scholarly journals Antimicrobial Activities and Mechanism of Action of Petiveria alliacea Stem Extract

2018 ◽  
Vol 1 (1) ◽  
pp. 45-55
Author(s):  
Yani Mulyani ◽  
Ika Kurnia Sukmawati ◽  
Jajang Jafar Sodik
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Molecular Docking ◽  
Mechanism Of Action ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Petiveria Alliacea

Abstract. This research aimed to determine the antimicrobial activity of ethanol extract of Petiveria alliacea stem (EEPS) against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by perforation and broth micro dilution methods. Study on the mechanism of action of EEPS was conducted by molecular docking and Scanning Electron Microscopy (SEM) techniques. The results showed that EEPS had an inhibitory activity against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans with MIC values of 256, 128, 256, 512 µg/ml, consecutively. These values are included in to the medium category. Through the process of molecular docking, the best interaction was observed between S-benzyl-L-cysteine sulfoxide with penicillin-binding protein receptor of Pseudomonas aeruginosa characterized by free energy change (ΔG) of 4.32 kcal/mol, and the Ki value of 682.16 μM. Four folds of MIC of the EEPS caused changes in the morphology of Pseudomonas aeruginosa. EEPS possessed antimicrobial activities against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans.   Keywords: Antimicrobial Activity, Molecular Docking, Petiveria alliacea, SEM.

scholarly journals UJI AKTIVITAS ANTIMIKROBA EKSTRAK ETANOL ORGANISME LAUT SPONS Ianthella basta TERHADAP BEBERAPA MIKROBA PATOGEN

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 100
Author(s):  
Syifa Sari Katili ◽  
Defny S Wewengkang ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Marine Organism ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Paper Disc

ABSTRACTSponges are multicellular metazoa animals belonging to the Porifera phylum, which has a different structure from other metazoans. The purpose of this study was to determine whether ethanol extracts from the marine organism sponge Ianthella basta have antimicrobial activity against several pathogenic microbes Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. The extraction method used is maceration with 96% ethanol solvent. The method used is the Diffusion Method (Disc Diffusion Kirby and Bauer). The antimicrobial activity test uses a 6 mm paper disc with 50 µL absorption per disc. The results of crude ethanol extract of Ianthella basta sponge from all test microbes, namely Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, were seen to provide the greatest inhibitory activity against Staphylococcus aureus bacteria with an average of inhibitory zone of 7.00 mm categorize as intermediate The results obtained showed that the crude extract of the sponge Ianthella basta has antimicrobial activity because it can inhibit the growth of Staphylococcus aureus, Escherichia coli and Candida albicans microbes even though the inhibition zone is categorized as intermediate. Keywords: Ianthella basta, antimicrobial, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans. ABSTRAKSpons adalah hewan metazoa multiseluler tergolong ke dalam filum Porifera, yang memiliki perbedaan struktur dengan metazoan lainnya. Tujuan penelitian ini ialah untuk mengetahui apakah ekstrak etanol dari organisme laut spons Ianthella basta memiliki aktivitas antimikroba terhadap beberapa mikroba patogen Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa dan Candida albicans. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol 96%. Metode yang digunakan yaitu Metode Difusi (Disc Diffusion Kirby and Bauer).  Pengujian aktivitas antimikroba ini menggunakan kertas cakram (paper disc) berukuran 6 mm dengan daya serap 50 µL tiap cakram. Hasil ekstrak kasar etanol Spons Ianthella basta dari semua mikroba uji yaitu Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa dan Candida albicans, terlihat yang memberikan daya hambat paling besar terdapat pada bakteri Staphylococcus aureus dengan jumlah rata – rata zona hambat yaitu 7,00 mm dengan kategori sedang. Hasil penelitian yang diperoleh menunjukkan bahwa ekstrak kasar dari Spons Ianthella basta memiliki aktivitas antimikroba karena mampu menghambat pertumbuhan mikroba uji Staphylococcus aureus, Escherichia coli dan Candida albicans walaupun dengan zona hambat yang  dikategorikan sedang. Kata Kunci : Ianthella basta, antimikroba, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans.

scholarly journals Synthesis and Antimicrobial Activities of some Quaternary Morpholinium Chlorides

2010 ◽  
Vol 59 (1) ◽  
pp. 49-53 ◽  
Author(s):  
BOGUMIŁ BRYCKI ◽  
ZOFIA DEGA-SZAFRAN ◽  
ILONA MIRSKA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Nitrogen Atom ◽  
Alkyl Group ◽  
Antimicrobial Activities ◽  
Proteus Vulgaris

The synthesis and antimicrobial activity of 31 morpholinium chlorides, divided into five series depending on the substituents attached to the nitrogen atom, N-carboxyalkyl-morpholinium chlorides (1a-e), N-carbalkoxymethyl-N-methyl-morpholinium chlorides (2a-f), N-carbethoxymethyl-N-alkyl-morpholinium chlorides (3a-g), N-carbalkoxymethyl-N-dodecyl-morpholinium chlorides (4a-f) and N-carboxymethyl-N-alkyl-morpholinium chlorides (5a-g) is reported. The compounds investigated were tested for antimicrobial activity against Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Candida albicans and Trichophyton menthagrophytes. The most active are compounds with a long N-alkyl group and with the substituent CH2COOC(n)H(2n-1) (n = 8-16).

scholarly journals Antimicrobial Activities of Novel Bis-Piperidinium Compounds

2012 ◽  
Vol 61 (3) ◽  
pp. 223-225
Author(s):  
MUHAMMAD JAWWAD SAIF ◽  
MOHAMMED ZUBER ◽  
JAMIL ANWAR ◽  
MUNAWAR ALI MUNAWAR
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Alkyl Chains ◽  
Dodecyltrimethylammonium Chloride

The antimicrobial activity of two new series of bis-piperidinium compounds with alkyl chains of different lengths against bacterial (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis) and fungal strains (Aspergillus flavus, Aspergillus niger, Rhodolorula rubera, Lipomyces lopofera and Candida albicans), are described. Antimicrobial activities of the synthesized compounds were compared to that of dodecyltrimethylammonium chloride. Bis-piperidinium salts possessing 12-16 carbon side chains showed better antimicrobial properties as compared to the standard dodecyltrimethylammonium chloride.

scholarly journals UJI DAYA HAMBAT EKSTRAK DAN FRAKSI SPONS Aaptos Aaptos TERHADAP PERTUMBUHAN Escherichia coli, Staphylococcus aureus, dan Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 168
Author(s):  
Sitti N Tunggali ◽  
Herny E. I. Simbala ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Microbial Growth ◽  
Ethanol Extract ◽  
Chloroform Fraction ◽  
Marine Biota ◽  
Disc Diffusion ◽  
Average Value

ABSTRACT Sponge Aaptos aaptos is a marine biota that has great potential, which can be applied, in the pharmaceutical field because of the presence of large compounds in inhibiting microbial growth. This study aims to determine the inhibitory activity of extracts and fractions of sponge Aaptos aaptos on microbial growth of Escherichia coli, Staphylococcus aureus, and Candida albicans. The samples were extracted by maceration with 96 % ethanol and fractioned with n-hexane, choloroform and methanol. Testing is done using the Disc Diffusion Agar method. Crude ethanol extract and fraction of sponge Aaptos aaptos showed the greatest antimicrobial activity against Staphylococcus aureus and categorized as strong, with an average value of 20.32 mm for ethanol extract with strong categories, chloroform fraction 13,28 mm with medium category and methanol fractions 18,48 mm strong category. Keyword: Aaptos aaptos, antimicrobial activity, Escherichia coli, Staphylococcus aureus, Candida albicans.  ABSTRAK Spons Aaptos aaptos merupakan biota laut yang memiliki potensi sebagai antimikroba yang dapat diterapkan di bidang farmasi dengan kandungan senyawa yang besar dalam menghambat pertumbuhan mikroba. Penelitian ini bertujuan untuk mengetahui aktivitas daya hambat dari ekstrak dan fraksi spons Aaptos aaptos terhadap pertumbuhan mikroba Escherichia coli, Staphylococcus aureus, dan Candida albicans. Sampel diekstraksi secara maserasi dengan etanol dan difraksinasi dengan pelarut n–heksan, kloroform dan metanol. Pengujian dilakukan dengan menggunakan metode Disc Diffusion Agar. Ekstrak kasar etanol dan fraksi dari Spons Aaptos aaptos menunjukkan aktivitas antimikroba paling besar terhadap Staphylococcus aureus dan dikategorikan kuat, dengan nilai rata – rata 20,32 mm untuk ekstrak etanol dengan kategori kuat, fraksi kloroform 13,28 mm, kategori sedang dan fraksi metanol 18,48 mm kategori kuat.Kata Kunci : Aaptos aaptos, aktivitas antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans

scholarly journals Antimicrobial activity of soft and purified propolis extracts

Medicina ◽  
2008 ◽  
Vol 44 (12) ◽  
pp. 977 ◽  
Author(s):  
Alvydas Pavilonis ◽  
Algirdas Baranauskas ◽  
Ligita Puidokaitė ◽  
Žaneta Maželienė ◽  
Arūnas Savickas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Phenolic Compounds ◽  
Klebsiella Pneumoniae ◽  
Gram Negative ◽  
Propolis Extract

Objective. To evaluate the antimicrobial activity of soft and purified propolis extracts. Study object and methods. Antimicrobial activity of soft and purified propolis extracts was determined with reference cultures of Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 33499, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 12459, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 8035, and fungus Candida albicans ATCC 60193. Microbiological tests were performed under aseptic conditions. Minimum inhibitory concentration (MIC) – the highest dilution of preparation (the lowest concentration of preparation) that suppresses growth of reference microorganisms – was determined. Results. Concentration of phenolic compounds in soft propolis extract that possesses antimicrobial activity against gram-positive (Staphylococcus aureus, Enterococcus faecalis) and gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis) is 0.587±0.054 mg and 0.587±0.054–0.394±0.022 mg (P>0.05) and in purified propolis extract – 0.427±0.044 mg and 0.256±0.02 mg (P>0.05). Klebsiella pneumoniae is most resistant to soft propolis extract when the concentration of phenolic compounds is 1.119± 0.152 mg and to purified propolis extract when the concentration of phenolic compounds is 1.013±0.189 mg (P>0.05). Spore-forming Bacillus subtilis bacteria are more sensitive to soft and purified propolis extracts when the concentration of phenolic compounds is 0.134±0.002 mg and 0.075±0.025 mg, respectively, and Bacillus cereus – when the concentration is 0.394±0.022 mg and 0.256±0.02 mg (P>0.05). Sensitivity of fungus Candida albicans to soft and purified propolis extracts is the same as Bacillus subtilis. Encapsulated bacterium Klebsiella pneumoniae is most resistant to antimicrobial action of soft and purified propolis extracts as compared with gram-positive Staphylococcus aureus and Enterococcus faecalis bacteria (P<0.05), gram-negative Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis (P<0.05), sporeforming Bacillus subtilis and Bacillus cereus bacteria (P<0.05), and fungus Candida albicans (P<0.05). There is no statistically significant difference between antimicrobial effect of soft propolis extract and purified propolis extract on gram-positive bacteria, gram-negative bacteria, spore-forming bacteria, encapsulated bacteria, and Candida fungus. Conclusions. Soft and purified propolis extracts possess antimicrobial activity. They could be recommended as natural preservatives in the manufacture of pharmaceutical products.

scholarly journals Novel synthetic antimicrobial peptides effective against methicillin-resistant Staphylococcus aureus

1994 ◽  
Vol 302 (2) ◽  
pp. 535-538 ◽  
Author(s):  
J Alvarez-Bravo ◽  
S Kurata ◽  
S Natori
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Liquid Medium ◽  
Culture Medium ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antimicrobial Activities ◽  
Methicillin Resistant ◽  
Synthetic Antimicrobial Peptides

Previously, we identified a core undecapeptide of sapecin B having antimicrobial activity. Based on the structure of this peptide, we systematically synthesized peptides consisting of terminal basic motifs and internal oligo-leucine sequences and examined their antimicrobial activities. Of these peptides, RLKLLLLLRLK-NH2 and KLKLLLLLKLK-NH2 were found to have potent microbicidal activity against Staphylococcus aureus, Escherichia coli, methicillin-resistant S. aureus and Candida albicans in liquid medium. We also synthesized the D-enantiomer of KLKLLLLLKLK-NH2. This enantiomer was resistant to tryptic digestion and persisted longer in the culture medium, showing greater antimicrobial activity than the original peptide.

scholarly journals Characterization and Antimicrobial Activity of Erbium(III) Complexes of C-3 Substituted 2-hydroxy-1,4-naphthalenedione-1-oxime Derivatives

2001 ◽  
Vol 8 (3) ◽  
pp. 159-164 ◽  
Author(s):  
S. B. Jagtap ◽  
N. N. Patil ◽  
B. P. Kapadnis ◽  
B. A. Kulkarni
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Magnetic Susceptibility ◽  
Candida Albicans ◽  
Xanthomonas Campestris ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Oxime Derivatives

Erbium(III) complexes of 2-hydroxy-l,4-naphthalenedione-1-oxime and its C-3 substituted derivatives are synthesized and characterized by elemental analysis, thermogravimetric analysis, infrared spectroscopy, magnetic susceptibility measurements 2-hydroxy-1,4-naphthalenedione-1-oxime derivatives are analysed using H1 and C13 NMR spectroscopy. The molecular composition of the synthesized complexes is found to be [ML3(H2O)2]. The antimicrobial activity of these complexes is determined by well diffusion method against the target microorganisms- Staphylococcus aureus, Xanthomonas campestris, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. The antimicrobial activities of 2- hydroxy-1,4-naphthalenedione-1-oximes and their complexes are compared. It is observed that 2-hydroxy-1,4-naphthalenedione-l-oximes exhibit higher antifungal activity as compared to antibacterial activity. These activities are reduced upon complexation of these oximes with Erbium.

scholarly journals Antimicrobial activity of bioactive component from flower of linum capitatum kit

2004 ◽  
Vol 3 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Slavica Ilic ◽  
Sandra Konstantinovic ◽  
Zoran Todorovic
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Bioactive Components ◽  
Bioactive Component ◽  
Bacillus Subtilus ◽  
Simplex Virus

Different extracts containing bioactive components and etheric oil of the flowers of Linum capitation kit. (Linacea) of Serbian origin were tested for an Antimicrobial activity against four bacteria (Staphylococcus aureus Escherichia coli, Bacillus subtilus, Pseudomonas aeruginosa), one mold (Aspergillus niger) and one yeast (Candida albicans). The isolated Flavonoids were also tested against Staphylococcus aureus, Escherichia coli Bacillus anhtracis, Pseudomonas aeruginosa, Aspergillus niger, Candida albicans and Herpes simplex virus type.

scholarly journals Antibiogram and Phytochemical Analysis of Cinnamon, Clove, and Sichuan Pepper Extracts

2021 ◽  
Vol 9 (1) ◽  
pp. 1-7
Author(s):  
Bibek Adhikari ◽  
Pradeep Kumar Shah ◽  
Roman Karki
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Proteus Mirabilis ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Soxhlet Apparatus ◽  
Medicinal Plant Extracts ◽  
Wide Range

A wide range of medicinal plant extracts has phytochemicals that possess antimicrobial properties and these plants are used to treat several infections. The study aimed to assess the antimicrobial activities of some spices extracts and to evaluate the phytochemicals present in them. The extracts of spices were prepared using Soxhlet apparatus refluxing with methanol and ethanol. The well diffusion technique was implemented for the evaluation of antimicrobial activities of the extracts and the zone of inhibitions was recorded in millimeters. The antimicrobial test was done against five bacterial isolates: Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enterica serotype Typhi, and Staphylococcus aureus and a fungal isolate: Candida albicans. The extracts were concentrated by Rotary Vacuum Evaporator and a stock solution of 200 mg/mL was prepared by dissolving in 10 % DMSO. Concentrations of 40, 60, 80 and 100 mg/mL extracts were used for antimicrobial activity. The result of this study showed that clove extracts had the highest antimicrobial property against all the test microorganisms. Methanolic extract of clove had the highest inhibitory effect against Proteus mirabilis (24.21±0.15 mm), Pseudomonas aeruginosa (19.78±0.23 mm), and Candida albicans (20.07±0.08 mm) whereas ethanolic extract was effective against Escherichia coli (20.44±0.16 mm), Salmonella Typhi (21.66±0.31 mm) and Candida albicans (21.11±0.09 mm). Cinnamon and pepper extracts, leaving some exceptions, also had antimicrobial properties. The presence of phytochemicals: polyphenols, flavonoids, and tannins are the major components responsible for antimicrobial activity. Thereby, this study successfully demonstrated the possibilities of using spices extracts in the treatment of microbial infections.

Sign in / Sign up

Export Citation Format

Share Document