scholarly journals Heavy metal contamination and antibiotic residues in poultry feed and meat in Bangladesh

2021 ◽  
Vol 5 (2) ◽  
pp. 71-78
Author(s):  
Md Mahbubul Alam ◽  
Dwijendra Lal Mallick ◽  
Md Murshidul Ahsan ◽  
AHM Taslima Akhter ◽  
- Eftesum ◽  
...  
Keyword(s):  
Poultry Feed ◽  
Enzyme Linked Immunosorbent Assay ◽  
Meat Sample ◽  
Poultry Production ◽  
Antibiotic Residues ◽  
The Mean ◽  
Meat Samples ◽  
Local Farm ◽  
Mean Concentration ◽  
Feed Samples

Presence of harmful contaminants and residues in poultry feed and meat have serious public health consequence. This study was carried out to identify and quantify antibiotic residues, heavy metals and toxins in poultry feed and meat in the two selected poultry production belts of Bangladesh. A total of 94 broiler feed samples and 60 broiler meat samples were collected and tested by Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC) and Enzyme-linked Immunosorbent Assay (ELISA) for identification and quantification of the parameters. Antibiotic residues were detected in 18.89% of the feed samples, whereas, there were no toxin (Aflatoxin) positive samples. Among the antibiotic positive samples, Oxytetracycline (OTC) was found predominant and detected in 12.22% cases. The mean concentrations of Cadmium (Cd), Lead (Pb), Chromium (Cr) were found as 0.04 mg/kg, 1.28 mg/kg and 2.55 mg/kg respectively in feed samples. In the case of meat samples, the mean concentration of OTC, Ciprofloxacin (CIP), and Tetracycline (TCL) residues were found 8.67 ppb, 7.18 ppb and 0.81 ppb accordingly. The highest mean concentration of Oxytetracycline (OTC) (10.15 ppb) was found in samples collected from local poultry sellers, whereas, the highest mean concentration of Tetracycline (TCL) (1.35 ppb) and Ciprofloxacin (CIP) (10.62 ppb) were observed in the samples obtained from local farm. The highest percentage of TCL and CIP (64% and 48% respectively) were found in samples collected from local farm. Chlortetracycline (CTC) was found predominant (70%) in samples collected from Contract farms. On the other hand, out of 60 meat samples, Cd and Cr were detected in only one meat sample with concentration of 56.41 mg/kg and 14.44 mg/kg respectively. Lead was not detected in any of the meat samples. Asian Australas. J. Food Saf. Secur. 2021, 5 (2), 71-78

scholarly journals A Limited Survey of Aflatoxins and Zearalenone in Feed and Feed Ingredients from Pakistan

2016 ◽  
Vol 79 (10) ◽  
pp. 1798-1801 ◽  
Author(s):  
SHAHZAD ZAFAR IQBAL ◽  
MUHAMMAD RAFIQUE ASI ◽  
SONIA NISAR ◽  
KHALID MAHMOOD ZIA ◽  
SELAMAT JINAP ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Poultry Feed ◽  
Fluorescence Detector ◽  
Limit Of Quantification ◽  
Feed Ingredients ◽  
Cotton Seed Meal ◽  
The Mean ◽  
Mean Concentration ◽  
Feed Samples

ABSTRACT This work presents current information on the presence of aflatoxins (AFs) and zearalenone (ZEN) in feed and feed ingredients from Punjab, Pakistan. The 105 samples tested were concentrated feed, i.e., cotton seed meal (18 samples) and soybean meal (14), and feed ingredients, i.e., crushed corn (17), crushed wheat (15), barley (17). and poultry feed (24). Samples were analyzed using high-performance liquid chromatography equipped with a fluorescence detector. Analysis revealed that 69 of 105 samples were contaminated with AFs, and the highest mean concentrations of AFB1 (6.20 μg/kg) and total AFs (9.30 μg/kg) were found in poultry feed samples. The mean total AF concentrations ranged from the limit of quantification to 165.5 μg/kg. However, 75 of the 105 samples were positive for ZEN. The highest mean concentration (19.45 μg/kg) was found in poultry feed samples. The mean ZEN concentrations were 0.15 to 145.30 μg/kg. The prevalence of AFs and ZEN was high in feed and feed ingredients and needs urgent attention.

scholarly journals Status of Antibiotic Residues in Poultry Meat of Nepal

2018 ◽  
Vol 35 ◽  
pp. 55-62 ◽  
Author(s):  
M. Prajapati ◽  
E. Ranjit ◽  
R. Shrestha ◽  
S.P. Shrestha ◽  
S.K. Adhikari ◽  
...  
Keyword(s):  
Health Sector ◽  
Poultry Meat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Poultry Production ◽  
Antibiotic Residues ◽  
Animal Products ◽  
Consumer Safety ◽  
Livestock Products ◽  
Antibiotics Residues ◽  
Meat Samples

Antibiotic residues in livestock products are the burning issue in animal production as well as public health sector. Imprudent use of antibiotics in poultry production increases the risk of antibiotic resistance thus increasing risk in both animals and humans. To determine the trend of antibiotic residues presence in poultry meat, a study was conducted from June 2016 to May 2017 in Kathmandu, Kaski, and Chitwan districts of Nepal. A total of 92 breast samples of broilers ( Kathmandu-30, Kaski-40 and Chitwan-22) were collected randomly from different selling outlets of the study areas and tested for residues of commonly used antibiotic viz. Enrofloxacin (EX), Ciprofloxacin (CIP), Streptomycin (STR) and Chloramphenicol (CHL) using the Enzyme Linked Immunosorbent Assay (ELISA) technique. Out of 92 samples, 57 (62%) samples were found positive for antibiotics residue of which 38% samples were positive for STR residue, 15.2% for CIP and 8.7% for EX. However, none of the samples showed positivity for CHL residue. The level of STR was determined to be higher in all districts in comparison to other antibiotics. The meat samples from Kaski were found to contain higher level of STR (56.1%) while highest percentage of CIP (16.1%) was detected in Kathmandu. Also, presence of EX was found highest in Kathmandu compared to other districts. The study concluded that antibiotics residues have been abundantly found in poultry meat which can have serious effect in human health. Hence, to mitigate this, routine antibiotics residues monitoring and surveillance programmes in food animal products should be conducted to ensure the consumer safety.

scholarly journals Intact transferrin receptors in human plasma and their relation to erythropoiesis

Blood ◽  
1990 ◽  
Vol 75 (1) ◽  
pp. 102-107 ◽  
Author(s):  
HA Huebers ◽  
Y Beguin ◽  
P Pootrakul ◽  
D Einspahr ◽  
CA Finch
Keyword(s):  
Human Plasma ◽  
Polyclonal Antibodies ◽  
Close Correlation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Transferrin Receptors ◽  
Erythroid Hyperplasia ◽  
The Mean ◽  
Tissue Receptors ◽  
Mean Concentration ◽  
Normal Adults

Abstract Intact transferrin receptor molecules complexed with transferrin were found in human plasma. The concentration of receptors was determined by an enzyme-linked immunosorbent assay that uses polyclonal antibodies. The mean concentration of 8,279 micrograms/L in 56 normal adults appears to be unrelated to age or sex. Additional receptor measurements were performed on plasmas from 260 subjects with erythropoietic disorders. Decreased concentration of plasma receptors was found in patients with erythroid hypoplasia and increased numbers in those with erythroid hyperplasia. Ferrokinetic measurements of erythropoiesis were compared with numbers of receptors in 148 subjects, and a close correlation was found (r = .86). Both sets of values, measured in different conditions and expressed in relation to normal, were consistent with expected values. Receptor values were unproportionally increased only in conditions of iron deficiency. It is concluded that plasma receptors have a constant relationship to tissue receptors, and their number in most instances reflects the rate of erythropoiesis.

scholarly journals An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis

2012 ◽  
Vol 1 (2) ◽  
pp. 93-97
Author(s):  
Vinay Vadvadgi ◽  
Neeta Padmawar
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Gingival Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Group Iii ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
Crevicular Fluid ◽  
Mean Concentration

ABSTRACT Background and objective Plasma leptin is associated in patients with inflammatory diseases. A high concentration of leptin is associated with healthy gingival tissue. The purpose of this study was to assess the concentration of human leptin in gingival crevicular fluid (GCF) and serum within healthy and diseased gingiva, further to explore the possibility of using the levels of leptin in GCF and serum as a biochemical marker of periodontal disease progression. Materials and methods Ninety subjects were selected with age (30-39 years) and sex (15 males and 15 females) matched, to eliminate age and sex as confounders. The subjects were divided into three groups consisting of 30 subjects in each group based on the clinical and radiological parameters; healthy (group I), gingivitis (group II), periodontitis (group III), from whom the GCF samples were collected with Periopaper GCF collection strips (Proflow, Amityville, NY, USA) for 30 seconds and blood samples with 20-gauge needle syringe respectively. Leptin concentration was determined from individual GCF and serum samples by enzyme-linked immunosorbent assay (ELISA). Results The highest mean leptin concentration in GCF was observed in group I (2,664.30 pg/ml ± 324.73) and least mean leptin concentration was obtained in group III (1,309.43 pg/ml ± 202.45). The mean concentration of group II (1,639.43 pg/ml ± 344.46) was intermediate between the highest and lowest values. In contrast, the highest mean leptin concentration in serum was obtained for group III (12,086.57 pg/ml ± 1,698.23) and least mean leptin concentration was obtained for group I (8,715.09 pg/ml ± 1,649.19). The mean concentration of the group II (10,694.01 pg/ml ± 1,777.72) were intermediate between the highest and lowest values. Conclusion The results indicated a statistically significant decrease in the GCF leptin concentration and increase in serum leptin concentration as the periodontal disease progressed. How to cite this article Vadvadgi VH, Saini R, Padmawar N. An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis. Int J Experiment Dent Sci 2012;1(2):93-97.

scholarly journals Microelements and heavy metals in raw cow milk from various regions in Serbia

2013 ◽  
Vol 67 (5-6) ◽  
pp. 317-328 ◽  
Author(s):  
Milica Zivkov-Balos ◽  
Sandra Jaksic ◽  
Zeljko Mihaljev ◽  
Zeljko Cupic ◽  
Igor Stojanov ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Rural Areas ◽  
Animal Feed ◽  
Raw Milk ◽  
Industrial Area ◽  
Atomic Absorption Spectrophotometry ◽  
Cow Milk ◽  
The Mean ◽  
Mean Concentration ◽  
Feed Samples

In this study, the investigation results of raw milk and animal feed samples from farms located around industrial areas and from rural areas in Serbia are presented. Various microelements and heavy metals (Zn, Cu, Fe, Pb and Cd) were determined by atomic absorption spectrophotometry (AAS). Mean concentration of Zn, Cu, Fe and Cd in raw milk were 5.94, 0.239, 2.034 and below 0.005 mg/kg, respectively. The mean lead concentration found in milk from a farm located near industrial area was 0.242?0.166 mg/kg (mean Pb concentrations for other four investigated farms were below 0.05 mg/kg). The obtained results indicate that particular attention should be paid to control of Pb residues in food, water and environment.

Correlation between some quality parameters and bleeding status of poultry meat

2015 ◽  
Vol 34 (4) ◽  
Author(s):  
Tanbir Ahmad ◽  
Yogesh Kumar ◽  
Nilesh Gaikwad ◽  
Deepak Raj Rai
Keyword(s):  
Malachite Green ◽  
Traditional Method ◽  
Quality Parameters ◽  
Water Holding Capacity ◽  
Poultry Meat ◽  
Meat Sample ◽  
Retail Meat ◽  
The Mean ◽  
Meat Samples ◽  
Water Holding

A study was undertaken to investigate the effect of two different slaughter methods <italic>i.e</italic> traditional method (unilateral neck cut) and decapitation on the colour, pH, total heme pigments (THP) and water holding capacity (WHC) of the poultry meat. Fresh drumsticks (30 from conventionally slaughtered and 30 from slaughtered by decapitation method) of broiler birds (6 weeks of age; 2 hrs postmortem) were procured from retail meat shops located in different regions of the Ludhiana, Punjab, India. <italic>L*</italic> value (53.55) of conventionally slaughtered meat samples was significantly (P<0.01) higher than the decapitation method (47.97) but <italic>a*</italic> and <italic>b*</italic> values for conventionally slaughtered samples (7.86 and 12.62, respectively) were significantly (P<0.01) lower than the decapitated samples (11.42 and 15.90, respectively). These effects were due to the fact that the decapitated birds lost the least amount of blood compared to the conventionally slaughtered birds. pH of the conventionally slaughtered meat samples (6.02) were significantly (P<0.01) lower than the decapitated meat samples (6.32). The mean total heme pigments were significantly (P<0.01) higher in decapitated meat samples (2.20 mg/gm of meat sample) than the conventionally slaughtered meat samples (1.84 mg/gm of meat sample). Similarly WHC was significantly (P<0.01) higher of decapitated meat samples (52.91%) than the conventionally slaughtered meat samples (43.18%). Malachite Green test in 86.36% conventionally slaughtered meat samples showed perfect bleeding and 100% decapitated meat samples showed imperfect bleeding.

scholarly journals Assessment of Antibiotic Residues in the Marketed Meat of Kailali and Kavre of Nepal

2017 ◽  
Vol 5 (3) ◽  
pp. 386-389 ◽  
Author(s):  
Rabin Raut ◽  
Ram Kumar Mandal ◽  
Krishna Kaphle ◽  
Dinesh Pant ◽  
Suresh Nepali ◽  
...  
Keyword(s):  
Rapid Test ◽  
Cross Sectional Study ◽  
Meat Sample ◽  
Antibiotic Residues ◽  
Maximum Residue Limit ◽  
Cross Sectional ◽  
Test Kit ◽  
Sterile Plastic ◽  
Meat Samples ◽  
High Level

This is a cross-sectional study to detect presence of antibiotic residues in meat sample of Kailali and Kavre districts. Each sample out of 55 meat samples (muscles and liver) of poultry-41, goat-12, buffalo-9 and pig-4 from Kailali and Kavre was tested for different groups of antibiotic residues separately. The antibiotics tested were Penicillin, Tetracycline, Aminoglycosides, Macrolides and Sulfonamides. Samples were preserved at -84˚C in the deep freezer after collecting in sterile plastic pouch. Rapid test kit (78.9% sensitivity and 96.7% specificity) of G9 Co. Ltd., Thailand was used to detect residues in the samples. The protocol of kit was followed for the test. The overall prevalence of antibiotic residues in meat was around 22%. Meat samples exceeding the Maximum Residue Limit (MRL) were 6. Liver was found with higher number of residues than muscles. The organ and location wise prevalence of residue was found statistically significant at p<0.05. The samples contained alarmingly high level of antibiotic residues which is because of neglecting the withdrawal period. The prescription of antibiotic is not prudent. Int. J. Appl. Sci. Biotechnol. Vol 5(3): 386-389

Burden of Aflatoxin in Poultry Feeds in Selected Chicken Rearing Villages of Bishoftu-ethiopia

2020 ◽  
Author(s):  
Yoseph Cherinet Megerssa ◽  
Tadesse Sisay Kassaw ◽  
Fanos Tadesse Woldemariyam
Keyword(s):  
Human Health ◽  
Aflatoxin Contamination ◽  
Poultry Feed ◽  
Preventive Strategies ◽  
Poultry Industry ◽  
Sectional Study ◽  
Cattle Feed ◽  
Poultry Feeds ◽  
The Mean ◽  
Feed Samples

Abstract Background: Aflatoxins are major contaminants of feed used in poultry industry that negatively affect animal and human health. In Ethiopia, previous studies on aflatoxins mainly considered cattle feed and milk, but scarce information exists for poultry feeds.Method: The aim of this study was to determine the burden of aflatoxin in poultry feed in bishoftu.Cross sectional study was conducted from December, 2018 to May, 2019and 33 compound poultry feed samples were randomly collected from chicken rearing villages of Bishoftuand analyzed for G2, G1, B2 , B1 and total aflatoxins using HPLC.Results: The result indicated thatfrom a total of 33 samples 31(94%) samples were contaminated with aflatoxin. The mean level of aflatoxin G2, G1, B2, B1 and total aflatoxinswere 18.00 µg/g, 88.5499 µg/g, 13.50µg/g, 70.11µg/g and 190.18µg/g respectively. This study curtained the level of aflatoxinin 25 (72.75%) samples for AFT and 22 (66.67%) samples for AFB1 were above the limit of FDA regulatory levels of 20µg/g for poultry feed. Conclusion: The study showed the high contamination of aflatoxins in poultry feed. The study warrants the need for preventive strategies of aflatoxin contamination including implementation of regulatory legislation in poultry feeds in Bishoftu.

scholarly journals Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect Fusarium verticillioides in Poultry Feed Samples

Toxins ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 48 ◽  
Author(s):  
Aline Omori ◽  
Elisabete Ono ◽  
Melissa Hirozawa ◽  
Igor de Souza Suguiura ◽  
Elisa Hirooka ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Health Hazard ◽  
Fusarium Verticillioides ◽  
Cross Reactivity ◽  
Poultry Feed ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Feed Samples

Fumonisins are a group of toxic secondary metabolites that are produced by Fusarium verticillioides which are associated with poultry health hazard and great economic losses. The objective of the present study was to develop an immunological method to detect F. verticillioides in poultry feed samples. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a polyclonal antibody against 67 kDa protein of the F. verticillioides 97K exoantigen was developed to detect this fungus. Antibody anti-67 kDa protein showed cross-reactivity against F. graminearum (2–7%) and F. sporotrichioides (10%), but no or low cross-reactivity against Aspergillus sp. and Penicillium sp. exoantigens. The detection limit for the 67 kDa protein of F. verticillioides was 29 ng/mL. Eighty-one poultry feed samples were analyzed for Fusarium sp. count, 67 kDa protein of F. verticillioides and fumonisin concentrations. Eighty of the 81 feed samples (98.6%) showed Fusarium sp. contamination (mean 6.2 x 104 CFU/g). Mean 67 kDa protein and fumonisin concentration in the poultry feed samples was 21.0 µg/g and 1.02 µg/g, respectively. The concentration of 67 kDa protein, as determined by ic-ELISA correlated positively (p < 0.05) with fumonisin levels (r = 0.76). These results suggest that this ic-ELISA has potential to detect F. verticillioides and predict fumonisin contamination in poultry feed samples.

Occurrence of aflatoxin M1 in traditional cheese consumed in Esfahan, Iran

2009 ◽  
Vol 2 (1) ◽  
pp. 91-94 ◽  
Author(s):  
E. Rahimi ◽  
G. Karim ◽  
A. Shakerian
Keyword(s):  
Statistical Analysis ◽  
Human Health ◽  
Tolerance Limit ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Traditional Cheese ◽  
Maximum Tolerance ◽  
The Mean ◽  
Autumn And Winter ◽  
Mean Concentration

Aflatoxin M1 (AFM1) was determined by enzyme linked immunosorbent assay in 88 samples of traditional cheese consumed in Esfahan city of Iran. In 47 of 88 samples (53.4%), the presence of AFM1 was detected in concentrations between 82 ng/kg and 1254 ng/kg. The mean level of AFM1 of positive samples was 412 ng/kg. AFM1 in 28 (31.8%) samples was higher than the maximum tolerance limit (250 ng/kg) accepted by some countries. Statistical analysis showed that there were no significant differences (P>0.05) between the mean concentrations of AFM1 in cheese samples of spring, summer, autumn and winter. However, the mean concentration of AFM1 in cheese samples from spring and summer was significantly lower than autumn and winter (P=0.05). It can be concluded that the high occurrence of AFM1 in cheese is probably due to the presence of aflatoxin in the feed and cheese milk. This condition should be considered as a probable hazard for human health.

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