CHANGES IN THE CELLULAR IMMUNE PROTECTION OF ORGANISM IN THE PROCESS OF CHRONIZATION OF EXPERIMENTAL BACTERIAL-IMMUNE PERIODONTITIS

One of the important factors that leads to damage of structures of the periodontal complex and leads to the formation of inflammatory process of varying degrees is the disruption of immunological processes. The aim of the study was to clarify the pathogenetic role of cellular adaptive immunity in the process of formation of chronic inflammatory reaction in the late period of the experimental bacterial-immune periodontitis. The study was conducted on white, non-breeding, clinically healthy male rats. Experimental bacterial-immune periodontitis in experimental animals was caused by insertion into the tissues of the periodontal complex a mixture of microorganisms diluted with egg protein. The obtained digital data was statistically processed using parametric and nonparametric statistical methods. The article represents the results of research on the parameters of cellular immune defense, determined by the relative number of CD3+ (common T-lymphocytes), CD4+ (T-helpers), CD8+ (cytotoxic cells, T-killers), CD19+ (B-lymphocytes), CD16+ (natural killers, NK-cell) and immunoregulatory index (CD4+ / CD8+) in intact animals and on the 30th day of experimental bacterial-immune periodontitis development. It was established that the nature of the course of experimental inflammation in the tissues of the periodontal complex depended on changes in the cellular immune status, accompanied by a decrease of the content of common mature T-lymphocytes (CD3+) in the blood of animals with experimental bacterial-immune periodontitis on the 30th day of the study. In the process of the development of the experimental bacterial-immune periodontitis there was a decrease of the content of T-lymphocytes-helper (CD4+) in the blood of animals and on the 30th day of the study an increase in T-suppressors (CD8+), an increase in the content of natural killers (CD16+) and a decrease in the relative content of B-lymphocytes (CD19+). The immunoregulatory index (CD4+ / СD8+) decreased in comparison with this indicator of a group of intact animals. In rats with bacterial-immune periodontitis, an immunosuppressive state developed in the late period of the inflammatory reaction due to both T-helper cells and cytotoxic T-suppressors / killers. These changes can be considered as signs of formation of the chronic course of the inflammatory process in the tissues of periodontal complex.

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CLINICAL EFFECTS OF HUMAN IMMUNOGLOBULIN ADMINISTRATION IN WOMEN WITH RHESUS SENSITIZATION AT THE PRE-GRAVID STAGE

Актуальні питання педіатрії акушерства та гінекології ◽

10.11603/24116-4944.2019.2.10913 ◽

2020 ◽

pp. 126-132

Author(s):

S. G. Chernievskaya ◽

N. M. Rozhkovska ◽

V. G. Marichereda ◽

T. O. Yermolenko

Keyword(s):

T Lymphocytes ◽

B Lymphocytes ◽

Cellular Immunity ◽

Roc Analysis ◽

Human Immunoglobulin ◽

Control Group ◽

Clinical Group ◽

Clinical Effects ◽

T Helper ◽

T Suppressors

The aim of the study – to evaluate changes in cellular immunity in rhesus-sensitized women in response to IVIG administration and the prognostic effectiveness of a method for the prevention of isoimmunization in the next pregnancy. Materials and Methods. The study was performed on the basis of City Maternity Hospital No. 7" (Odesa) in 2014–2019. 37 rhesus-sensitized women were randomly splited in two clinical groups: main clinical group (n=19) where patients received human immunoglobulin for intravenous administration, and control group (n=18) where patients did not receive IVIG. Results and Discussion. The state of cellular immunity in rhesus-sensitized women is characterized by a moderate decrease in the absolute and relative indices of T-lymphocytes while increasing the number of B-lymphocytes. The NK cell population did not differ from the control group. When analyzing subpopulations of T-lymphocytes, it can be concluded that the number of T-helper cells is increased and the number of T-suppressors is proportionally reduced. These changes explain the increase in the number of B-lymphocytes as a result of increasing antigenic load on cell receptors. In the group of women who received IVIG therapy, the ratio of chances of normalization of cellular immunity was 18.41 (95 % CI 2.62–166.74), T-helper – 14.93 (95 % CI) 2.45–107.8), T-suppressors – 14.57 (95 % CI 2.13 –127.57) and B-lymphocytes – 31.87 (95 % CI 4.1–333.41). According to the ROC analysis, the quality of the statistical model of IVIG application corresponds to AUC = 0.843 (95 % CI 0.689–0.941) According to the ROC analysis, the level of β-lymphocytes in the compared AUC groups = 0.58 (95 % CI 0.405–0.742).

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Immunophenotype of the inflammatory response in the central and enteric nervous systems of cockatiels (Nymphicus hollandicus) experimentally infected with parrot bornavirus 2

Veterinary Pathology ◽

10.1177/03009858211069166 ◽

2022 ◽

pp. 030098582110691

Author(s):

Jeann Leal de Araújo ◽

Raquel R. Rech ◽

Aline Rodrigues-Hoffmann ◽

Paula R. Giaretta ◽

Cinthya Cirqueira ◽

...

Keyword(s):

Small Intestine ◽

T Lymphocytes ◽

B Lymphocytes ◽

Inflammatory Infiltrate ◽

Homogeneous Distribution ◽

Nymphicus Hollandicus ◽

Inflammatory Infiltrates ◽

Cellular Immune ◽

Proventricular Dilatation Disease ◽

The Brain

Proventricular dilatation disease is a lethal disease of psittacine birds. In this study, we characterized the local cellular immune response in the brain, proventriculus, and small intestine of 27 cockatiels ( Nymphicus hollandicus) experimentally infected with parrot bornavirus 2 (PaBV-2). Perivascular cuffs in the brain were composed of CD3+ T-lymphocytes and Iba1+ macrophages/microglia in most cockatiels (n = 26). In the ganglia of the proventriculus, CD3+ T-lymphocytes (n = 17) and Iba1+ macrophages (n = 13) prevailed. The ganglia of the small intestine had a more homogeneous distribution of these leukocytes, including PAX5+ B-lymphocytes (n = 9), CD3+ T-lymphocytes (n = 8), and Iba1+ macrophages (n = 8). Our results indicate that perivascular cuffs in the brain and the inflammatory infiltrate in the proventriculus of PaBV-2-infected cockatiels is predominately composed of T-lymphocytes, while the inflammatory infiltrates in the ganglia of the small intestine are characterized by a mixed infiltrate composed of T-lymphocytes, B-lymphocytes, and macrophages.

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Research onTrypanosoma cruziand Analysis of Inflammatory Infiltrate in Esophagus and Colon from Chronic Chagasic Patients with and without Mega

Journal of Tropical Medicine ◽

10.1155/2012/232646 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 12

Author(s):

Eliângela de Castro Côbo ◽

Thales Parenti Silveira ◽

Adilha Misson Micheletti ◽

Eduardo Crema ◽

Sheila Jorge Adad

Keyword(s):

T Lymphocytes ◽

B Lymphocytes ◽

Myenteric Plexus ◽

Inflammatory Process ◽

Inflammatory Infiltrate ◽

Inflammatory Infiltration

To compare parasitism and inflammatory process in esophagus and colon from chronic chagasic patients, immunohistochemistry was carried out to research forT. cruziand to evaluate the inflammatory infiltrate in the muscular and myenteric plexus in 39 esophagi (20 with and 19 without megaesophagus) and 50 colons (25 with and 25 without megacolon). The frequency ofT. cruziin megaesophagus was 20%, and in megacolon it was 4%. No amastigotes were found in organs without mega; considering the total of esophagi (with and without mega), the frequency ofT. cruziwould be 10% and 2% in the colon. Myositis and ganglionitis were more frequent and intense in organs with mega compared to those without mega, and in esophagus compared to colon. Qualitatively, inflammatory infiltration in esophagus and colon, with or without mega, was similar, consisting predominantly of T lymphocytes (CD3+), scarce macrophages (CD68+), and rare B lymphocytes (CD20+).

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Influence of cadmium on the cellular part of the immune system of young cattle

Ukrainian Journal of Veterinary and Agricultural Sciences ◽

10.32718/ujvas3-2.08 ◽

2020 ◽

Vol 3 (2) ◽

pp. 47-52

Author(s):

Y. Y. Lavryshyn ◽

B. V. Gutyj ◽

K. Y. Leskiv ◽

I. I. Hariv ◽

L. H. Yevtukh ◽

...

Keyword(s):

Immune System ◽

T Lymphocytes ◽

B Lymphocytes ◽

Cadmium Chloride ◽

Control Group ◽

T Suppressors ◽

Wide Range ◽

Young Cattle ◽

The Impact ◽

Experimental Group

The article presents the results of research on the effect of cadmium load on the body of young cattle. The negative effect of Cadmium on the liver causes a wide range of pathological changes at different levels of its organization. The impact of heavy metals on the immune system is particularly significant, because it performs a leading role in maintaining health and is recognized as one of the most sensitive to adverse factors, even in relatively low concentrations. The aim of the study was to investigate the influence of cadmium on the cellular part of the immune system of young cattle. The research was carried out on 10 bulls of six months of age, Ukrainian black-and-white dairy breed, which were formed into 2 groups of 5 animals each: control and experimental. The bulls of the control group were on a normal diet. Animals of the experimental group were administered cadmium chloride at a dose of 0.04 mg/kg body weight of the animal. It was found that feeding bulls with a diet of cadmium chloride, the number of B-lymphocytes on the 5th day of the experiment was 17.54 ± 0.95 %. The lowest number of B-lymphocytes was on the 20th day of testing – 15.12 ± 0.37 %. The study of the number of T-lymphocytes shows that at the beginning of analysis the number of T-lymphocytes in the blood of animals of the control and experimental groups ranged from 40.70 ± 3.62 and 40.85 ± 2.54 %. Subsequently, the number of T-lymphocytes in the blood of the experimental group began to decline. The lowest number of T-lymphocytes was in the experimental group of animals on the 20th day of the research, compared with the control group, this figure decreased by 3.63 %. The state of immunity of animals under cadmium load significantly depends on the ratio of T-helpers to T-suppressors. It was found that the immunoregulatory index of blood of bulls of the experimental group probably decreased from 10 days of the research. The number of T-helper lymphocytes in the blood of the experimental group of bulls under cadmium loading is probably reduced by 15, 20 and 30 days of testing. A probable increase in the number of T-suppressors was noted in the bull's blood of the experimental group on the 20th day of the research. Immunoglobulins of different classes are crucial among bull’s serum proteins under cadmium loading. The concentration of immunoglobulins in the blood under cadmium load decreased by 15.9 % relative to the control group on the 20th day of analysis.

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Cellular Immune Suppressor Activity Resides in Lymphocyte Cell Clusters Adjacent to Granulomata in Human Coccidioidomycosis

Infection and Immunity ◽

10.1128/iai.73.7.3923-3928.2005 ◽

2005 ◽

Vol 73 (7) ◽

pp. 3923-3928 ◽

Cited By ~ 26

Author(s):

Lijin Li ◽

Sharon M. Dial ◽

Monika Schmelz ◽

Margaret A. Rennels ◽

Neil M. Ampel

Keyword(s):

T Lymphocytes ◽

B Lymphocytes ◽

Lymphocyte Subsets ◽

Interleukin 10 ◽

Suppressor Activity ◽

Ifn Γ ◽

Cellular Immune ◽

Lymphocyte Cell ◽

Immune Suppressor

ABSTRACT The in situ immunologic response in human coccidioidomycosis remains undefined. To explore this further, pulmonary necrotizing coccidioidal granulomata were examined using immunohistochemical staining for lymphocyte subsets and for the cytokines interleukin-10 (IL-10) and gamma interferon (IFN-γ). Discrete perigranulomatous lymphocytic clusters were seen in eight of nine tissues examined. In these tissues, T lymphocytes (CD3+) significantly outnumbered B lymphocytes (CD20+) in the mantle area of the granulomata (P = 0.028), whereas the clusters were composed of roughly equal numbers of T and B lymphocytes. While the number of cells in the mantle expressing IL-10 was similar to those in the perigranulomatous clusters, there were significantly more cells expressing IFN-γ in the mantle than in the clusters (P = 0.037). Confocal microscopy revealed that CD4+ T lymphocytes and B lymphocytes are associated with IL-10 production. CD4+CD25+ T lymphocytes were identified in the perigranulomatous clusters but were not associated with IL-10 production. This is the first report noting perigranulomatous lymphocyte clusters and IL-10 in association with human coccidioidal granulomata and suggests that down-regulation of the cellular immune response is occurring within coccidioidal granulomata.

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Determination of clinical efficiency of deproteinized dialysate from blood of milk-fed calves after endoprosthetics in patients with ventral hernias

I P Pavlov Russian Medical Biological Herald ◽

10.23888/pavlovj2020283323-333 ◽

2020 ◽

Vol 28 (3) ◽

pp. 323-333

Author(s):

Sergej V. Ivanov ◽

Ilya S. Ivanov ◽

Evgenij G. Obyedkov ◽

Liliya P. Popova

Keyword(s):

Abdominal Wall ◽

Inflammatory Reaction ◽

Inflammatory Process ◽

Anterior Abdominal Wall ◽

Dynamic Change ◽

Main Group ◽

Control Group ◽

Surgical Department ◽

Regeneration Stage

Aim. To study the influence of deproteinized dialysate from blood of milk-fed calves on the type of exudative discharge and dynamics of inflammatory reaction after hernia repair with plastics of the anterior abdominal wall with hernioendoprosthesis of polypropylene. Materials and Methods. The study involved 59 patients being on stationary treatment in the surgical department of Kursk Regional Clinical Hospital. Patients were hospitalized for the herniation of small or medium dimension. The patients were divided to two groups: the main (n=30) and control (n=29) groups. After endoprosthetics, the patients of the control group were given complex conservative treatment. The patients of the main group, besides standard treatment, were administered deproteinized dialysate from blood of milk-fed calves intravenously in drips 10 ml + 200 ml of 0.9% sodium chloride solution within 7 days. For cytological examination and determination of the type of cytograms, the traumatic discharge was collected and analyzed using the method of M.F. Kamaev and M.A. Palthsev. Results. Cytomorphometric examination was conducted on the third, fifth and seventh day after endoprosthetics with the aim of studying dynamic changes. Determination of cell composition and also of its changes characteristic of each studied period, was necessary for obtaining further information characterizing inflammatory process in the region of placement of the endoprosthesis. After endoprosthetics in patients who were administered hemodialysate, the inflammatory reaction was less pronounced than in patients who did not receive the preparation. This was associated with a more dynamic change of stages of the inflammatory process. In patients of the main group who received deproteinized dialysate, regenerative type of inflammation first appeared on the fifth day and made 6.9%; by the seventh day the share of patients with the regenerative type rose to 17.5%, while in the patients of the control group no regeneration stage was observed in both periods. Conclusion. Analysis of the efficiency of influence of hemodialysate on the inflammatory reaction in plastics of the anterior abdominal wall with polypropylene endoprosthesis evidences faster course of all stages of inflammation and reduction of its intensity by 10%.

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High levels ofbcl-2 protein in circulating t lymphocytes, but not b lymphocytes, of patients with systemic lupus erythematosus

Arthritis & Rheumatism ◽

10.1002/art.1780371004 ◽

1994 ◽

Vol 37 (10) ◽

pp. 1423-1430 ◽

Cited By ~ 70

Author(s):

Martin Aringer ◽

Winfried Wintersberger ◽

Carl W. Steiner ◽

Hans Kiener ◽

Elisabeth Presterl ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

T Lymphocytes ◽

B Lymphocytes ◽

Lupus Erythematosus ◽

Systemic Lupus

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T lymphocyte-dependent B lymphocyte proliferative response to antigen. I Genetic restriction of the stimulation of B lymphocyte proliferation.

Journal of Experimental Medicine ◽

10.1084/jem.153.4.871 ◽

1981 ◽

Vol 153 (4) ◽

pp. 871-882 ◽

Cited By ~ 20

Author(s):

H Y Tse ◽

J J Mond ◽

W E Paul

Keyword(s):

T Lymphocytes ◽

B Lymphocytes ◽

T Lymphocyte ◽

Lymphocyte Proliferation ◽

B Lymphocyte ◽

Antigen Specificity ◽

Apparent Lack ◽

The Face ◽

Stimulation Of

For the purpose of examining more closely the interaction between T and B lymphocytes, we have developed an in vitro T lymphocyte-dependent B lymphocyte proliferation assay. Proliferation of B lymphocytes in response to antigen was found to depend on the presence of primed T lymphocytes; the B lymphocytes could be derived from nonprimed animals. It appears that these B cells were nonspecifically recruited to proliferate. This nonspecific recruitment, however, was found to be Ir-gene restricted in that B lymphocytes from B10.S mice, which are genetic nonresponders to the polymer Glu60-Ala30-Tyr10 (GAT), could not be stimulated by GAT-primed (responder X nonresponder) F1 T cells. The apparent lack of antigen specificity in the face of Ir gene-restricted T-B interaction may have important implications in our understanding of the recognition unit(s) on T lymphocytes.

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