scholarly journals In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.)

2017 ◽  
Vol 2 (2) ◽  
pp. 37-42
Author(s):  
Samanhudi Samanhudi ◽  
Ahmad Yunus ◽  
Bambang Pujiasmanto ◽  
Anindya Saras
Keyword(s):  
In Vitro Propagation ◽  
Raw Materials ◽  
Root Number ◽  
Natural Habitats ◽  
Plant Propagation ◽  
Hair Roots ◽  
Number Of Leaves ◽  
Curcuma Xanthorrhiza ◽  
Propagation Technique

Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.

scholarly journals Effect of Cytokines On The In Vitro of Sweet Kaffir Lime (Citrus hystrix Dc)

2020 ◽  
Vol 3 (2) ◽  
pp. 60
Author(s):  
Rd. Selvy Handayani ◽  
Ismadi Yunus ◽  
Nadia Tillah ◽  
Ira Handayani
Keyword(s):  
Tissue Culture ◽  
Shoot Growth ◽  
Plant Propagation ◽  
Number Of Leaves ◽  
Local Plants ◽  
Propagation Technique ◽  
High Quality Seed ◽  
The Right ◽  
Culture Technology

Sweet kaffir lime (Citrus hystrix Dc), also called "Boh Kruet Mameh" is one of Aceh's local plants, which is currently very difficult to obtain. Sweet kaffir lime is not like the ordinary kaffir lime, which tastes sour. The fruit of sweet kaffir lime has a larger size than the regular kaffir lime, sweet and sour taste, aromatic and fresh, which can be consumed as a fruit table. The problem of developing the propagation technique of sweet kaffir lime plants is the seeds' condition, which are conventionally difficult to germinate and limited in number, making it difficult to get a high-quality seed. Thus, to overcome these problems, suitable solutions to plant propagation techniques should be sought. As for the right plant propagation technique is tissue culture technology or in vitro propagation technique. This research was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University. The research was conducted from February to April 2018. This research used a single randomized complete design (CRD) with ten replications. The treatment is a combination of the basic media, which consists of MS0 (MS media without BAP) and MS1 (MS + BAP 4 ppm). The result showed that the adduction of 4 ppm BAP in MS media influenced the in vitro sweet kaffir lime seeds' growth. Sweet kaffir lime seeds that were given 4 ppm BAP grew buds faster with more percentage of shoot growth, the number of shoots, and a number of leaves than the seeds without BAP adduction.

scholarly journals Culture medium for mint micropropagation in vitro

2020 ◽  
Author(s):  
N.A. Yegorova ◽  
◽  
M.S. Zagorskaya ◽  
O.V. Yakimova ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Medium Composition ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Second Stage ◽  
Clonal Micropropagation ◽  
Propagation Technique

The influence of the culture medium composition on the development of explants at the second stage of clonal micropropagation of mint (Mentha canadensis L. K59(4n)) was studied in order to improve the in vitro propagation technique. It was shown that the maximum multiplication rate (11.5) was provided by MS medium supplemented with BAP (1.0 mg/L), IAA (0.5 mg/L) and 2% sucrose.

scholarly journals PERTUMBUHAN KULTUR TUNAS AKSILAR KENTANG (Solanum tuberosum L.) DENGAN PENAMBAHAN SUPER FOSFAT DAN KNO3 PADA MEDIA AB MIX SECARA IN VITRO

2019 ◽  
Vol 20 (2) ◽  
pp. 71
Author(s):  
Hamami Alfasani Dewanto ◽  
Desi Saraswati ◽  
Oetami Dwi Hadjoeningtijas
Keyword(s):  
Culture Media ◽  
Raw Materials ◽  
Solanum Tuberosum L ◽  
Leaf Color ◽  
Tissue Culture Media ◽  
Randomized Design ◽  
Plantlet Growth ◽  
Number Of Leaves ◽  
Dark Green

Murashige&Skoog-based medium Potatoes are one commodity that has the potential to be developed as a resource in the context of food diversification, farmers' income riser, non fossil export commodities and raw materials for processing industry. The objective of this research was to find out the effect of SP-36 fertilizer, KNO3 fertilizer, as well as the interaction between the two fertilizers on the growth of potato nodal culture on AB Mix media in vitro. The results of this study are expected to provide economical potato tissue culture media development. This research used factorial complete randomized design. The treatment were SP-36 concentration: 0 ppm; 50 ppm; 100 ppm; and 200 ppm, in combination with KNO3 concentration: 0 ppm; 100 ppm; 200 ppm; 400 ppm; and 600 ppm, The variables observed included number of leaves, leaf color, length of plantlets, fresh weight of plantlets and percentage of plantlets growth. Based on the results of the analysis of variance (ANOVA) F. Calculate < F. Table with the average success of plantlet growth between 87.5-100%. In addition, there are four types of leaf color produced, namely the color of yellowish green, pale green leaves, green, and dark green. Research showed that the interaction between SP-36 fertilizer and KNO3 fertilizer on AB Mix media had no significant effect on all observed variables.

scholarly journals 606 In Vitro Propagation of Some Strawberry Cultivars in Response to Kind and Concentration of Some Cytokinin Compounds

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 501D-501
Author(s):  
M.H. Aboul-Nasr ◽  
S.Z. Elagamy ◽  
A.M. Kassim
Keyword(s):  
In Vitro Propagation ◽  
Callus Formation ◽  
Shoot Length ◽  
Control Treatment ◽  
Fresh Weight ◽  
Shoot Height ◽  
Murashige And Skoog Medium ◽  
Number Of Leaves ◽  
Strawberry Cultivars

Three strawberry cultivars were used in this study. Runners from the three cultivars were grown on Murashige and Skoog medium supplemented with benzylaminpurine or kinetin at four concentrations (2, 1, 0.5, and 0.25 mg·L-1 in addition to the control treatment). The use of cytokinin, regardless of type and concentration, promoted the explant proliferation, shoot height, number of leaves, and plantlet fresh weight. Callus formation was enhanced by BA application, especially with `Pajaro'. However, `Chandler' did not form at all using cytokinin. Produced plantlets had lower nutrient constituents (N, P, K, Ca, and Na) when grown on media containing cytokinin compared to the control. Based on these results, cytokinin is recommended to get more shoots per explant. Furthermore, using the lowest concentration (0.25 mg·L-1) will produce height proliferation, greater shoot length, and more leaves.

scholarly journals Acclimatization of Eurycoma longifolia (Tongkat Ali) Plantlets to Ex Vitro Conditions

2021 ◽  
Vol 3 (1) ◽  
pp. 129-131
Author(s):  
Muhammad Fuad Yahya ◽  
Nor Hasnida Hassan ◽  
Nazirah Abdullah ◽  
Siti Suhaila Abd. Rahman ◽  
Haliza Ismail ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Ex Vitro ◽  
Soil Mixture ◽  
Eurycoma Longifolia ◽  
Planting Material ◽  
Top Soil ◽  
Herbal Plants ◽  
Potting Media ◽  
Propagation Technique

Eurycoma longifolia is one of the famous herbal plants with great medicinal benefits. The plant which also known as Tongkat Ali is well-known for treating erectile dysfunction and as energy booster. Tongkat Ali plantlets were produced using in vitro propagation technique to cater the demand for planting material. The development of successful acclimatization technique is prerequisite for in vitro propagation method. Acclimatization is a step to prepare the plantlets to survive and grow in different environment compare to laboratory. In this study, an attempt was made to identify the best potting media to acclimatize Tongkat Ali using glass chamber. Rooted plantlets about 2-4 cm height were used and subsequently transferred to different potting media for acclimatization i.e jiffy 7, sand, baked soil, mixture of cocoa peat and sand, sand and top soil and lastly cocoa peat and baked soil. Plantlets grown in jiffy 7 showed 100% survival followed by the mixture of cocoa peat and baked soil with 94.45% of survival. The acclimatized plantlets were transplanted into polybag and maintained in the shadehouse condition.

Perbandingan Metode Sterilisasi Untuk Perbanyakan Rubus rosifolius Secara in Vitro

2021 ◽  
Vol 14 (1) ◽  
pp. 127-137
Author(s):  
Muhammad Imam Surya ◽  
Lily Ismaini
Keyword(s):  
Tissue Culture ◽  
Ascorbic Acid ◽  
In Vitro Propagation ◽  
Povidone Iodine ◽  
Tween 80 ◽  
Botanical Garden ◽  
The Other ◽  
Fruit Crops ◽  
Plant Propagation

AbstrakRubus rosifolius adalah salah satu jenis rasberi liar yang memiliki potensi cukup tinggi untuk dikembangkan sebagai tanaman buah. Selain itu, metode perbanyakan tanaman merupakan salah satu faktor yang berpengaruh dalam pembudidayaan. Lebih lanjut, informasi terkait upaya perbanyakan R. rosifolius secara in vitro masih sangat terbatas. Percobaan ini ditujukan untuk mengetahui metode sterilisasi yang tepat pada eksplan R. rosifolius. Sebanyak 17 metode sterilisasi telah diujicobakan di Laboratorium Kultur Jaringan BKT Kebun Raya Cibodas-LIPI. Bahan sterilisasi yang digunakan, yaitu detergen, tween 80, bakterisida, fungisida, clorox/pemutih (NaClO), alkohol 70%, larutan Plant Preservative Mixture (PPM), vitamin C/asam askorbat, dan povidone iodine/antiseptik. Hasil percobaan menunjukkan bahwa metode sembilan merupakan metode sterilisasi yang cukup optimum untuk sterilisasi eksplan R. rosifolius. Metode sembilan mampu menghambat munculnya mikroorganisme endofitik hingga 8 hari dan tidak menyebabkan warna eksplan menjadi cokelat/browning. Tahapan sterilisasi pada metode sembilan meliputi pencucian dengan detergen, perendaman dengan bakterisida + fungisida selama +30 menit, perendaman dengan clorox 10% + tween 80 selama +15 menit, pencucian dengan larutan PPM selama +15 menit.  AbstractRubus rosifolius is one of the species from wild raspberries, which is has high potential to develop as a fruit crops. In the other hand, the technique of plant propagation became an important factor for cultivation. Moreover, the information related to the in vitro propagation of R. rosifolius is very limited. This experiment was aimed to determine the best method to sterilize an explants of R. rosifolius. About 17 methods of sterilization have been tried in the laboratorium of tissue culture at Cibodas Botanical Garden-Indonesian Institute of Sciences. The combination of detergent, tween 80, bactericide, fungicide, sodium hypochlorite (NaClO), alcohol 70%, plant preservative mixture (PPM), ascorbic acid, and povidone iodine were used during the experiment. The results show that the method of sterilization number nine could be inhibit the emergence of endophytic organisms for eight days and keep an explant in green with a little brownish compared by the others methods. The method of sterilization number nine was consist of several steps i.e. wash by detergent, soak in bactericide + fungicide for +30 minutes, soak in sodium hypoclorite 10% + tween 80 for +15 minutes, wash by PPM solution for +15 minutes.

In vitro Propagation of Two Grapevine (Vitis vinifera L.) Cultivars under Conditions of Salt Stress

2020 ◽  
Vol 30 (1) ◽  
pp. 47-56
Author(s):  
Getachew Kassa ◽  
Tileye Feyissa
Keyword(s):  
Salt Stress ◽  
In Vitro Propagation ◽  
Dry Weight ◽  
Vitis Vinifera L ◽  
Saline Soils ◽  
Free Medium ◽  
Single Node ◽  
Rooting Medium ◽  
Number Of Leaves

This study was aimed to investigate salt tolerance of two grapevine cultivars, ‘Chenine Blanc’ and ‘Canonannon’ through in vitro propagation on medium containing different concentrations of NaCl. Single-node shoots were cultured on MS with 1.0 mg/l BAP in combination with 0.1 mg/l IBA and containing 0.25, 0.50, 0.75, 1.00 or 1.50% NaCl. NaCl free medium was used as control. Shoots of both cultivars were cultured on the same MS containing 0.25, 0.50, 0.75 or 1.00% CaCl2 to reduce hyperhydricity. The shoots were transferred to rooting medium followed by acclimatization in greenhouse. Number and length of shoots and roots, number of leaves and nodes, length of nodes, fresh and dry weight of roots and shoots decreased significantly in consistent trend as the concentration of NaCl increased. ‘Canonannon’ cultivar was found to be significantly more tolerant to NaCl than ‘Chenine Blanc’ in all parameters. The lowest percentage of hyperhydric shoots were obtained on medium containing 0.25% CaCl2. Therefore, ‘Canonannon’ cultivar can be planted in relatively saline soils as it is more tolerant to salt than ‘Chenine Blanc’. Plant Tissue Cult. & Biotech. 30(1): 47-56, 2020 (June)

scholarly journals In vitro Propagation of Noni (Morinda citrifolia L.) Through Embryo Culture

2020 ◽  
Vol 30 (2) ◽  
pp. 199-207
Author(s):  
JO Afolabi ◽  
EO Oloyede ◽  
AO Akala
Keyword(s):  
Embryo Culture ◽  
In Vitro Propagation ◽  
Zygotic Embryo ◽  
Shoot Length ◽  
Morinda Citrifolia ◽  
Distilled Water ◽  
Number Of Leaves ◽  
And Control ◽  
Mature Zygotic Embryo

The chances of shoot-regeneration from embryo culture of Morinda citrifolia L. seeds was investigated. Germination on different strengths of MS and control (Sterile distilled water) started by two weeks after inoculation (WAI). At 6 WAI, 90% of the embryo had germinated from 25% MS followed by 80% in control, 70% from 50% MS and 40% each from 100 and 75% MS. Similarly, the same MS media strengths with basal application of 2.0/1.0 mg/l BAP/Kn affected the growth of regenerated Noni-plantlets. The longest shoot length (3.46 cm) and the number of nodes (1.75) were obtained from 75% MS while the highest number of leaves (7.25) was obtained in 100% MS between 4 and 12 WAI. The lowest value for these parameters were observed in 25% MS. This showed that mature zygotic embryo is good explant for the establishment of highly viable and re-generable plantlets of Noni. Plant Tissue Cult. & Biotech. 30(2): 199-207, 2020 (December)

scholarly journals Micro Propagation of Several Potted Anthurium Accessions Using Spathe Explants

2012 ◽  
Vol 11 (1) ◽  
pp. 59 ◽  
Author(s):  
Kurniawan Budiarto
Keyword(s):  
In Vitro Propagation ◽  
Light Conditions ◽  
Defined Media ◽  
Micro Propagation ◽  
New Varieties ◽  
The Media ◽  
Ornamental Crops ◽  
Propagation Technique ◽  
Genotypic Alteration

The introduction of new varieties and production system of anthuriums has faced some problems due to the lowmultiplication rates in conventional vegetative and genotypic alteration problem in reproductive propagation. Sincethen, in vitro propagation technique became important to be investigated. The research was carried out fromSeptember 2006 to August 2007 at the Indonesian Ornamental Crops Research Institute. A complete factorialexperiment was designed to accomplish two chronological in vitro activities. The first step dealt with differentincubation sites, i.e. dark and light conditions for callus induction of three potted anthurium accessions, namelyclone no. SM. 001, cv. Alphine and cv. Bonito. The best callus obtained from the incubation treatment were then,transferred into defined media with different formulations to get free-callus plantlets in the second steps. Theresults showed that more progressive callus development from spathe explant was detected on the media ofmodified Nitsch and Nitsch + 1 mg/l 2,D + 1 mg/l Kin + 1 mg/l BA stored at dark after 60 days incubation. After 45days transferring the callus in the same media on light conditions, torpedo-shaped callus was detected. Completeplanlets were obtained after 75 days reculturing the buds in Nitsch and Nitsch medium containing 2,4 D withvarious BA concentrations. Clone SM. 001 and cv. Bonito showed higher number of visible shoot and rootdevelopments compared to cv. Alphine. In general, better shoot and root developments and higher planlets survivedafter acclimatization were observed on the BA concentrations of 2 and 3 mg/l.

scholarly journals In vitro Propagation of Bacopa monnieri (L.)

2020 ◽  
pp. 32-39
Author(s):  
Poornima Raj ◽  
J. Anbumalarmathi ◽  
S. Aruna Sharmili
Keyword(s):  
Survival Rate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Bacopa Monnieri ◽  
Shoot Length ◽  
Ms Medium ◽  
Root Initiation ◽  
Shoot Induction ◽  
Propagation Technique

An experiment was conducted for standardization of in vitro propagation technique of Bacopa monnieri (L.), a medicinal herb of India. Healthy leaf segments of the herb were used as explants with basic Murashige and Skoog (MS) medium containing various combinations of different growth regulators for callus, shoot and root initiation. The best callus induction percentage (95.47%) was observed on MS + 0.5 mg/L NAA and 2.0 mg/L BAP (T3). The maximum number of shoots (8), shoot length (9.30 cm) and shoot induction percentage (90.48%) was achieved on MS + 3.0 mg/L BAP and 1.0 mg/L Kn (ST4). The maximum number of roots (8) and root length (7) was observed on MS + 1.5 mg/L IAA (RT5). The rooted micro shoots were successfully hardened and acclimatized in green house and subsequently established in soil with survival rate of 90%.

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