Isolation and identification of bacteria from mobile phones of students and employees of Hajee Mohammad Danesh Science and Technology University, Bangladesh

2020 ◽  
Vol 6 (3) ◽  
pp. 570-576
Author(s):  
Zakarie Ahmed Hussein ◽  
Md Khaled Hossain ◽  
Nazmi Ara Rumi ◽  
Md Shajedur Rahman ◽  
Tonmoy Kundu ◽  
...  
Keyword(s):  
Mobile Phone ◽  
Mobile Phones ◽  
Disease Transmission ◽  
Biochemical Characterization ◽  
Science And Technology ◽  
Antibiotic Sensitivity ◽  
Personal Hygiene ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Identification Of Bacteria

Microbes are capable to survive on mobile phone surface and serve as a potential transporter of microorganism amongst handlers. Thus, the study focused on isolation and identification of bacteria from mobile phones of academic and non-academic staffs (including students and cleaners) of Hajee Mohammad Danesh Science and Technology University Dinajpur, Bangladesh. A total of 32 swab samples of mobile phones were collected. The bacteria isolates were identified on the basis of morphological, cultural and biochemical characterization. The total viable count (TVC) of different swab samples of mobile phones in different categories were ranged from 73×10-6 CFU/ml to 260×10-6 CFU/ml. Analysis revealed that, among the samples 21 (25.6%) isolates were Staphylococcus spp, 17 (20.7%) were Bacillus spp, 16 (19.5%) were Klebsiella spp, 15 (18%) were Pseudomonas spp and 13 (15.85%) were Salmonella spp. Additionally, antibiotic sensitivity test revealed the bacteria isolates were resistant to Penicillin, Amoxicillin, Cefaclor, Ofloxacin and Ciprofloxacin. The findings suggest that all the samples under observation were highly susceptible to a number of microbes belongs to the natural flora of human body. Hence, it is encouraged to avoid mobile phone handling while eating. Last but not the least, personal hygiene is highly recommended, as mobile phone can be a potential source of disease transmission. Asian J. Med. Biol. Res. September 2020, 6(3): 570-576

scholarly journals Microbial Contamination Analysis of Mobile Phones from certain Users of Chattogram, Bangladesh

2021 ◽  
Vol 14 (4) ◽  
pp. 1550-1556
Author(s):  
Mohammad Zobaidul Alam
Keyword(s):  
Mobile Phone ◽  
Mobile Phones ◽  
Pathogenic Bacteria ◽  
Antibiotic Sensitivity ◽  
Coliform Bacteria ◽  
Personal Hygiene ◽  
Diffusion Method ◽  
Most Probable Number ◽  
Probable Number ◽  
Hospital Patients

Mobile phone is an essential part of everyday life in modern days. Mobile phones act as vehicles for transmitting pathogenic bacteria due to lack of awareness and widespread use. This study aimed to investigate the bacterial contamination of mobile phones of different categories people at Chattogram city, Bangladesh. During the present study, 40 swab samples were collected from the mobile phones of students, businessmen, fishermen, and hospital patients for the isolation, identification of mobile phone associated bacteria, and their antibiogram. In our study, total viable count (TVC) was performed by the pour plate method and total coliform count (TCC) by the most probable number (MPN) method. Besides these, five selective media were used to isolate pathogenic bacteria from mobile phones and then identified. Antibiotic sensitivity assay was performed by disc diffusion method with 10 different antibiotics. Mobile phones of hospital patients (20165 cfu/ml) and students (1578 cfu/ml) showed the highest and lowest TVC respectively. Coliform bacteria were detected from the mobile phones of 100% hospital patients, 90% from both businessmen, and fishermen but only 30% from students. Klebsiella pneumoniae and Pseudomonas aeruginosa were found the most prevalent bacteria but Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus sp., Bacillus sp., E. coli, Salmonella sp., Citrobacter sp., Serratia sp., Proteus sp., and Enterobacter sp. were also detected. Almost all the isolates were highly resistant to ampicillin, amoxicillin, rifampin, erythromycin and sensitive to ciprofloxacin, gentamicin, azithromycin, and tetracycline. Our findings ensure that mobile phones act as an important source of pathogenic organisms for humans and can serve as a vehicle for cross-transmission of microbiota. So, washing hands before and after handling food and also personal hygiene is very important.

scholarly journals ISOLATION AND IDENTIFICATION OF MICROFLORA FROM APPARENTLY HEALTHY CAGED PARROTS OF DHAKA ZOO OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 05-10 ◽  
Author(s):  
J Akhter ◽  
MT Hossain ◽  
MT Islam ◽  
MP Siddique ◽  
MA Islam
Keyword(s):  
Research Work ◽  
Antibiotic Sensitivity ◽  
Biochemical Properties ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Gram Negative ◽  
E Coli ◽  
Apparently Healthy

The research work was conducted to isolate and identify the microflora from apparently healthy caged parrots. A total of 45 samples (oral swabs, cloacal swabs and feces) were collected from five types of caged parrots (Gray cockatiels, Rose ringed parakeet, Alexandriane parakeet, Red breast parakeet and Blossom headed parakeet) of Dhaka Zoo during the period from April to August 2009. The samples were cultured on different bacteriological media and the bacteria were identified by their cultural and biochemical properties. All the isolates were allowed for antibiogram study. The bacteria isolated in this study from different types of caged parrots were E. coli (64.44%), Salmonella spp. (46.67%), Staphylococcus spp. (46.67%), Pasteurella spp. (33.33%), Proteus spp. (6.67%) and some unidentified Gram-positive and Gram-negative bacteria. Of these isolates, E. coli was the most frequent isolate. The frequency of Gram-negative bacteria was higher in this study. The percentage of bacterial isolates recovered from each type of parrots was almost similar. Irrespective of types of parrots, the higher percentage of different bacteria was isolated from cloacal swab (77.78%) followed by feces (75.56%). The 68.89% isolates were recovered from oral swab. All the suspected isolates of Salmonella spp. were confirmed by slide agglutination test using Salmonella polyvalent ‘O’ antiserum. Among the 21 Salmonella spp. isolated in this study, 4 (19.05%) isolates were identified as S. Pullorum when tested with specific antisera against S. Pullorum. The results of antibiotic sensitivity tests revealed that ampicillin and amoxicillin were completely resistant to E. coli and Pasteurella spp.; ampicillin to Proteus spp.; and furazolidone to Salmonella spp. and Pasteurella spp. However, the antibiotics of fluoroquinolone group such as ciprofloxacin, norfloxacin and enrofloxacin showed moderate to high sensitivity against almost all the bacterial isolates. Of these, ciprofloxacin was found to be consistently highly sensitive to all the bacterial isolates. DOI = 10.3329/bjvm.v8i1.8349 Bangl. J. Vet. Med. (2010). 8(1): 05-10

scholarly journals Isolation of Surveillance Pathogenic Fungal Microbial Contaminant on Mobile Phone

2019 ◽  
Vol 7 (20) ◽  
pp. 3493-3496
Author(s):  
Amanah Amanah ◽  
Dadan Ramadhan Apriyanto ◽  
Hikmah Fitriani
Keyword(s):  
Aspergillus Niger ◽  
Mobile Phone ◽  
Aspergillus Flavus ◽  
Mobile Phones ◽  
Disease Transmission ◽  
Microbial Contamination ◽  
Microbial Infection ◽  
Candida Sp ◽  
Fungal Isolates ◽  
Natural Flora

AIM: Mobile phone has been used daily by almost everyone. This Research surveyed microbial contamination of mobile phones in the faculty of Medicine Universitas Swadaya Gunung Jati and identify the most influential fungal microbial species. METHODS: A group of 15 samples was analysed to identify fungal isolates. The mobile phones were swabbed firmly passing its touch screen using sterile swabs then inoculated into media for fungi. Frequency distribution of isolates was calculated. RESULTS: There were fungal isolates as follows: Aspergillus Orchareus, Aspergillus flavus, Alternaria, Aspergillus niger, Penicillium sp., Cladosporium sp., Candida sp., Aspergillus Fumigatus, and Mucor sp. at the rate of 19, 6, 1, 3, 2, 10, 2, 52, 2%, respectively. CONCLUSION: The research indicates that all mobile phones were considerably having microbial infection, mostly from humans’ natural flora and also from the air and soil. This determines that it is necessary to sterilize hands prior to a contact with mobile phones since it could lead into disease transmission.

scholarly journals Isolation and Identification of Bacteria in the Mouth Before and After Ablution

2021 ◽  
Vol 3 (3) ◽  
pp. 132-138
Author(s):  
Fitriah Fitriah ◽  
Mochammad Erwin Rachman ◽  
Sri Wahyuni Gayatri ◽  
Fendy Dwimartyono ◽  
Hasta Handayani Idrus
Keyword(s):  
Oral Health ◽  
The Body ◽  
Personal Hygiene ◽  
Pseudomonas Sp ◽  
Normal Flora ◽  
Isolation And Identification ◽  
Gram Positive Bacteria ◽  
Before And After ◽  
Identification Of Bacteria ◽  
Number Of Bacteria

Background: The oral is the gateway for the entry of various kinds of microorganisms into the body, with the prevalence of people having dental and oral problems in Indonesia increasing every year. The normal flora of the oral acts as a body defense, but it can cause disease due to predisposing factors, namely oral hygiene. Therefore, it is necessary to find an alternative in maintaining oral health. Islam is a religion that emphasizes personal hygiene, such as performing ablution. Content: The types of bacteria found in the oral before ablution was 33.33% Pseudomonas sp., 6.67% Lactobacillus sp., 3.33% Streptococcus sp. and 0.14% Staphylococcus sp. while the types of bacteria found in the oral after ablution were 26,8% Pseudomonas sp., 20% Lactobacillus sp., 5% Streptococcus sp. and 2% Staphylococcus sp. Conclusion: There was a change in the number of bacteria, namely an increase in gram-positive bacteria in the oral after ablution.

scholarly journals BACTERIA IN ANIMAL FEED AND THEIR SUSCEPTIBILITY TO ANTIMICROBIAL DRUGS

2012 ◽  
Vol 5 (2) ◽  
pp. 27-33
Author(s):  
Igor M. Stojanov ◽  
Milica M. Živkov Baloš ◽  
Miloš C. Kapetanov ◽  
Jasna Z. Prodanov Radulović ◽  
Jelena M. Petrović
Keyword(s):  
Animal Feed ◽  
Bacterial Species ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Isolation And Identification ◽  
Antimicrobial Drugs ◽  
Microbiological Safety ◽  
Feed Control ◽  
Identification Of Bacteria ◽  
Feed Safety

Control of microbiological safety of animal feed is regulated by a law. The regulation is based on methodological standards for estimation of feed quality. Those methods are permanently amended and strive for continuous improvement of microbiological safety of feed. The question remains whether the presence or absence of some bacterial species is sufficient for proper assessment of feed safety. The answer to this question can be found not only in the examination of the number of bacteria in animal feed, but also in qualitative analysis of the isolated microorganisms. The sensitivity of bacteria from animal feed to antimicrobials is one of the qualitative characteristics of microorganisms that can affect food safety even if the feed meets the standards and is within the maximum permitted level. The subject of our work was to determine the presence of bacteria in animal feed with a purpose to examine if they possess lower sensibility to antibiotics. For testing antibiotic sensitivity the isolates from routine microbiological feed control were used. The animal feed was analysed by the standard methods for the isolation and identification of bacteria and determination of susceptibility was done using disc diffusion method according to CLSI. The following bacteria were detected: Escherichia coli, Enterobacter sp., Staphylococcus sp., Micrococcus sp., Bacillus cereus, Bacillus sp. The results showed that some isolates have reduced susceptibility to certain antimicrobial drugs (The resistance is different for different antibiotics and microorganism - from 8,33% to 100%). This finding gives a reason for further examination of certain characteristics of the bacteria found in animal feed and to detect their possible influence on ecology.

scholarly journals Antimicrobial resistance profile of Salmonella present in organic farming in Selangor, Malaysia

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2176-2180
Author(s):  
T.Y. Thung ◽  
N. Mazlan ◽  
E. Lee ◽  
C.Y. New ◽  
C.W. Tan ◽  
...  
Keyword(s):  
Organic Farming ◽  
Biochemical Characterization ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Salmonella Spp ◽  
Disk Diffusion Method ◽  
Mar Index ◽  
Resistance To Penicillin ◽  
Susceptibility Profiles ◽  
Vegetable Farm

Presence of Salmonella in organic farming may lead to contamination in fresh produce. This study was designed to detect Salmonella contamination in organic vegetable farm situated in Serdang and to evaluate the antibiotic susceptibility profiles of the isolates. A total of 460 samples of brinjal, cucumber, ladies’ fingers and soil were collected and examined for the presence of Salmonella. The obtained isolates were identified and confirmed by biochemical characterization and serotyping. Antibiotic sensitivity profiles of the isolates were determined by using agar disk diffusion method. Salmonella spp. was detected in brinjal (1.7%, n=2), cucumber (1.7%, n=2), ladies’ fingers (2.5%, n=3) and soil (5.0%, n=5) samples. The prevalent serovars were Salmonella enterica ser. Senftenberg, S. enterica ser. Weltevreden and S. enterica ser. Corvallis. All strains were resistance to penicillin and vancomycin, with multiple antibiotic resistance (MAR) index between 0.21 and 0.36, demonstrated here as multi-drug resistant (MDR) Salmonella. The result highlighted that organic vegetables constituted potential sources of Salmonella, informing continuous monitoring and tightened surveillance are necessary to ensure food safety.

scholarly journals Isolation and Identification of Bacteria Associated with Suya (Roasted Meat Product) Sold in Dutsinma Local Government Area, Kastina State

2019 ◽  
pp. 1-8
Author(s):  
J. B. Orpin ◽  
I. Mzungu ◽  
C. G. Osuji
Keyword(s):  
Safety Issue ◽  
Meat Product ◽  
Personal Hygiene ◽  
Plate Count ◽  
Control Points ◽  
Isolation And Identification ◽  
Critical Control Points ◽  
Clean Environment ◽  
Identification Of Bacteria ◽  
Aerobic Plate Count

A study was carried out to isolate and identify Bacteria associated with suya (roasted meat product) sold in Dutsinma metropolis. Bacteriological analysis was carried out on the thirty (30) unspiced and thirty (30) spiced the samples collected from five (5) different retail outlets for identification and isolation using microscopy and biochemical test. The Prevalence of occurrence of the bacteria isolates was highest for Escherichia coli with 25.9%, Staphylococcus epidermidis with 24.5%, Bacillus cereus with 21.0%, Klebsiella pneumoniae with 11.8%, Staphylococcus aureus with 8.4% Streptococcus faecalis with 6.3%, and Salmonella sp with 2.1%. The mean aerobic plate count were in order of 106 (cfu/g) with the highest value for unspiced suya samples at 2.65 and that of spiced suya samples was 2.95. Occurrence of such organisms in ready-to –eat food constitutes a food safety issue which calls for urgent response in the education of suya producers on the hazards, Critical Control Points and the importance of personal hygiene and clean environment. Critical limits for the critical control points identified in this study are proposed. 

ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH MOBILE PHONES IN SULAIMANI CITY

2016 ◽  
Vol 6 (1) ◽  
pp. 21-27
Author(s):  
Bayan Taha Majid Zmnako ◽  
◽  
Narmeen Nasra AL-Deen Fathi ◽  
Sherko A Omer ◽  
◽  
...  
Keyword(s):  
Mobile Phones ◽  
Isolation And Identification ◽  
Identification Of Bacteria

scholarly journals Identification and characterization of Salmonella spp. from samples of broiler farms in selected districts of Bangladesh

2020 ◽  
Vol 13 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Debashish Mridha ◽  
Md. Nasir Uddin ◽  
Badrul Alam ◽  
A. H. M. Taslima Akhter ◽  
SK. Shaheenur Islam ◽  
...  
Keyword(s):  
Public Health ◽  
Food Safety ◽  
Sampling Technique ◽  
Multidrug Resistant ◽  
Personal Hygiene ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
The Difference ◽  
Public Health Hazards ◽  
Moderate Resistance

Background and Aim: Salmonella spp. are an important group of pathogens responsible for human and animal diseases. This study aimed to estimate the prevalence and identify and characterize of Salmonella spp. isolated from broiler farms of Gazipur, Tangail, and Dhaka districts of Bangladesh. This study also evaluated the difference of Salmonella positivity status between two groups of farms, good practices adapted in broiler rearing at the project intervened farms, and non-project intervened traditional farms. Materials and Methods: A total of 352 samples including 128 cloacal swabs, 32 whole carcasses, 64 feed, 64 water, and 64 attendants' hand rinses were collected through convenient sampling technique from 16 poultry food safety project of Food and Agricultural Organization of United Nations Bangladesh intervened farms and other 16 non-project intervened farms in the same location. Various cultural based techniques and biochemical methods were employed for the estimation of prevalence, isolation, and identification of Salmonella spp. which was further evaluated by polymerase chain reaction. Antimicrobial susceptibility test using disk diffusion methods and serogrouping by slide agglutination test was accomplished for additional characterization. Results: Among the samples, an overall prevalence of Salmonella spp. was 31.25% (110/352) (95% confidence interval [CI]=26.44-36.38%). However, the prevalence of Salmonella spp. was 24.43% (43/176) (95% CI=18.28-31.47) in project intervened farms and 38.07% (67/176) (95% CI=30.87-45.68%) in non-intervened farms. Among the 110 isolates, 31.82% (35/110) were fitted under serogroup B, and the rest of the isolates 75 (68.18%) under serogroup D. Of 110 isolates, 82.72%, 77.27%, 81.82%, and 79.09% were susceptible to ciprofloxacin, gentamycin, norfloxacin, and streptomycin, respectively. In addition, 81.82% and 80% isolates were resistant to erythromycin and tetracycline, respectively. Isolated Salmonella spp. presented moderate resistance to both amoxicillin and azithromycin. Alarmingly, 80.91% (89/110) isolates were shown to be multidrug-resistant Salmonella spp. Conclusion: The study has presented a significant variation of the prevalence of Salmonella spp. between project intervened and non-project intervened farms, and this indicates project intervened farms are comparatively safer than the non-intervened farms considering public health and food safety grounds. This research outcome also has highlighted a substantial proportion of poultry origin multidrug resistance Salmonella spp. is a potential source of public health hazards. In this regard, proper awareness creation and motivational activities on good agriculture practices in poultry rearing and maintaining good personal hygiene at the farmers' level are warranted through participatory training.

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