scholarly journals Electrospun Nanofibrous Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) With Antibacterial Activity

2019 ◽  
pp. 1-7
Author(s):  
Samir Abbad Andaloussi ◽  
Davy Louis Versace ◽  
Estelle Renard ◽  
Etienne Dessauw ◽  
Valerie Langlois
Keyword(s):  
Antiradical Activity ◽  
Layered Materials ◽  
Antibacterial Activities ◽  
E Coli ◽  
Cyclodextrin Inclusion Complexes ◽  
Cyclodextrin Inclusion ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Antibacterial Material ◽  
Scanning Electron

Elaboration of electrospun nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV)-based mats was implemented through two routes to obtain antibacterial material containing carvacrol, a phenolic compound extracted from essential oils. The first strategy relied on a direct blend of PHBHV and carvacrol/-cyclodextrin inclusion complexes, PHBHV (carvCD-IC), while the second approach implied the elaboration of multilayered scaffold via the introduction of poly(β-CD-epichlorohydrin), PolyCD. The PolyCD/carv-based mat was electrospun between two hydrophobic PHBHV mats. Scanning Electron Microscopy analysis of mats showed uniform and beadless fibers. The three layered materials [PHBHV-(PolyCD/carv)-PHBHV] is sufficient to ensure optimal antiradical activity (RSA of 88.6%) and exhibit interesting antibacterial activities against E. coli and S. aureus.

Material analysis techniques using the ion mill

1996 ◽  
Vol 54 ◽  
pp. 1034-1035
Author(s):  
J. P. Benedict ◽  
R. M. Anderson ◽  
S. J. Klepeis
Keyword(s):  
Polished Surface ◽  
Surface Material ◽  
Analysis Techniques ◽  
Material Analysis ◽  
Optical Inspection ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Argon Ions ◽  
The Impact ◽  
Scanning Electron

Ion mills equipped with flood guns can perform two important functions in material analysis; they can either remove material or deposit material. The ion mill holder shown in Fig. 1 is used to remove material from the polished surface of a sample for further optical inspection or SEM ( Scanning Electron Microscopy ) analysis. The sample is attached to a pohshing stud type SEM mount and placed in the ion mill holder with the polished surface of the sample pointing straight up, as shown in Fig 2. As the holder is rotating in the ion mill, Argon ions from the flood gun are directed down at the top of the sample. The impact of Argon ions against the surface of the sample causes some of the surface material to leave the sample at a material dependent, nonuniform rate. As a result, the polished surface will begin to develop topography during milling as fast sputtering materials leave behind depressions in the polished surface.

scholarly journals Pathological ATX3 Expression Induces Cell Perturbations in E. coli as Revealed by Biochemical and Biophysical Investigations

2021 ◽  
Vol 22 (2) ◽  
pp. 943
Author(s):  
Diletta Ami ◽  
Barbara Sciandrone ◽  
Paolo Mereghetti ◽  
Jacopo Falvo ◽  
Tiziano Catelani ◽  
...  
Keyword(s):  
Cell Envelope ◽  
Spinocerebellar Ataxia Type ◽  
Spinocerebellar Ataxia Type 3 ◽  
Intact Cells ◽  
E Coli ◽  
Fourier Transform Infrared Microspectroscopy ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
The Fourier Transform ◽  
Type 3

Amyloid aggregation of human ataxin-3 (ATX3) is responsible for spinocerebellar ataxia type 3, which belongs to the class of polyglutamine neurodegenerative disorders. It is widely accepted that the formation of toxic oligomeric species is primarily involved in the onset of the disease. For this reason, to understand the mechanisms underlying toxicity, we expressed both a physiological (ATX3-Q24) and a pathological ATX3 variant (ATX3-Q55) in a simplified cellular model, Escherichia coli. It has been observed that ATX3-Q55 expression induces a higher reduction of the cell growth compared to ATX3-Q24, due to the bacteriostatic effect of the toxic oligomeric species. Furthermore, the Fourier transform infrared microspectroscopy investigation, supported by multivariate analysis, made it possible to monitor protein aggregation and the induced cell perturbations in intact cells. In particular, it has been found that the toxic oligomeric species associated with the expression of ATX3-Q55 are responsible for the main spectral changes, ascribable mainly to the cell envelope modifications. A structural alteration of the membrane detected through electron microscopy analysis in the strain expressing the pathological form supports the spectroscopic results.

Apicoectomy and Scanning Electron Microscopy Analysis of an Implant Infected by Apical (Retrograde) Peri-implantitis: A Case Letter

2018 ◽  
Vol 44 (4) ◽  
pp. 287-291
Author(s):  
Rafael Manfro ◽  
Gislaine Felipe Garcia ◽  
Marcelo Carlos Bortoluzzi ◽  
Vinicius Fabris ◽  
Atais Bacchi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Scanning Electron Microscopy Analysis ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Scanning Electron

scholarly journals Influence of heat treatment on physicochemical and rheological characteristics of natural yogurts

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2489 ◽  
Author(s):  
Juliana Aparecida Célia ◽  
Marco Antônio Pereira da Silva ◽  
Kênia Borges de Oliveira ◽  
Diene Gonçalves Souza ◽  
Ligia Campos de Moura Silva ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Heat Treatment ◽  
Titratable Acidity ◽  
Physicochemical Characteristics ◽  
Rheological Characteristics ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Lactic Bacteria ◽  
Milk Treatment ◽  
Scanning Electron

The aim of this study was to assess the influence of heat treatment on physicochemical and rheological characteristics of natural yogurts, as well as the influence of lyophilization process on natural yogurts after reconstitution. In the first experiment, three yogurt treatments were processed, as follows: Treatment 1, yogurt produced with raw refrigerated milk; Treatment 2, yogurt produced with refrigerated pasteurized milk; and Treatment 3, yogurt produced with UHT (ultra-high temperature) milk, in addition to analyses of fat, protein, moisture, titratable acidity, and pH. The shelf life of yogurts at 1, 8, 15, 22, and 29 days of storage, as well as pH, acidity, syneresis, viscosity, viable lactic bacteria, and total coliforms were also assessed. In the second experiment, yogurts were submitted to lyophilization process, performed by scanning electron microscopy analysis and subsequently in those reconstituted, in addition to being assessed the physicochemical, rheological, and viable lactic bacteria characteristics. The results found in the first experiment showed that heat treatment was positive for viscosity, syneresis, and lactic bacteria, being viable until the 15th day of storage only for yogurts submitted to heat treatment. In the second experiment, lyophilization preserved the physicochemical characteristics of yogurts, but the number of initial lactic bacteria was different, also negatively affecting yogurt viscosity.

scholarly journals Residual smear layer after root canal instrumentation by using Niti, M-Wire and CM-Wire instruments: A scanning electron microscopy analysis

2018 ◽  
Vol 12 (03) ◽  
pp. 403-409 ◽  
Author(s):  
Ricardo Machado ◽  
Daniel Comparin ◽  
Eduardo Donato Eing Engelke Back ◽  
Lucas da Fonseca Roberti Garcia ◽  
Luiz Rômulo Alberton
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Root Canal ◽  
Smear Layer ◽  
Significant Difference ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Root Canal Instrumentation ◽  
Reciprocating Systems ◽  
Scanning Electron

ABSTRACT Objective: The purpose of this study was to compare the amount of residual smear layer after root canal instrumentation by using Ni-Ti, M-Wire, and CM-Wire instruments. Materials and Methods: Seventy-two mandibular incisors were randomly divided into six groups according to the system used: WaveOne (WO), Reciproc (RP), Unicone (UC), ProTaper Next (PN), Mtwo (MT), and HyFlex (HF). Afterward, the specimens were cleaved in the mesiodistal and buccolingual direction for analysis by scanning electron microscopy. Results: Considering both directions and root canal thirds, there was no difference between HF, MT, and PN. RP, UC, and WO presented a significant difference between the directions, and the cervical third showed a significantly smaller quantity of residual smear layer compared with the apical third. When the systems were compared among them, there was a significant difference only between RP and WO. Conclusions: Residual smear layer observed after instrumentation with the different systems was similar, except for quantities between the reciprocating systems.

Sign in / Sign up

Export Citation Format

Share Document