scholarly journals Effect of abomasal glucose infusion on plasma concentrations of gut peptides in periparturient dairy cows

2010 ◽  
Vol 93 (12) ◽  
pp. 5729-5736 ◽  
Author(s):  
M. Larsen ◽  
A.E. Relling ◽  
C.K. Reynolds ◽  
N.B. Kristensen
Keyword(s):  
Dairy Cows ◽  
Plasma Concentrations ◽  
Glucose Infusion ◽  
Gut Peptides

Effects of metabolizable protein on intake and milk production of dairy cows independent of effects on ruminal digestion

2003 ◽  
Vol 76 (1) ◽  
pp. 137-146 ◽  
Author(s):  
P. Faverdin ◽  
D. M’hamed ◽  
R. Vérité
Keyword(s):  
Food Intake ◽  
Dairy Cows ◽  
Protein Content ◽  
Dry Matter ◽  
Plasma Concentrations ◽  
Glucose Infusion ◽  
Ruminal Fermentation ◽  
Direct Role ◽  
Metabolizable Protein ◽  
Ruminal Digestion

AbstractThe rôle of protein in food intake regulation is complex in ruminants. Previous research has shown that a deficiency in degradable nitrogen (N) could affect microbial activity and decrease intake. On the other hand, an increase in metabolizable protein content of the diet seems to stimulate food intake in lactating dairy cows. The aim of this experiment was to determine whether metabolizable protein supply plays a direct rôle in the stimulation of food intake. Treatments comprised two infusions of soya protein isolate (800 g/day) either into the rumen (RP) or into the duodenum (DP), which were compared with two iso-energy infusions of glucose (880 g/day) either into the rumen (RG) or into the duodenum (DG). Four ruminally and duodenally cannulated cows producing 36·5 kg/day of milk were assigned to a 4 ✕ 4 Latin-square design with periods of 4 weeks. Duodenal infusions of protein (DP) significantly increased (P < 0·05) dry-matter intake (DMI) ( +1·9 kg/day), rate of intake ( + 8·2 g DMI per min), milk yield ( + 4 kg/day), protein content ( + 2·3 g/kg) and protein yield ( +191 g/day) compared with the glucose infusion in the duodenum (DG). No significant effect was observed with ruminal infusion of protein (RP) compared with the glucose infusion in the rumen (RG). The protein infusions had no effect (P > 0·05) on the apparent digestibility of dry matter, organic matter, neutral-detergent fibre or acid-detergent fibre and also no or only small effects on ruminal fermentation variables. Plasma concentrations of most of the essential amino acids increased significantly with the duodenal infusion of protein, whereas ruminal infusion of protein had no significant effect. It is concluded that direct supply of metabolizable protein stimulates intake independently of ruminal digestion effects.

scholarly journals Effects of a Duodenal Glucose Infusion on the Relationship Between Plasma Concentrations of Glucose and Insulin in Dairy Cows

1997 ◽  
Vol 80 (11) ◽  
pp. 2854-2865 ◽  
Author(s):  
S. Lemosquet ◽  
N. Rideau ◽  
H. Rulquin ◽  
P. Faverdin ◽  
J. Simon ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Plasma Concentrations ◽  
Glucose Infusion ◽  
The Relationship

Effects of metoclopramide on duodenal motility and flow events, glucose absorption, and incretin hormone release in response to intraduodenal glucose infusion

2010 ◽  
Vol 299 (6) ◽  
pp. G1326-G1333 ◽  
Author(s):  
Paul Kuo ◽  
Max Bellon ◽  
Judith Wishart ◽  
André J. Smout ◽  
Richard H. Holloway ◽  
...  
Keyword(s):  
Blood Glucose Concentration ◽  
Hormone Secretion ◽  
Plasma Concentrations ◽  
Glucose Absorption ◽  
Glucose Infusion ◽  
Pressure Waves ◽  
Incretin Hormone ◽  
Duodenal Motility ◽  
Glucagon Like Peptide 1 ◽  
Gip Release

The contribution of small intestinal motor activity to nutrient absorption is poorly defined. A reduction in duodenal flow events after hyoscine butylbromide, despite no change in pressure waves, was associated with reduced secretion of the incretin hormones glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) and a delay in glucose absorption. The aim of this study was to investigate the effect of metoclopramide on duodenal motility and flow events, incretin hormone secretion, and glucose absorption. Eight healthy volunteers (7 males and 1 female; age 29.8 ± 4.6 yr; body mass index 24.5 ± 0.9 kg/m2) were studied two times in randomized order. A combined manometry and impedance catheter was used to measure pressure waves and flow events in the same region of the duodenum simultaneously. Metoclopramide (10 mg) or control was administered intravenously as a bolus, followed by an intraduodenal glucose infusion for 60 min (3 kcal/min) incorporating the14C-labeled glucose analog 3- O-methylglucose (3-OMG). We found that metoclopramide was associated with more duodenal pressure waves and propagated pressure sequences than control ( P < 0.05 for both) during intraduodenal glucose infusion. However, the number of duodenal flow events, blood glucose concentration, and plasma 3-[14C]OMG activity did not differ between the two study days. Metoclopramide was associated with increased plasma concentrations of GLP-1 ( P < 0.05) and GIP ( P = 0.07) but lower plasma insulin concentrations ( P < 0.05). We concluded that metoclopramide was associated with increased frequency of duodenal pressure waves but no change in duodenal flow events and glucose absorption. Furthermore, GLP-1 and GIP release increased with metoclopramide, but insulin release paradoxically decreased.

Intraruminal administration ofMegasphaera elsdeniimodulated rumen fermentation profile in mid-lactation dairy cows

2011 ◽  
Vol 79 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Qendrim Zebeli ◽  
Sarah J Terrill ◽  
Alberto Mazzolari ◽  
Suzanna M Dunn ◽  
Wen Z Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Plasma Concentrations ◽  
Rumen Fermentation ◽  
Holstein Cows ◽  
Plasma Metabolites ◽  
Fermentation Profile

This study evaluated the effects of intraruminal administration ofMegasphaera elsdeniion ruminal fermentation patterns, the profile of plasma metabolites, and milk yield and composition of mid-lactation dairy cows. Eight primiparous, ruminally cannulated Holstein cows were arranged in a paired 2×2 crossover design. Cows were randomly assigned to one of two treatments: 1) intraruminal inoculation of 35 ml suspension per day ofM. elsdeniiATCC 25940 (MEGA), containing 108cfu/ml of bacteria, dissolved in 35 ml of saline (0·15m), or 2) carrier alone (35 ml saline; CTR). Both postprandial and preprandial rumen volatile fatty acids (VFA) and plasma metabolite measurements were analysed. Postprandial VFA patterns were affected the most, with butyrate (P<0·01) and valerate (P<0·01) proportions increasing, and acetate (P<0·01), isobutyrate (P=0·05) and isovalerate (P<0·01) decreasing in MEGA cows. Preprandial data measured at various days showed that MEGA dosage tended to increase the molar proportion of propionate (P=0·09) and lower the acetate to propionate ratio (P=0·07) in the rumen fluid. There was no effect of treatment on rumen pH and on the concentration of lactate in the rumen as well as on selected preprandial plasma metabolites. Postprandial plasma concentrations of cholesterol tended to increase (P=0·07) in MEGA cows compared with CTR. Concentrations of non-esterified fatty acids (NEFA) in the plasma were lower in MEGA cows after the morning feeding (P<0·01). Sampling hour also affected plasma NEFA in this study. Plasma β-hydroxybutyrate (BHBA) were not affected by the treatment (P>0·05); however, after the morning feeding BHBA concentration was increased in both groups of cows. Dry matter intake and milk yield and composition were not affected by treatment. In conclusion, results indicate thatM. elsdeniihas the potential to modulate the rumen fermentation profile in mid-lactation Holstein cows, but these effects were only slightly reflected in changes in plasma metabolites and milk composition.

Changes in plasma concentrations of S100A7 and S100A8 in dairy cows during pregnancy

2018 ◽  
Vol 53 (4) ◽  
pp. 1013-1015 ◽  
Author(s):  
RA Elgawish ◽  
Y Ogata ◽  
T Hidaka ◽  
T Nii ◽  
Y Yoshimura ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Plasma Concentrations

Relationships between nutrition and fertility in dairy cattle

2001 ◽  
Vol 26 (1) ◽  
pp. 147-159 ◽  
Author(s):  
D. O'Callaghan ◽  
J.M. Lozano ◽  
J. Fahey ◽  
V. Gath ◽  
S. Snijders ◽  
...  
Keyword(s):  
Food Intake ◽  
Dietary Intake ◽  
Dairy Cows ◽  
Embryo Development ◽  
Milk Production ◽  
Body Condition ◽  
Developmental Process ◽  
Plasma Concentrations ◽  
Metabolic State ◽  
Developmental Capacity

AbstractThe reduced fertility that is becoming more evident in high yielding dairy cows may be related to many factors including changes in milk production, food intake and fluctuations in body condition. Metabolic and production markers have been studied as a way of predicting success to a particular artificial insemination. Successful conception to a particular service was not associated with milk production, body condition or plasma concentrations of several indicators of metabolic state around the time if insemination. This highlights the importance of time of information collection in fertility management programmes. Increased food intake may reduce systemic progesterone concentrations. This is more evident in sheep than cattle, but a positive relationship between systemic progesterone early post mating and establishment of pregnancy in cattle has been reported. However, progesterone concentrations in the ovarian vein and endometrium are not strongly correlated with systemic progesterone. Thus, the significance of modest changes in systemic progesterone in affecting oocyte and embryo development must be questioned. Blood urea concentrations can be altered by diet, and reduced pregnancy rates have been reported in cows with high urea concentrations. However, in other recent studies, no difference was reported in serum urea in cows that conceived and those that failed to conceive. Pregnancy rate was equally high in heifers when in-vitro produced embryos were transferred to heifers on high and low urea diets. When embryos were produced in sheep on high and low dietary urea, the effects on embryo development appear to occur early in the developmental process, suggesting a substantial effect on the development of the oocyte. The developmental capacity of oocytes and quality of embryos is reduced in cattle maintained on extremely high dietary intakes. Oocyte developmental capacity is reduced in cows of higher genetic merit and embryo quality can be substantially reduced in the early postpartum period. Collectively, these results suggest that high dietary intake or high metabolic load is deleterious to normal oocyte development and establishment of pregnancy. This highlights the importance of further studies on the effect of dietary intake on metabolic state and follicle, oocyte and embryo development. In a practical context, these results highlight the importance of nutritional management and avoiding changes in the amount or type of diet around the time of mating in high-production dairy cows.

Glucose regulates lipid metabolism in fasting king penguins

2003 ◽  
Vol 285 (2) ◽  
pp. R313-R320 ◽  
Author(s):  
Servane F. Bernard ◽  
Jord Orvoine ◽  
René Groscolas
Keyword(s):  
Lipid Metabolism ◽  
Plasma Concentration ◽  
Energy Production ◽  
Important Determinant ◽  
Plasma Concentrations ◽  
Glucose Infusion ◽  
Nonesterified Fatty Acids ◽  
Fat Stores

This study aims to determine whether glucose intervenes in the regulation of lipid metabolism in long-term fasting birds, using the king penguin as an animal model. Changes in the plasma concentration of various metabolites and hormones, and in lipolytic fluxes as determined by continuous infusion of [2-3H]glycerol and [1-14C]palmitate, were examined in vivo before, during, and after a 2-h glucose infusion under field conditions. All the birds were in the phase II fasting status (large fat stores, protein sparing) but differed by their metabolic and hormonal statuses, being either nonstressed (NSB; n = 5) or stressed (SB; n = 5). In both groups, glucose infusion at 5 mg·kg-1·min-1 induced a twofold increase in glycemia. In NSB, glucose had no effect on lipolysis (maintenance of plasma concentrations and rates of appearance of glycerol and nonesterified fatty acids) and no effect on the plasma concentrations of triacylglycerols (TAG), glucagon, insulin, or corticosterone. However, it limited fatty acid (FA) oxidation, as indicated by a 25% decrease in the plasma level of β-hydroxybutyrate (β-OHB). In SB, glucose infusion induced an ∼2.5-fold decrease in lipolytic fluxes and a large decrease in FA oxidation, as reflected by a 64% decrease in the plasma concentration of β-OHB. There were also a 35% decrease in plasma TAG, a 6.5- and 2.8-fold decrease in plasma glucagon and corticosterone, respectively, and a threefold increase in insulinemia. These data show that in fasting king penguins, glucose regulates lipid metabolism (inhibition of lipolysis and/or of FA oxidation) and affects hormonal status differently in stressed vs. nonstressed individuals. The results also suggest that in birds, as in humans, the availability of glucose, not of FA, is an important determinant of the substrate mix (glucose vs. FA) that is oxidized for energy production.

scholarly journals Conception Rate and Reproductive Hormone Secretion in Holstein Cows Immunized against Inhibin and Subjected to the Ovsynch Protocol

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 313 ◽  
Author(s):  
Rihong Guo ◽  
Fang Chen ◽  
Cheng Mei ◽  
Zicun Dai ◽  
Leyan Yan ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Reproductive Performance ◽  
Luteal Phase ◽  
Low Dose ◽  
Follicle Stimulating Hormone ◽  
Hormone Secretion ◽  
Plasma Concentrations ◽  
High Dose ◽  
Reproductive Hormone ◽  
Conception Rates

This study was conducted to investigate the feasibility of improving fertility in dairy cows via immunization against inhibin. Thirty-two cows were divided into Control (n = 11), Low-dose (n = 10) and High-dose (n = 11) groups. The High-dose and Low-dose cows were treated with 1 and 0.5 mg of the inhibin immunogen, respectively. All the cows were subjected to the Ovsynch protocol from the day of antigen administration and were artificially inseminated. Blood samples were serially collected over a 24-day period from the start of the Ovsynch protocol to 14 days after insemination. The results showed that immunization against inhibin dose-dependently increased the plasma concentrations of follicle-stimulating hormone (FSH), estradiol (E2), and activin A, but decreased progesterone (P4) concentrations in the luteal phase. Immunization also increased the plasma interferon (IFN)-τ concentrations in pregnant cows on day 14 after initial insemination. The conception rates in High-dose (45.5%) and Low-dose (40%) cows marginally increased compared to that in Control cows (27.3%), but the increases were not significant (p > 0.05). In conclusion, a single immunization against inhibin has the potential to improve conception rates, despite impaired luteal development. To further improve the reproductive performance of dairy cows, additional luteal-stimulating treatments are suggested in combination with immunization against inhibin and Ovsynch techniques.

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