of producing high amounts of antioxidants from deteriorated seeds via melatonin-induced germination. Legume seeds were subjected to high humidity at 55 °C for 12–36 h to obtain aged seeds with a 40% germination rate and severely reduced antioxidant nutrition (total phenolics content, ferric reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging capacity). Aged seeds were then treated with 0.1 mM melatonin, resulting in the production of sprouts with a higher total phenolics content (fivefold), greater ferric reducing power (sevenfold) and greater DPPH radical scavenging capacity (twofold) compared to the aged seeds. These findings suggest that melatonin treatment efficiently converted aged seed reserve residues into antioxidant nutrients, providing an alternative use for deteriorated seeds in food production.

scholarly journals Quality and Antioxidant Properties of Cold-Pressed Oil from Blanched and Microwave-Pretreated Pomegranate Seed

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 712
Author(s):  
Tafadzwa Kaseke ◽  
Umezuruike Linus Opara ◽  
Olaniyi Amos Fawole
Keyword(s):  
Radical Scavenging ◽  
Total Phenolic ◽  
Yellowness Index ◽  
Cold Pressing ◽  
Microwave Pretreatment ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Pomegranate Seed ◽  
Cold Pressed

The present research studied the influence of blanching and microwave pretreatment of seeds on the quality of pomegranate seed oil (PSO) extracted by cold pressing. Pomegranate seeds (cv. Acco) were independently blanched (95 ± 2 °C/3 min) and microwave heated (261 W/102 s) before cold pressing. The quality of the extracted oil was evaluated with respect to oxidation indices, refractive index, yellowness index, total carotenoids content, total phenolic content, flavor compounds, fatty acid composition, and 2.2-diphenyl-1-picryl hydrazyl (DPPH) and 2.2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity. Blanching and microwave pretreatments of seeds before pressing enhanced oil yield, total phenolic content, flavor compounds, and DPPH and ABTS radical scavenging capacity. Although the levels of oxidation indices, including the peroxide value, free fatty acids, acid value, ρ-anisidine value, and total oxidation value, also increased, and the oil quality conformed to the requirements of the Codex Alimentarius Commission (CODEX STAN 19-1981) standard for cold-pressed vegetable oils. On the other hand, blanching and microwave heating of seeds decreased the pomegranate seed oil’s yellowness index, whilst the refractive index was not significantly (p > 0.05) affected. Even though both blanching and microwave pretreatment of seeds added value to the cold-pressed PSO, the oil extracted from blanched seeds exhibited lower oxidation indices. Regarding fatty acids, microwave pretreatment of seeds before cold pressing significantly increased palmitic acid, oleic acid, and linoleic acid, whilst it decreased the level of punicic acid. On the contrary, blanching of seeds did not significantly affect the fatty acid composition of PSO, indicating that the nutritional quality of the oil was not significantly affected. Therefore, blanching of seeds is an appropriate and valuable step that could be incorporated into the mechanical processing of PSO.

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