scholarly journals Evaluation of larvae production and growth of juvenile red tilapia NIFI F1 during the nursery phase

2019 ◽  
Vol 18 (3) ◽  
pp. 251
Author(s):  
Adam Robisalmi ◽  
Priadi Setyawan ◽  
Bambang Gunadi

Genetic quality improvement can be done through breeding programs. The indicator of success in the selection is the improvement of the character of production. Larval production and growth of NIFI of F1generation red tilapia seeds in the selection and its control parents were evaluated in this study. The research was conducted at the Fish Breeding Research Center for four months. Spawning was carried out on freshwater ponds of cage with size of  1 x 1 m. Tilapia brood stock were stocked with male to female ratio of 1: 1. The larvae rearing were conducted in the cage with size 2x2x1 m3 and stocking density 125 m-2. Pellets with a protein content of 38-40% are given to ad libitum larvae three times a day for first month, and then feeding rate was given 15-20% daily from biomass. Seed production, growth, specific growth rate, and survival rate were observed. The results of the experiment show a difference between fish selected and control. Seed production of selection reached 540 ± 114 and controls   508 ± 142. The growth performance of selected red tilapia juvenile showed higher compared with the control. The mean length  of  selected population  reached 6.33 ± 0.43 cm , weight gain 6.60 ± 0.52 g, specific growth rate 7.33 ± 0% g days-1 and survival rate  81.50 ± 4.46% were respectively and the control population  5.76 ± 0.52 cm ; 4,90 ± 0.58 g; 4.50 ± 0.35% g days-1; and 74.85 ± 3.26%. These values indicate that the growth of red tilapia juvenile NIFI F1 generation was increased 25.76%.AbstrakSalah satu upaya yang dapat dilakukan untuk meningkatkan kualitas genetik yaitu melalui program pemuliaan. Per-baikan karakter reproduksi dan pertumbuhan dapat digunakan sebagai indikator keberhasilan seleksi. Penelitian ini bertujuan untuk mengevaluasi produksi larva dan pertumbuhan benih ikan nila merah NIFI F1 dari induk seleksi dan kontrol. Kegiatan dilaksanakan selama empat bulan di Balai Riset Pemuliaan Ikan. Pemijahan dilakukan di kolam air tawar pada hapa 1 m x1 m.  Perbandingan induk ikan nila jantan dan betina 1:1. Larva yang sudah dipanen dimasukkan pada hapa pendederan ukuran 2x2x1 m3 dengan padat tebar 125 ekor m-2 . Selama pendederan , larva diberi pakan (protein 38-42%) secara ad libitum tiga kali sehari pada bulan pertama dan selanjutnya diberi pakan dua kali sehari 15-20% dari biomassa. Pakan yang diberikan selama pendederan mempunyai protein berkisar 38-42%. Parameter yang diamati meliputi produksi larva, pertumbuhan, laju pertumbuhan spesifik, dan sintasan. Hasil penelitian menunjukkan produksi larva ikan nila merah seleksi yaitu 540±114 ekor dan kontrol 508±142 ekor. Performa pertumbuhan benih ikan nila merah seleksi menunjukkan hasil yang lebih tinggi dibanding kontrol dengan nilai pertumbuhan panjang 6,33 ±0,43cm, pertumbuhan bobot 6,60±0,52 g, laju pertumbuhan spesifik 7,33±0% g hari-1 serta sintasan 81,50±4,46%, se-dangkan populasi kontrol masing-masing 5,76±0,52 cm; 4,90±0,58 g; 4,50±0,35% g hari-1; dan 74,85±3,26%. Nilai ini menunjukkan bahwa ada peningkatan pertumbuhan sebesar 25,76% pada benih ikan nila merah NIFI F1.

2018 ◽  
Vol 47 ◽  
pp. 02007
Author(s):  
Lies Setijaningsih ◽  
Dewi Puspaningsih ◽  
Imam Taufik

Fulfill needs of Siamese gourami still rely on the catch from nature, so the population in nature has become decreased. The increase of Siamese gourami fish production from culture is still constrained by the survival rate and slow growth rate in the seed phase. The purpose of this research was to determine the effect of Siamese gourami stocking density on the application of optimum temperature and salinity to growth performance for optimization of production capacity. Application of optimum temperature and salinity refers to the results of previous research, i.e., temperature of 28 °C and salinity 3 ‰. Siamese gourami seed with length of 2.96 ± 0.42 cm and weight of 5.27 ± 0.10 g used in the research. The experimental design used completely randomized design with four treatments, i.e. 1 fish L-1, 2 fish L-1, 3 fish L-1 and 4 fish L-1. The results showed that there were an effect of different stocking density on absolute growth weight and length, survival rate, specific growth rate and productivity. This is shown in the C treatment which growth was 4.95 ± 0.037 g, length 3.51 ± 0.01 cm, survival rate 93.47%, specific growth rate 1.22% and fish productivity of 625.76 g.


2021 ◽  
Vol 16 (1) ◽  
pp. 7
Author(s):  
Jojo Subagja ◽  
Otong Zenal Arifin ◽  
Kurniawan Kurniawan ◽  
Vitas Atmadi Prakoso

Ikan semah (Tor douronensis) termasuk salah satu spesies dari genus Tor yang memiliki nilai ekonomis tinggi. Untuk meningkatkan produktivitas, informasi pertumbuhan benih yang dipelihara pada sistem budidaya karamba jaring apung (KJA) perlu dilakukan karena ikan semah umumnya dibudidayakan pada kolam dengan air yang jernih. Penelitian ini bertujuan untuk mendapatkan informasi laju pertumbuhan benih ikan semah generasi pertama dengan perlakuan padat tebar yang berbeda pada sistem pemeliharaan KJA. Percobaan dilakukan di Waduk Cirata, Cianjur, Jawa Barat selama lima bulan. Ikan uji (panjang total: 7-8 cm; bobot: 5-6 g) dipelihara di KJA (padat tebar 50, 75, dan 100 ekor/m3 dengan tiga kali ulangan). Pakan apung komersial dengan kadar protein 40% diberikan sebesar 6% per biomassa yang diberikan dua kali sehari. Parameter yang diamati yaitu pertumbuhan bobot mutlak, sintasan, laju pertumbuhan spesifik (SGR), dan biomassa. Hasil percobaan menunjukkan bahwa perlakuan padat tebar 50 ekor/m3 menghasilkan pertumbuhan terbaik dengan bobot akhir 15,89 ± 1,43 g; pertambahan mutlak 10,48 ± 1,41 g; sintasan 94,67 ± 4,6%; dan SGR 0,75 ± 0,04%. Dari hasil penelitian ini, padat tebar 50 ekor/m3 merupakan kondisi yang terbaik untuk pemeliharaan benih ikan semah di KJA. Hasil penelitian ini dapat dijadikan informasi awal tentang potensi budidaya ikan semah dengan sistem budidaya KJA perlu dikembangkan di masa yang akan datang.Semah mahseer (Tor douronensis) is a species from the genus Tor and highly valued in local and national markets. Nevertheless, the fish production from net cage is currently limited. In order to increase its productivity, research on the growth of semah mahseer fingerlings reared in floating net cage systems needs to be carried out as this species is predominantly cultured in the clean water ponds. This study was conducted to obtain information on the growth performance of the first generation of semah mahseer fingerlings reared with different stocking densities in a floating net cage system. The experiment was carried out in the floating net cage located at Cirata Reservoir, Cianjur, West Java, for five months. Fingerlings (total length: 7-8 cm; body weight: 5-6 g) were reared in the floating net cage (stocking density of 50, 75, and 100 ind./m3 with three replications). The fish were fed with a commercial diet (protein content of 40%) given at 6% of biomass twice a day. The parameters observed included weight gain, survival rate, specific growth rate (SGR), and biomass. The results showed that the stocking density of 50 ind./m3 had the best performance in terms of final weight of 15.89 ± 1.43 g, weight gain of 10.48 ± 1.41 g, survival rate of 94.67 ± 4.6%, and specific growth rate of 0.75 ± 0.04%. Based to the results of this study, the stocking density of 50 ind./m3 is the best condition for rearing semah mahseer fingerlings in floating net cages. The findings of this research can be used as the basic information for future aquaculture development of semah mahseer in net cages.


2021 ◽  
Vol 83 (5) ◽  
pp. 85-92
Author(s):  
Ku Boon Hong ◽  
Musfiroh Jani ◽  
Rabiatul Adawiyah Meor Mohamad Zain ◽  
Norashikin Mohd Fauzi

Food demand is expected to surge due to the increase of world population. Malaysia is one of the top fish consumption countries in Asia, but the reduction of capture fishery has led to the increase for aquaculture for tilapia to overcome the demand. Thus, the objective of this study was to compare the growth performance of red tilapia at different stocking densities of zeolite supplemented closed system. Red tilapia fry was stocked into aquarium. Five treatments (containing zeolite and without zeolite) with two replicates were used. Food was given twice a day with 2% of total biomass of the fish. Weight and length of red tilapia were measured every two weeks. The zeolite treatments which recorded the highest final mean weight was T1 with the lowest stocking density (5 fish) while in T5 (highest stocking density), lowest mean weight was recorded. Specific growth rate and length gain in T1 was significantly higher than other treatments. On the other hand, there was no significant difference in growth parameters between the treatments with and without zeolite. The results revealed that red tilapia stocked in the T1 of the lowest stocking density (5 fish) had the highest growth performance than the fish in other treatments. Compared to other treatments, T1 recorded the highest final length and weight, weight and length gain, Specific Growth Rate % (SGR) among the treatments. The findings will be useful for the practitioners to understand the best practice for stocking density in zeolite supplemented closed system.


2020 ◽  
Vol 7 (1) ◽  
pp. 40
Author(s):  
Anis Zubaidah ◽  
Sri Samsundari ◽  
Yudi Arifraeka Insan

Permintaan pasar yang tinggi pada ikan manfish sangat sulit terpenuhi dikarenakan pertumbuhan ikan manfish yang lambat. Solusi untuk memperbaiki pertumbuhan ikan manfish yaitu mengetahui padat tebar yang baik untuk ikan manfish serta ditambah penerapan teknologi resirkulasi yang di harapkan pertumbuhan ikan menjadi lebih cepat. Berdasarkan permasalahan itu maka dilakukannya penelitian ini untuk melihat respon ikan terhadap pertumbuhan. Metode yang digunakan dalam penelitian ini adalah metode Rancangan Acak Lengkap. Penelitian ini menggunakan 4 perlakuan (P1 (3 ekor/L dengan 25 liter air yaitu 75 ekor/wadah), P2 (6 ekor/L dengan 25 liter air yaitu 150 ekor/wadah), P3 (9 ekor/L dengan 25 liter air yaitu 225 ekor/wadah), dan P4 (12 ekor/L dengan 25 liter air yaitu 300 ekor/wadah) yang masing-masing perlakuan diulang sebanyak 3 kali ulangan. Hasil penelitian menunjukkan bahwa nilai rerata SGR (specific growth rate) yang terbaik adalah pada pelakuan P1 dengan pelakuan 3 ekor/L dengan air 25 liter yaitu 75 ekor/wadah, dengan rerata SGR sebesar 5,12 %. Laju pertumbuhan panjang yang terbaik adalah pada pelakuan P1 dengan pelakuan 3 ekor/L dengan air 25 liter yaitu 75 ekor/wadah, dengan rerata laju pertumbuhan panjang sebesar 1,40 %. Kelangsungan hidup (survival rate) terbesar adalah pada perlakuan P1 dengan pelakuan 3 ekor/L dengan air 25 liter yaitu 75 ekor/wadah, dengan rerata kelangsungan hidup (survival rate) sebesar 86,22%. Secara statistik pada penelitian ini mendapatkan hasil berpengaruh nyata terdapat pertumbuhan dan kelulusan hidup.Kata kunci: ikan manfish; resirkulasi; petumbuhan; kelulusan hidup High market demand for manfish is challenging to fulfill due to the slow growth of manfish. The solution to improve the growth of manfish is knowing good stocking density for manfish and adding the application of recirculation technology, which is expected to accelerate fish growth. Based on these problems, this study was conducted to see the response of fish to growth. The method used in this study is a completely randomized design method. In this study, we were using four treatments (P0 (3 fish/L with 25 liters of water is 75 fish/aquarium), P1 (6 fish/L with 25 liters of water is 150 fish/aquarium), P2 (9 fish /L with 25 liters water which is 225 fish/aquarium), and P3 (12 fish/L) with 25 liters of water, i.e., 300 fish/aquarium), each of which is repeated three times. The results show that the best SGR (specific growth rate) was in the treatment P1 with the treatment of 3 fish/L with 25 liters of water, which is 75 fish/aquarium, with a mean SGR 5.12%. The best high growth rate was at P1 treatment with three fish/L treatment with 25 liters of water, which is 75 fish/aquarium, with a high growth rate 1.40%. The most significant survival rate was in treatment P1 with the treatment of 3 fish/L with 25 liters of water, which is 75 fish/aquarium, with a mean survival rate amounting 86.22%, statistically in this study getting influential results or there were growth and survival rate.Keywords: manfish; recirculation; growth; survival rate


Author(s):  
Dio Patria Yustysi ◽  
Fajar Basuki ◽  
Titik Susilowati ◽  
Tristiana Yuniarti

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh dari hibridisasi ikan nila Pandu F6 dengan nila Nilasa terhadap karakter reproduksinya dan performa benih yang dihasilkan. Penelitian ini dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini: perlakuan A (Pandu F6 ♀><Pandu F6 ♂), B (Nilasa ♀><Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), dan D (Nilasa ♀><Pandu F6 ♂). Data yang diamati meliputi fekunditas, daya tetas telur, diameter dan bobot telur, panjang dan bobot larva TL, panjang dan bobot larva lepas kuning telur, kelulushidupan, laju pertumbuhan spesifik, konversi pakan dan kualitas air. Hasil penelitian menunjukan nilai terbaik pada fekunditas sebesar 1191,67 ± 239,45 butir/200 g, daya tetas telur sebesar 80,93 ± 3,90%, kelulushidupan sebesar 81,55 ± 7,54%, laju pertumbuhan spesifik sebesar 7,26 ± 0,25, konversi pakan 0,54 ± 0,02 didapatkan pada perlakuan C, akan tetapi untuk diameter dan bobot telur, panjang dan bobot larva Kuning Telur, dan panjang dan bobot larva lepas kuning telur tidak berbeda nyata. Hasil tersebut menunjukan bahwa hibridisasi (perlakuan C) memberikan pengaruh yang nyata (P < 0,05) terhadap fekunditas, daya tetas telur, kelulushidupan, laju pertumbuhan spesifik, dan konversi pakan tetapi tidak berpengaruh terhadap diameter dan bobot telur, panjang dan bobot larva, dan panjang dan bobot larva lepas kuning telur. Kata kunci: Hibridisasi, Ikan Nila, Karakter Reproduksi, Performa Benih  ABSTRACT This research aims to know the influence of hybridization tilapia fish Pandu F6 with nila Nilasa against the character of the reproduction and seed performance. This research was conducted with the experimental method using Random Design complete (RAL) with 4 treatments and three replicates. The treatments in this study: the treatment A (Pandu F6 F6 ♀ >< Pandu ♂), B (Nilasa ♀>< Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), and D (Nilasa ♀ >< Pandu F6 ♂). The observed data covering fecundity, hatching rate, egg size, yolk sack larva length and weight, length and weight of the egg yolk off larvae, survival rate, specific growth rate, feed conversion rate, and water quality. The results showed the best value on the fecundity of 1191.67±239.45 eggs/200 g, Hatching rate 80.93 ± 3.90%, Survival rate of 81.55 ± 7.54%, specific growth rate of 7.26 ± 0.25, conversion fodder 0.54 ± 0.02 obtained at the treatment C, but for the diameter and weight of egg, larval length and weight TL, and the length and weight of the yolk egg off larvae for each treatment do not differ markedly. The results showed that hybridization (treatment C) gives a real influence (P < 0.05) of fecundity,hatching rate, survival rate but not with the egg size, larva weight and length, and the length and weight of larvae off yolk. Water quality on the spawning, hatching eggs and larvae found on the maintenance of a decent range for tilapia fish farming Keywords: hybridization, tilapia fish, character of the reproduction, seed performance 


2021 ◽  
Vol 7 (2) ◽  
pp. 1-6
Author(s):  
Numisye Iske Mose ◽  
Jetti Treslah Saselah

Tanaman wori merupakan salah satu tanaman kelompok fabaceace yang tumbuh melimpah di Sangihe dan sangat potensial digunakan sebagai salah satu sumber pakan ikan. Kadar protein daun wori adalah 25,53% sehingga berpotensi menjadi protein nabati bagi ikan. Kendala utama yang ditemui ketika memanfaatkan daun tanaman wori adalah kandungan karbohidrat yang tinggi yaitu 48,51% sehingga dapat mempengaruhi proses pencernaan ikan, penyerapan nutrisi yang berujung pada rendahnya pertumbuhan ikan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung daun wori femerntasi terhadap laju pertumbuhan spesifik dan sintasan hidup ikan nila. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL). Perlakuan yang dilakukan terdiri dari tiga perlakuan dan tiga kali ulangan yaitu kadar tepung daun wori tanpa fermentasi A (kontrol) 10%, kemudian B tepung daun wori fermentasi (10%), dan C tepung daun wori fermentasi (20%). Laju pertumbuhan spesifik dan efisiensi pakan diuji menggunakan ANOVA sedangkan sintasan hidup akan dianalisis secara deskriptif. Hasil penelitian ini menunjukan bahwa penambahan tepung wori fermenasi dalam pakan dapat meningkatkan nilai laju pertumbuhan spesfik ikan nila dan efisiensi pemanfaatan pakan dengan nilai tertinggi pada perlakuan C yaitu SGR 1,94%/hari. Sementara itu, untuk semua perlakuan nilai sintasan hidup sebesar 100%.   The wori plant is one of the plants of the fabaceace group that grows abundantly in Sangihe and has potential to be used as a source of fish feed. The protein content of wori leaves is 25.53%, so it has the potential to become vegetable protein for fish. The main obstacle encountered when utilizing wori plant leaves is the high carbohydrate content of 48.51% so that it can affect the digestion process of fish, nutrient absorption which lead to low fish growth. The purpose of this study was to determine the effect of wori femerntation leaf meal on the specific growth rate and survival of tilapia. The experimental design used was a completely randomized design (CRD). The treatments consisted of three treatments and three replications, namely the content of fermented wori leaf powder, A (control) 10%, then B fermented wori leaf powder (10%), and C fermented wori leaf powder (20%). Specific growth rate and feed efficiency were tested using ANOVA, while survival rate was analyzed descriptively. The results of this study indicate that the addition of fermented wori powder in the feed can increase the value of the specific growth rate of tilapia and feed utilization efficiency with the highest value in treatment C, namely SGR 1.94% / day. Meanwhile, for all treatments the survival rate was 100%.


2017 ◽  
Vol 16 (1) ◽  
pp. 60
Author(s):  
, Purnamawati ◽  
Daniel Djokosetiyanto ◽  
Kukuh Nirmala ◽  
Enang Harris ◽  
Ridwan Affandi

<p><strong>ABSTRACT </strong></p><p> </p><p>The aim of the research was to analyze survival rate, specific growth rate, albumin, and feed efficiency and physiological (blood glucose, cortisol, dan haemoglobin) responses of snake head fish juvenil that reared at aerated and unaerated of tidal land water have been conducted in the laboratory. Experiments using completely randomized design with aerated and unaerated as a treatment, and each treatment has twelve replications. The snakehead fish juvenil wich length 2.4±0.2 cm and weight of 0.21±0.05 g reared in the aquarium that are size 30×25×35 cm (water volume 25 L) with a stocking density 2 juvenile/L, for 40 days. The fishes were fed with commercial feed with protein content about 40%, feeding two times a day (morning and afternoon) were at satiation. Replacement of water done every two days about 10% of the total water volume in the aquarium. The results showed that unaerated median significantly affected to biometric and physiological response of juvenile of snake head fish. The media un-aerated gives the best results shown by the higher value of survival (92%), specific growth rate (6.73%/ day), feed efficiency (78.22%), protein retention (41.91%), energy retention (30.81%) value of albumin (6.60 g/100 mL) and the haemoglobin (5.58 g/dL), and have the lowest value of cortisol (21.49 ng/L) and blood glucose (43.36 mg/100 mL).</p><p> </p><p>Keywords: acid sulfate water, growth rate, aeration, <em>Channa striata </em></p><p><em> </em></p><p> </p><p><strong>ABSTRAK </strong></p><p> </p><p>Penelitian ini bertujuan untuk menganalisis respons kelangsungan hidup, pertumbuhan spesifik, albumin, dan efisiensi pakan dan fisiologis (kortisol, glukosa darah, dan hemoglobin) juvenil ikan gabus yang dipelihara dengan dan tanpa aerasi pada media air rawa pasang surut. Penelitian menggunakan rancangan acak lengkap dengan aerasi dan tanpa aerasi sebagai perlakuan, dan masing-masing perlakuan memiliki 12 ulangan. Juvenil ikan gabus berukuran panjang 2,4±0,3 cm dan bobot 0,21±0,03 g dipelihara dalam akuarium 30×25×35 cm (volume 25 L) dengan padat tebar 2 ekor/L, selama 40 hari. Ikan diberi pakan berupa pakan komersial dengan kadar protein ±40%, pemberian pakan dua kali sehari (pagi dan sore) <em>at satiation</em>. Penggantian air dan penyiponan dilakukan dua hari sekali sebanyak 10% dari volume total dalam akuarium. Hasil penelitian menunjukkan bahwa perlakuan tanpa aerasi berpengaruh nyata terhadap respons biometrik dan fisiologis juvenil ikan gabus. Media tanpa aerasi memberikan hasil yang lebih baik ditunjukkan oleh kelangsungan hidup (92%), laju pertumbuhan spesifik (6,73%/ hari), efisiensi pakan (78,22%), retensi protein (41,91%), retensi energi (30,81%), kadar albumin (6,60 g/100 mL), dan hemoglobin (5,85 g/dL) yang lebih tinggi, sedangkan kadar kortisol (219 ng/L) dan glukosa darah (43,36 mg/100 mL) yang terendah.</p><p> </p><p>Kata kunci: media sulfat masam, pertumbuhan, aerasi, <em>Channa striata</em></p>


2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Ayu Herdianti Primashita ◽  
Boedi Setya Rahardja ◽  
Prayogo .

Catfish (Clarias sp.) is a freshwater fish that is widely cultivated because it is a leading commodity. Increased production of catfish resulted in extra area of cultivated land and water use. The technology suitable to be applied is aquaponic system. In addition to saving land and water use, Aquaponic is also improving business efficiency through the utilization of nutrients from food remains and fish metabolism. Water quality is an important factor for the success of cultivation. Maintenance catfish with the addition of probiotics in aquaponic system can be a solution for maintaining water quality, because it contains bacteria that can increase nitrite to nitrate changes that can be utilized for growth of plants and do not poison the fish. The purpose of this study was to determine the effect of different probiotics in the aquaponic system towards growth rate and survival rate of catfish. The method that used in this research is experimental with completely randomized design (four  treat and five repeated) are P0 (control), P1 (probiotic A), P2 (probiotic B) and P3 (probiotic C). Analysis of data processed using Analysis of Variance. If there are significant differences then continued Duncan's Multiple Range Test. The results showed that the giving of probiotics in the aquaponic system is significantly different (p <0.05). The final conclusion is that the addition of probiotics in  aquaponic system affect the growth rate and survival rate of catfish. The highest specific growth rate (0.025%/ day) and the highest survival rate (77.8%) contained in the P2 treatment. The lowest specific growth rate (0.019% /day) and the lowest survival rate (64.4%) contained in P0 treatment (control). Keywords : Aquaponic, Catfish, Probiotic, Growth Rate, Survival Rate, Clarias sp.


Author(s):  
Rachmat Nurul Huda ◽  
Titik Sulistiowati ◽  
Tristiana Yuniarti

<p align="center"><strong>ABSTRAK</strong></p><p>Budidaya ikan nila <em>(Oreochromis niloticus) </em>terus dikembangkan hingga saat ini. Berbagai teknologi dalam bidang perikanan telah diterapkan, salah satunya yaitu <em>sex reversal </em>dalam hal ini jantanisasi. Ikan nila jantan lebih cepat pertumbuhannya dari pada ikan nila betina sehingga dapat meningkatkan hasil produksi. Tujuan penelitian ini yaitu untuk mengetahui pengaruh pemberian tepung testis sapi dan dosis terbaik tepung testis sapi dalam pakan yang mengandung  rGH terhadap rasio jenis kelamin, pertumbuhan dan kelulushidupan ikan nila. Bahan uji yang digunakan adalah larva ikan nila dengan stadia larva yang berumur 7 hari setelah lepas kuning telur dan memiliki bobot rata-rata 0,07 gram/ekor. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini yaitu dengan menambahkan tepung testis sapi dengan dosis pada perlakuan A (0%/kg pakan), perlakuan B (3%/kg pakan), perlakuan C (6%/kg pakan) dan, perlakuan D (9%/kg pakan). Pakan pada setiap perlakuan dalam penelitian ini telah ditambahkan rGH dengan dosis 2 mg/kg pakan. Data yang diamati meliputi rasio jenis kelamin, laju pertumbuhan spesifik (SGR), dan tingkat kelulushidupan (SR) ikan nila<em>.</em> Hasil penelitian menunjukan bahwa perlakuan C (6% tepung testis sapi/kg pakan) memberikan hasil terbaik dimana nilai rasio jenis kelamin jantan sebesar 74,44±5,09% dan laju pertumbuhan spesifik sebesar 9,48±0,10% bobot/hari. Kesimpulan yang diperoleh dalam penelitian ini yaitu adanya pengaruh nyata terhadap rasio jenis kelamin dan tingkat pertumbuhan spesifik, namun tidak berpengaruh nyata terhadap tingkat kelulushidupan ikan nila dengan pemberian dosis tepung testis sapi terbaik adalah 6% tepung testis sapi/kg pakan.</p><p><strong>Kata kunci: </strong>Sex reversal; Tepung testis sapi;  Pertumbuhan, rGH; Nila<strong></strong></p><p align="center"><strong>ABSTRACT</strong></p><p><em>Tilapia fish (Oreochromis niloticus)cultivation still continue to be developed until nowdays. Many fisheries technology had been applied, and one of them is sex reversal which is masculinization. Male tilapia fish grows faster than the female one, so this can boost the produce of the fish. This research aims to know the effect of cow testicle flour addition and determine the best dosage on the fish feed which contains rGH towards the male-sex ratio, growth, and survival rate of tilapia fish. The material which used in this research was tilapia fish (O. niloticus) which under the larvae phase 7 days post the separated egg yolk which have average weight of 0,07 grams/each. This research used experimental methods with Completely Randomized Design of 4 treatments and 3 times of repetitions. The treatments in this research was addition of  different dosage of bull testicle which treatment A (0%/kg fish feed),treatment B (3%/kg fish feed), treatment C (6%/kg fish feed) and, treatment D (9%/kg fish feed). The fish feed in every treatment had been added with 2mg rGH/kg fish feed. Data observation  during this research were male-sex ratio, specific growth rate (SGR), and survival rate (SR) of tilapia fish. Results of the research showed that treatment C (6%/kg fish feed) gave the best result as male-sex ratio by 74,44±5,09% and specific growth rate by 9,48±0,10% weight/day. Conclusion obtained from this research was: there was different result  towards the male-sex ratio and specific growth rate, but there was no difference between the treatments towards the survival rate, the best bull testicle flour dosage was 6%/kg fish feed. </em></p><p><strong><em>Keywords:</em></strong><em> Sex reversal, Bull testicle flour, Growth, rGH, Tilapia<strong></strong></em></p>


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