scholarly journals Liberica Coffee (Coffea liberica L.) from Three Different Regions: In Vitro Antioxidant Activities

2021 ◽  
Vol 11 (5) ◽  
pp. 13031-13041
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Unstable Molecules ◽  
Coffea Liberica

Free radicals are unstable molecules with unpaired electrons in their outer orbitals. An antioxidant is a compound that can be scavenged free radicals. Coffee is one of the natural antioxidants. This research aimed to study the antioxidant activity of medium roasted beans of liberica coffee (Coffea liberica) from three different regions by DPPH and CUPRAC methods. To determine total phenolic content (TPC) and total flavonoid content (TFC), analyze the correlation between TPC and TFC with AAI DPPH and CUPRAC and the correlation between two methods in sample extracts. The sample was extracted by reflux using n-hexane, ethyl acetate, and ethanol. AAI DPPH in the range of 0.397- 18.536, while CUPRAC 0.532-4.674. The highest TPC in ethanol extract of liberica coffee from Aceh (22.585 ± 1.610 g GAE/100 g) and the highest TFC in ethyl acetate extract of liberica coffee from Aceh (4.927 ± 0.355 g QE/100 g). TPC of all samples had a positive and significant correlation with AAI DPPH and CUPRAC. AAI DPPH and CUPRAC value gave a significant and positive correlation. TPC of liberica coffee from three different regions contributed to antioxidant activity by DPPH dan CUPRAC methods. The two methods showed linear results in antioxidant activities of liberica coffee from three regions.

scholarly journals Antioxidant activities of Dichrostachys cinerea (L.) Wight et Arn (Leguminosae): Correlations between the Polyphenol Level and the Antioxidant Activity

2020 ◽  
pp. 1-11
Author(s):  
Reine Raïssa Rolande Aworet Samseny ◽  
Line-Edwige Mengome ◽  
Sophie Aboughe Angone
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Antiradical Activity ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Dpph Radical ◽  
Dichrostachys Cinerea ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Aims: Free radicals are involved in many diseases. Antioxidants help control them. The overproduction of free radicals or reduction of natural antioxidants promotes the destruction of cells in our body, for example in neurodegenerative diseases. The aim of our study is to evaluate the correlation between the level of polyphenol and antioxidant activity of Dichrostachys cinerea barks he work was carried out at the phytochemistry department of the Institute of Pharmacopoeia and Traditional Medicine (Gabon). Methodology: Different extracts of Dichrostachys cinerea barks were obtained with polarity solvent (dichloromethane, ethyl acetate, butanol, methanol and water).  The antioxidant activity is evaluating using respectively FRAP method (Ferric Reducing Antioxidant Power), antiradical activity by the method of inhibition of DPPH radical (2.2-diphenyl-1-picrylhydrazyl), cations ABTS radical (2.2'-azinobis-[3-acid-6-sulfonic ethylenzothiazoline]). Results: Ethyl acetate and dichloromethane extract have the higher value for polyphenolic compounds, respectively 52,27±0,66 mg EAG / g and 49,72±0,55 mg EAG / g. Aqueous extract have the lower value 20,67±1,05 mg EAG / g. The different fractions of Dichrostachys cinerea have antioxidant effect, and this effect is in correlation with its constituents: polyphenols, tannins and flavonoids. Results were compared to standard antioxidants such as ascorbic acid and quercetin. Conclusion: This study confirms higher is the level of polyphenols greater is the antioxidant power.

scholarly journals QUANTIFICATION OF PHYTOCHEMICAL CONSTITUENTS AND IN VITRO ANTIOXIDANT ACTIVITY IN THE LEAVES OFCITRUS MEDICA

2017 ◽  
pp. 119-123
Author(s):  
Mayavatis Patil
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Gallic Acid ◽  
Ethanol Extract ◽  
Maximum Level ◽  
Maximum Activity ◽  
Total Phenolic ◽  
Alkaloid Contents ◽  
In Vitro Antioxidant Activity

Objective: In the present investigation, phytochemical assay and in vitro antioxidant activity of ethanol (70%), methanol, ethyl acetate and hexane extract of Citrus medica leaves were carried out.Methods: The quantification of total phenolic and alkaloid contents were estimated by taking gallic acid and atropine as standard. In–vitro antioxidant activity of extracts was evaluated by using different free radicals (DPPH, superoxide and free radicals).Results: Ethanol extract of leaves have more phenolic and alkaloid contents than other extracts. The selected plant extract was produced concentration dependent percentage inhibition of different free radicals and produced maximum activity at a concentration of 1000µgm/ml, and there after percentage inhibition was raised gradually to its maximum level with higher concentrations. HPLC analysis revealed the presence of Gallic acid, Catechein, Rutin, Chlorgenic acid, Queracetine and some unknown components which need to be e identified.Conclusion: Among the four extracts, ethanol extract of C. medica showed good antioxidant activity. 

scholarly journals Antioxidant activities of methanolic extract and its fractions of Baccaurea racemosa and Macaranga subpeltata leaves

Food Research ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 127-134 ◽  
Author(s):  
H. Widodo ◽  
Sismindari ◽  
W. Asmara ◽  
Abdul Rohman
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Food Supplement ◽  
Positive Control ◽  
The Body ◽  
Total Phenolic ◽  
Vitro Assay ◽  
Water Fraction

Oxidative stress, the excessive presence of reactive oxygen species (ROS), is suggested as a basal cause of aging as well as various degenerative and chronic diseases in human. Antioxidants are believed to play a very vital role in the body defense system against ROS. Plant-based antioxidants with their prominence have gained tremendous worldwide interest nowadays. Baccaurea racemosa and Macaraanga subpeltata are among ethnomedical used plants for liver diseases medication which have potential source as natural antioxidants. The aim of the study was to evaluate the antioxidant activities of the methanolic crude extract (CE) and their fractions of the plant’s leaves. Maceration was performed to obtain CE, which then subjected to fractionation using n-hexane, dichloromethane, chloroform, ethyl acetate, and ethanol to obtain fractions of hexane fraction (HF), dichloromethane (DF), chloroform (CF), ethyl acetate (EAF), and ethanol fractions (EF), respectively. The CE and all fractions included water fraction (WF) and residue (R) were examined for its total phenolic contents, total flavonoid contents, and antioxidant activities using various in vitro assay. In general, EAF demonstrated as the best solvent for the extracting phenolic compounds with higher antioxidant activity. The CE and its fractions of M. subpeltata contained higher of TPC and TFC, also demonstrated higher antioxidant capacity, than that B. racemosa. The phenolics compounds were responsible for the antiradical properties. The EAF of M. subpeltata was scavenging those radicals better than that of L-(+)ascorbic acid as a positive control. The high antioxidant activities and phenolics contents make both the plant extracts to be developed as a food supplement.

scholarly journals In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts

2020 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mounira Merghem ◽  
Saliha Dahamna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Ruta Montana ◽  
Β Carotene

The aim of this study is to evaluate in vitro antioxidant activities of Ruta montana L.  extracts. This activity was evaluated by three methods : DPPH (2, 2'-diphenyl- 1- picrylhydrazy), bleaching of β-carotene and chelation of ferrous iron. Results showed that ethyl acetate extract (EAE) represents the highest amount of total polyphenols, tannins and flavonoids with 257,1 ± 0,703µg gallic acid equivalent/mg of extract,  251 ± 1.41 µg tannic acid equivalent /mg of extract,117,4 ± 3,451 µg quercetin equivalents/mg of extract, 139,5 ± 4,107 µg rutin equivalents/mg of extract, respectively. In the DPPH assay, ethyl acetate extract showed the higher scavenging capacity (IC50 = 0.044 ± 0.001 mg/ml) followed by methanol, aqueous and chloroform extract. Whereas, AqE showed the best chelating effect and the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene. Keywords: Ruta montana L; polyphenols; antioxidant activity; free radical scavenging.

scholarly journals Crystal Guava (Psidium guajava L. “Crystal”): Evaluation of In Vitro Antioxidant Capacities and Phytochemical Content

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Rika Hartati ◽  
Hashifah I. Nadifan ◽  
Irda Fidrianny
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Activity Index ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Positive Correlation ◽  
Phytochemical Content ◽  
Fruit Extracts

Free radicals can cause many diseases, such as cancer. Antioxidant is a compound that could scavenge free radicals. One of the natural antioxidants is guava. The goals of this research were to investigate the antioxidant activity of leaves and fruit of crystal guava by determining the value of the Antioxidant Activity Index (AAI) using DPPH, CUPRAC, and FRAP; evaluate the total phenolic content (TPC) and total flavonoid content (TFC); analyse the correlation between the TPC and TFC with AAI DPPH, CUPRAC, and FRAP, and analyse the correlation between the 3 methods. Extraction was performed by the reflux method using n-hexane, ethyl acetate, and ethanol. Determination of AAI DPPH, CUPRAC, FRAP, the TPC, and the TFC was performed by UV-visible spectrophotometry. The correlation of the TPC and TFC with AAI DPPH, CUPRAC, and FRAP and, also, the correlation of the 3 methods were investigated by Pearson’s method. The antioxidant activity of leaves and fruit extracts of crystal guava showed AAI DPPH in the range of 0.33–56.46, CUPRAC 0.20–7.31, and FRAP 1.65–59.89. The highest TPC was given by ethanol leaf extracts (49.55 ± 1.45 g GAE/100 g), while the highest TFC was for n-hexane leaf extracts (9.68 ± 0.210 g QE/100 g). The TPC of leaves extract had a significantly positive correlation with AAI DPPH, CUPRAC, and FRAP. AAI DPPH, AAI CUPRAC, and AAI FRAP of leaves and fruit extract of crystal guava showed a significantly positive correlation. In general, leaves extract had strong antioxidant activity by the three methods. For the highest antioxidant activity, ethanol was the best solvent for extraction leaves and ethyl acetate for extraction fruit of crystal guava. The TPC in leaves extract contributed to the antioxidant activity by DPPH, CUPRAC, and FRAP methods. The Antioxidant activity of leaves and fruit extracts of crystal guava was linear by the three methods.

Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Frap Assay ◽  
Phytochemical Composition ◽  
Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

scholarly journals Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
E. S. Prasedya ◽  
A. Frediansyah ◽  
N. W. R. Martyasari ◽  
B. K. Ilhami ◽  
A. S. Abidin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Particle Sizes ◽  
Epicatechin Gallate ◽  
Particle Size Reduction ◽  
Phytochemical Composition

AbstractSample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

scholarly journals Establishing Relationship between Vitamins, Total Phenolic and Total Flavonoid Content and Antioxidant Activities in Various Honey Types

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4399
Author(s):  
Norhasnida Zawawi ◽  
Pei Juin Chong ◽  
Nurul Nadhirah Mohd Tom ◽  
Nurkhairina Solehah Saiful Anuar ◽  
Salma Malihah Mohammad ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Extrinsic Factors ◽  
Biochemical Compounds ◽  
Phenolic And Flavonoid

Honey is a well-known natural sweetener and is rich in natural antioxidants that prevent the occurrence of oxidative stress, which is responsible for many human diseases. Some of the biochemical compounds in honey that contribute to this property are vitamins and phenolic compounds such as phenolic acids and flavonoids. However, the extent to which these molecules contribute towards the antioxidant capacity in vitro is inconsistently reported, especially with the different analytical methods used, as well as other extrinsic factors that influence these molecules’ availability. Therefore, by reviewing recently published works correlating the vitamin, total phenolic, and flavonoid content in honey with its antioxidant activities in vitro, this paper will establish a relationship between these parameters. Based on the literature, vitamins do not contribute to honey’s antioxidant capacity; however, the content of phenolic acids and flavonoids has an impact on honey’s antioxidant activity.

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