scholarly journals Study on Stem Cutting Propagation of Gynostemma Pentaphyllum (Thunb.) Makino in Hoa Binh Province

2021 ◽  
Vol 4 (2) ◽  
pp. 1034-1042
Author(s):  
Ninh Thi Phip ◽  
Nguyen Phuong Mai ◽  
Nguyen Thi Thanh Hai ◽  
Nguyen Mai Thom
Keyword(s):  
Growth And Development ◽  
Stem Cutting ◽  
Stem Cuttings ◽  
Mountainous Areas ◽  
Gynostemma Pentaphyllum ◽  
Black Sand ◽  
Cutting Length ◽  
Cropping Seasons ◽  
Over Exploitation ◽  
Hoa Binh

Gynostemma pentaphyllum (Thunb.) Makino is a medicinal plant distributed mostly in the mountainous areas of Vietnam. G. pentaphyllum contains more than 189 types of saponin which can be used for the treatment of various diseases. Currently, due to over-exploitation, this plant species is under threaten. Our research aimed to study a suitable method for plant propagation by stem cutting techniques to promote the growth and development of G. pentaphyllum. Six experiments were conducted to (i) evaluate the established seedling rates of different G. pentaphyllum varieties, and choose the best variety to (ii) evaluate the established seedling rate influenced by different factors, viz. stem cutting position, cutting season, growing in the selected substrates, stem cutting length, number of buds per stem, and application of α-NAA. The results showed that among five varieties, G2 which was collected in Mai Chau district disclosed the highest ability to shooting, the highest number of roots per stem cutting, the shortest nursery days, the highest shooting rate, and the highestestablished seedling rate. We also determined that using middle-aged plants for stem cuttings, at 15-20cm in length, with 2 nodes and grown in Spring and Winter cropping seasons, using growing substrates with a mixture of 50% of black sand and 50% of soil, and plus α-NAA 50ppm in 3-5min produced the highest explant rate. The established seedlingsat the time of explant were 15-20cm in height, containing 4-5 leaves and at least 9-10 roots per cutting, and were 30-32 days.

scholarly journals Pengaruh Panjang dan Diameter Stek Batang Terhadap Pertumbuhan Bibit Kelor (Moringa oleifera Lam.)

2018 ◽  
Vol 4 (2) ◽  
pp. 120
Author(s):  
Wahyu Astiko ◽  
Ahsani Taqwim ◽  
Bambang Budi Santoso
Keyword(s):  
Moringa Oleifera ◽  
Stem Diameter ◽  
Stem Cutting ◽  
Stem Length ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Cutting Length ◽  
Nursery Field ◽  
Moringa Oleifera Lam

The purpose of the study is to discover the best length and the diameter of Moringa oleifera Lam. stem cuttings to produce high quality of seedlings. The study was conducted during March-May 2017 located in the nursery field at Dasan Agung, Mataram, 16 m asl. This study was experimental research with Complete Randomized Design with three three replications, and each contains 5 serial experiment units. The treatment for stem cutting length (25 cm, 50 cm, and 75 cm) was then combined with different stem cutting diameters (3-4 cm, 4.1-5 cm, and 5.1-6 cm). The result shows that combination of 75 cm stem length with stem  diameter of 5.1-6 cm resulted to have the best seedling growth.The longer the cutting (up to 75 cm), the better the growth of the seedling. The thickest stem diameter (5.1-6 cm) resulted better seedling

scholarly journals Shooting of giant reed (Arundo donax L.) stem cuttings in cold greenhouse

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Piergiorgio Gherbin ◽  
Simone Milan ◽  
Giuseppe Mercurio ◽  
Antonio Scopa
Keyword(s):  
Arundo Donax ◽  
Stem Cutting ◽  
Giant Reed ◽  
Stem Cuttings ◽  
Mother Plants ◽  
Propagation Methods ◽  
One Year ◽  
Arundo Donax L

The increasing interest in<em> Arundo donax,</em> a perennial lignocellulosic species only reproducing by propagation, requires the setup of cheap, simple and reliable techniques. Considering these targets, stem cutting offers considerable advantages. The present investigation aimed to compare: i) plants obtained by different propagation methods (by rhizome and micropropagation mother plants); ii) plants obtained by stem cuttings from basal, central and apical parts of the stem; iii) different planting periods (spring, summer, autumn). The obtained results showed that the number of new shoots from stem buds was: i) higher in the spring and lower in the summer planting period; ii) higher from cuttings obtained by micropropagated than rhizome mother plants, both in spring and summer plantings; iii) decreasing passing from the basal to the apical stem portion; iv) partly unexpressed in the autumn planting period; v) lower from one-year stem cuttings as compared to two-year stem cuttings.

scholarly journals PENGGUNAAN FITOHORMON EKSTRAK BAWANG MERAH (Allium cepa L.) UNTUK PERTUMBUHAN MATERI SETEK BATANG PANGKAL, TENGAH, DAN PUCUK TANAMAN MURBEI (Morus multicaulis)

2019 ◽  
Vol 7 (3) ◽  
Author(s):  
Nengsih Nengsih ◽  
Abdurrani Muin ◽  
Iskandar A M
Keyword(s):  
Plant Growth ◽  
Research And Development ◽  
Experimental Method ◽  
Allium Cepa ◽  
Branch Length ◽  
Stem Cutting ◽  
Stem Cuttings ◽  
Allium Cepa L ◽  
Main Plot ◽  
Plot Design

The research aim to determine the effect of the use of phytohormone extracts of red onion (Allium cepa), stem cuttings material and the interaction of phytohormone extracts of A. cepa with stem cuttings material on the growth of mulberry (Morus multicaulis) plants. This research was conducted at the Research and Development Institute of Tanjungpura University from March to May 2019. The method of research was an experimental method with a Split Plot Design. The treatments consisted of the stem cutting materials (base, middle and shoot) as the main plot and the dose of shallots extract (0 g/l, 500 g/l and 1000 g/l) as subplots. Each treatment consisted of three groups, so that the total of treatments were 27 plots. The results showed that only the dose of shallots extract which significant influented on the M. multicaulis  growth. Whereas the stem cutting materials and interactionsof both were did not effect significantly. The highest of murbei growth was obtained at 1000 g/l of shallot extract on 6,52% of the leaves number, 500 g/l of branch length 4,52% and 3.88% of the roots number. Thus, the extract of A. cepa L can be used as an alternative for plant growth, especially M. multultulis plants.Keywords: Mulberry, stem cuttings, phytohormone.

scholarly journals TRANSPLANTATION OF POTATO SPROUT CUTTINGS ON WET PAPER TO IMPROVE AND INCREASE ROOTING AND SPEED UP MULTIPLICATION BY STEM CUTTING

HortScience ◽  
1994 ◽  
Vol 29 (4) ◽  
pp. 247e-247
Author(s):  
Andrew Frève ◽  
M. Auger
Keyword(s):  
Relative Humidity ◽  
Stem Cutting ◽  
Axillary Buds ◽  
Stem Cuttings ◽  
Damping Off ◽  
Face To Face ◽  
Potato Sprout ◽  
Speed Up ◽  
Bottom Cover ◽  
Paper Towels

Rooting sprout cuttings (spc) is sometimes less successful than stem cuttings (stc). A rooting technique was developed between 1988 and 1991 at La Pocatière. The spc were taken after standing 72 hours in the dark followed by 1 day with 16 hours of light. The spc can be very small (2 mm): a node can be split in half if there are two axillary buds face to face. The spc were soaked in IBA and sandwiched between two paper towels. As many as 300 to 400 spc can be placed in a 28 × 53-cm flat. The papers were placed in flats between two transparent covers; the bottom cover contains water. The paper was moistened with water. The cuttings were maintained at 100% relative humidity for 7 days, then planted in soil in flats. After 5 days on paper, the unrooted cuttings were treated again with IBA on a larger surface and put back in the sandwich. There was less damping-off because the cut part was not placed immediately into the soil. One week after plantation, the plantlets' shape was better and more uniform than those planted directly into soil. The plants rooted on paper produce more stc/spc and more stc/stc than those rooted in soil.

scholarly journals Rooting of semi-hardwood stem cuttings from current year shoots of Drymis brasiliensis

2016 ◽  
Vol 46 (12) ◽  
pp. 2129-2134 ◽  
Author(s):  
Luciele Milani Zem ◽  
Katia Christina Zuffellato-Ribas ◽  
Maria Izabel Radomski ◽  
Henrique Soares Koehler
Keyword(s):  
Experimental Design ◽  
Alcoholic Solution ◽  
Experimental Unit ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Apical Portion ◽  
Randomized Experimental Design ◽  
Cutting Length ◽  
Drimys Brasiliensis ◽  
Mortality Percentage

ABSTRACT: Drimys brasiliensis Miers is an Angiosperm native to the Atlantic Rainforest, commonly known as cataia. Because of dormancy of its seeds, due to embryonic immaturity, production of cataia seedlings presents challenges regarding propagation of the species. Thus, cuttings emerged as a possible technique to be applied, diminishing plants production time and ensuring uniformity of rooting. Stem cuttings from current year shoots were collected in autumn/2012, prepared with 10-12cm in length, a bevel cut on base and straight on top, keeping two leaves, one leaf or no leaves in the apical portion. After disinfestation, bases of cuttings were submitted to the following treatments with indole-3-butyric acid (IBA) in 50% hydro-alcoholic solution: 100% water, 0, 500, 1500, 3000, 4500 and 6000mg L-1 IBA. A completely randomized experimental design was used, with 3 types of cutting x 7 IBA concentrations, with four replicates of 10 cuttings per experimental unit. After 120 days, the variables percentage of rooted cuttings, number of roots per cutting, length of the three longest roots per cutting, percentage of cuttings with callus, alive and dead, with new shoots and the cuttings maintaining the original leaves were assessed. The application of IBA had no influence on any of the assessed variables. Cuttings with two leaves presented the best rooting percentage (51.1%) and the lowest mortality (5.4%), when compared to cuttings with one leaf (35.0%) or without leaves (0.4%). Cuttings without leaves are to be avoided, since they present the highest mortality percentage (93.6%).

scholarly journals Enraizamento de estacas de flor de jade (Strongylodon macrobotrys A. Gray) tratadas com ácido indolbutírico

2015 ◽  
Vol 21 (3) ◽  
pp. 363
Author(s):  
Fabiana Rezende Muniz ◽  
Paulo Hercílio Viegas Rodrigues ◽  
Simone Rodrigues Da Silva
Keyword(s):  
Water Mist ◽  
Stem Cutting ◽  
Stem Cuttings ◽  
Native Plant ◽  
Main Root ◽  
Mist Chamber

The jade vine (Strongylodon macrobotrys A. Gray), is native plant from Philippines. It has long blue-green pseudoracemes inflorescence, which makes it unique and incomparable. It is considered one of the most admired and sought vines, although rare in gardens. This study evaluated the effect of indolbutiric acid (IBA) on the stalks rooting of this ornamental specie. Stem cuttings without leaves and with a couple of leaves cut in half, were collected from branches of a well developed jade vine plant and were immerged into dipping solutions with a concentration range of IBA (0, 500, 1.000, 2.000 and 4.000 mg L-1) for 15 seconds. Subsequently, the bases of the cuttings was planted in polystyrene trays containing vermiculite (one cutting per cell) and maintained in an intermittent water mist chamber for a 80 days period. Then the percentage of stem rooting was assessed as well as the number of roots and the length of the main root. Jade vine plants can be produced byr stem cutting treated in a dipping solution containing an IBA concentration of 2.000 mg L-1.

scholarly journals Peduncle and fruit yield, in six cropping seasons, of early dwarf cashew tree clones irrigated with different water regimes

2004 ◽  
Vol 26 (3) ◽  
pp. 474-477 ◽  
Author(s):  
Kathia Maria Barbosa e Silva ◽  
Francisco Aécio Guedes Almeida ◽  
Paulo Sérgio Lima e Silva
Keyword(s):  
Growth And Development ◽  
Water Regime ◽  
Block Design ◽  
Fruit Yield ◽  
Plant Growth And Development ◽  
Clonal Population ◽  
Random Block ◽  
Random Block Design ◽  
Cropping Seasons ◽  
Cashew Tree

The objective of this work was to evaluate peduncle and fruit yield in clone MS 076 and in a clonal population of drip-irrigated, early dwarf cashew trees propagated by layering, in six cropping seasons. In order to meet the increased water requirements of the crop resulting from plant growth and development, irrigation during the dry season was performed daily according to the following water regime: 15 min/plant/day during the 1st year, 30 min/plant/day during the 2nd year, 45 min/plant/day during the 3rd year and 60 min/plant/day during all subsequent years. Water was supplied by one drip emitter/plant, at an (adjustable) flow rate of 36 L/h.The research was carried out in Fortaleza-Ceará, Brazil, and a random block design was utilized, with five replicates and split-plots. The clones were assigned to plots and the cropping seasons were considered as subplots. The clonal population was superior to the clone only with regard to number of nut shells (NNS), and solely in the first season. The clone was superior to the population as to NNS and peduncle yield (PY) in the second season, and also with regard to the three evaluated traits - NNS, PY, and nut shell yield, in the last three cropping seasons.

scholarly journals Morphological Development of Lingonberry as Affected by In Vitro and Ex Vitro Propagation Methods and Source Propagule

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 760-763 ◽  
Author(s):  
Samir C. Debnath
Keyword(s):  
Vegetative Growth ◽  
Nutrient Medium ◽  
Growth And Development ◽  
Shoot Proliferation ◽  
Morphological Development ◽  
Stem Cuttings ◽  
Ex Vitro ◽  
Propagation Methods ◽  
In Vitro Shoot Proliferation

The growth and development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants and by shoot regeneration from excised leaves of micropropagated shoots, were studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. After 3 years of growth, the in vitro-derived plants produced more stems, leaves, and rhizomes than the conventional cuttings which rarely produced rhizomes. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favor rhizome production. This increase in vegetative growth and rhizome yield of in vitro-derived plants over stem cuttings varied among genotypes.

scholarly journals (261) The Use of Stem Cuttings to Determine Chilling Requirement in Blackberries

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1048D-1048
Author(s):  
P. Manjula Carter ◽  
John R. Clark
Keyword(s):  
Stem Cutting ◽  
Chilling Requirement ◽  
Stem Cuttings ◽  
Chilling Requirements ◽  
Lateral Shoots ◽  
Mist Chamber

Chilling requirement, (the number of hours below 7 °C necessary to break dormancy) has been shown to vary with genotype in blackberry (Rubus subgenus Rubus). Previous work has demonstrated that the chilling requirement of field-grown plants could be accurately determined from stem cuttings of lateral shoots taken at 100-hour intervals of chilling up to 1000 hours, by placing them in a mist chamber maintained at 26 °C with a daylength of 16 hours, and observing budbreak over a period of 5 weeks. This technique has previously demonstrated clear differences in the chilling requirements of thorny and thornless floricane-fruiting cultivars. In the current study, a comparison of floricane-fruiting and primocane-fruiting blackberries using the stem-cutting technique illustrated a lower chilling requirement associated with the primocane-fruiting trait. The use of the stem-cutting technique can be a simple and effective tool for assessing blackberry adaptation to different hardiness zones.

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