scholarly journals A Novel Fluorimetric Method to Evaluate Red Wine Antioxidant Activity

2018 ◽  
Vol 63 (1) ◽  
pp. 57-64
Author(s):  
Franco Tubaro ◽  
Roberto Pizzuto ◽  
Gennaro Raimo ◽  
Gianluca Paventi
Keyword(s):  
Antioxidant Activity ◽  
Red Wine ◽  
Antioxidant Properties ◽  
Fast Method ◽  
Red Wines ◽  
Polyphenol Content ◽  
Fluorimetric Method ◽  
Bleaching Test ◽  
Cheap Method ◽  
Wine Polyphenol

In the light of the increasing use of red wine in the diet, due to its antioxidant properties, the availability of a simple and fast method to assay the antioxidant activity of this beverage is needed. Here we measured wine antioxidant capacity (WAC) via "crocin bleaching test", wine polyphenol content (WPC), via Folin Ciocalteu method, and fluorimetric response (WF) of 13 Italian red wines. A fairly good correlation between WAC and WF (R = 0.82) was found, thus validating the fluorimetric method as a simple, fast and cheap method to evaluate the antioxidant activity of red wines.

scholarly journals Influence of different commercial fining agents on proanthocyanidin fraction and antioxidant activity of a red wine from Baga grapes

OENO One ◽  
2009 ◽  
Vol 43 (2) ◽  
pp. 111
Author(s):  
Fernando Jorge Gonçalves ◽  
António Manuel Jordão
Keyword(s):  
Antioxidant Activity ◽  
Red Wine ◽  
Antioxidant Properties ◽  
Human Organism ◽  
Red Wines ◽  
Antioxidant Power ◽  
Egg Albumin ◽  
Degree Of Polymerisation ◽  
Fining Agents ◽  
The Impact

<p style="text-align: justify;"><strong>Aims</strong>: Evaluate the impact of different commercial fining agents (egg albumin, bentonite, gelatine, potassium caseinate and isinglass) and their concentration on proanthocyanidin fraction content (monomeric, oligomeric and polymeric) and antioxidant activity of a red wine from Baga grape variety. Additionally establishment of correlations between the antioxidant activity and the different proanthocyanidin fraction content were also an other aim of this work.</p><p style="text-align: justify;"><strong>Methods and results</strong>: The fractionation of wine proanthocyanidins was made according to their polymerisation degree: catechins (monomers), oligomeric (degree of polymerisation ranging from 2 to 12-15) and polymeric (degree of polymerisation &gt; 12-15). For antioxidant activity of red wine, two analytical methods were used: the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ABTS radical cation scavenging assay. In general the different commercial fining agents applied induced a decrease of the wine total phenols and the different proanthocyanidin fraction content studied. The monomeric flavanols were significantly depleted by egg albumin and isinglass (specially using the highest concentration), while for oligomeric fraction only the highest egg albumin, gelatine and isinglass concentration induced a decrease on the values.</p><p style="text-align: justify;"><strong>Conclusion</strong>: The commercial fining agents analyzed in the present study affected the monomeric and the oligomeric proanthocyanidin. At same time some fining agents (specially in high concentrations), such as egg albumin, isinglass and gelatine induced a reduction of antioxidant activity of red wines too.</p><p style="text-align: justify;"><strong>Significant and impact study</strong>: The knowledge of the impact of the commercial fining agents in proanthocyanidin content and antioxidant properties of red wines is important for red wine fining optimisation and consequently for the red wine quality. This study is particular important when the « antioxidant power » of the foods, in this case in red wine, is an expression of its capability both to defend the human organism from the action of the free radicals and to prevent degenerative disorders deriving from persistent oxidative stress.</p>

scholarly journals ANTIOXIDANT PROPERTIES EVALUATION FOR DIFFERENT TYPES OF APPLE VINEGAR WITH UNALCOHOLIC RED WINE CONCENTRATES ADDITION

1970 ◽  
Vol 63 ◽  
Author(s):  
Mariana Atena Poiană ◽  
I. Gergen ◽  
Diana Moigrădean ◽  
Viorica Târu ◽  
Diana Dogaru
Keyword(s):  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Total Antioxidant Capacity ◽  
Red Wine ◽  
Antioxidant Properties ◽  
Differential Method ◽  
Cabernet Sauvignon ◽  
Red Wines ◽  
Total Antioxidant ◽  
Monomeric Anthocyanins

In this paper it was obtained the apple vinegar with addition of red wines concentrates in different percents for to improve the antioxidant properties. For processing of red wine concentrates it was used the young red wines Merlot and Cabernet Sauvignon. For resulted vinegar types were analyzed total acidity, extract, total antioxidant capacity (using FRAP method), total polyphenols amount (by Folin-Ciocalteu method) and monomeric anthocyanins (using pH-differential method). Polyphenols content from vinegar with Cabernet Sauvignon concentrates addition was situated in the range 0.74-3.42 mM gallic acid/L and for vinegar with red wine Merlot concentrates addition between 0.74-2.64 mM gallic acid/L. The antioxidant capacity was presented the values between 0.45-8.18 mM Fe2+/L for apple vinegar with Cabernet Sauvignon concentrates addition and between 0.45-6.69 mM Fe2+/L for vinegar with Merlot concentrate addition. The polyphenols content and monomeric anthocyanins content of apple vinegars with red wine concentrates increase in rapport with the percent of red wines concentrates added. The values of polyphenols content and total antioxidant capacity were more with approximate 20% in the case of vinegar with Cabernet Sauvignon concentrates addition comparatively with the case of vinegar with Merlot concentrates addition.

scholarly journals Wines with Increased Lignan Content by the Addition of Lignan Extracts

2016 ◽  
Vol 34 (No. 5) ◽  
pp. 439-444 ◽  
Author(s):  
Balík Josef ◽  
Híc Pavel ◽  
Kulichová Jana ◽  
Novotná Pavla ◽  
Tříska Jan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Storage Time ◽  
Red Wine ◽  
White Wine ◽  
Polyphenol Content ◽  
Total Polyphenols ◽  
Total Polyphenol ◽  
Ethanol Extracts ◽  
One Year ◽  
And Storage

Red and white wines [Grüner Veltliner white wine and Blue Limberger (Blaufränkisch) red wine (vintage 2013)] were enriched with lignan hydroxymatairesinol originated from spruce knots. These spruce knots with removed resin were extracted with ethyl alcohol of agricultural origin. Ethanol extracts of lignans were then used to enrich wine sorts. Enriched wines were stored for 13 months. At 2, 6, and 13 months, samples were taken and subjected to a variety of analyses and sensory evaluations. Analyses included 7-hydroxymatairesinol and alpha-conidendrin lignan content, antioxidant activity (as determined by FRAP), total polyphenols, and sensory evaluation. The obtained data were evaluated using the analysis of variance to determine which factors e.g. wine type, quantity of added lignan extracts, additional sugar, method of preservation, and storage time had the most significant influence on lignan content, antioxidant activity and polyphenol content. In all cases the lignan content in the wines was significantly influenced by the addition of lignan extracts. After one year of storage, lignan contents changed only moderately and added lignans were stable in stored wines. Total polyphenol content in wines and the antioxidant activity of wines were significantly influenced by the type of wine (i.e., red or white). The presented method of wine enrichment with lignans opens the door for the production of extra quality wines.

scholarly journals Evaluation of the antioxidant activity of some types of red and white wines

2014 ◽  
Vol 25 (2) ◽  
pp. 65-70 ◽  
Author(s):  
Ana Leahu ◽  
Sonia Amariei ◽  
Cristina Damian ◽  
Mircea Oroian ◽  
Sorina Ropciuc
Keyword(s):  
Antioxidant Activity ◽  
Multivariate Data Analysis ◽  
Red Wine ◽  
Total Phenolics ◽  
Principal Component ◽  
White Wine ◽  
Total Phenols ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
White Wines

Abstract Samples of commercially available Romanian wines were analyzed in order to determine total phenols content and the antioxidant activity. The content of total phenolics in the extracts was determined according to the Folin-Ciocalteu method and calculated as gallic acid equivalents (mg GAE/100g). Antiradical activities of the extracts were evaluated by a micro assay using 1, 1¢-diphenyl-2-picrylhydrazyl spectrophotometric method. Wine characteristics measurements were examined by multivariate data analysis, using principal component analysis (PCA). Total polyphenol content was correlated to the antioxidant activity of the studied wine samples. The values of the inhibition power of free radical, PI%, are ranging between 1.68 for white wine and 0.95 for red wine (“Bull blood” bottled by Tohani winery, Prahova, Romania).

scholarly journals Changes of antioxidant activity and active compounds content in selected teas

2020 ◽  
Vol 8 (1) ◽  
pp. 91-97
Author(s):  
Barbara Kolodziej ◽  
Sugier Danuta ◽  
Luchowska Katarzyna
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Ferric Ions ◽  
Polyphenol Content ◽  
Total Polyphenols ◽  
Total Polyphenol ◽  
Total Polyphenol Content ◽  
Tea Infusions ◽  
Anti Oxidant ◽  
Brewing Time

Our study tested 45 tea infusions classified into five groups (white, green, red, black, and other teas) for the content of total polyphenols and flavonoids, as well as antioxidant properties, by the FRAP and DPPH methods. We examined these parameters after prolongation of the brewing time from 10 to 30 min and overnight storage. The results showed that the capacity of the teas to bind free radicals was differentiated and the amount of anti-oxidant compounds depended on their nature. In terms of antioxidant activity and total polyphenol content, the tested tea types were ranked in the following order: white > green > black > red > other teas (yerba mate > rooibos). Our experiment demonstrated a positive correlation between the polyphenol content and antioxidant activity of the analyzed teas. Also, the DPPH antiradical efficiency was comparable to their ability to reduce ferric ions. The extended brewing time had a significant effect on the antioxidant activity of the infusions and the polyphenolic compounds analyzed therein. In contrast, storage of the infusions for 24 h at room temperature changed their antioxidant activity and affected the total polyphenol content.

scholarly journals Chemical Composition, Antioxidant Properties and Anti-cholinesterase Activity of Cordia gilletii (Boraginaceae) Leaves Essential Oil

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Marco Bonesi ◽  
Philippe N. Okusa ◽  
Rosa Tundis ◽  
Monica R. Loizzo ◽  
Federica Menichini ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Properties ◽  
In Vitro Tests ◽  
Bleaching Test ◽  
Total Oil ◽  
Β Carotene ◽  
Terpene Derivatives

This study aimed to investigate for the first time the chemical composition, the antioxidant properties and the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity of the essential oil from the leaves of Cordia gilletii De Wild (Boraginaceae). The essential oil, characterized by 23 constituents (90.1% of the total oil), was constituted by terpene derivatives (25.6%) and non-terpene derivatives (64.5%), among which aldehydes, fatty acids and alkanes were present with the percentage of 16.5%, 18.8% and 23.1%, respectively. The antioxidant activity of C. gilletii essential oil was screened by two in vitro tests: DPPH and β-carotene bleaching test. The essential oil revealed antioxidant activity with an IC50 value of 75.0 and 129.9 μg/mL on DPPH radical and β-carotene decoloration tests, respectively. Moreover, C. gilletii inhibited AChE enzyme with an IC50 value of 105.6 μg/mL.

scholarly journals Potential of Cooperage Byproducts Rich in Ellagitannins to Improve the Antioxidant Activity and Color Expression of Red Wine Anthocyanins

Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 336 ◽  
Author(s):  
María Luisa Escudero-Gilete ◽  
Dolores Hernanz ◽  
Celia Galán-Lorente ◽  
Francisco J. Heredia ◽  
María José Jara-Palacios
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Red Wine ◽  
Model Solution ◽  
Red Wines ◽  
Liquid Chromatography Mass Spectrometry ◽  
Wood Shavings ◽  
Technology Applications ◽  
Antioxidant Characteristics

Cooperage byproducts are an important source of phenolic compounds that could be used for wine technology applications. The effects of the addition of two types of oak wood shavings (American, AOW, and Ukrainian, UOW) on the antioxidant activity and color of red wine anthocyanins, in a wine model solution, were evaluated by spectrophotometric and colorimetric analyses. Phenolic compounds from shavings, mainly ellagitannins, were determined by ultra-high-performance liquid chromatography/mass spectrometry (UHPLC/MS). Antioxidant and copigmentation effects varied depending on the type of shavings (AOW and UOW) and the phenolic concentration (100, 400, and 500 mg/L). Phenolic compounds from shavings improved the color characteristics (darker and more bluish color) and the copigmentation effect of red wine anthocyanins, being UOW a better source of copigments than AOW shavings. The best antioxidant activity was found for the 400 and 500 mg/L model solutions for both types of shavings. Results show a winemaking technological application based on the repurposing of cooperage byproducts, which could improve color and antioxidant characteristics of red wines.

scholarly journals Comparative Analysis Regarding the Chemical and Microbiological Characteristics of Some Red Wine Assortments Produced in Two Romanian Viticultural Areas

2020 ◽  
Vol 71 (1) ◽  
pp. 411-415
Author(s):  
Thomas Dippong ◽  
Cristina Mihali ◽  
Zorica Vosgan ◽  
Alexandra Avram ◽  
Zoita Berinde ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Comparative Analysis ◽  
Metal Content ◽  
Red Wine ◽  
Chemical Properties ◽  
Total Acidity ◽  
Red Wines ◽  
Polyphenol Content ◽  
Microbiological Characteristics ◽  
Sweet Wine

Sweet, semi-dry and semi-sweet red wine samples from two vine-growing regions in Romania: Vrancea and Recas were analyzed aiming to show their main chemical properties including heavy metal content, and, microbiological characteristics. The chemical analysis showed that the semi-dry wine (W1) has a high polyphenol content, permanganate index and content of Fe, Zn and Cu, but the lowest content of anthocyanins, sugar and Mn; the total acidity of semi-sweet wine W3 was the highest, while the semi-sweet wine W2 contained the highest concentrations of anthocyanins and SO2. No contamination with yeasts, molds and bacteria was found when opening the wine bottles, these colonies having grown one month after opening. The importance of this article comes from the comprehensive presentation of the connections among the physicochemical and microbiological characteristics of sweet to semi-sweet red wines, or to semi-dry from two vine-growing regions in Romania: Vrancea and Recas.

scholarly journals Grapes, Wines and By-products as Potential Sources of Antioxidants

1995 ◽  
Author(s):  
Joseph Kanner ◽  
Edwin Frankel ◽  
Stella Harel ◽  
Bruce German
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Vitamin E ◽  
Phenolic Compounds ◽  
Red Wine ◽  
Membrane Lipid ◽  
Membrane Lipid Peroxidation ◽  
Red Wines ◽  
High Concentration ◽  
By Products

Several grape varieties and red wines were found to contain large concentration of phenolic compounds which work as antioxidant in-vitro and in-vivo. Wastes from wine production contain antioxidants in large amounts, between 2-6% on dry material basis. Red wines but also white wines were found to prevent lipid peroxidation of turkey muscle tissues stored at 5oC. The antioxidant reaction of flavonoids found in red wines against lipid peroxidation were found to depend on the structure of the molecule. Red wine flavonoids containing an orthodihydroxy structure around the B ring were found highly active against LDL and membrane lipid peroxidation. The antioxidant activity of red wine polyphenols were also found to be dependent on the catalyzer used. In the presence of H2O2-activated myoglobin, the inhibition efficiency was malvidin 3-glucoside>catechin>malvidin>resveratol. However, in the presence of an iron redox cycle catalyzer, the order of effectiveness was resveratol>malvidin 3-glucoside = malvidin>catechin. Differences in protein binding were found to affect antioxidant activity in inhibiting LDL oxidation. A model protein such as BSA, was investigated on the antioxidant activity of phenolic compounds, grape extracts, and red wines in a lecithin-liposome model system. Ferulic acid followed by malvidin and rutin were the most efficient in inhibiting both lipid and protein oxidation. Catechin, a flavonal found in red-wines in relatively high concentration was found to inhibit myoglobin catalyzed linoleate membrane lipid peroxidation at a relatively very low concentration. This effect was studied by the determination of the by-products generated from linoleate during oxidation. The study showed that hydroperoxides are catalytically broken down, not to an alcohol but most probably to a non-radical adduct. The ability of wine-phenolics to reduce iron and from complexes with metals were also demonstrated. Low concentration of wine phenolics were found to inhibit lipoxygenase type II activity. An attempt to understand the bioavailability in humans of antocyanins from red wine showed that two antocyanins from red wine were found unchanged in human urine. Other antocyanins seems to undergo molecular modification. In hypercholesterolemic hamsters, aortic lipid deposition was significantly less in animals fed diets supplemented with either catechin or vitamin E. The rate of LDL accumulation in the carotid arteries was also significantly lower in the catechin and vitamin E animal groups. These results suggested a novel mechanism by which wine phenolics are associated with decreased risk of coronary heart diseases. This study proves in part our hypothesis that the "French Paradox" could be explained by the action of the antioxidant effects of phenolic compounds found at high concentration in red wines. The results of this study argue that it is in the interest of public health to increase the consumption of dietary plant falvonoids. Our results and these from others, show that the consumption of red wine or plant derived polyphenolics can change the antioxidant tone of animal and human plasma and its isolated components towards oxidative reactions. However, we need more research to better understand bioavailability and the mechanism of how polyphenolics affect health and disease.

scholarly journals Quality Characteristics and Antioxidant Activity of Yogurt Containing Raw Omija and Sugared Omija during Storage

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Won-Young Cho ◽  
Sung-Hyun Hwa ◽  
Fengqi Yang ◽  
Chi-Ho Lee
Keyword(s):  
Antioxidant Activity ◽  
Sensory Evaluation ◽  
Antioxidant Properties ◽  
Storage Period ◽  
Titratable Acidity ◽  
Quality Characteristics ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Chelating Activity ◽  
Dpph Activity

This study was conducted to determine the quality characteristics and antioxidant properties of yogurt containing omija extract (control, raw omija, and sugared omija) stored at 4°C for 14 days. The pH of all groups decreased, while the titratable acidity increased as the storage period increased. The viscosity of the sugared omija sample was high, while in the syneresis test, the sugared omija sample showed a low value. The total polyphenol content was the highest in the raw omija sample on day 0. DPPH activity was the highest in the raw omija sample for all storage periods; this sample also showed high Fe2+ chelating activity, which did not significantly differ from the sugared omija sample. In sensory evaluation, the sugared omija sample showed the highest overall score. Based on these results, it can be concluded that yogurt containing sugared omija shows improved quality and antioxidant activity.

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