Antioxidant activity of winter wheat when treated with the biopreparation Nigor++

The influence of stress factors on the plant can lead to deterioration of plant functioning and loss of yield. The use of biological preparations based on plant cell elicitors in the technology of grain cultivation makes it possible to regulate the antioxidant activity of plants under abiotic stress. Treatment with the biopreparation Nigor++ in the tillering phase leads to noticeable changes in the balance of antioxidant substances in winter wheat plants Moskovskaya 39. Repeated treatment of Nigor++ after 16 days in the earing phase reduces AOA by 21.8, and by the flowering phase leads to a drop in this indicator by 51% relative to the tillering phase and by 59% relative to control plants. The use of Nigor++ increases the immune status of plants, reduces the concentration of antioxidant substances or reduces their activity due to the formation of stable complexes with magnesium ions included in BP, as well as binding them to highly active free radicals that occur during autoxidation, converting them to low-activity. Keywords: ANTIOXIDANTS, BIOLOGICAL PRODUCTS, WINTER WHEAT, ANTIOXIDANT ACTIVITY, REACTIVE OXYGEN SPECIES, ELICITORS, BIOFLAVONOIDS

UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL SPONS (Leucetta chagosensis) YANG DIKOLEKSI DARI KEPULAUAN MANTEHAGE

ABSTRACTSponges are not only rich in secondary metabolites, but also have the ability to synthesize various compounds, such as alkaloids, peptides, and terpenoids. These compounds are reported to have activity as antitumor, antifungal, antibacterial, and also as an antioxidant. This study aims to determine the antioxidant activity of the marine animal sponge Leucetta chagosensis. This research is a laboratory experiment with testing of the ethanol extract of sponge Leucetta chagosensis with the DPPH [1,1-diphenyl-2-picrylhydrazyl method] to analyze antioxidant activity using a UV-Vis spectrophotometer with a wavelength of 517 nm. The results of this study indicate that the ethanol extract of the sponge Leucetta chagosensis from Mantehage Island has antioxidant activity in each concentration of the test. The greatest antioxidant levels were found in the sponge Leucetta chagosensis with a concentration of 0.6 mg / L, reaching a percentage of 57.23%..Keywords: Antioxidants, DPPH, Leucetta chagosensis, Mantehage Islands ABSTRAKSponge tidak hanya kaya akan metabolit sekunder, tapi juga memiliki kemampuan untuk menyintesis berbagai macam senyawa, seperti alkaloid, peptide, dan terpenoid. Senyawa-senyawa tersebut dilaporkan memiliki aktifitas sebagai antitumor, antijamur, antibakteri, dan juga sebagai antioksidan. Penelitian ini bertujuan Untuk mengetahui aktivitas antioksidan dari hewan laut sponge Leucetta chagosensis. Penelitian ini merupakan eksperimental laboratorium dengan pengujian terhadap ektrak etanol sponge Leucetta chagosensis dengan metode DPPH [1,1-difenil-2-pikrilhidrazil] untuk menganalisis aktivitas antioksidan dengan menggunakan spektrofotometer UV-Vis dengan panjang gelombang 517 nm. Hasil penelitian ini menunjukkan bahwa ekstrak etanol sponge Leucetta chagosensis dari Pulau Mantehage mempunyai aktivitas antioksidan disetiap konsentrasi pengujian. Kadar antioksidan yang paling besar terdapat pada sponge Leucetta chagosensis dengan konsentrasi 0,6 mg/L dengan mencapai persentase 57,23% . Kata kunci: Antioksidan, DPPH, Leucetta chagosensis, Pulau Mantehage

KARAKTERISTIK KIMIA DAN UJI AKTIVITAS ANTIOKSIDAN RUMPUT LAUT (E. spinosum) YANG DIPEROLEH DARI KECAMATAN WANGI-WANGI SELATAN DAN KECAMATAN KALEDUPA SELATAN

ABSTRACT The aim of this study was to determine the proximate content (water content, carbohydrate levels), phytochemicals (alkaloids, flavonoids, saponins, steroids, terpenoids), and antioxidants. Data analysis in this study used descriptive analysis to provide a general description of the data. The results of this study aim to determine the content of proximate (water content, carbohydrate levels), phytochemicals (alkaloids, flavonoids, saponins, steroids, terpenoids), and antioxidants. Proximate test on E. spinosum seaweed has a difference, namely water content with sample code P1 = 41.83% and P2 = 49.19. High water content is in the sample code P2, while the carbohydrate content with sample code P1 = 22.20 and P2 = 17.64. High carbohydrate levels are found in the sample code P1. Phytochemical tests on E. spinosum seaweed with codes P1 and P2 contained flavonoids, saponins, and triterpenoids but there were no alkaloids and steroids. The antioxidant activity test on E. spinosum seaweed in both samples was very weak with sample code P1 having IC50 value = 223.90 while IC50 value at P2 = 222.34.Keywords: Antioxidants, Eucheuma spinosum, Phytochemicals and ProximateABSTRAKPeneletian ini bertujuan untuk mengetahui kandungan proksimat (kadar air, kadar karbohidrat), fitokimia (alkaloid, flavonoid, saponin, steroid, terpenoid) dan antioksidan. Analisis data penelitian ini menggunakan analisis deskriptif untuk memberikan gambaran umum tentang data. Hasil uji proksimat pada rumput laut E. spinosum memiliki perbedaan yaitu kadar air dengan kode sampel P1 = 41.83% dan P2 =49.19. Kadar air tinggi terdapat pada kode sampel P2, sedangkan kadar karbohidrat dengan kode sampel P1 = 22.20 dan P2 = 17.64. Kadar karbohidrat yang tinggi terdapat pada kode sampel P1. Uji fitokimia pada rumput laut E. spinosum dengan kode sampe P1 dan P2 terdapat flavonoid, saponin dan terpenoid namun tidak terdapat alkaloid dan steroid. Uji aktivitas antioksidan pada rumput laut E. spinosum pada kedua sampel sangat lemah dengan kode sampel P1 memiliki nilai IC50 = 223,90 sedangkan nilai IC50 pada P2 = 222,34. Kata kunci: Antioksidan, Eucheuma spinosum, Fitokimia dan Proksimat.

KARAKTERISTIK KIMIA DAN UJI AKTIVITAS ANTIOKSIDAN RUMPUT LAUT (Sargassum sp.) YANG DIPEROLEH DARI KECAMATAN WANGI-WANGI DAN KECAMATAN WANGI-WANGI SELATAN KABUPATEN WAKATOBI

ABSTRACT The aim of this study was to determine the proximate content (moisture content, carbohydrate content), phytochemicals (alkaloids, flavonoids, saponins, steroids, terpenoids) and antioxidants. This research used descriptive analysis. Proximate test results on Sargassum sp. P1 = 35.43% and P2 = 34.42% High water content is found in sample code P1, while carbohydrates P1 = 11.85. and P2 = 22.05. Phytochemical test of seaweed Sargassum sp. there are flavonoids, saponins, and terpenoids but there are no alkaloids and steroids. Antioxidant activity of seaweed Sargassum sp. P1 has a value of IC50 = 153.37 while the value of IC50 is at P2 = 162.32. Keywords: Antioxidants, Phytochemicals Sargassum sp, and Proximate.ABSTRAKTujuan penelitian ini untuk mengetahui karakterisasi kimia aktivitas antioksidan rumput laut Sargassum sp. yang diperoleh dari Kecamatan Wangi-Wangi dan Kecamatan Wangi-Wangi selatan Kabupaten Wakatobi. Penelitian ini menggunakan analisis deskriptif. Hasil Uji proksimat pada rumput laut Sargassum sp. yaitu : P1 = 35,43% dan P2 = 34,42% Kadar air yang tinggi terdapat pada kode sampel P1, Sedangkan karbohidrat dengan kode sampel P1= 11,85. dan P2 = 22,05. Uji fitokimia rumput laut Sargassum sp. terdapat flavonoid, saponin dan terpenoid namun tidak terdapat alkaloid dan steroid. Aktivitas antioksidan rumput laut Sargassum sp. P1 memiliki nilai IC50 = 153,37, sedangkan nilai IC50 pada P2 = 162,32.Kata kunci: Antioksidan, Fitokimia Sargassum sp, dan Proksimat.

Aktivitas Antioksidan Akar Bawang Merah Lokal Palu (Allium cepa Var Aggergatum L.) dengan Berbagai Kepolaran Pelarut

Research on the antioxidant activity of the local onion roots of Palu (Allium cepa Var Aggregatum L.) with various polarity solvents has been carried out. This study aims to determine the potential of Palu local shallot root extract as an antioxidant and determine the IC50 value of the Palu local shallot root antioxidant activity. The method applied in this research is maceration using n-hexane, ethyl acetate, and ethanol. The extracts from each solvent were tested for their antioxidant activity by the DPPH method. The results of the study showed that the IC50 value in n-hexane extract, ethyl acetate extract, and ethanol extract were 579.98 ppm, 518.28 ppm 315.83 ppm and as a comparison used ascorbic acid obtained IC50 of 53.69 ppm. Based on the results obtained, the best antioxidant activity is in polar solvents (ethanol extract) compared to other solvents. Keywords: Antioxidants, roots, Palu local shallots, Allium cepa Var Aggregatum L.

Design of Trolox Compounds as Antioxidant and Their Analysis Using Quantitative Structure Activity Relationship

Antioxidant compound can inhibit the oxidation of lipids and other biomolecules. The role of antioxidants is very important in neutralizing and destroying free radicals that can cause the damage to cells in the body. This research was carried out to design trolox derivate compounds as antioxidants using the QSAR method. The semi empirical AM1(Austin Model 1)method was used to generate the QSAR model. The statistical analysis result using multiple linier regression methods revealed thet antioxidant activity was influenced by the descriptors of qC1, qC4, qO7, qC13 and qO18. The QSAR equation model obtained was log IC50 = 0.821 + 7.067 (qC1) + 2.585 (qC4) + 4.812 (qO7) – 5.363 (qC13) – 0.887 (qO18) with the best predicted IC50 value was 4.699 µM. Keywords: Antioxidants, QSAR, semi empirical AM1, trolox

UJI AKTIVITAS ANTIOKSIDAN Stylissa sp. DENGAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)

ABSTRACTThis study aims to analyze the antioxidant activity of Stylissa sp., sponge. The Stylissa sp., sponge sample was obtained from the Lembeh Strait, waters Bitung. This research is an experimental laboratory by testing the ethanol extracts of Stylissa sp., sponge using DPPH method (1,1-diphenil2-pikrihidrazil) to analyze antioxidant activity using a spectrophotometer. The results of this study show the antioxidant levels of the Stylissa sp., sponge in the Lembeh Strait waters have antioxidant activity and the higher the concentration the higher the antioxidant levels produced. The greatest antioxidant level is found in Stylissa sp., sponge with a concentration of 100 mg / L. Keywords : Antioxidants, DPPH, Sponge Stylissa sp ABSTRAKPenelitian ini bertujuan untuk menganalisis aktivitas antioksidan dari Stylissa sp. Sampel Spons Stylissa sp di peroleh dari perairan Selat Lembeh, Bitung. Penelitian ini merupakan eksperimental laboratorium dengan pengujian terhadap ektrak etanol Spons Stylissa sp dengan metode DPPH (1,1-diphenil-2-pikrihidrazil) untuk menganalisis aktivitas antioksidan dengan menggunakan sprektrofotometer. Hasil penelitian ini memperlihatkan kadar antioksidan dari Spons Stylissa sp di perairan Selat Lembeh mempunyai aktivitas antioksidan dan semakin tinggi konsenstrasi semakin tinggi pula kadar antioksidan yang dihasilkan. Kadar antioksidan yang paling besar terdapat pada Spons Stylissa sp dengan konsentrasi 100 mg/L.Kata kunci : Antioksidan, DPPH, Spons Stylissa sp

Formulasi Masker Peel Off Ekstrak Etanol Batang Saluang Belum Sebagai Antioksidan

ABSTRAK Salah satu tumbuhan yang mempunyai manfaat yang besar terutama sebagai antikosidan adalah Saluang Belum.Tumbuhan ini merupakan Tumbuhan Hutan Berkhasiat Obat (THBO) asal Kalimantan Tengah yang secara empiris digunakan oleh masyarakat Dayak terutama pada daerah Kabupaten Katingan sebagai obat awet muda. Hasil uji identifikasi simpisia Batang Saluang Belum menunjukkan hasil positif pada uji senyawa kimia tanin dan saponin. Berdasarkan hal tersebut, maka peneliti bermaksud untuk mengembangkan produk masker peel off dari ekstrak etanol batang saluang belum yang dapat meningkatkan nilai ekonomi dari THBO. Terdapat 4 (empat) formulasi masker peel off ekstrak etanol batang saluang belum yang berbeda konsentrasi, mulai dari 0%; 1%; 5%; dan 15%. Dari keempat formula masker peel off ekstrak etanol batang Saluang belum yang dihasilkan, formula 1 merupakan formulasi terbaik berdasarkan hasil uji fisik yang dilakukan. Kata Kunci : Antioksidan, Masker peel off, Saluang Belum ABSTRACT One of the plants that has great benefits as antioxidant is Saluang Belum. This plant is Medicinal Forest Plants from Central Kalimantan which used by Dayak community, especially in the Katingan Regency area as a youthful medicine. The results of the chemical compound identification test of Batang Saluang Belum have shown positive results on the test of tannin and saponin. Based on this, the research conducted to develop skin mask products from ethanol extracts of Saluang Belum stems which can increase the economic value of medicinal forest plants. There are 4 (four) peel off mask formulations of ethanol extracts of saluang stem which have different concentrations, starting from 0%; 1%; 5%; and 15%. Of the four peel off mask formulas of ethanol extract of Saluang stem that have not been produced, formula 1 is the best formulation based on the results of physical tests conducted. Keywords: Antioxidants, Peel Masks, Saluang Belum

ANALISIS SENSORI, ANTIOKSIDAN DAN KANDUNGAN SERAT MINUMAN FUNGSIONAL RUMPUT LAUT Eucheuma spinosum DENGAN PENAMBAHAN RUMPUT LAUT Sargassum sp.

ABSTRACT Eucheuma and Sargassum sp. has studied for their potential as a raw material for health product such as functional beverage and food supplements. This study aims to the sensory, antioxidant activity and the fiber content of jelly drinks by using two types of seaweed at different concentrations. Sensory assessment method based on descriptive testing of 25 panelists, antioxidants were measured using the DPPH method, and fiber content were measured based on gravimetric methods. This study used a Randomized Complete Design (CRD) with four treatments of Eucheuma spinosum and Sargassum sp. at consecutive concentrations %:0% (E7S0), 6%:1% (E6S1), 5%:2% (E5S2), 4%:3% (E4S3). Based on the results of the test, showed that the different concentrations of seaweed significantly affected the sensory value of appearance, taste, smell, color, and texture. The highest hedonic test was E7S0, specifications of the resulting jelly drink that has a bright appearance and clean, bright purple, slightly felt and smelled of seaweed and has a chewy texture, antioxidant values (IC50 value) and crude fiber content of E7S0 were 1122.9 ppm and 0.33%. Keywords: antioxidants, Eucheuma spinosum, Jelly drinks, Sargassum sp., crude fiberABSTRAKRumput laut jenis Eucheuma dan Sargassum sp. telah banyak diteliti potensinya sebagai bahan baku untuk produk kesehatan seperti minuman fungsional dan suplemen makanan. Penelitian ini bertujuan untuk mengetahui sifat sensori, aktivitas antioksidan dan kandungan serat minuman jelly dengan penggunaan dua jenis rumput laut pada konsentrasi yang berbeda. Metode penilaian sensori berdasarkan pengujian deskriptif 25 orang panelis, antioksidan diukur menggunakan meode DPPH, dan kadar serat diukur berdasarkan metode gravimetri. Penelitian ini menggunakan Rancangan Acak Lengkakap (RAL) dengan empat perlakuan yaitu dengan penggunaan Eucheuma spinosum dan Sargassum sp. pada konsentrasi berturut-turut: 7%:0% (E7S0), 6%:1% (E6S1), 5%:2% (E5S2), 4%:3% (E4S3). Berdasarkan hasil uji, menunjukkan bahwa penggunaan konsentrasi rumput laut yang berbeda berpengaruh nyata terhadap nilai sensori kenampakan, rasa, aroma, warna dan tekstur. Minuman jelly dengan nilai sensori tertinggi yaitu pada sampel E7S0, dengan spesifikasi minuman jelly yang dihasilkan memiliki kenampakan yang cerah, bersih dan menarik, berwarna ungu cerah, agak terasa dan tercium aroma rumput laut, serta memiliki tekstur yang kenyal. Kapasitas antioksidan (nilai IC50) dan dan kandungan serat kasar pada sampel E7S0 masing-masing sebesar 1122,9 ppm sebesar 0,33%.Kata kunci: antioksidan, Eucheuma spinosum, minuman jelly, Sargassum sp., serat kasar

PROBIOTIC ADMINISTRATION IN PERIODONTAL THERAPY – A RANDOMIZED CONTROLLED CLINICAL TRIAL

Background: The mouth, like the rest of the gastrointestinal tract, is heavily colonized by bacteria. The gingival crevice between the teeth and the gums / gingivae offers a unique niche for colonization of bacteria. Gum disease or ‘periodontitis’ is largely attributed to a microbial etiology along with recent observations on the role of the host response. Hence, periodontal therapy has traditionally focused on the reduction of the bacterial threat. This, however, disrupts the microbial ecological balance. Probiotics is a newer treatment approach that focuses on restoring the reduced numbers of beneficial bacteria. Methods: The present study intended to evaluate the effect of probiotic capsule administration in 20 patients each with gingivitis and periodontitis. ‘Cases’ received probiotic capsule and oral prophylaxis while the remaining ten patients received only oral prophylaxis and served as ‘Controls’ in each of the gingivitis / periodontitis groups. Plaque, Bleeding and Gingival Indices, Pocket probing depth along with GCF samples for estimation of Myeloperoxide and Thiol antioxidants were recorded at baseline and one month after treatment. Results: Improvement of clinical parameters as well as increase in GCF thiol and decreased myeloperoxidase concentrations was observed in the groups with probiotics. Discussion: A greater reduction in plaque scores in the cases may be attributed to probiotic induced reduced microbial colonization. Probiotic species effectively reduce the level of inflammation associated molecules, such as prostaglandin E2 and interferon C and MMP activity. It may be plausible that by modulating these factors, probiotics can lead to improvement of the periodontal parameters. Conclusion: Hence, probiotic supplementation along with scaling and root planing may be an effective adjunct for periodontal therapy. Keywords: Antioxidants, capsule, gingival crevicular fluid, gingivitis, periodontitis, probiotics