scholarly journals Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

2018 ◽  
Vol 1 (2) ◽  
pp. 41-47
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Mardiana
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories.       Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

scholarly journals Aktivitas Antioksidan Akar Bawang Merah Lokal Palu (Allium cepa Var Aggergatum L.) dengan Berbagai Kepolaran Pelarut

KOVALEN ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 198-205
Author(s):  
Nur Faidah ◽  
Nurhaeni ◽  
Ahmad Ridhay ◽  
Jusman ◽  
Abd. Rahman Razak ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Allium Cepa ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Root Extract ◽  
Polar Solvents ◽  
Ic50 Value ◽  
Dpph Method ◽  
Keywords Antioxidants

Research on the antioxidant activity of the local onion roots of Palu (Allium cepa Var Aggregatum L.) with various polarity solvents has been carried out. This study aims to determine the potential of Palu local shallot root extract as an antioxidant and determine the IC50 value of the Palu local shallot root antioxidant activity. The method applied in this research is maceration using n-hexane, ethyl acetate, and ethanol. The extracts from each solvent were tested for their antioxidant activity by the DPPH method. The results of the study showed that the IC50 value in n-hexane extract, ethyl acetate extract, and ethanol extract were 579.98 ppm, 518.28 ppm 315.83 ppm and as a comparison used ascorbic acid obtained IC50 of 53.69 ppm. Based on the results obtained, the best antioxidant activity is in polar solvents (ethanol extract) compared to other solvents. Keywords: Antioxidants, roots, Palu local shallots, Allium cepa Var Aggregatum L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN DARUJU (Acanthus ilicifolius L.) DENGAN METODE PEREDAMAN RADIKAL BEBAS 1,1-DIPHENYIL-2-PICRYLHIDRAZIL (DPPH)

2018 ◽  
Vol 5 (2) ◽  
pp. 299-308
Author(s):  
Selpida Handayani ◽  
Ahmad Najib ◽  
Nurul Purnama Wati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Extraction Method ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Acanthus Ilicifolius ◽  
Ic50 Value

Sea holly leaves (Acanthus ilicifolius L.) belongs to Acanthaceae family, contain flavonoid compounds, alkaloids, an phenols. This research aimed to determine the antioxidant activity of sea holly leaf extract by free radical damping method 1,1-Diphenyl-2-Picrylhydrazil (DPPH). The extraction method multilevel maseration using n-hexane extract, ethyl acetate extract, and ethanol extract is 1,55%, 0,65% and 4,97% respectively. On each extract, the antioxidant activity was assayed by DPPH free radical inhibition method by measuring is absorbance at the maximum wavelength of 515nm using UV-VIS spectrophometer with quercetin compound as comparator. The result of antioxidant assay showed that IC50 value, ethanol extract is 34,659 μg/mL (strong antioxidant), ethyl acetate extract is 162,512 μg/mL (weak antioxidant), n-hexane extract is361,730 μg/mL (not active as antioxidant).

scholarly journals Antioxidant Activity Test and Determination Of Total Flavonoids Levels of Kedondong Laut (Nothopanax fructicosum (L.) Miq) Leaf Extract

2020 ◽  
Vol 11 (2) ◽  
pp. 2493-2497
Author(s):  
Virsa Handayani ◽  
Andi Amaliah Dahlia ◽  
Nurul Hasanah ◽  
Aulia Nur Siti Nur Aisya Amin
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Flavonoid Compound ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
The Family

Kedondong laut (Nothopanax fruticosum (L.) Miq.) is a plant from the family Araliaceae. Leaves of kedondong laut useful for diuretik, analgesics, as an antidiarrheal and  arthritis. The characteristics of sea kedondong are shrubs, yellowish green, the size of small flowers are green and the fruit is greenish purple and the height of the tree reaches up to 3 meters. Extracted by stratified extraction using solvents n-Hexane, ethyl acetate, and 96% ethanol with a percent rendender for n-Hexane solvent 1.504% ethyl acetate 2.784%, ethanol 3.698%. The results showed that each extract of kedondong laut leaves (Nothopanax fructicosum (L.) Miq has antioxidant activity with IC50 value of n-hexane extract of 33.839µg/mL, ethyl acetate extract of 12.604µg/mL and ethanol extract of 2.222 µg/mL ethanol extract of kedondong laut leaves (Nothopanax fructicosum (L.) Miq) has higher antioxidant power than n-hexane extract and ethyl acetate, the total flavonoid compound content is 0.09902 gQE/g extract or 9.902%, ethyl acetate extract is equal to 0.13253 gQE /g extract or 13.253%, and 96% ethanol extract at 0.09345 gQE / g extract or 9.345%, ethyl acetate extract has a greater flavonoid content than n-hexane extract and 96% ethanol.

scholarly journals Ethanol Extract, Ethyl Acetate Extract, Ethyl Acetate Fraction, and n-Heksan Fraction Mangosteen Peels (Garcinia mangostana L.) As Source of Bioactive Substance Free-Radical Scavengers

2016 ◽  
Vol 1 (2) ◽  
pp. 71
Author(s):  
Liza Pratiwi ◽  
Achmad Fudholi ◽  
Ronny Martien ◽  
Suwidjiyo Pramono
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Garcinia Mangostana ◽  
Ic50 Value ◽  
Dpph Method ◽  
Peel Extract

<span>Mangosteen peels (Garcinia mangostana L.) is well known as an excellent source of antioxidative compounds. The name of mangosteen is xanthone. Antioxidant of mangosteen  peels can be extracted by ethanol, etyl acetate and can be fractinated by etyl acetate and n-hexane. The aim of this research was to compare the antioxidant activity of the peel extract by ethanol and etyl acetate and fractinated by etyl acetate and n-hexane. Extract and fraction exhibited higher scavenging activity of DPPH. </span><span lang="EN">The purpose of this study was to compare antioxidant activity of ethanol extract, ethyl acetate extract, fraction of ethyl acetate and n-hexane fraction.</span><span> The</span><span lang="EN"> antioxidant activity </span><span>test </span><span lang="EN">using DPPH method with UV-Vis spectrophotometer</span><span>. Ethanol extract shown IC50 value 5,03 µg/mL, ethyl acetate extract shown IC50 value 41,56 µg/mL. Ethyl acetate fraction shown IC50 value 2,78 µg/mL, and n-hexane fraction with IC50 22,33 µg/mL. It means peel extract and fraction by mangosteen peels has very strong antioxidant activity and ethyl acetate fraction that its antioxidant activity higher that the other solvent.</span>

scholarly journals The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells

2021 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Dwi Mahfud Maulana
Keyword(s):  
Hela Cell ◽  
Cell Viability ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Rhizophora Apiculata ◽  
Ic50 Value ◽  
Ethyl Acetate Extracts

Disease cancer caused by abnormal growth of tissue where there has been an error, fast and out of control. Judging from the fact of gender, more than 270,000 women die every year caused by cervical cancer. To inhibit the growth of cancer cells, a compound is needed that causes the cell cycle to stop so that the ability of cell proliferation decreases. Alkaloid compounds can inhibit proliferation through oxidative inhibition processes that can cause cancer. Mangrove plants have potential as anticancer, antimicrobial, and antioxidant. The content of chemical compounds found in mangroves are flavonoids, steroids, alkaloids, phenolites, saponins and tannins. These compounds show high antioxidant activity and are shown to have a real relationship with the properties of the material's bioactivity against cancer cells. One of the mangrove species is Rhizophora apiculata. The purpose of this study was to determine the IC50 value produced by Rhizophora apiculata mangrove leaf extract on HeLa cell viability and to see the effect of Rhizophora apiculata mangrove leaf extract dosage on HeLa cell viability. The method used in this research is the experimental method. The research parameters included yield, proximate test, phytochemical test, toxicity test, total phenol test, cytotoxicity test and LC-MS test. The experimental design used was a simple and complex completely randomized design (CRD) with the Tukey test.The results of this study showed that the highest yield was in the ethanol extract of 5.91%, while the n-hexane and ethyl acetate extracts respectively had yields of 1.18% and 1.31%. The results of the proximate test on the water content of leaves and powder were 64.53% and 13.86%, respectively, the results of the ash content in the leaves and powder of Rhizophora apiculata were 3.94% and 8.41%, respectively. while the water content in the extract obtained the highest yield in the ethanol extract of 21.42%, while the n-hexane extract and ethyl acetate extract were 11.08% and 15.42%, respectively. For phytochemical results, it was found that n-hexane extract only contained alkaloids, flavonoids and steroids. Ethyl acetate extract contains steroid compounds. Meanwhile, the ethanol extract contains the most bioactive compounds, namely saponins, flavonoids, tannins and triterpenoids. The toxicity test using the Brine Shrimp Lethality Test (BSLT) method resulted in the lowest IC50 of ethanol extract at 49.45 ppm while the n-hexane and ethyl acetate extracts were 251.63 ppm and 920.45 ppm respectively. In the total phenol test, the n-hexane extract was 66.79 mg GAE / 100 gr, 222.97 mg GAE / 100 gr ethyl acetate extract and 929.04 mg GAE / 100 gr ethanol extract. HeLa cell cytotoxicity testing using the MTT method (3- (4,5-dimethiltiazol-2-yl) -2,5-dipheniltetra zolium bromide) assay resulted in the highest cell viability value at a dose of 125 ppm of 46.97%. As for the doses of 250 ppm, 500 ppm 1000 ppm, and 2000 ppm resulted in a percentage of viability of 42.95% 37.70% 35.82% and 32.12%, respectively. The IC50 value of Rhizophora apiculata leaf extract was 64.42 ppm. This value indicates that the Rhizophora apiculata extract is toxic to HeLa cells.

scholarly journals Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp

2020 ◽  
Vol 151 ◽  
pp. 01029
Author(s):  
Nuzul Asmilia ◽  
Mahdi Abrar ◽  
Yudha Fahrimal ◽  
Amalia Sutriana ◽  
Yobeswi Husna
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Hexane Extract ◽  
Clear Zone ◽  
Inhibitory Property

Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.

scholarly journals SKRINING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN, DAN TOKSISITAS DARI EKSTRAK DAUN KERSEN (Muntingia calabura L.) DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT)

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 315
Author(s):  
Selin Widjaya ◽  
Widdhi Bodhi ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Brine Shrimp Lethality ◽  
Phytochemical Content ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Dpph Method ◽  
Muntingia Calabura

ABSTRACTKersen (Muntingia calabura L.) is a plant that has begun to be eliminated and was rarely used because it is often considered to have no economic value and lack of knowledge about its utilization, whereas kersen plants contain flavonoids, saponins, and tannins which were have high benefit for health. The content of metabolites is affected by soil nutrient elements and difference place of growth. This study aims to determine the potential of kersen leaves grown in North Minahasa based on phytochemical content, ability of antioxidant activity, and toxicity. Kersen leaves were extracted using sequential maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Phytochemical Screening using several reagents which tailored to the type of phytochemical test. 1.1-diphenyl-2-picrylhydrazyl (DPPH) method is used to evaluate antioxidant activity, and Brine Shrimp Lethality Test (BSLT) method is used to evaluate toxicity. The result of this study indicate that the n-hexane extract contains phenols, flavonoids, and tannins, with IC50 value 12.54 μg/mL, and LC50 value 881 μg/mL. Ethyl acetate extract contains phenols, flavonoids, tannins, and saponins, with IC50 value 61.3 μg/mL, and LC50 value 1758 μg/mL. Ethanol extract has phenol, flavonoid, tannin, saponin, and terpenoid content, with IC50 value 9.01 μg/mL, and LC50 value 106 μg/mL. Keywords : Kersen leaves, Antioxidant, Toxicity, IC50, LC50 ABSTRAKKersen (Muntingia calabura L.) merupakan tanaman yang sudah mulai tersingkirkan dan jarang dimanfaatkan karena sering dianggap tidak punya nilai ekonomis dan kurangnya pengetahuan tentang pemanfaatannya, padahal tanaman kersen memiliki kandungan flavonoid, saponin, dan tanin yang bermanfaat tinggi untuk kesehatan. Kandungan senyawa metabolit dipengaruhi oleh unsur hara tanah dan perbedaan tempat tumbuh. Penelitian ini bertujuan untuk mengetahui potensi dari daun kersen yang tumbuh di Minahasa Utara berdasarkan kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitasnya. Ekstrak daun kersen diekstraksi dengan metode maserasi sekuensial menggunakan pelarut n-heksan, etil asetat, dan etanol.  Skrining fitokimia menggunakan beberapa reagen yang disesuaikan dengan jenis uji fitokimia. Metode 1.1-diphenyl-2-picrylhydrazyl (DPPH) digunakan untuk mengevaluasi aktivitas antioksidan, dan metode Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi toksisitas. Hasil penelitian ini menunjukkan bahwa ekstrak n-heksan memiliki kandungan fenol, flavonoid, dan tanin, nilai IC50 12,54 μg/mL, dan nilai LC50 881 μg/mL. Ekstrak etil asetat memiliki kandungan fenol, flavonoid, tanin, dan saponin, nilai IC50 61,3 μg/mL, dan nilai LC50 1758 μg/mL. Ekstrak etanol memiliki kandungan fenol, flavonoid, tanin, saponin, dan terpenoid, nilai IC50 9,01 μg/mL, dan nilai LC50 106 μg/mL. Kata kunci : Daun Kersen, Antioksidan, Toksisitas, IC50, LC50

scholarly journals Penyarian Konstituen Organik Daging Buah Gaharu (Aquilaria malaccensis Lamk.) dengan Metode Maserasi Berbantu Microwave dan Uji Aktivitas Sebagai Antioksidan

KOVALEN ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 42-50
Author(s):  
Imran ◽  
Nurlian ◽  
L.A. Kadir ◽  
La Agusu ◽  
Ruslan
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Hexane Extract ◽  
Fruit Pulp ◽  
Microwave Assisted ◽  
Organic Constituents ◽  
Aquilaria Malaccensis ◽  
Ic50 Value

This study was conducted to determine the organic constituents and to test the antioxidant activity and toxicity of gaharu fruit pulp extract (Aquilaria malaccensis Lamk.). Gaharu fruit pulp extract was obtained through Microwave Assisted Extraction (MAE) method and then extracted with a solvent with different polarity, namely ethyl acetate and n-hexane. The obtained yield of ethyl acetate extract of the gaharu fruit pulp was 40.827% w/v, whereas 0% w/v in n-hexane extract, therefore, no further test for n-hexane extract, so that the n-hexane extract was not tested for the next stage. The results of the organic constituents of the ethyl acetate extract of gaharu fruit pulp showed flavonoids, saponins, glycosides, phenols and tannins. Antioxidant activity testing was carried out using the DPPH method with vitamin C as a positive control. The results of the antioxidant activity test showed that IC50 of ethyl acetate extract of gaharu fruit pulp and vitamin C were 143.789ppm and 13.797ppm, respectively. Extracts from microwave-assisted maceration that were partitioned with ethyl acetate solvent were categorized into moderate antioxidants because the IC50 value was between 101-150ppm, while vitamin C was categorized as a strong antioxidant because the IC50 value was between 0-100ppm. Toxicity testing was also carried out on ethyl acetate extract of gaharu fruit pulp using the BSLT method. The results of the toxicity test of the ethyl acetate extract of gaharu fruit pulp showed activity with an LC50 value of 11.282ppm. Based on this research, the ethyl acetate extract of gaharu fruit pulp is considered to have an anticancer potential.

AKTIVITAS ANTIOKSIDAN EKSTRAK UMBI SUWEG (Amorphophallus paeoniifolius) DARI BERBAGAI TINGKAT POLARITAS PELARUT

KOVALEN ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Deddy Firman ◽  
Nurhaeni Nurhaeni ◽  
Ahmad Ridhay
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Polar Solvent ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Tuber Type

ABSTRACT Has done research about antioxidant activity of suweg tuber (Amorphophallus campamulatus) based on the level of polar solvent. This research begins with in phased maceration extraction using n-hexane, ethyl acetate dan ethanol. Next step was antioxidant activity test with tyocyanate to n-hexane extract and DPPH methods to ethanol extract, ethyl acetate and vitamin C. The result showed antioxidant activity in n-hexane extract i.e. 14,96 %, end 77,13 % BHT while in ethyl acetate, ethanol and vitamin C reached IC50 value are 458,102 ppm, 223,268 ppm dan 26,76 ppm. Keywords: Suweg tuber, type of solvent, maceration, antioxidant

AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA

EKOLOGIA ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 34-38
Author(s):  
Siti Warnasih ◽  
Diana Widiastuti ◽  
Uswatun Hasanah ◽  
Laksmi Ambarsari ◽  
Purwantiningsih Sugita
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Soxhlet Extraction ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Palm Fruit ◽  
Ic50 Value ◽  
Dpph Method ◽  
Fruit Processing

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

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