scholarly journals THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENS

2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Aditya Pramanta ◽  
Johan Renaldo ◽  
Doddy M Soebadi
Keyword(s):  
Sperm Motility ◽  
Vas Deferens ◽  
Sperm Quality ◽  
Wistar Rat ◽  
Cloning And Expression ◽  
Absorbable Suture ◽  
Recombinant Erythropoietin ◽  
Sperm Retrieval ◽  
Glycoprotein Hormone ◽  
Vasectomy Reversal

Objective: The patency rates after vasectomy reversal ranges from 71-97%, but there is 26-72% possibility of persistent infertility. Dysfunction or obstruction of the epididymis and oxidative stress are thought to be the important cause of male infertility by disrupting spermatozoa maturation process, causing poor sperm quality. Human erythropoietin or better known as EPO is a glycoprotein hormone that has been purified since three decades ago. Research on the EPO has evolved and become a major research topic the researchers aimed as a therapeutic agent. The cloning and expression of erythropoietin has developed recombinant erythropoietin as a drug that serves as an anti-oxidant, anti-apoptotic and anti-inflammatory. This study aimed to determine the effect of erythropoietin supplementation on sperm motility and morphology in Wistar rat after the release of the vas deferens’ ligation. Material & Methods: Twenty four male Wistar rats were randomly divided into four groups (6 each). On the vasectomy group, the vas deferens were serially ligated for 7 weeks using a non-absorbable suture. The vasectomy reversal group get the same surgical treatment and after 7 weeks the ligation were released. While as in the erythropoietin group, recombinant eryhtropoietin (1000 IU/kg) was administered intraperitoneally three times for 1 week after releasing the ligation. Normal control animals received no surgical manipulation and followed by sperm retrieval for analysis. Eosin-stained slides were prepared to assess the motility and morphology of sperm cells and observed under a light microscope. Results: Ligation of vas deferens significantly decreased sperm motility and morphology. Releasing the ligation of the vas deferens did not improve the sperm motility and morphology. Supplementing eryhtropoietin 1000 IU/kg 3 times for a week after releasing the ligation did not improve the sperm motility and morphology. Conclusion: Erythropoietin supplementation did not improve the sperm motility and morphology in Wistar rat after releasing the ligation of vas deferens.

scholarly journals Localization of sperm intracellular Ca2+ keeps fertilizability in the newt vas deferens

Reproduction ◽  
2020 ◽  
Vol 159 (3) ◽  
pp. 339-349
Author(s):  
Nanae Makino ◽  
Nozomi Sato ◽  
Eriko Takayama-Watanabe ◽  
Akihiko Watanabe
Keyword(s):  
Sperm Motility ◽  
Salt Solution ◽  
Acrosome Reaction ◽  
Vas Deferens ◽  
Sperm Quality ◽  
Ethylene Glycol Tetraacetic Acid ◽  
Channel Blockers ◽  
Tetraacetic Acid ◽  
Intracellular Ca ◽  
Principal Piece

Sperm intracellular Ca2+ is crucial for the induction of sperm-egg interaction, but little is known about the significance of Ca2+ maintenance prior to induction. In sperm of the newt Cynops pyrrhogaster, intracellular Ca2+ is localized to the midpiece during storage in the vas deferens, while extracellular Ca2+ is influxed in modified Steinberg’s salt solution to promote a spontaneous acrosome reaction related to the decline of sperm quality. In the present study, sperm from the vas deferens were loaded with the Ca2+ indicator Fluo8H, and changes in Ca2+ localization in modified Steinberg’s salt solution were examined. Calcium ions expanded from the cytoplasmic area of the midpiece to the entire tail in most sperm during a 1-h incubation and localized to the principal piece in some sperm within 24 h. Similar changes in Ca2+ localization were observed in reconstructed vas deferens solution that included ions and pH at equivalent levels to those in the vas deferens fluid. Sperm with Ca2+ localization in the entire tail or the principal piece weakened or lost responsiveness to sperm motility-initiating substances, which trigger sperm motility for fertilization, but responded to a trigger for acrosome reaction. The change in Ca2+ localization was delayed and transiently reversed by ethylene glycol tetraacetic acid or a mixture of Ca2+ channel blockers including Ni2+ and diltiazem. These results suggest that C. pyrrhogaster sperm localize intracellular Ca2+ to the midpiece through Ca2+ transport in the vas deferens to allow for responses to sperm motility-initiating substances.

Successful pregnancy in double factor infertility: Sperm in vitro culture by modified testicular fine-needle aspiration

2019 ◽  
Vol 86 (3) ◽  
pp. 141-144
Author(s):  
Gianmartin Cito ◽  
Maria Elisabetta Coccia ◽  
Francesco Bertocci ◽  
Rita Picone ◽  
Andrea Cocci ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Sperm Motility ◽  
Fine Needle Aspiration ◽  
Intracytoplasmic Sperm Injection ◽  
Vas Deferens ◽  
Needle Aspiration ◽  
Testicular Sperm ◽  
Sperm Retrieval ◽  
Fine Needle

Introduction: Infertility may depend up to 27% of couples on both partners. In patients with obstructive azoospermia, testicular fine-needle aspiration represents a good option to retrieve spermatozoa, in order to perform an assisted reproductive treatment. In vitro maturation of testicular spermatozoa could be the better choice of treatment in view of the increased motility, improving fertilization and pregnancy rates. Case description: A 34-year-old azoospermic man and his 33-year-old partner referred for treatment of simultaneous male and female infertility factor. The woman presented a diminished ovarian reserve, with serum follicle stimulating hormone value of 27.15 IU/L. The man underwent trans-rectal and testicular ultrasounds that detected the congenital absence of proximal vas deferens on the right side and the absence of seminal vesicle and distal vas deferens on the left side. We proposed a chance to have their own biological child. The man underwent modified testicular fine-needle aspiration using a 18-gauge butterfly needle. Sperm retrieval was successful with 0.001 × 106 spermatozoa/mL and absence of motility. Testicular sperm suspension was cultured for 24 h to identify sperm viability, achieving 10% of sperm motility. Two metaphase II oocytes were retrieved and processed with intracytoplasmic sperm injection. Clinical pregnancy with live birth was obtained. Conclusion: Performing modified testicular fine-needle aspiration increases successful sperm retrieval. Testicular sperm in vitro culture for 24 h proved to be a real and practical technique to increase sperm motility, in order to select mature and viable spermatozoa and improve successful intracytoplasmic sperm injection outcomes.

scholarly journals CagA-PositiveHelicobacter pyloriInfection and Reduced Sperm Motility, Vitality, and Normal Morphology

2013 ◽  
Vol 35 ◽  
pp. 229-234 ◽  
Author(s):  
E. Moretti ◽  
G. Collodel ◽  
L. Mazzi ◽  
M. S. Campagna ◽  
N. Figura
Keyword(s):  
Sperm Motility ◽  
Normal Forms ◽  
Molecular Mimicry ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Normal Morphology ◽  
Partial Linear ◽  
Caga Status ◽  
The Relationship ◽  
Autoimmune Phenomena

Helicobacter pylori(HP) infection, particularly when caused by strains expressing CagA, may be considered a concomitant cause of male and female reduced fertility. This study explored, in 87 HP-infected males, the relationship between infection by CagA-positive HP strains and sperm parameters. HP infection and CagA status were determined by ELISA and Western blotting; semen analysis was performed following WHO guidelines. The amino acid sequence of human enzymes involved in glycolysis and oxidative metabolism were “blasted” with peptides expressed by HP J99. Thirty-seven patients (42.5%) were seropositive for CagA. Sperm motility (18% versus 32%; ), sperm vitality (35% versus 48%; ) and the percentage of sperm with normal forms (18% versus 22%; ) in the CagA-positive group were significantly reduced versus those in the CagA-negative group. All the considered enzymes showed partial linear homology with HP peptides, but four enzymes aligned with four different segments of the samecagisland protein. We hypothesize a relationship between infection by strains expressing CagA and decreased sperm quality. Potentially increased systemic levels of inflammatory cytokines that occur in infection by CagA-positive strains and autoimmune phenomena that involve molecular mimicry could explain the pathogenetic mechanism of alterations observed.

scholarly journals Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

2015 ◽  
Vol 75 (3) ◽  
pp. 662-669 ◽  
Author(s):  
EG Sanches ◽  
IR Oliveira ◽  
PCS Serralheiro ◽  
VR Cerqueira
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Equilibration Time ◽  
Dilution Ratio ◽  
Sperm Cryopreservation ◽  
Ph Values ◽  
Cryopreserved Sperm ◽  
Time Density ◽  
Motility Rate

AbstractThis study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C –min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C –min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.

scholarly journals Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

2017 ◽  
Vol 38 (5) ◽  
pp. 3069 ◽  
Author(s):  
Ana Carolina Rodrigues ◽  
Camila Montanari Ruiz ◽  
Carla Daniela Dan De Nardo ◽  
Gabriele Barros Mothé ◽  
Fabiano Martinez Rossi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Dietary Supplementation ◽  
Reproductive Efficiency ◽  
Omega 3 ◽  
Oral Supplementation ◽  
Animal Reproduction ◽  
Fresh Semen

For years, fatty acids have been recommended as a dietary supplement to improve canine hair. For animal reproduction, supplementation with omegas has been used to increase the reproductive efficiency and conception rate, but few studies have been conducted in dogs. The aim of this study was to evaluate the effects of daily dietary supplementation with omega-3 and -6 on the quality of fresh and frozen/thawed semen in canines. Semen was collected from seven dogs and evaluated for sperm motility, vigor, concentration, and morphology. The 17-week study included 119 ejaculates and was divided according to oral supplementation with omega-3 and -6: M1 (1st-5th week) or pre-supplementation; M2 (6th-9th week) and M3 (10th-13th week) or during supplementation; and M4 (14th-17th week) or post-supplementation. After analysis, the semen was frozen and then revaluated both immediately and 30 minutes (at 37° C) after thawing. Supplementation with omegas increased sperm motility, vigor, and concentration; however, supplementation had no influence on semen freezability. In addition, there was no improvement in sperm motility after supplementation when the thawed cells were maintained at 37° C for 30 minutes. We concluded that dietary supplementation with omega-3 and -6 for 4 to 8 weeks can improve the quality of fresh semen, although it has no effect on the freezability of canine semen.

scholarly journals A Rare Variation of Transverse Testicular Ectopia (TTE) in a Young Adult as an Incidental Finding during Investigation for Testicular Pain

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
Chrysovalantis Gkekas ◽  
Evangelos N. Symeonidis ◽  
Ioannis Tsifountoudis ◽  
Christos Georgiadis ◽  
Vasileios Kalyvas ◽  
...  
Keyword(s):  
Vas Deferens ◽  
Sperm Count ◽  
Incidental Finding ◽  
Sperm Retrieval ◽  
Testicular Pain ◽  
Transverse Testicular Ectopia ◽  
Ectopic Testis ◽  
Close Proximity ◽  
Magnetic Resonance Imaging Mri ◽  
Testicular Ectopia

Transverse testicular ectopia (TTE) with fused vas deferens is an extremely rare clinical entity. Herein, we present a case of a 19-year-old patient with persistent left testicular pain lasting for a week. Clinical examination revealed an empty right hemiscrotum, a normal left-sided descended testis, and in close proximity a mass-like structure resembling testicular parenchyma. Laboratory tests were significant for elevated follicle-stimulating hormone (FSH), while sperm count revealed azoospermia. Ultrasound imaging (US) of the scrotum demonstrated the presence of both testes in the same left hemiscrotum with varicocele and no signs of inguinal hernia. Magnetic resonance imaging (MRI) of the penis and scrotum revealed TTE with a single, fused vas deferens, and hypoplastic seminal vesicles. Surgical intervention by means of microsurgical sperm retrieval and transseptal orchidopexy were considered but not performed, primarily owing to the patient’s unwillingness and to a lesser extent due to the restriction that the short and fused vas would pose in an attempt to transpose the ectopic testis. Therefore, an annual follow-up was recommended.

Effect of storage for 24 h at 18°C on sperm quality and a comparison of two assays for sperm membrane lipid peroxidation

2010 ◽  
Vol 90 (3) ◽  
pp. 389-392 ◽  
Author(s):  
N. Am-in ◽  
R N Kirkwood ◽  
M. Techakumphu ◽  
W. Tantasuparuk
Keyword(s):  
Lipid Peroxidation ◽  
Membrane Permeability ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Membrane Lipid ◽  
Membrane Lipid Peroxidation ◽  
Group A ◽  
Sperm Membrane ◽  
Fresh Semen

Boars having normal (71.1 ± 1.2%; n = 10) or low (35.12 &plusmn 3.9%; n = 10) sperm motility 24 h after collection were used, and semen was evaluated following storage in Beltsville Thawing Solution (BTS) for 24 h at 18°C. Sperm lipids were extracted and lipid peroxidation quantified. No differences were evident in fresh semen, but after 24 h, sperm motility, viability and membrane permeability in the low motility group were lower (P < 0.001) compared with the normal motility group. Sperm membrane lipid peroxidation was greater (P < 0.001) in the low motility group. A factor influencing sperm storability is membrane lipid peroxidation, which can be accurately assayed using a commercial kit.Key words: Boars, sperm motility, sperm quality, lipid peroxidation

scholarly journals Use of a sperm analyzer for evaluating broiler breeder males. 1. Effects of altering sperm quality and quantity on the sperm motility index

1998 ◽  
Vol 77 (6) ◽  
pp. 888-893 ◽  
Author(s):  
CD McDaniel ◽  
JL Hannah ◽  
HM Parker ◽  
TW Smith ◽  
CD Schultz ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Motility Index ◽  
Broiler Breeder ◽  
Broiler Breeder Males
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