scholarly journals VARIABILITY OF THE INDIVIDUAL-GROUP COMPOSITION OF POLYPHENOLS OF THE FRUITS AND LEAVES OF BLUE HONEYSUCKLE SAMPLES OF DIFFERENT ECOLOGICAL AND GEOGRAPHICAL ORIGIN IN THE OB FOREST-STEPPE

2021 ◽  
pp. 145-154
Author(s):  
Irina Georgiyevna Boyarskikh
Keyword(s):  
Russian Far East ◽  
Far East ◽  
Diploid Species ◽  
Steppe Zone ◽  
Biologically Active ◽  
Forest Steppe ◽  
Anthocyanin Content ◽  
The Individual ◽  
Main Component ◽  
Blue Honeysuckle

The aim of the study was to compare the variability of the composition, chemical identity and content of biologically active phenolic compounds in the fruits and leaves of the blue honeysuckle (Lonicera subsection Caeruleae) plants of different environmental and geographic provenances, sampled from the introduction plantation station in the forest steppe zone near the Ob River (Novosibirsk, Russia). In extracts from the leaves 25 individual compounds representing various polyphenolic classes were identified using HPLC-MS technique; seven of the compounds were identified as hydroxycinnamic acids, five compounds were flavonols, and eight compounds represented flavones. The maximal number of individual compounds was present in samples of the Altai subspecies of the tetraploid species L. caerulea subsp. altaica, whereas the minimal number was detected in samples of L. boczkarnikowae, a diploid species from the Russian Far East (Primorsky region). The lowest total polyphenolics content (6,260 mg/100 g of air-dry phytomass) was found in samples of L. caeruleae subsp. pallasii, whereas the content in samples from other blue honeysuckle subspecies ranged within 11.620-14.030 mg/100 g of air-dry phytomass. High content of flavones in extracts from leaves, always exceeding the flavonol content, was found to be a characteristic feature of L. caerulea subsp. altaica. Among L. сaerulea subspecies, L. сaerulea subsp. pallasii was shown to have the largest ratio of flavonols to flavones. L. boczkarnikowae also had high content of flavonols, significantly exceeding the flavones content in the plants organs. The main component of anthocyanins was cyanidin-3-glucoside, accounting for up to 91%. The fruits of L. caerulea subsp. altaica, L. сaerulea subsp. venulosa and L. boczkarnikowae had the highest anthocyanin content, ranging 2.950–3.200 mg/100 g air-dry phytomass, whereas the fruits of L. сaerulea subsp. pallasii had the lowest one (1,573 mg/100g). Extracts from the leaves were found to have significantly higher polyphenolics content as compared to the ones from the fruits; thus the leaves can be recommended as a prospective medicinal source.

PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY GLYCYRRHIZA PALLIDIFLORA HERB (GLYCYRRHIZA PALLIDIFLORA MAXIM.)

2021 ◽  
pp. 42-46
Author(s):  
Maltseva E.M. ◽  
Egorova I.N. ◽  
Pinchuk L.G.
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Russian Far East ◽  
Far East ◽  
Biologically Active ◽  
Perennial Herb ◽  
Total Phenolic ◽  
Plant Antioxidants ◽  
Phytochemical Studies ◽  
First Time

Pale-flowered licorice (Glycyrrhiza pallidiflora Maxim.) is the pea family (Fabaceae) perennial herb. It is a Russian Far East endemic. Pale-flowered licorice refers to the false (not sweet) licorice section (Pseudoglycyrrhiza Krug.), which do not accumulate glycyrrhizin derivatives. The G. pallidiflora successful introduction has been carried out over the past 5 years in the “Apothecary garden” territory, FRC UUH SB RAS, Kemerovo. Phytochemical studies screening biologically active compounds main classes of the plant aerial part confirmed the presence of a complex of phenolic compounds. The content of catechin derivatives and condensed-type tannins - proanthocyanidins (PAC) data in G. pallidiflora herb were obtained for the first time. In different years of cultivation, licorice herb accumulates phenolic compounds - up to 2.83 ± 0.22% in terms of gallic acid, flavonoids - up to 2.44 ± 0.03% in terms of rutin and PAC in terms of cyanidine chloride - up to 2.61 ± 0.11%. It was found that the maximum content of the phenolic compounds and PAC sum was observed in the herb for 4 years development. The greatest number of flavonoids accumulates in the herb harvested in the 3rd year of cultivation. A positive significant linear relation was found between antioxidant activity (AOA) and the total phenolic compounds and PAC content (r≥0.98). Considering that BAC with IC values ≤ 50 μg / ml in the DPPH assay refer to active antioxidant’s licorice herb can be classified as a plant with high antioxidant potential. It was found that the iron chelating activity of the G. pallidiflora herb is in direct dependence (r≥0.94) on the content of flavonoids. The obtained results demonstrate the importance of further study of this plant as a source of BAC, including plant antioxidants.

scholarly journals THE CONTENT OF BIOLOGICALLY ACTIVE POLYPHENOLS LONICERA CAERULEA SUBSP. PALLASII IN NATU-RAL CONDITIONS AND THE INTRODUCTION

2017 ◽  
pp. 89-96 ◽  
Author(s):  
Ирина (Irina) Георгиевна (Georgievna) Боярских (Boyarskikh) ◽  
Владимир (Vladimir) Геннадьевич (Gennad'evich) Васильев (Vasiliev) ◽  
Татьяна (Tat'jana) Абдулхаиловна (Abdulhailovna) Кукушкина (Kukushkina)
Keyword(s):  
Chlorogenic Acid ◽  
Natural Populations ◽  
Quinic Acid ◽  
Steppe Zone ◽  
Biologically Active ◽  
Food Plants ◽  
Forest Steppe ◽  
West Siberian Plain ◽  
Leaf Extracts ◽  
Lonicera Caerulea

The purpose of this paper was a comparative to study population variability of individual and group composition of biologically active phenol compounds in leaves of Lonicera caerulea subsp. pallasii (Ledeb.) Browic. in natural population of south taiga subzone of West-Siberian plain, Altai Mauntains and in conditions of introduction in the forest-steppe of the Novosibirsk Priobye. Chlorogenic acid and its isomers neochlorogenic acid and dicaffeoyl quinic acid, glycozides of luteolin, apigenin and quercetin, apigenin and luteolin in leaf extracts of L. caerulea subsp. pallasii was identified by HPLC-MS. The quantitative variations content of compounds (classes of biologically active polyphenols and their individual components) depending on vegetation conditions have been explored using HPLC. The major components of L. caerulea subsp. pallasii leaves are rutinoside quercetin, chlorogenic acid and dicaffeoyl quinic acid. The content and ratio of the major phenolic compounds and the composition of their minor components varied depending on the location and conditions of plant growth. The content of derivatives of hydroxycinnamic acids and flavonols increased significantly in response to changes in habitat conditions in natural populations and in the introduction into the forest-steppe zone. The flavones are the least variable compounds in plant leaves. The results can be useful in elaboration of practical recommendations for picking medicinal and food plants in different vegetation conditions

scholarly journals FLAVONOIDS OF THE EAST ASIAN SPECIES SERRATULA MANSHURICA KITAG.

2021 ◽  
pp. 167-173
Author(s):  
Aleksey Viktorovich Myagchilov ◽  
Larisa Ivanovna Sokolova ◽  
Petr Grigor'yevich Gorovoy
Keyword(s):  
Aerial Part ◽  
Russian Far East ◽  
Far East ◽  
Growing Season ◽  
Reversed Phase ◽  
Biologically Active ◽  
High Biological Activity ◽  
Flavonoid Aglycones ◽  
Promising Source ◽  
Maximum Content

One of the poorly studied and widespread plants in the Russian Far East is the Manchurian saw-wort (Serratula manshurica Kitag.). It is known that this plant is rich in phytoecdysteroids, which have high biological activity (anabolic, adaptogenic, cardiotropic, immunomodulatory effects). Two biologically active compounds of the flavonoid class were isolated from the aerial part of Serratula manshurica Kitag. Growing in the Primorsky Kray of the Far East of Russia by liquid extraction and recrystallization methods: quercetin-4'-O-β-D-glucuronopyranoside and apigenin-7-O-β-D-glucuronopyranoside. The structure of the isolated compounds was proved by UV-, NMR-spectroscopy and mass spectrometry with electrospray ionization. The quantitative content of flavonoids in the aerial part of the Serratula manshurica plant in different phenological phases of plant development (luteolin-4'-O-β-D-glucuronopyranoside, quercetin-4'-O-β-D-glucuronopyranoside, apigenin-7-O-β-D-glucuronopyranoside, 3-methylquercetin-4'-O-β-D-glucuronopyranoside, quercetin, apigenin, 3-methylquercetin, luteolin, isokaempferide) was determined by the method of reversed phase liquid chromatography (RP HPLC). It was shown that the maximum content of flavonoid aglycones in plants was observed during the beginning of the growing season and in the budding phase, and their glycosides - during the flowering phase. Thus, to obtain plant material with the maximum content of flavonoid aglycones (quercetin, 3-methylquercetin, luteolin) the collection of vegetative mass should be carried out during the beginning of the growing season, and their glycosides - during the flowering period. Serratula manshurica Kitag., which grows abundantly on the territory of Primorsky Krai, can be considered a constantly renewable and promising source for obtaining flavonoids.

scholarly journals Molecular and cytological evidences denied the immediate-hybrid hypothesis for Saxifraga yuparensis (sect. Bronchiales, Saxifragaceae) endemic to Mt. Yubari in Hokkaido, northern Japan

Phytotaxa ◽  
2018 ◽  
Vol 373 (1) ◽  
pp. 53 ◽  
Author(s):  
SAYA TAMURA ◽  
TOMOKO FUKUDA ◽  
ELENA A. PIMENOVA ◽  
EKATERINA A. PETRUNENKO ◽  
PAVEL V. KRESTOV ◽  
...  
Keyword(s):  
Russian Far East ◽  
Far East ◽  
Diploid Species ◽  
Coordinate Space ◽  
Herbarium Specimens ◽  
Snp Data ◽  
Genome Wide ◽  
Distinct Lineage ◽  
Bayesian Criteria ◽  
Northern Japan

SAYA TAMURA, TOMOKO FUKUDA, ELENA A. PIMENOVA, EKATERINA A. PETRUNENKO, PAVEL V. KRESTOV, SVETLANA N. BONDARCHUK, OLGA A. CHERNYAGINA, YOSHIHISA SUYAMA, YOSHIHIRO TSUNAMOTO, AYUMU MATSUO, HAYATO TSUBOI, HIDEKI TAKAHASHI, KEN SATO, YOKO NISHIKAWA, TAKASHI SHIMAMURA, HIROKO FUJITA & KOH NAKAMURA An alpine plant Saxifraga yuparensis is endemic to a scree consisting of greenschist of Mt. Yubari in Hokkaido, Japan and it has been proposed as an immediate hybrid derived from two species of the same section Bronchiales based on morphological intermediacy: namely S. nishidae, a diploid species endemic to a nearby cliff composed of greenschist and tetraploid S. rebunshirensis comparatively broadly distributed in Japan and Russian Far East. Saxifraga yuparensis is red-listed and it is crucial for conservation planning to clarify whether this is an immediate hybrid and lacks a unique gene pool. The immediate-hybrid hypothesis was tested by molecular and cytological data. In nuclear ribosomal and chloroplast DNA trees based on maximum parsimony and Bayesian criteria, S. yuparensis and S. rebunshirensis formed a clade with several other congeners while S. nishidae formed another distinct clade. Genome-wide SNP data clearly separated these three species in principal coordinate space, placing S. yuparensis not in-between of S. rebunshirensis and S. nishidae. Chromosome observation indicated that S. yuparensis is tetraploid, not triploid directly derived from diploid-tetraploid crossing. Additionally, observation of herbarium specimens revealed that leaf apex shape of S. yuparensis fell within the variation of S. rebunshirensis. These results indicate that S. yuparensis is not an immediate hybrid of S. rebunshirensis and S. nishidae but a distinct lineage and an extremely narrow endemic species, that deserves for intensive conservation.

Flora of the forest-steppe zone of West Siberia as a source of biologically active compounds

2011 ◽  
Vol 4 (2) ◽  
pp. 202-211 ◽  
Author(s):  
G. I. Vysochina ◽  
T. A. Kukushkina ◽  
O. V. Kotsupii ◽  
Yu. V. Zagurskaya ◽  
I. I. Bayandina
Keyword(s):  
West Siberia ◽  
Steppe Zone ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Forest Steppe ◽  
Active Compounds

scholarly journals Ecological and biological characteristics of Polygonatum multiflorum (L.) All. in Mordovia

2019 ◽  
Vol 8 (4) ◽  
pp. 43-48
Author(s):  
Marina Viktorovna Labutina ◽  
Tatiana Aleksandrovna Maskaeva ◽  
Nina Dmitrievna Chegodaeva
Keyword(s):  
Steppe Zone ◽  
Biological Characteristics ◽  
Wild Plant ◽  
Forest Steppe ◽  
Herbaceous Plant ◽  
Perennial Herbaceous Plant ◽  
General Terms ◽  
Real Risk ◽  
The Republic ◽  
The Individual

The paper discusses some of the ecological and biological characteristics of a perennial herbaceous plant - Polygonatum multiflorum seeds. Liliaceae. It is of interest due to the fact that the number of this species of the forest-steppe zone is reduced due to the increasing anthropogenic load. In addition, P. multiflorum is a valuable medicinal and ornamental plant. The studies were conducted in 2016-2017 on the territory of the Republic of Mordovia, in the surroundings of large and provincial cities. The study revealed the spatial and age structure of the four coenopopulations plants. The general terms of the vegetation P. multiflorum and the duration of the individual stages of plant development were determined. The level of potential and actual fruit formation is established. P. multiflorum plants were found scattered or in groups, but most often, the density was 3-4 individuals/m. The studied coenopopulations P. multiflorum are mostly normal, full-term. Renewal can be carried out both due to seeds and vegetatively. The latter is observed under favorable conditions for the P. multiflorum of existence. The studies have confirmed the view that the P. multiflorum is a fairly vulnerable forest species and there is a real risk of loss of the natural gene pool of this wild plant.

scholarly journals Medico-geoinformaton system for suicide prevention among adolescents

2021 ◽  
Vol 27 (4) ◽  
pp. 218-231
Author(s):  
Sergey Savin ◽  
Nicolay Kosykh
Keyword(s):  
Suicidal Behavior ◽  
Depressive Disorders ◽  
Russian Far East ◽  
Far East ◽  
Amur Region ◽  
Mathematical Methods ◽  
Information Models ◽  
Geoinformation Modeling ◽  
The Individual ◽  
The Russian Far East

The development of adequate measures to combat the spread of socially significant diseases requires the use of modern tools available to demographers and epidemiologists for entering, analyzing and visualizing data on the spread of population movement processes in space. A medical and demographic geoinformation system has been created to study the dynamics of suicidal behavior in the Amur region of the Russian Far East. The main factors involved in the psychopathogenesis of suicidal behavior are considered. The research was conducted using various ecological and mathematical methods to assess the quality of life of the human population in extreme the conditions of the Far Amur region of the East of Russia, taking into account various factors of interaction between the individual, society and the environment. The features of the spread of suicidal attempts in the Amur region are revealed. The set of the main causes and factors of low intensity characterizing the current suicidal situation in the Amur region of the Russian Far East is studied on the example of the Khabarovsk city. The medical-demographic and socio-psychological problems of suicidal behavior caused by depressive disorders are analyzed from the standpoint of the geoinformation modeling method. Original methodological approaches to the use of information models for medical-demographic and socio-economic studies of depressive spectrum disorders and the prevention of adolescents suicidal behavior are proposed.

scholarly journals To the History of the Discovery and Research into Hemorrhagic Fever with Renal Syndrome

2021 ◽  
pp. 33-38
Author(s):  
GG Kompanets ◽  
OV Iunikhina
Keyword(s):  
Russian Federation ◽  
Viral Infections ◽  
Russian Far East ◽  
Far East ◽  
Population Cycles ◽  
Hemorrhagic Fever ◽  
Biologically Active ◽  
Hantaan Virus ◽  
Relevant Today ◽  
The Russian Federation

Background: Hemorrhagic fever with renal syndrome (HFRS) is a viral, natural focal infection that is currently relevant for many countries of the world and ranks high among zoonotic viral infections in the Russian Federation. The purpose of our work was to present the main stages of the discovery and study of HFRS: from registration of the first clinical cases of the disease by military doctors in the east of our country to the current level of research. Materials and methods: We analyzed scientific literature devoted to the discovery of HFRS and further studies of this natural focal disease. Results: The discovery of many natural focal infections that are still relevant today in the Russian Federation coincided with the beginning of the rapid exploration and development of the Far East in the 1930s. Long-term studies of Soviet and foreign scientists helped systematize knowledge about viral etiology, clinical picture, pathophysiology, and pathomorphology of hemorrhagic nephrosonephritis and isolate the Hantaan virus, the etiological agent of HFRS (Lee HW, 1978). World famous Soviet virologists A.A. Smorodintsev and M.P. Chumakov, local scientists, doctors, and employees of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing made a significant contribution to the research into etiology, terminological consistency, epidemiology, and epizootology of HFRS. Conclusion: At present, the study of various aspects of hantavirus infections is one of the main tasks of the Research Institute of Epidemiology and Microbiology named after G.P. Somov. The research continues to establish the mechanisms of functioning of the natural foci of orthohantaviruses at different phases of the population cycles of their main carriers and in different landscape zones of the Russian Far East. A search for new species of orthohantaviruses, their natural reservoirs, and novel antiviral biologically active substances of natural and synthetic origin against orthohantaviruses is going on.

scholarly journals Tissue preparations as an eff ective means of correction of sexual cyclicalityof infertile cows in the conditions of forest-steppe zone in Zhytomyr region

2020 ◽  
pp. 6-11
Author(s):  
A. Revunets ◽  
G. Gryshchuk ◽  
Ya. Veremchuk
Keyword(s):  
Veterinary Medicine ◽  
Placental Tissue ◽  
Steppe Zone ◽  
Biologically Active ◽  
Sexual Cycle ◽  
Forest Steppe ◽  
Tissue Preparation ◽  
Animal Reproduction ◽  
Gynecological Diseases ◽  
Active Substances

Implementation of the latest technologies of animal reproduction causes changes in the life of their body and infl uences the manifestation of sexual cyclicity. As a result, one of the main tasks of specialists of veterinary medicine is the elimination of infertility of cows and heifers and improvement of methods of regulation of their reproductive functions. In recent years, in veterinary medicine for prophylactic and therapeutic purposes individually or in combination with other agents during pregnancy, in the postpartum period for gynecological diseases, to stimulate the functions of the sexual apparatus of animals, and their farming to improve productivity eff ectively used biogenic stimulators, that are made of tissues and organs of animals and plants – tissue preparations. But remain changes of the sexual apparatus of infertile cows are not studied in their use. Therefore, the purpose of the research was to study the eff ect of tissue preparations in a complex with ihglukovit for the correction of the sexual cycle of the infertile cows in the conditions of forest-steppe in Zhytomyr region. It has been found that the using tissue preparations make from the liver and placenta and ihglukovit for cows, time successful insemination was minimal and was 47.0±5.11 days. We also observed a positive eff ect on the usefulness of the fl ow of the sexual cycle when used in complex tissue preparations from the liver and placenta, as a consequence of infertility in the cow was of 17.00±1.14 days. As all experienced animals received ihglukovit, and the period of occurrence of complete sexual cycle and the eff ectiveness of insemination of cows was diff erent, then we assume that the changes in animals are due primarily to the infl uence of tissue preparations. We believe that the using of "placental" tissue preparation make from the uterus of pregnant cows with contents was the most eff ective, because it includes biologically active substances that are similar to the sexual apparatus of a cow. Analyzing the obtained results, we recommend to apply the tissue preparation liver, placenta and ihglukovit at a dose of 20 cm3 per animal, three times with an interval of 8-10 days to correct the sexual cycle of cattle and prevention of infertility. Key words: biogenic stimulant, tissue preparation, ihglukovit, reproduction, sexual cycle, infertility, cows.

scholarly journals CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES IN THE ABOVEGROUND PART OF SOME ONION SPE-CIES (ALLIUM L.)

2019 ◽  
pp. 177-184
Author(s):  
Tat'yana Ivanovna Fomina ◽  
Tat'yana Abdulkhailovna Kukushkina
Keyword(s):  
Ascorbic Acid ◽  
Raw Materials ◽  
Steppe Zone ◽  
Individual Variability ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Forest Steppe ◽  
Aboveground Part ◽  
Active Substances ◽  
Pectin Substances

The results of comparative study of the content of biologically active substances in the aboveground part of 11 species of perennial onions Allium aflatunense B. Fedtsch., A. altaicum Pall., A. flavum L., A. microdictyon Prokh., A. nutans L., A obliquum L., A. ramosum L., A. rosenbachianum Regel, A. schoenoprasum L., A. senescens L. var. glaucum Regel (A. senescens ssp. glaucum (Schrad.) N. Friesen), A. strictum Schrad. are presented. The freshly collected raw materials in the phase of consumer ripeness were analyzed and all indicators, except for the amount of ascorbic acid, were calculated on the mass of absolutely dry raw materials. It was established in onions a high content of dry substances (up to 25.1%), flavonols (up to 3.4%), tannins (up to 14.6%), pectin substances (up to 25.8%), sugars (up to 34.8%), ascorbic acid (up to 222.5 mg% ), carotenoids (up to 131.9 mg%); the content of catechins was insignificant, at the level of 0.04–0.15%, only in A. rosenbachianum – up to 0.56%. The contents of catechins, tannins, pectin substances in onions were determined for the first time. A high interspecific and individual variability of accumulation of biologically active substances was revealed. A. rosenbachianum, A. aflatunense, A. flavum, A. microdictyon have the greatest contents and relatively low indicators are in A. senescens var. glaucum. The species are promising for cultivation in the forest-steppe zone of Western Siberia as early vitamin-bearing plants and as a source of various biologically active compounds.

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