scholarly journals Resting-state functional MRI in an intraoperative MRI setting: proof of feasibility and correlation to clinical outcome of patients

2016 ◽  
Vol 125 (2) ◽  
pp. 401-409 ◽  
Author(s):  
Constantin Roder ◽  
Edyta Charyasz-Leks ◽  
Martin Breitkopf ◽  
Karlheinz Decker ◽  
Ulrike Ernemann ◽  
...  
Keyword(s):  
Functional Mri ◽  
Negative Correlation ◽  
Resting State ◽  
Clinical Condition ◽  
Characteristic Curve ◽  
Significant Negative Correlation ◽  
Intraoperative Mri ◽  
Neurological Deficits ◽  
Z Score ◽  
Z Scores

OBJECTIVE The authors' aim in this paper is to prove the feasibility of resting-state (RS) functional MRI (fMRI) in an intraoperative setting (iRS-fMRI) and to correlate findings with the clinical condition of patients pre- and postoperatively. METHODS Twelve patients underwent intraoperative MRI-guided resection of lesions in or directly adjacent to the central region and/or pyramidal tract. Intraoperative RS (iRS)–fMRI was performed pre- and intraoperatively and was correlated with patients' postoperative clinical condition, as well as with intraoperative monitoring results. Independent component analysis (ICA) was used to postprocess the RS-fMRI data concerning the sensorimotor networks, and the mean z-scores were statistically analyzed. RESULTS iRS-fMRI in anesthetized patients proved to be feasible and analysis revealed no significant differences in preoperative z-scores between the sensorimotor areas ipsi- and contralateral to the tumor. A significant decrease in z-score (p < 0.01) was seen in patients with new neurological deficits postoperatively. The intraoperative z-score in the hemisphere ipsilateral to the tumor had a significant negative correlation with the degree of paresis immediately after the operation (r = −0.67, p < 0.001) and on the day of discharge from the hospital (r = −0.65, p < 0.001). Receiver operating characteristic curve analysis demonstrated moderate prognostic value of the intraoperative z-score (area under the curve 0.84) for the paresis score at patient discharge. CONCLUSIONS The use of iRS-fMRI with ICA-based postprocessing and functional activity mapping is feasible and the results may correlate with clinical parameters, demonstrating a significant negative correlation between the intensity of the iRS-fMRI signal and the postoperative neurological changes.

scholarly journals Characterization of Resting State Default Mode Network in Individuals with HbSS and HbSC, and Healthy Controls Using 7-Tesla Human MRI

Blood ◽  
2021 ◽  
Vol 138 (Supplement 1) ◽  
pp. 126-126
Author(s):  
Nadim Farhat ◽  
Helmet Karim ◽  
Tales Santini ◽  
Caterina Rosano ◽  
Leticia Candra ◽  
...  
Keyword(s):  
Functional Mri ◽  
Negative Correlation ◽  
Default Mode Network ◽  
Resting State ◽  
Ad Hoc ◽  
Brain Aging ◽  
Significant Negative Correlation ◽  
7 Tesla ◽  
Default Mode ◽  
Significant Difference

Abstract Sickle cell disease (SCD) is complicated by accelerated brain aging and cerebrovascular complications leading to structural brain damage and altered resting state functional connectivity (RS-FC). Investigation of the Default Mode Network (DMN), the most studied of the RS-FC networks, by functional MRI may provide insight into the neurological and neuropsychiatric complications of SCD. Prior studies have largely focused on the HbSS genotype of SCD as opposed to the HbSC genotype, which accounts for 30% of the total SCD cases in the US. Both genotypes are expected to impact brain disease, but in different manner. The aim of this study was to characterize the DMN in patients with HbSC, HbSS and healthy race and age-matched controls using state of the art 7-Tesla (7T) magnetic resonance imaging (MRI) with high contrast- and signal- to-noise ratios. On the day of the MRI scanning, participants were administered the brief pain inventory questionnaire (BPI), the digit symbol substitution test of cognitive function, and underwent basic blood work. Controls (n= 43, 53% female, mean age = 36+/-12 years), patients with HbSS (n=19, 63% female, mean age= 36+/-12) and HbSC (n=16 (50% female, mean age=36+/-13) were scanned using a 7T Siemens Magnetom scanner with the Tic Tac Toe radiofrequency coil system (Ibrahim et al., 2013). We obtained whole brain structural T1-weighted images and blood oxygenations level dependent resting state functional MRI (RS-fMRI). During the RS-fMRI, participants were instructed to keep their eyes open, look at a fixation cross, and lie without motion in the scanner. To identify significant differences in RS-FC between the groups, we conducted voxel-wise nonparametric analysis of variance. In the cortical areas with significant clusters, we ran ad-hoc t-tests (controls vs. HbSS, HbSC vs. HbSS, 1000 permutations, uncorrected cluster forming threshold = p &lt; 0.001, family-wise error (FWE) rate=0.05) and we added age and sex as confounders. Then, we correlated RS-FC and the following variables: age, hemoglobin, BPI total, and DSST score. We found a significant difference between the three groups in the DMN (Fig 1) (p=0.0002). The ad-hoc t-tests showed that in the DMN, the RS-FC for the medial prefrontal cortex (mPFC) was significantly higher for the controls when compared to patients with HbSS (p=0.002). RS-FC for the mPFC was also significantly higher in patients with HbSC as compared to HbSS (p=0.001). There was a non-significant trend towards lower RS-FC in patients with HbSC as compared to controls. We also found weak non-significant negative correlation, (r= -0.24, p=0.1315), a strong significant negative correlation (r=-0.5, p=0.04) and very strong significant negative correlation (r=-0.8, p=0.001) between age and RS-FC in the mPFC for controls, HbSC, and HbSS patients, respectively. (Fig 2). We did not find any significant correlation between the RS-FC in the three groups and Hb levels, BPI total, and DSST score. In this work and for the first time, using 7T human MRI we identified altered RS-FC between SCD genotypes and between controls and patients with HbSS. It is notable that the RS-FC for HbSC patients was intermediate between that of the other two groups. Our results imply the RS-FC levels might be susceptible to disease genotype and not necessarily Hb levels, cognition and pain. The finding of a stronger correlation between age and FC in patients with SCD as compared to controls corroborates the notion that accelerated brain aging is operant in SCD. Figure 1 Figure 1. Disclosures Novelli: Novartis Pharmaceuticals: Consultancy.

scholarly journals Nutritional role of amniotic fluid: clues from infants with congenital obstruction of the digestive tract

2018 ◽  
Vol 104 (2) ◽  
pp. F199-F201 ◽  
Author(s):  
Nigel J Hall ◽  
Melanie Drewett ◽  
David Burge
Keyword(s):  
Amniotic Fluid ◽  
Digestive Tract ◽  
Gestational Age ◽  
Significant Negative Correlation ◽  
Control Group ◽  
Z Score ◽  
Term Infants ◽  
Z Scores ◽  
And Gender

AimsTo investigate the role played by amniotic fluid in late fetal nutrition by analysis of infants born with digestive tract atresia.MethodsBirth weight (BW), gestational age and gender of infants born with oesophageal (OA), duodenal (DA), jejunal (JA) and ileal atresia (IA) were recorded and BW Z-scores compared. Infants with incomplete obstruction (stenosis), chromosomal or syndromic conditions and multiple congenital malformations were excluded. Term infants admitted with suspected postnatal intestinal obstruction in whom no congenital malformation was found were used as a control group.ResultsA total of 584 infants were identified comprising 148 OA, 60 DA, 26 JA and 57 IA with 293 in the control group. Infants with OA and DA had statistically significantly lower BW Z-score than controls. However, BW Z-score for infants with more distal atresia (JA and IA) was similar to controls. When compared with infants with OA, BW Z-score for infants with more distal atresia was higher than that for OA. BW Z-score in infants with OA was significantly lower in those born at term compared with those born preterm (mean±SD −0.92±1.0 vs −0.48±0.87; p=0.01) with a significant negative correlation between BW Z-score and increasing gestational age (R2=0.12; p<0.0001). This effect of gestational age was not seen in other atresias.ConclusionThese observations support the concept that reduced enteral absorption of amniotic fluid due to high digestive tract obstruction in utero reduces fetal growth. The effect is greater when the obstruction is more proximal and with advancing gestation.

scholarly journals Serum Biomarkers of Environmental Enteric Dysfunction and Growth Perspective in Egyptian Children

2021 ◽  
Vol 9 (B) ◽  
pp. 1625-1632
Author(s):  
Maged A. El Wakeel ◽  
Ghada M. El-Kassas ◽  
Shaimaa A. Hashem ◽  
Hasanin M. Hasanin ◽  
Walaa H. Ali ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin A ◽  
Negative Correlation ◽  
Serum Biomarkers ◽  
Control Group ◽  
Z Score ◽  
Positive Correlation ◽  
Egyptian Children ◽  
Environmental Enteric Dysfunction ◽  
Z Scores

BACKGROUND: Environmental enteric dysfunction (EED) is a chronic subclinical condition, contributed to limited sources and poor countries. EED pathology is concerned with small intestine structure and function, which affect the macronutrients and micronutrients absorption with consequent growth faltering. AIM: This study aimed to evaluate some serum biomarkers involved in EED and determine their association with stunting and faltering growth in children; zonulin, endotoxin core antibody (EndoCAb), high-sensitive C-reactive protein (hsCRP), alpha-1-acid glycoprotein (AGP), and tumor necrosis factor (TNF), serum iron, and Vitamins A and D. PATIENTS AND METHODS: This case–control study enrolled 105 children aged from 1 to 10 years old, having weight-for-age z-scores and height-for-age z-scores (WAZ or HAZ) ranging from −1.5 to −2. They were compared with control group consisted of 100 children having WAZ or HAZ > −1 of matched age and sex. Assessment of serum markers levels of enteric dysfunction (zonulin and EndoCAb), markers of systemic inflammation (Hs CRP and AGP), along with serum micronutrients (vitamin A, vitamin D and iron) in children with malnutrition in comparison to controls. RESULTS: There was a highly significant decrease as regarding the anthropometric measurements; weight, height, BMI, and arm circumference. Moreover, significant increase in serum zonulin, EndoCAb, HsCRP, and AGP and highly significant decrease of serum Vitamin D and iron in cases group as compared to control group. Height Z score showed negative correlation with zonulin, HsCRP, and AGP and positive correlation with Vitamin D. Weight Z score showed negative correlation with zonulin, HsCRP, and AGP and positive correlation with Vitamin D and Vitamin A. Regression analysis noted increase of zonulin and α1AGP as high associative markers with height Z score affection, however, increase of zonulin was high associative markers with weight Z score affection. CONCLUSION: Faltering growth is associated with elevated serum systemic markers of intestinal inflammation (HsCRP and α1AGP). EED may be a cause of faltering growth.

scholarly journals Serum Biomarkers of Environmental Enteric Dysfunction and Growth Perspective in Egyptian Children

2020 ◽  
Vol 8 (T2) ◽  
pp. 246-253
Author(s):  
Maged El Wakeel ◽  
Ghada El-Kassas ◽  
Shimaa Hashem ◽  
Hasanin Mohamed ◽  
Walaa Ali ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin A ◽  
Negative Correlation ◽  
Serum Biomarkers ◽  
Control Group ◽  
Z Score ◽  
Positive Correlation ◽  
Egyptian Children ◽  
Environmental Enteric Dysfunction ◽  
Z Scores

BACKGROUND: Environmental enteric dysfunction (EED) is a chronic subclinical condition, contributed to limited sources and poor countries. EED pathology is concerned with small intestine structure and function, which affect the macronutrients and micronutrients absorption with consequent growth faltering. AIM: This study aimed to evaluate some serum biomarkers involved in EED and determine their association with stunting and faltering growth in children; zonulin, endotoxin core antibody (EndoCAb), high-sensitive C-reactive protein (hsCRP), alpha-1-acid glycoprotein (AGP), and tumor necrosis factor (TNF), serum iron, and Vitamins A and D. PATIENTS AND METHODS: This case–control study enrolled 105 children aged from 1 to 10 years old, having weight-for-age z-scores and height-for-age z-scores (WAZ or HAZ) ranging from −1.5 to −2. They were compared with control group consisted of 100 children having WAZ or HAZ > −1 of matched age and sex. Assessment of serum markers levels of enteric dysfunction (zonulin and EndoCAb), markers of systemic inflammation (Hs CRP and AGP), along with serum micronutrients (vitamin A, vitamin D and iron) in children with malnutrition in comparison to controls. RESULTS: There was a highly significant decrease as regarding the anthropometric measurements; weight, height, BMI, and arm circumference. Moreover, significant increase in serum zonulin, EndoCAb, HsCRP, and AGP and highly significant decrease of serum Vitamin D and iron in cases group as compared to control group. Height Z score showed negative correlation with zonulin, HsCRP, and AGP and positive correlation with Vitamin D. Weight Z score showed negative correlation with zonulin, HsCRP, and AGP and positive correlation with Vitamin D and Vitamin A. Regression analysis noted increase of zonulin and α1AGP as high associative markers with height Z score affection, however, increase of zonulin was high associative markers with weight Z score affection. CONCLUSION: Faltering growth is associated with elevated serum systemic markers of intestinal inflammation (HsCRP and α1AGP). EED may be a cause of faltering growth.

scholarly journals P1675BONE DENSITOMETRY IN RENAL TRANSPLANTED PATIENTS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Ana Carina Ferreira ◽  
Marco Mendes ◽  
Cecília Silva ◽  
Patrícia Cotovio ◽  
Inês Aires ◽  
...  
Keyword(s):  
Femoral Neck ◽  
Negative Correlation ◽  
Bone Biopsy ◽  
Agatston Score ◽  
Z Score ◽  
Vascular Calcifications ◽  
X Ray ◽  
T Score ◽  
Neck Fracture ◽  
Z Scores

Abstract Background and Aims Renal transplant and associated immunosuppression can influence bone volume. Presently, the data is conflicting with older studies showing bone loss after transplant, while recent ones didn’t conclude the same. The aim of this study was to analyse the relations between bone-related molecules [phosphorus (Pi), Calcium (Ca), Magnesium (Mg), parathyroid hormone (PTH), bone alkaline phosphatase (bAP), calcitonin, vitamin D (vitD), alpha-klotho, fibroblast grow factor (FGF) 23, sclerostin] and bone densitometry findings in renal transplanted patients. Method We performed a prospective cohort study of a consecutive sample of de novo single renal transplanted patients in our unit. At inclusion, demographic, clinical and transplant-related data were collected, X-ray of the pelvis and hands (Adragão score) and echocardiographic findings were recorded. All patients were submitted to a laboratorial evaluation and a bone biopsy at baseline (time 0). Patients were followed for 12 months, after which performed laboratorial evaluation, 2nd bone biopsy, echocardiogram, X-ray of pelvis and hands, bone densitometry (DXA) and non-contrast cardiac CT for Agatston score (time 1). Continuous variables are presented as medians and categorical variables as frequencies. Associations between variables were performed using Wilcoxon matched-pairs test and Spearman correlation test. STATA software was used and p &lt; 0.05 was considered statistically significant. Results We recruited 85 patients from 1st October 2015 to 1st March 2018. At the end of 12 months, 6 patients refuse to perform the 2nd evaluation, 5 had primary non-function of the kidney graft, 1 had no 1st bone biopsy sample in time 0 and 4 patients died. We performed a 2nd evaluation in 69 patients and included those in this study. Mean age 50.2±12.4 years, 48 men, 53 caucasian (78.8%), median BMI 24.5 (22.7 – 27.8), median dialysis vintage 55 months (42 – 84). Patients had a median cumulative steroid dose of 5692.5 (5260 – 7250) mg. At 12 months, the median FRAX value for osteoporotic fracture was 3.5 (2.2 – 6.2) and for femoral neck fracture was 0.8 (0.2 – 2.7). The DXA findings are shown in Table 1. We found a negative correlation between vascular calcifications (Agatston Score and respective calcium percentile) and T and Z score of femoral neck (p=0.04), but not with the other DXA variables. Total femur variables (DMO, T-score, Z-score) were correlated with sclerostin values in time 1 (p&lt;0.01), and there was a trend for correlation between the spine DXA variables (DMO, T-score, Z-score) and sclerostin in time 1 (p=0.08, p=0.07, p=0.04). Spine DXA variables were negatively associated with alpha-klotho in time 0 (p=0.04, p=0.05, p=0.06). We didn’t found correlations with Pi, Ca or bAP. There was a negative correlation between FRAX osteoporotic and femoral neck fracture values and alpha-klotho in time 1 (p=0.002; p=0.003). Conclusion We found an inverse correlation between T and Z scores of femoral neck and coronary vascular calcifications. Regarding bone-derived hormones, Spine T and Z scores and FRAX values negatively correlated with alfa-klotho. Sclerostin seems to be associated with high mineral density.

scholarly journals Quantitative Electroencephalogram Standardization: A Sex- and Age-Differentiated Normative Database

2021 ◽  
Vol 15 ◽  
Author(s):  
Juhee Ko ◽  
Ukeob Park ◽  
Daekeun Kim ◽  
Seung Wan Kang
Keyword(s):  
Resting State ◽  
Normative Data ◽  
Quantitative Eeg ◽  
Z Score ◽  
Data Set ◽  
Normative Database ◽  
Confounding Variables ◽  
Z Scores ◽  
Quantitative Electroencephalogram ◽  
Standardized Index

We describe the utility of a standardized index (Z-score) in quantitative EEG (QEEG) capable of when referenced to a resting-state, sex- and age-differentiated QEEG normative database (ISB-NormDB). Our ISB-NormDB comprises data for 1,289 subjects (553 males, 736 females) ages 4.5 to 81 years that met strict normative data criteria. A de-noising process allowed stratification based on QEEG variability between normal healthy men and women at various age ranges. The ISB-NormDB data set that is stratified by sex provides a unique, highly accurate ISB-NormDB model (ISB-NormDB: ISB-NormDB-Male, ISB-NormDB-Female). To evaluate the trends and accuracy of the ISB-NormDB, we used actual data to compare Z-scores obtained through the ISB-NormDB with those obtained through a traditional QEEG normative database to confirm that basic trends are maintained in most bands and are sensitive to abnormal test data. Finally, we demonstrate the value of our standardized index of QEEG, and highlight it’s capacity to minimize the confounding variables of sex and age in any analysis.

Thrombin generation in paediatric patients with congenital heart disease

2008 ◽  
Vol 28 (S 01) ◽  
pp. S61-S66 ◽  
Author(s):  
G. Cvirn ◽  
A. Rosenkranz ◽  
B. Leschnik ◽  
W. Raith ◽  
W. Muntean ◽  
...  
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Negative Correlation ◽  
Congenital Heart ◽  
Thrombin Generation ◽  
Significant Negative Correlation ◽  
Peak Height ◽  
Global Test ◽  
Paediatric Patients ◽  
Age Range

SummaryThrombin generation was studied in paediatric patients with congenital heart disease (CHD) undergoing cardiac surgery using the calibrated automated thrombography (CAT) in terms of the lag time until the onset of thrombin formation, time to thrombin peak maximum (TTP), endogenous thrombin potential (ETP), and thrombin peak height. The suitability to determine the coagulation status of these patients was investigated. Patients, material, methods: CAT data of 40 patients with CHD (age range from newborn to 18 years) were compared to data using standard coagulation parameters such as prothrombin (FII), antithrombin (AT), tissue factor pathway inhibitor (TFPI), prothrombin fragment 1.2 (F 1.2), thrombin-antithrombin (TAT), activated partial thromboplastin time (aPTT), and prothrombin time (PT). Results: A significant positive correlation was seen between ETP and FII (p < 0.01; r = 0.369), as well as between peak height and F II (p < 0.01; r = 0.483). A significant negative correlation was seen between ETP and TFPI values (p < 0.05; r = –0.225) while no significant correlation was seen between peak height and TFPI. A significant negative correlation was seen between F 1.2 generation and ETP (p < 0.05; r = –0.254) and between F 1.2 generation and peak height (p < 0.05; r = –0.236). No correlation was seen between AT and ETP or peak. Conclusions: CAT is a good global test reflecting procoagulatory and inhibitory factors of the haemostatic system in paediatric patients with CHD.

Negative Correlation Between Serum Levels of Homocysteine and Apolipoprotein M

2019 ◽  
Vol 19 (2) ◽  
pp. 120-126
Author(s):  
J. Wei ◽  
Y. Yu ◽  
Y. Feng ◽  
J. Zhang ◽  
Q. Jiang ◽  
...  
Keyword(s):  
Negative Correlation ◽  
Hepg2 Cells ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
Mrna Levels ◽  
Rt Pcr ◽  
Apolipoprotein M ◽  
Protein Mass ◽  
Protein Levels ◽  
Phosphoinositide 3 Kinase

Background: Homocysteine (Hcy) has been suggested as an independent risk factor for atherosclerosis. Apolipoprotein M (apoM) is a constituent of the HDL particles. The goal of this study was to examine the serum levels of homocysteine and apoM and to determine whether homocysteine influences apoM synthesis. Methods: Serum levels of apoM and Hcy in 17 hyperhomocysteinemia (HHcy) patients and 19 controls were measured and their correlations were analyzed. Different concentrations of homocysteine (Hcy) and LY294002, a specific phosphoinositide 3- kinase (PI3K) inhibitor, were used to treat HepG2 cells. The mRNA levels were determined by RT-PCR and the apoM protein mass was measured by western blot. Results: We found that decreased serum apoM levels corresponded with serum HDL levels in HHcy patients, while the serum apoM levels showed a statistically significant negative correlation with the serum Hcy levels. Moreover, apoM mRNA and protein levels were significantly decreased after the administration of Hcy in HepG2 cells, and this effect could be abolished by addition of LY294002. Conclusions: resent study demonstrates that Hcy downregulates the expression of apoM by mechanisms involving the PI3K signal pathway.

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