scholarly journals Sensitivity and specificity of infrared thermography in detection of subclinical mastitis in dairy cows

2010 ◽  
Vol 93 (8) ◽  
pp. 3525-3532 ◽  
Author(s):  
B. Polat ◽  
A. Colak ◽  
M. Cengiz ◽  
L.E. Yanmaz ◽  
H. Oral ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Infrared Thermography ◽  
Sensitivity And Specificity ◽  
Subclinical Mastitis

Udder skin surface temperature variation pre- and post- milking in dairy cows as determined by infrared thermography

2018 ◽  
Vol 85 (2) ◽  
pp. 201-203 ◽  
Author(s):  
Chunhe Yang ◽  
Gan Li ◽  
Xiaojun Zhang ◽  
Xianhong Gu
Keyword(s):  
Surface Temperature ◽  
Dairy Cows ◽  
Infrared Thermography ◽  
Sensitivity And Specificity ◽  
Milk Yield ◽  
Skin Surface ◽  
Optimal Time ◽  
Skin Surface Temperature ◽  
Infrared Images ◽  
Significant Difference

The objectives of the research reported in this Research Communication were to compare the variation of hind quarter skin surface temperature pre- and post- milking in dairy cows and to determine the optimal time to capture images by infrared thermography for improving the sensitivity and specificity of mastitis detection in dairy cows. Hind quarter infrared images of 102 Holstein dairy cows were captured from the caudal view by an infrared camera pre-milking and post-milking. The udder skin surface temperature was measured with the help of the image processing software. No significant difference was found between the left and right quarter skin surface temperature pre- and post- milking. The hind quarter skin surface temperature pre-milking was not significantly influenced by milk yield, but exhibited a rising trend along with the increase of milk yield. The hind quarter skin surface temperature post-milking was significantly influenced by milk yield. This leads us to conclude that the sensitivity and specificity of IRT in mastitis detection may be influenced by milk yield and it may be better to capture the infrared images of cow udders pre-milking.

scholarly journals Sensitivity of various methods (CMT, CE and Indicator Paper) of subclinical cattle’s mastitis diagnostic in some dairy cows breeding in east of Algeria

2018 ◽  
Vol 4 (2) ◽  
pp. 06
Author(s):  
Brahim Bouchoucha ◽  
Omar Bouaziz ◽  
Nourreddine Zeghilet ◽  
Rachida Aimer ◽  
Sana Hireche ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Dairy Cows ◽  
Sensitivity And Specificity ◽  
Infection Rate ◽  
Subclinical Mastitis ◽  
Bacteriological Examination ◽  
Ph Indicator ◽  
Specific Test ◽  
Specificity And Sensitivity ◽  
The One

The aim of present work is to look for the prevalence of subclinical mastitis in dairy  cows of different regions in eastern Algerian ; the prevalence of subclinical mastitis has called for three non-specific methods and by  bacteriological analysis. On all teats are 416 ; the first non-specific test is Californiamastitis test (CMT) indicating polymorphonuclear witness infection of the udder. The second test indicates presence of ions (Na Cl) in  milk and carries the appellation of the electrical conductivity of milk (EC) and the last test is the one that revealed the pH of milk while using papier pH containing pH indicator and changes color to green or blue if the udder is infected. It is the first component of the study; the second component is to get the bacteriological status of each udder  and passing to the study of the sensitivity and specificity of non specifics tests . the present  study  gave values of mastitis prevalence  between 6.7 to 64.7% of cows and 10 to 20% of udders  tested by (CMT, pH papers and EC), with an infection rate of 9% of districts and 17% of cows. Bacteriological examination of positive areas showed the prevalence of the following pathogens : 6 species  of  Staph –  Saprophyticus= 15%, 6 species  staph – epidermidis=15%, 4 species staph – cohnii=10%, 9 Staph +=22.5%, 2 Micrococcus. Spp=5%, 4 E.Coli=10%, 2 speciesof  klebsielle =5%,2Proteus vulgaris=5%, 2Citrobacter freundeii=5%, 1 Streptococcus spp=2.5%, 1Streptococcus uberis=2.5%. et 5% others specie of bacterias. for specificity and  sensitivity we have these values r : CMT = 71% and 77%;. For pH paper had values of 15% and 60% .in latter puts the EC with values of 13% and 67% respectively. So the CMT remains the most accessible and reliable tset

scholarly journals Comparison of cow-side diagnostic tests for subclinical mastitis of dairy cows in Musanze district, Rwanda

2017 ◽  
Vol 88 ◽  
Author(s):  
Blaise Iraguha ◽  
Humphrey Hamudikuwanda ◽  
Borden Mushonga ◽  
Erick Kandiwa ◽  
Jean P. Mpatswenumugabo
Keyword(s):  
Dairy Cows ◽  
Sensitivity And Specificity ◽  
Diagnostic Tests ◽  
Subclinical Mastitis ◽  
Kappa Statistics ◽  
Test Results ◽  
Substantial Agreement ◽  
Likelihood Ratios ◽  
California Mastitis Test ◽  
Strength Of Association

Four subclinical mastitis diagnostic tests (the UdderCheck® test [a lactate dehydrogenasebased test], the California Mastitis Test [CMT], the Draminski® test [a conductivity-based test] and the PortaSCC® test [a portable somatic cell count-based test]) were compared in a study comprising crossbreed dairy cows (n = 30) during September and October 2015. Sensitivity and specificity of the CMT, Draminski® and UdderCheck® tests were compared with the PortaSCC® as reference. The CMT, Draminski® and UdderCheck® test results were compared with the results of the PortaSCC® test using kappa statistics. Duplicate quarter milk samples (n = 120) were concurrently subjected to the four tests. Sensitivity and specificity were 88.46% and 86.17% (CMT), 78.5% and 81.4% (Draminski®) and 64.00% and 78.95% (UdderCheck®). The CMT showed substantial agreement (k = 0.66), the Draminski® test showed moderate agreement (k = 0.48) and the UdderCheck® test showed fair agreement (k = 0.37) with the PortaSCC® test and positive likelihood ratios were 6.40, 4.15 and 3.04, respectively. The cow-level subclinical mastitis prevalence was 70%, 60%, 60% and 56.7% for PortaSCC®, CMT, Draminski® and UdderCheck® tests, respectively. At udder quarter level, subclinical mastitis prevalence was 20%, 21.67% and 20.83% for PortaSCC®, CMT and UdderCheck®, respectively. A correlation (P < 0.05) and moderate strength of association were found between the four tests used. The study showed that compared to the PortaSCC® test, the CMT was the most preferable option, followed by the Draminski® test, while the UdderCheck® test was the least preferable option for subclinical mastitis screening.

Using infrared thermography to detect subclinical mastitis in dairy cows in compost barn systems

2021 ◽  
Vol 97 ◽  
pp. 102881
Author(s):  
Nítalo A.F. Machado ◽  
Lucas B.S. Da Costa ◽  
José A.D. Barbosa-Filho ◽  
Kenio P.L. De Oliveira ◽  
Lucas C. De Sampaio ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Infrared Thermography ◽  
Subclinical Mastitis

scholarly journals Infrared thermography: A potential noninvasive tool to monitor udder health status in dairy cows

2016 ◽  
Vol 9 (10) ◽  
pp. 1075-1081 ◽  
Author(s):  
M. Sathiyabarathi ◽  
S. Jeyakumar ◽  
A. Manimaran ◽  
G. Jayaprakash ◽  
Heartwin A. Pushpadass ◽  
...  
Keyword(s):  
Health Status ◽  
Dairy Cows ◽  
Infrared Thermography ◽  
Udder Health

Mass-spec-based urinary metabotyping around parturition identifies screening biomarkers for subclinical mastitis in dairy cows

2020 ◽  
Vol 129 ◽  
pp. 39-52
Author(s):  
Grzegorz Zwierzchowski ◽  
Guanshi Zhang ◽  
Rupasri Mandal ◽  
David S. Wishart ◽  
Burim N. Ametaj
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Mass Spec

Evaluation of the udder health status in subclinical mastitis affected dairy cows through bacteriological culture, somatic cell count and thermographic imaging

2015 ◽  
Vol 18 (4) ◽  
pp. 799-805 ◽  
Author(s):  
A. Bortolami ◽  
E. Fiore ◽  
M. Gianesella ◽  
M. Corrò ◽  
S. Catania ◽  
...  
Keyword(s):  
Health Status ◽  
Dairy Cows ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Bacteriological Culture ◽  
Udder Health ◽  
Streptococcus Uberis ◽  
Thermographic Imaging

Abstract Subclinical mastitis in dairy cows is a big economic loss for farmers. The monitoring of subclinical mastitis is usually performed through Somatic Cell Count (SCC) in farm but there is the need of new diagnostic systems able to quickly identify cows affected by subclinical infections of the udder. The aim of this study was to evaluate the potential application of thermographic imaging compared to SCC and bacteriological culture for infection detection in cow affected by subclinical mastitis and possibly to discriminate between different pathogens. In this study we evaluated the udder health status of 98 Holstein Friesian dairy cows with high SCC in 4 farms. From each cow a sample of milk was collected from all the functional quarters and submitted to bacteriological culture, SCC and Mycoplasma spp. culture. A thermographic image was taken from each functional udder quarter and nipple. Pearson’s correlations and Analysis of Variance were performed in order to evaluate the different diagnostic techniques. The most frequent pathogen isolated was Staphylococcus aureus followed by Coagulase Negative Staphylococci (CNS), Streptococcus uberis, Streptococcus agalactiae and others. The Somatic Cell Score (SCS) was able to discriminate (p<0.05) cows positive for a pathogen from cows negative at the bacteriological culture except for cows with infection caused by CNS. Infrared thermography was correlated to SCS (p<0.05) but was not able to discriminate between positive and negative cows. Thermographic imaging seems to be promising in evaluating the inflammation status of cows affected by subclinical mastitis but seems to have a poor diagnostic value.

scholarly journals Molecular characterization of virulence factors in Staphylococcus aureus isolated from bovine subclinical mastitis in central Ethiopia

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Desiye Tesfaye Tegegne ◽  
Gezahegne Mamo ◽  
Hika Waktole ◽  
Yohannes Equar Messele
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cows ◽  
Virulence Factors ◽  
Virulence Genes ◽  
Animal Health ◽  
Subclinical Mastitis ◽  
Biochemical Tests ◽  
Milk Samples ◽  
Lactating Dairy Cows ◽  
Genomic Study

Abstract Purpose Staphylococcus aureus (S. aureus) is the most important pathogen involved in bovine mastitis in dairy production. S. aureus produces a spectrum of extracellular protein toxins and virulence factors which are thought to contribute to the pathogenicity of the organism. The aim of this work was to isolate and molecular characterize S. aureus associated with bovine subclinical mastitis in the central part of Ethiopia. Methods A total of 265 lactating dairy cows from various dairy farms in four different geographical locations were screened by the California mastitis test (CMT) for bovine subclinical mastitis. One hundred thirty CMT-positive milk samples were collected and transported to the laboratory. Different biochemical tests and polymerase chain reaction (PCR) were used for the identification of S. aureus isolates. Finally, PCR was performed for molecular detection of virulence genes. Results From a total of 265 lactating dairy cows screened, 49% (n = 130) were positive for bovine subclinical mastitis. One hundred thirty mastitic milk samples were subjected to bacterial culturing, and one hundred (76%) S. aureus isolates were identified based on phenotypic characters. Sixty-eight confirmed S. aureus isolates were obtained using PCR. The confirmed S. aureus isolates were tested for six virulence genes (tsst-1, hlb, eta, sea, clfA, and icaD) using PCR. Of the six virulence genes screened from all the isolates, only two (clfA and eta) were detected in the isolates. Out of 68 isolates, 25% and 22% were possessed the eta and clfA genes, respectively. Conclusion The presence of Staphylococcus aureus having virulence genes (eta and clfA) revealed that mastitis is a major concern nowadays affecting animal health, milk quality, and yield. Further genomic study of these isolates will provide broad new insights on virulence.

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