scholarly journals Tissue preparations as an eff ective means of correction of sexual cyclicalityof infertile cows in the conditions of forest-steppe zone in Zhytomyr region

Author(s):  
A. Revunets ◽  
G. Gryshchuk ◽  
Ya. Veremchuk

Implementation of the latest technologies of animal reproduction causes changes in the life of their body and infl uences the manifestation of sexual cyclicity. As a result, one of the main tasks of specialists of veterinary medicine is the elimination of infertility of cows and heifers and improvement of methods of regulation of their reproductive functions. In recent years, in veterinary medicine for prophylactic and therapeutic purposes individually or in combination with other agents during pregnancy, in the postpartum period for gynecological diseases, to stimulate the functions of the sexual apparatus of animals, and their farming to improve productivity eff ectively used biogenic stimulators, that are made of tissues and organs of animals and plants – tissue preparations. But remain changes of the sexual apparatus of infertile cows are not studied in their use. Therefore, the purpose of the research was to study the eff ect of tissue preparations in a complex with ihglukovit for the correction of the sexual cycle of the infertile cows in the conditions of forest-steppe in Zhytomyr region. It has been found that the using tissue preparations make from the liver and placenta and ihglukovit for cows, time successful insemination was minimal and was 47.0±5.11 days. We also observed a positive eff ect on the usefulness of the fl ow of the sexual cycle when used in complex tissue preparations from the liver and placenta, as a consequence of infertility in the cow was of 17.00±1.14 days. As all experienced animals received ihglukovit, and the period of occurrence of complete sexual cycle and the eff ectiveness of insemination of cows was diff erent, then we assume that the changes in animals are due primarily to the infl uence of tissue preparations. We believe that the using of "placental" tissue preparation make from the uterus of pregnant cows with contents was the most eff ective, because it includes biologically active substances that are similar to the sexual apparatus of a cow. Analyzing the obtained results, we recommend to apply the tissue preparation liver, placenta and ihglukovit at a dose of 20 cm3 per animal, three times with an interval of 8-10 days to correct the sexual cycle of cattle and prevention of infertility. Key words: biogenic stimulant, tissue preparation, ihglukovit, reproduction, sexual cycle, infertility, cows.

2019 ◽  
pp. 177-184
Author(s):  
Tat'yana Ivanovna Fomina ◽  
Tat'yana Abdulkhailovna Kukushkina

The results of comparative study of the content of biologically active substances in the aboveground part of 11 species of perennial onions Allium aflatunense B. Fedtsch., A. altaicum Pall., A. flavum L., A. microdictyon Prokh., A. nutans L., A obliquum L., A. ramosum L., A. rosenbachianum Regel, A. schoenoprasum L., A. senescens L. var. glaucum Regel (A. senescens ssp. glaucum (Schrad.) N. Friesen), A. strictum Schrad. are presented. The freshly collected raw materials in the phase of consumer ripeness were analyzed and all indicators, except for the amount of ascorbic acid, were calculated on the mass of absolutely dry raw materials. It was established in onions a high content of dry substances (up to 25.1%), flavonols (up to 3.4%), tannins (up to 14.6%), pectin substances (up to 25.8%), sugars (up to 34.8%), ascorbic acid (up to 222.5 mg% ), carotenoids (up to 131.9 mg%); the content of catechins was insignificant, at the level of 0.04–0.15%, only in A. rosenbachianum – up to 0.56%. The contents of catechins, tannins, pectin substances in onions were determined for the first time. A high interspecific and individual variability of accumulation of biologically active substances was revealed. A. rosenbachianum, A. aflatunense, A. flavum, A. microdictyon have the greatest contents and relatively low indicators are in A. senescens var. glaucum. The species are promising for cultivation in the forest-steppe zone of Western Siberia as early vitamin-bearing plants and as a source of various biologically active compounds.


2017 ◽  
pp. 89-96 ◽  
Author(s):  
Ирина (Irina) Георгиевна (Georgievna) Боярских (Boyarskikh) ◽  
Владимир (Vladimir) Геннадьевич (Gennad'evich) Васильев (Vasiliev) ◽  
Татьяна (Tat'jana) Абдулхаиловна (Abdulhailovna) Кукушкина (Kukushkina)

The purpose of this paper was a comparative to study population variability of individual and group composition of biologically active phenol compounds in leaves of Lonicera caerulea subsp. pallasii (Ledeb.) Browic. in natural population of south taiga subzone of West-Siberian plain, Altai Mauntains and in conditions of introduction in the forest-steppe of the Novosibirsk Priobye. Chlorogenic acid and its isomers neochlorogenic acid and dicaffeoyl quinic acid, glycozides of luteolin, apigenin and quercetin, apigenin and luteolin in leaf extracts of L. caerulea subsp. pallasii was identified by HPLC-MS. The quantitative variations content of compounds (classes of biologically active polyphenols and their individual components) depending on vegetation conditions have been explored using HPLC. The major components of L. caerulea subsp. pallasii leaves are rutinoside quercetin, chlorogenic acid and dicaffeoyl quinic acid. The content and ratio of the major phenolic compounds and the composition of their minor components varied depending on the location and conditions of plant growth. The content of derivatives of hydroxycinnamic acids and flavonols increased significantly in response to changes in habitat conditions in natural populations and in the introduction into the forest-steppe zone. The flavones are the least variable compounds in plant leaves. The results can be useful in elaboration of practical recommendations for picking medicinal and food plants in different vegetation conditions


2021 ◽  
Vol 38 ◽  
pp. 00033
Author(s):  
Tatiana Fomina ◽  
Tatiana Kukushkina

The content of the major groups of biologically active substances in flowers of 7 Campanula species, cultivated in the forest-steppe of Western Siberia, were investigated for the first time. Freshly collected flowers were found to contain 0.06-0.15 % of catechins, 0.31-3.17 % of flavonols, 3.31-6.47 % of tannins, 3.10-15.57 % of pectic substances, and 0.66-16.4 mg% of carotenoids calculated per absolute dre weight of raw material. The amount of ascorbic acid was 36.9-114.0 mg% per wet weight. The data indicate that Campanula flowers are promising for the production of phenolic compounds, the development of food additives and fresh consumptions a seasoning.


2020 ◽  
Vol 5 (2) ◽  
pp. 3-7
Author(s):  
N. Trots ◽  
Aleksei Pakhomov

The aim of study is reduction of accumulation of heavy metals in agrocenosis of pulse crops due to the action of biologically active substances in the conditions of the middle Volga forest. An experiment study in order to reveal the effect of pre-sowing seed treatment with biologically active substances on the accumulation of heavy metals by soils and plants in agrocenoses of peas and soybeans was conducted in 2013-2015. The results of pre – sowing treatment of soybean seeds of the Samer 3 breed and peas of the Flagman 12 with the Rizotorfin and Rizotorfin in combination with analogues – Agrica and Humariz biologically active substance are presented. The analysis of heavy metals content in soil and plant samples was performed in the laboratory of the «Samara agrochemical ser-vice» Station by method of atomic adsorption spectroscopy. It is established that the introduction of biologically ac-tive substances into the soil containing heavy metals can reduce the concentration of their mobile forms and restrict access to grain. The content of mobile forms of elements in the soil under soybean growing areas, in comparison with the control, decreases: lead by 1.14 times when treated with Rhizotorphin+Humariz, cadmium – 1.18 times when treated with Rhizotorphin, copper – 1.2 times and cobalt – 2.0 times under the influence of the combination of Rhizotorphin and Agrica. According to the coefficients of biological absorption of pea and soy grains, the studied heavy metals are referred to the elements of biological capture of CАB<1. An effective inactivating effect on heavy metals is provided by pre-sowing treatment of pea and soy seeds with biologically active substances combined with Rhizotorphin+Agrica and Rhizotorphin+Humariz.


2018 ◽  
pp. 107-114
Author(s):  
Татьяна (Tat'yana) Ивановна (Ivanovna) Снакина (Snakina) ◽  
Татьяна (Tat'yana) Абдулхаиловна (Аbdulkhailovna) Кукушкина (Kukushkina)

Bog blueberry berries are a source material for food and pharmaceutical industries. However, industrial harvesting of bog blueberry is hardly carried out as plants grow in hard-to-access areas and safekeeping of berries is low during transportation to the processing plants.Special-purpose plantations of bog blueberry at the processing plants may solve the problem. To do this would require planting material noted for a high content of biologically active substances. Central Siberian Botanical Garden is a leading center of introduction and breeding of blueberry in Russia.In this connection, biochemical study of dry substance, ascorbic acid, sugars, acids, anthocyans, catechins, pectins and protopectins of berries of promising bog blueberry forms was conducted in the forest-steppe zone of West Siberia in 2011–2015.The results of research showed a wide range of variability of chemical characters due to individual peculiarities of bog blueberry forms and weather conditions in various years. Protopectins, catechins and pectins exhibited the greatest interannual distinctions.Bog blueberry samples with the greatest content of biologically active substances which can be recommended as planting material for creation of production plantations were revealed.


Author(s):  
T.V. Zubkova ◽  
◽  
V.L. Zakharov ◽  

The content of biologically active substances in fruit and vegetable products is an important parameter for de-termining the possibility of their use in the processing in-dustry. The research goal was to evaluate the yields and analyze the biochemical composition of different varieties of pumpkin grown in the forest-steppe of the Central Cher-nozem Region. It was found that of the studied pumpkin varieties, the maximum yields under these conditions were obtained from Seraya volzhskaya (12.7 t ha) and Zhem-chuzhina (9.3 t ha) varieties. The study of pulp chemical composition revealed that in terms of the content of caro-tene and P-active flavonols giving the brightest orange color to the pulp the variety Golosemyanka was the leading one. In terms of yield, this variety showed the medium indi-ces among the studied varieties -6.9 t ha. A fruit of this variety revealed the following content levels: carotene -20.24 mg %, ascorbic acid -20.24 mg %, and dry solids -7.4%. The Golosemyanka variety also revealed good or-ganoleptic indices. The pulp had a light orange color; the seeds lacked the usual hard shells. Therefore, the Golosemyanka variety may be recommended for use in the production of vegetable puree that will be characterized by high content of biologically active substances.


2021 ◽  
pp. 291-297
Author(s):  
Tat'yana Ivanovna Fomina ◽  
Tat'yana Abdulkhailovna Kukushkina

The results of comparative study of the content of biologically active substances in the flowers of 10 species of perennial onions Allium aflatunense B. Fedtsch., A. altaicum Pall., A. flavum L., A. microdictyon Prokh., A. nutans L., A. obliquum L., A. ramosum L., A. rosenbachianum Regel, A. schoenoprasum L., A. senescens L. var. glaucum Regel (A. senescens ssp. glaucum (Schrad.) N. Friesen) are presented. The freshly collected raw materials in the phase of mass flowering were analyzed. The dry matter content was determined by drying 1 g of raw material at 100–105 °C to a constant mass. The amount of phenolic compounds, pectin substances, total sugars, and carotenoids was determined spectrophotometrically using SF-56 (Russia) and SF "Agilent" 8453 (USA). For the first time, data on the quantitative content of the main groups of secondary metabolites in onion flowers were obtained. It has been established that in the forest-steppe of Western Siberia, flowers of Allium species contain: 10.7-22.5% dry substances, 6.6-26.2% total sugars, 3.3–18.6% pectin substances, 3.6–10.6% phenolic compounds, 3.0–23.4  mg% carotenoids (per mass of absolutely dry materials) and 35.4–157.8 mg% ascorbic acid (per mass of raw materials). The highest values were found for A. rosenbachianum, A. flavum, and A. aflatunense. The high variability of the accumulation of biologically active substances is due to the species and the influence of weather conditions during the growing season. The study showed the prospects for using onion flowers as a source of various bioactive compounds, including when added to food.


2011 ◽  
Vol 4 (2) ◽  
pp. 202-211 ◽  
Author(s):  
G. I. Vysochina ◽  
T. A. Kukushkina ◽  
O. V. Kotsupii ◽  
Yu. V. Zagurskaya ◽  
I. I. Bayandina

2020 ◽  
pp. 149-158 ◽  
Author(s):  
Yelena Petrovna Khramova ◽  
Yelena Vasil'yevna Bogolyubova ◽  
Tat'yana Ablulhailovna Kukushkina ◽  
Tat'yana Mihajlovna Shaldaeva ◽  
Galina Kimovna Zvereva

The article presents data on the content of biologically active compounds and the total activity of phenolic antioxidants in the leaves and inflorescences of the valuable forage plant Trifolium pannonicum Premier cultivar grown in different agroclimatic subareas of the Novosibirsk region – moderately warm, moist and moderately warm, not sufficiently moist. It is shown that, depending on the plant growth, biologically active substances are synthesized in differently: the content of flavonols, catechins, carotenoids and pectin substances is 1.1–1.6 times higher in plants from a more humid area. Tannins maximally (up to 17.43%) accumulate in the leaves of T. pannonicum, grown in an area with insufficient moisture. All samples of T. pannonicum variety Premier are characterized by high antioxidant activity of ethanol extracts from leaves and inflorescences (1.50–2.20 mg/g). The maximum of the total activity of antioxidants was found in the inflorescences of T. pannonicum from an area with insufficient moisture. Plant material T. pannonicum variety Premier is characterized by a fairly high content of biologically active substances.


2021 ◽  
pp. 145-154
Author(s):  
Irina Georgiyevna Boyarskikh

The aim of the study was to compare the variability of the composition, chemical identity and content of biologically active phenolic compounds in the fruits and leaves of the blue honeysuckle (Lonicera subsection Caeruleae) plants of different environmental and geographic provenances, sampled from the introduction plantation station in the forest steppe zone near the Ob River (Novosibirsk, Russia). In extracts from the leaves 25 individual compounds representing various polyphenolic classes were identified using HPLC-MS technique; seven of the compounds were identified as hydroxycinnamic acids, five compounds were flavonols, and eight compounds represented flavones. The maximal number of individual compounds was present in samples of the Altai subspecies of the tetraploid species L. caerulea subsp. altaica, whereas the minimal number was detected in samples of L. boczkarnikowae, a diploid species from the Russian Far East (Primorsky region). The lowest total polyphenolics content (6,260 mg/100 g of air-dry phytomass) was found in samples of L. caeruleae subsp. pallasii, whereas the content in samples from other blue honeysuckle subspecies ranged within 11.620-14.030 mg/100 g of air-dry phytomass. High content of flavones in extracts from leaves, always exceeding the flavonol content, was found to be a characteristic feature of L. caerulea subsp. altaica. Among L. сaerulea subspecies, L. сaerulea subsp. pallasii was shown to have the largest ratio of flavonols to flavones. L. boczkarnikowae also had high content of flavonols, significantly exceeding the flavones content in the plants organs. The main component of anthocyanins was cyanidin-3-glucoside, accounting for up to 91%. The fruits of L. caerulea subsp. altaica, L. сaerulea subsp. venulosa and L. boczkarnikowae had the highest anthocyanin content, ranging 2.950–3.200 mg/100 g air-dry phytomass, whereas the fruits of L. сaerulea subsp. pallasii had the lowest one (1,573 mg/100g). Extracts from the leaves were found to have significantly higher polyphenolics content as compared to the ones from the fruits; thus the leaves can be recommended as a prospective medicinal source.


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