EFFECT OF Cr (III) IONS CONCENTRATION ON WAY OF LIFE SPECIFICS, TROPHIC AND DIGESTIVE PARAMETERS OF CERTAIN LIFE FORMS OF POND SNAILS (МOLLUSCA, GASTROPODA, LYMNAEIDAE)

Author(s):  
Stadnychenko A.P. ◽  
Uvayeva O.I. ◽  
Ihnatenko О.О.

For the first time, the influence ofCr (III) ionsin maximum concentration limits (MCL) from 0.5 –3 on the way of life, nutrition and digestion of Lymnaea vulnerata (Küster, 1862) and L. monnardi (Hartmann, 1844), –the different life forms of pondsnailsof the hydrographicnetwork of Ukraine,–was explored. Due to the long-term natural selectionamongthese specieswhich took place in extremely different ecological environmental conditions, the adaptive radiation of the shell of the ancient original form of pondsnailwas carried out by rheophilicL. monnardi and stagnophilicL. vulnerata,in opposite directions -divergently.The ampla-shaped shell of L. monnardi (flattened, low, compact, streamlined, with a large mouth) and a large flat sole prevent its driftingby thewater current, while the stagnicola-liketurret-shapeshell of L. vulnerata (elongated, high, narrow, with curls and flat turns, andsmall mouth)reliably protects it from excessive water consumption in the process of pulmonary respiration, thatis important for this ordinary inhabitant of mostly drying out ponds, experiencing conditions of desiccation, which hidesin the cracks of drying out bottom.The autochthonous fodder diet of these pondsnailsis represented by algae and higher aquatic vegetation (different in species composition for each of the species of mollusks studied).Under the maximum concentration limits of 0.5, MCL, 2MCL, 3MCLof Cr(III) ions in the environment, a 5-phase process of poisoningis being developed among the pond snails(0.5MCL-indifference; MCLand 2MCL-stimulation; 3MCL-depressive, sublethal, lethal phases). Its symptoms are clearly illustrated by the values ofthe main trophological parameters in poisoned individuals, depending on the course of the relevant phases of the pathological process.Key words : Lymnaea, life forms, Cr (III)ions, way of life, nutrition, digestion. Серед полютантів, які потрапляють із скидами чи стоками як у природні, так і у штучні водойми, одними з найнебезпечніших для гідробіонтів є іони важких металів. Актуальність дослідження впливу іонів важких металів на різні прояви життєдіяльності гідробіонтів пов’язана як із широкою розповсюдженістю їх наразі у поверхневих водах, так і з явно недостатнім на сьогодні ступенем дослідженості впливу значної частини із них на водних черевоногих молюсків. Саме до числа таких важких металів і належить Cr (III), рівень вмісту якого у гідромережі України. Уперше з’ясовановплив 0,5ГДКр –3ГДКр іонівCr (III) на спосіб життя, живлення і травлення Lymnaea vulnerata(Küster, 1862) i L. monnardi (Hartmann, 1844) –різних життєвих форм ставковиків гідромережі України. Внаслідок тривалого природного добору, котрий відбувався у цих тварин у вкрай відмінних екологічних умовах довкілля, адаптивна радіація черепашки прадавньої вихідної форми ставковиків здійснювалася реофілом L. monnardi і стагнофілом L. vulnerataу протилежно скерованих напрямках –дивергентно. Амплаподібна черепашка L. monnardi (сплющена, низька, компактна, обтічної форми, з великим устям) і велика плоска підошва ноги запобігають знесенням її течією, тоді як стагніколаподібна баштоподібна черепашка L. vulnerata(витягнутої форми, висока, вузька, з плоскими обертамизавитка і невеличким устям) надійно оберігає його від зайвих витрат води у процесі легеневого дихання, що важливо для цього звичайного мешканця здебільшого пересихаючих водойм, переживаючих умови десикації, забившись у щілини розтрісканого від пересиханнядна.Автохтонний кормовий раціон цих ставковиків представлений водоростями і вищою водяною рослинністю (різними за видовим складом щодо кожного з видів досліджених молюсків).За 0,5ГДКр, ГДКр, 2ГДКр, 3ГДКр іонів Cr (III) у середовищі у ставковиків розвивається 5-тифазний процес отруєння (0,5 ГДКр –байдужість; ГДКр і 2ГДКр –стимуляція; 3ГДКр –депресивна, сублетальна, летальна фази). Симптоматика його чітко ілюструється значеннями основних трофологічних показників у отруєних особин, залежних від перебігу у них відповідних фаз патологічного процесу.Ключові слова: Lymnaea, життєві форми, Cr (III), спосіб життя, живлення, травлення

2018 ◽  
Vol 29 (2) ◽  
Author(s):  
N. O. Roshchyna

In this article, the current and former distribution of higher aquatic vegetation has been analyzed for floodplain lakes, arenas lakes and third terraces lakes in the valleys of large and medium North-Steppe Dnieper rivers. The article is devoted to the current state analysis of the higher aquatic vegetation at North-Steppe Dnieper lakes, its dynamics over a long-term period, as well as the determination of the nature and extent of anthropogenic-climatic changes in vegetation. Anthropogenic influence is a major threat to the development and functioning of most aquatic ecosystems. Since the twentieth century, it has been intensified by trends to long-term climate changes, which are also largely result of human activity. Increasing temperature of the winter season does not contribute to snow accumulation. Reduction of snow accumulation (frequent thaws during the winter), regulation of river flow (formation of a reservoirs cascade and ponds) and accumulation of melt water in artificial reservoirs led to the smoothing of the peak of the spring flood. Thus, the factor that provided spring washing of floodplain lakes, limited their overgrowing by air-water vegetation and their waterlogging disappeared. The anthropogenic factors that influence negatively include: intensification of agriculture, plowing of coastal areas, unreasonable land reclamation, overgrazing, development of transport and engineering infrastructure, urbanization, recreation, and chemical pollution. The presented data was obtained on the basis of processing our own research materials of 2009–2018 and literary and archival materials analysis (the herbarium of the Dnipropetrovs’k National University and the archive of the Research Institute of Biology). Natural Northern Steppe Dnieper lakes are located mainly in river valleys, so the study area was conventionally divided into sections: the large river valley (Dnieper) and the middle rivers valleys (Samara and Orel). Three ecological groups of macrophytes were reviewed and compared: hydatofites (submerged species), pleistophytes (species with floating leaves) and helophytes (air-water species). The vegetation of Dnieper floodplain lakes practically did not change for all three formation groups. The number of immersed plants communities within the floodplains of medium-sized rivers has decreased by three. The pleistophytes and helophytes associations decreased to fragments of associations. The lakes vegetation within the sandy Dnieper terrace practically did not change for all three formation groups. The submerged lakes plants associations within the sandy medium-sized rivers terraces have been reduced by two. As part of the lakes vegetation on the Dnipro saline terraces, fragments of associations of the two species are considered extinct. A new association of southern adventive species Ruppia maritima L. has appeared within the limits of the middle rivers saline terrace. Changes in higher aquatic vegetation are characteristic of all types of lakes. Changes occur in the direction of crowding out higher aquatic vegetation communities by airborne plant communities. The consequence of the anthropogenic-climatic transformation of aquatic ecosystems is increased mineralization, siltation, and, as a result, intensive overgrowing of lakes by aboriginal and adventive species with a wide ecological amplitude (replacement of sensitive to environmental changes species).


2020 ◽  
Vol 61 ◽  
pp. 77-85
Author(s):  
L. P. Chebannaya

The article presents the results of long-term research on the genus Clematis (Clematis L.) in the Stavropol Botanical garden. Biological, morphological and ornamental characteristics of varieties are provided; recommendations on selection of assortment and application in vertical gardening are given. For the first time, in the soil-climatic conditions of the Stavropol upland, an introduction study of 29 clematis varieties from six garden groups was conducted. According to the results of a comprehensive assessment, the most stable and highly decorative varieties were selected. In-depth study, parameters that have a significant value in the analysis of ornamental and economic-valuable qualities of varieties were revealed. In the course of research, when evaluating ornamental features, much attention was paid to the shape of the perianth, the size and color of the flowers. It was found that 24 varieties have an open flower shape, and five varieties have a bell – shaped one. The number of sepals varies from 4 to 6-8. In the presented garden groups, 27 varieties have a simple perianth, two with terry flowers. The size of the flower is divided into large-flowered, with a flower size of more than 10 cm and medium-flowered-from 4-10 cm. The color is varied, with many shades, from white-pink-red to blue-purple. When evaluating the prospects of the variety, special attention is paid to the duration and productivity of flowering. Varieties of the Lanuginosa and Patens groups bloom profusely in MayJune on shoots of the previous year and in July-September on annual shoots. In the groups Viticella, Jackmanii, Integrifolia and Texensis, abundant and prolonged flowering occurs on the shoots of the current year. Life forms determine not only the appearance of plants, but also are a criterion for use in garden and Park construction. Varieties of the Integrifolia group are preferably used for decorating low objects. For vertical gardening of structures with a height of more than three meters and as single and group planting varieties of garden groups are recommended Jackmanii, Lanuginosa, Patens, Viticella. The studied assortment, which has a complex of valuable ornamental and economic-biological features, is recommended for use in ornamental gardening and landscaping in the Stavropol territory.


2020 ◽  
Author(s):  
kanji tanaka

Although image change detection (ICD) methods provide good detection accuracy for many scenarios, most of the existing methods rely on place-specific background modeling. The time/space cost for such place-specific models becomes prohibitive for large-scale scenarios, such as long-term robotic visual simultaneous localization and mapping (SLAM). Therefore, we propose a novel ICD framework that is specifically tailored for long-term SLAM. This study is inspired by the multi-map-based SLAM framework, where N multiple localizers are capable of mutual diagnosis, thus not requiring any explicit background modeling/model. We extend this multi-map diagnosis approach toward a more generic single-map-based object-level diagnosis framework (i.e., ICD), where state-of-the-art self-localization systems can be used in their original form, which is as the change object indicator. The available single localizer is extended to different N localizers by introducing different N masked input images. Further, we also consider map diagnosis on a state-of-the-art deep-visual-SLAM system (rather than on conventional bag-of-words or landmark -based systems) in which the blackbox nature of the deep convolutional neural network (DCN) complicates the diagnosis problem. We also consider a 3D point cloud (PC) -based SLAM, and for the first time (to the best of our knowledge) adopt the state-of-the-art scan context PC descriptor for the purpose of map diagnosis.


1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)


2017 ◽  
pp. 34-47
Author(s):  
Hoi Le Quoc ◽  
Nam Pham Xuan ◽  
Tuan Nguyen Anh

The study was targeted at developing a methodology for constructing a macroeconomic performance index at a provincial level for the first time in Vietnam based on 4 groups of measurements: (i) Economic indicators; (ii) oriented economic indicators; (iii) socio-economic indicators; and (iv) economic - social – institutional indicators. Applying the methodology to the 2011 - 2015 empirical data of all provinces in Vietnam, the research shows that the socio-economic development strategy implemented by those provinces did not provide balanced outcomes between growth and social objectives, sustainability and inclusiveness. Many provinces focused on economic growth at the cost of structural change, equality and institutional transformation. In contrast, many provinces were successful in improving equality but not growth. Those facts threaten the long-term development objectives of the provinces.


Author(s):  
O. D. Golyaeva ◽  
O. V. Kurashev ◽  
S. D. Knyazev ◽  
А. Yu. Bakhotskaya

The main goal of the scientific institution was and remains to improve the assortment of fruit and berry crops for the development of domestic horticulture. Black currant breeding at VNIISPK was started by A.F Tamarova and continued by the doctor of agricultural Sciences T.P.Ogoltsova and doctor of agricultural Sciences S.D. Knyazev. A long-term breeding program has been developed. The main goals of the program are to create black currant cultivars with continuous resistance to diseases, first of all powdery mildew, as wells resistance to pests, i.e. bud mite. As a result of the long-term work, over 40 black currant cultivars have been developed, 14 of them are zoned. Red currant breeding was led by the candidate of agricultural Sciences L.V. Bayanova; since 2001 the work has been continued by the candidate of agricultural Sciences O.D. Golyaeva. ‘Heinemanns Rote Spӓtlese’, the descendant of R. multiflorum Kit., was involved in the red currant breeding for the first time in Russia. On its genetic basis, a series of late maturing cultivars with long and dense racemes was created. At the Institute, in total 21cultivars of red currants have been developed, 13 of them are zoned. At present, red currant cultivars make up 25.5% of the zoned assortment in Russia. The first research on gooseberries was stated by V.P. Semakin and A.F Tamarova; since 1992 the systematic gooseberry breeding has been carried out by the candidate of agricultural Sciences O.V. Kurashev. On the basis of Grossularia robusta, we have created gooseberry forms that are resistant to powdery mildew and leaf spots. These forms are highly productive, weakly thorned, having bush habit suitable for mechanized harvest. The result of breeding activities was the transfer of 6 gooseberry cultivars to State agricultural testing: ‘Solnechny Zaychik’, ‘Nekrasovsky’, ‘Yupiter’, ‘Zemlianichny’, ‘Moryachok’ and ‘Discovery’.


2004 ◽  
Vol 21 (3) ◽  
pp. 70-88 ◽  
Author(s):  
Nancy Gallagher

Public opinion in the United States and elsewhere celebrated the liberation of Afghan women following the defeat of the Taliban government. The United States promised to stay in Afghanistan and foster security, economic development, and human rights for all, especially women. After years of funding various anti- Soviet Mujahidin warlords, the United States had agreed to help reconstruct the country once before in 1992, when the Soviet-backed government fell, but had lost interest when the warlords began to fight among themselves. This time, however, it was going to be different. To date, however, conditions have not improved for most Afghan women and reconstruction has barely begun. How did this happen? This article explores media presentations of Afghan women and then compares them with recent reports from human rights organizations and other eyewitness accounts. It argues that the media depictions were built on earlier conceptions of Muslim societies and allowed us to adopt a romantic view that disguised or covered up the more complex historical context of Afghan history and American involvement in it. We allowed ourselves to believe that Afghans were exotic characters who were modernizing or progressing toward a western way of life, despite the temporary setback imposed by the Taliban government. In Afghanistan, however, there was a new trope: the feminist Afghan woman activist. Images of prominent Afghan women sans burqa were much favored by the mass media and American policymakers. The result, however, was not a new focus on funding feminist political organizations or making women’s rights a foreign policy priority; rather, it was an unwillingness to fulfill obligations incurred during decades of American-funded mujahidin warfare, to face the existence of deteriorating conditions for women, resumed opium cultivation, and a resurgent Taliban, or to commit to a multilateral approach that would bring in the funds and expertise needed to sustain a long-term process of reconstruction.


Crystals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 451
Author(s):  
Marta Kubiak ◽  
Janine Mayer ◽  
Ingo Kampen ◽  
Carsten Schilde ◽  
Rebekka Biedendieck

In biocatalytic processes, the use of free enzymes is often limited due to the lack of long-term stability and reusability. To counteract this, enzymes can be crystallized and then immobilized, generating cross-linked enzyme crystals (CLECs). As mechanical stability and activity of CLECs are crucial, different penicillin G acylases (PGAs) from Gram-positive organisms have proven to be promising candidates for industrial production of new semisynthetic antibiotics, which can be crystallized and cross-linked to characterize the resulting CLECs regarding their mechanical and catalytic properties. The greatest hardness and Young’s modulus determined by indentation with an atomic force microscope were observed for CLECs of Bacillus species FJAT-PGA CLECs (26 MPa/1450 MPa), followed by BmPGA (Priestia megaterium PGA, 23 MPa/1170 MPa) and BtPGA CLECs (Bacillus thermotolerans PGA, 11 MPa/614 MPa). In addition, FJAT- and BtPGA CLECs showed up to 20-fold higher volumetric activities compared to BmPGA CLECs. Correlation to structural characteristics indicated that a high solvent content and low number of cross-linking residues might lead to reduced stability. Furthermore, activity seems to be restricted by small water channels due to severe diffusion limitations. To the best of our knowledge, we show for the first time in this study that the entire process chain for the characterization of diverse industrially relevant enzymes can be performed at the microliter scale to discover the most important relationships and limitations.


Author(s):  
Kendrick Lee ◽  
Steven R. Laviolette ◽  
Daniel B. Hardy

Abstract Background Cannabis use in pregnancy leads to fetal growth restriction (FGR), but the long-term effects on cardiac function in the offspring are unknown, despite the fact that fetal growth deficits are associated with an increased risk of developing postnatal cardiovascular disease. We hypothesize that maternal exposure to Δ9-tetrahydrocannabinol (Δ9-THC) during pregnancy will impair fetal development, leading to cardiac dysfunction in the offspring. Methods Pregnant Wistar rats were randomly selected and administered 3 mg/kg of Δ9-THC or saline as a vehicle daily via intraperitoneal injection from gestational days 6 to 22, followed by echocardiogram analysis of cardiac function on offspring at postnatal days 1 and 21. Heart tissue was harvested from the offspring at 3 weeks for molecular analysis of cardiac remodelling. Results Exposure to Δ9-THC during pregnancy led to FGR with a significant decrease in heart-to-body weight ratios at birth. By 3 weeks, pups exhibited catch-up growth associated with significantly greater left ventricle anterior wall thickness with a decrease in cardiac output. Moreover, these Δ9-THC-exposed offsprings exhibited increased expression of collagen I and III, decreased matrix metallopeptidase-2 expression, and increased inactivation of glycogen synthase kinase-3β, all associated with cardiac remodelling. Conclusions Collectively, these data suggest that Δ9-THC-exposed FGR offspring undergo postnatal catch-up growth concomitant with cardiac remodelling and impaired cardiac function early in life. Impact To date, the long-term effects of perinatal Δ9-THC (the main psychoactive component) exposure on the cardiac function in the offspring remain unknown. We demonstrated, for the first time, that exposure to Δ9-THC alone during rat pregnancy results in significantly smaller hearts relative to body weight. These Δ9-THC-exposed offsprings exhibited postnatal catch-up growth concomitant with cardiac remodelling and impaired cardiac function. Given the increased popularity of cannabis use in pregnancy along with rising Δ9-THC concentrations, this study, for the first time, identifies the risk of perinatal Δ9-THC exposure on early postnatal cardiovascular health.


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