scholarly journals Isolation and Identification of Bacteria in the Mouth Before and After Ablution

2021 ◽  
Vol 3 (3) ◽  
pp. 132-138
Author(s):  
Fitriah Fitriah ◽  
Mochammad Erwin Rachman ◽  
Sri Wahyuni Gayatri ◽  
Fendy Dwimartyono ◽  
Hasta Handayani Idrus
Keyword(s):  
Oral Health ◽  
The Body ◽  
Personal Hygiene ◽  
Pseudomonas Sp ◽  
Normal Flora ◽  
Isolation And Identification ◽  
Gram Positive Bacteria ◽  
Before And After ◽  
Identification Of Bacteria ◽  
Number Of Bacteria

Background: The oral is the gateway for the entry of various kinds of microorganisms into the body, with the prevalence of people having dental and oral problems in Indonesia increasing every year. The normal flora of the oral acts as a body defense, but it can cause disease due to predisposing factors, namely oral hygiene. Therefore, it is necessary to find an alternative in maintaining oral health. Islam is a religion that emphasizes personal hygiene, such as performing ablution. Content: The types of bacteria found in the oral before ablution was 33.33% Pseudomonas sp., 6.67% Lactobacillus sp., 3.33% Streptococcus sp. and 0.14% Staphylococcus sp. while the types of bacteria found in the oral after ablution were 26,8% Pseudomonas sp., 20% Lactobacillus sp., 5% Streptococcus sp. and 2% Staphylococcus sp. Conclusion: There was a change in the number of bacteria, namely an increase in gram-positive bacteria in the oral after ablution.

EFEKTIVITAS BOOKLET TERHADAP PENGETAHUAN DAN SIKAP PEMELIHARAAN KESEHATAN GIGI MULUT PADA IBU HAMIL

2019 ◽  
Vol 4 (1) ◽  
pp. 66
Author(s):  
Rahmaini Fitri
Keyword(s):  
Oral Health ◽  
Pregnant Women ◽  
The Body ◽  
Wilcoxon Test ◽  
Percentage Increase ◽  
Oral Diseases ◽  
Health Maintenance ◽  
Knowledge And Attitudes ◽  
Before And After ◽  
Knowledge And Attitude

Pregnancy causes physiological changes in the body and as well as in the oral cavity. Dental and oral diseases associated with pregnancy that is, gingivitis, periodontitis and pregnancy granuloma. Mouth dental disease during pregnancy is not only influenced by the pregnancy itself but rather the lack of knowledge about dental and oral health maintenance. Efforts to improve the knowledge of pregnant women about oral health is done by providing information, information necessary for health education media. In this case the media is created and used to improve the knowledge of pregnant women is the booklet. This study aimed to analyze the differences in knowledge and attitudes before and after maintenance booklet oral health in pregnant women. This study is a quasi experiment with one group pre  and post test design. The sample is the first trimester pregnant women who come to the health center in Sentosa Baru Medan as many as 34 people. Analysis of the data  forcompare the average difference in scores of knowledge and attitudes before and after the intervention used the Wilcoxon test. The results showed there is an increased knowledge and attitude maintenance of oral health in pregnant women after being given a booklet with a value of p < 0.001, a percentage increase of 30% knowledge and attitudes percentage of 37%. The conclusions of this study is increased knowledge and attitude maintenance of oral health in pregnant women after being given a booklet.

scholarly journals ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH DECOMPOSING PIG (Sus scrofa) CARCASS

2021 ◽  
Vol 4 (2) ◽  
pp. e126
Author(s):  
Iyabo Victoria Olatubi ◽  
Olukemi Aromolaran ◽  
Samuel Tolani Joseph ◽  
Oluwafeyikemi Ajoke Adeleke
Keyword(s):  
Sus Scrofa ◽  
Skin Surface ◽  
Isolation And Identification ◽  
Forensic Biology ◽  
Gram Positive Bacteria ◽  
Average Temperature ◽  
Baseline Information ◽  
Identification Of Bacteria ◽  
Local Farm ◽  
Staphylococcus Spp

The emerging field of forensic biology has attempted to solve certain problems encountered when estimating post-mortem interval (PMI) by using predictable changes in the microbial and arthropod community structure. Pig (Sus scrofa) carcasses are widely used as animal models in clinical human studies. The objective of this study was to identify bacteria from the skin surface of pig carcass for possible use in forensic investigation. Three pigs (a suitable human substitute) were collected from a local farm and killed by suffocation and further place in a bush land for decomposition. 24hours later skin samples were collected and transported to the laboratory for the isolation of bacteria using standard pour plate techniques and identified using Bergey’s manual of systemic bacteriology. The experiment was conducted in February 2019 during the dry season of the year with an average temperature of 23.50c and relative humidity of 60.8% A total of fourteen (14) isolates were gotten from the pig carcass samples out of which four (4) were Gram-positive bacteria and the remaining ten (10) were Gram-negative. Staphylococcus spp. (28.6%) was the most abundant while Salmonella sp., Serratia sp., Klebsiella sp., Citrobacter sp. and Proteus sp. occurred at 14.3% each. This study focus on the type of bacteria communities during a decomposition process which will help provide baseline information in the application of forensic biology to determination of nature of death, abuse or neglect.

Isolation and Identification of Bacteria from Marine Biofilms

2005 ◽  
Vol 277-279 ◽  
pp. 612-617 ◽  
Author(s):  
Yoo Kyung Lee ◽  
Kae Kyoung Kwon ◽  
Kyeung Hee Cho ◽  
Jae Hyun Park ◽  
Hong Kum Lee
Keyword(s):  
Culturable Bacteria ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Rdna Sequences ◽  
Marine Biofilms ◽  
Antifouling Agent ◽  
Identification Of Bacteria ◽  
Invertebrate Larvae

In the marine environment, biofilms cover most of the subtidal and intertidal solid surfaces. Culturable bacteria forming marine biofilms were isolated on artificial substrate called acrylic coupons. The bacterial isolates were identified through a comparison of 16S rDNA sequences. A total of 115 strains were cultured and identified, 45 of which showed the same sequences with other strains. Therefore, 70 strains were finally identified. The bacterial isolates belonged to a–Proteobacteria (32 isolates), g–Proteobacteria (12 isolates), CFB group bacteria (4 isolates), high GC Gram-positive bacteria (9 isolates), and low GC Gram-positive bacteria (13 isolates). The bacterial isolates may be used as standard bacteria to test new antifouling agent. They may also be utilized as useful bacteria to enhance the settlement of commercial algae and invertebrate larvae for aquaculture.

scholarly journals Isolasi Dan Identifikasi Bakteri Yang Terdapat Pada Saluran Pencernaan Ikan Gurami (Osphronemus gouramy) Dengan Pemberian Pakan Komersil Yang Berbeda [Isolation And Identification Of Bacterial In The Digestive Organ Of Gurami Fish (Osphronemus gouramy) With Different Commercial Feed]

2019 ◽  
Vol 6 (1) ◽  
pp. 87
Author(s):  
Franch Dalahi, Sri Subekti, Agustono
Keyword(s):  
Digestive Tract ◽  
Eating Habits ◽  
Bacterial Flora ◽  
Natural Food ◽  
Pseudomonas Sp ◽  
Isolation And Identification ◽  
Growth And Survival ◽  
Commercial Feed ◽  
Different Types ◽  
Identification Of Bacteria

Abstract Based on the food habits, gurami is omnivorous fish that feed herbivorous carp tend consists of natural food and artificial feed (pellets). The feed is needed to support the growth and survival of Gurami. If feed is given in accordance with the eating habits of Gurami and contain high nutritional Gurami can then growth accelerated faster. Some types of bacteria found in the digestive tract of animals has an important role in order to improve feed utilization, fish health, and environmental quality improvement and microorganisms. In addition, some bacterial flora in the gastrointestinal tract plays an important role and produce several types of enzymes in the digestive tract may contribute to the metabolism of the host. The purpose of this study was to determine the bacteria in the digestive tract of Gurami (Osphronemus gouramy) were given three different types of commercial feed. This study begins with a sampling of Gurami (Oshpronemus gouramy) of maintenance in the Faculty of Fisheries and Marine at Airlangga University in Surabaya and then do stage isolation and identification of bacteria in laboratory bacteria and Microbiology, Faculty of Veterinary Medicine, Airlangga University Surabaya. This study was conducted in July 2013. This research is a descriptive study, the method of solving the problem by describing the subject matter into a form that is easy to understand and based on facts. Results of research on gurami fish that given by three different types of commercial feed, there are two types of bacteria that dominate in the digestive tract of carp, the Pseudomonas sp. and Serratia sp. Both types of bacteria has the potential to help the digestive system of gurami fish. The result of this research are need for further research using the bacterium Pseudomonas sp. and Serratia sp. as probiotics.

scholarly journals Isolasi dan Identifikasi Bakteri Gram Negatif pada Uterus Sapi Aceh yang Mengalami Repeat Breeding

2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Indi Rafika ◽  
Cut Nila Thasmi ◽  
Herrialfian Herrialfian ◽  
Rosmaidar Rosmaidar ◽  
Hafizuddin Hafizuddin
Keyword(s):  
Escherichia Coli ◽  
Nutrient Broth ◽  
Gram Negative Bacteria ◽  
Pseudomonas Sp ◽  
Isolation And Identification ◽  
Gram Negative ◽  
E Coli ◽  
Identification Of Bacteria

ABSTRAK. Penelitian ini bertujuan mengetahui jenis bakteri Gram negatif pada uterus sapi Aceh yang mengalami kawin berulang. Dalam penelitian ini digunakan enam ekor sapi Aceh betina yang terdiri atas tiga ekor sapi fertil (K1) dan tiga ekor yang mengalami kawin berulang (K2, repeat breeding, RB). Pengambilan sampel bakteri dalam uterus sapi dilakukan dengan menggunakan swab steril yang disimpan dalam media nutrient broth (NB) untuk isolasi dan identifikasi jenis bakterinya. Berdasarkan hasil penelitian, baik K1 dan K2, positif terinfeksi bakteri dan diperoleh 16 isolat bakteri di antaranya enam isolat berasal dari K1 dan sepuluh isolat berasal dari K2. Dari 10 isolat bakteri Gram negatif yang ditemukan pada sapi RB terdiri atas Escherichia coli (E. coli) (30,00%), Pseudomonas sp. (30,00%), Enterobacter sp. (20,00%), Klebsiella sp. (20,00%). Disimpulkan bahwa bakteri yang paling banyak menginfeksi uterus sapi RB adalah E. coli dan Pseudomonas sp. (Isolation and identification of gram negative bacteria in uterine Aceh cow with repeat breeding) ABSTRACT. The study determined the type of Gram negative bacteria of the Aceh cattle uterus which has been diagnosed with repeat breeding. The study was conducted on six Aceh cows consists of 3 normal fertile cows (K1) and 3 repeat breeding cows (K2, RB). The bacteria in uterine samples were collected using sterile swab, kept into nutrient broth (NB) for further isolation and identification of bacteria. The result of the study showed that the total of 16 isolates of bacteria was observed in all cows : 6 isolates from group K1 and 10 isolates from group K2. A total of 10 isolates from cattle with repeat breeding consist of Escherichia coli (E. coli) (30.00%), Pseudomonas sp (30.00%), Enterobacter sp (20.0%), and Klebsiella sp (20.0%). It can be concluded that the most bacteria that infect uterine cows with repeat breeding are E. coli and Pseudomonas sp.

scholarly journals Isolation and Identification of Bacteria Associated with Suya (Roasted Meat Product) Sold in Dutsinma Local Government Area, Kastina State

2019 ◽  
pp. 1-8
Author(s):  
J. B. Orpin ◽  
I. Mzungu ◽  
C. G. Osuji
Keyword(s):  
Safety Issue ◽  
Meat Product ◽  
Personal Hygiene ◽  
Plate Count ◽  
Control Points ◽  
Isolation And Identification ◽  
Critical Control Points ◽  
Clean Environment ◽  
Identification Of Bacteria ◽  
Aerobic Plate Count

A study was carried out to isolate and identify Bacteria associated with suya (roasted meat product) sold in Dutsinma metropolis. Bacteriological analysis was carried out on the thirty (30) unspiced and thirty (30) spiced the samples collected from five (5) different retail outlets for identification and isolation using microscopy and biochemical test. The Prevalence of occurrence of the bacteria isolates was highest for Escherichia coli with 25.9%, Staphylococcus epidermidis with 24.5%, Bacillus cereus with 21.0%, Klebsiella pneumoniae with 11.8%, Staphylococcus aureus with 8.4% Streptococcus faecalis with 6.3%, and Salmonella sp with 2.1%. The mean aerobic plate count were in order of 106 (cfu/g) with the highest value for unspiced suya samples at 2.65 and that of spiced suya samples was 2.95. Occurrence of such organisms in ready-to –eat food constitutes a food safety issue which calls for urgent response in the education of suya producers on the hazards, Critical Control Points and the importance of personal hygiene and clean environment. Critical limits for the critical control points identified in this study are proposed. 

Isolation and identification of bacteria from mobile phones of students and employees of Hajee Mohammad Danesh Science and Technology University, Bangladesh

2020 ◽  
Vol 6 (3) ◽  
pp. 570-576
Author(s):  
Zakarie Ahmed Hussein ◽  
Md Khaled Hossain ◽  
Nazmi Ara Rumi ◽  
Md Shajedur Rahman ◽  
Tonmoy Kundu ◽  
...  
Keyword(s):  
Mobile Phone ◽  
Mobile Phones ◽  
Disease Transmission ◽  
Biochemical Characterization ◽  
Science And Technology ◽  
Antibiotic Sensitivity ◽  
Personal Hygiene ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Identification Of Bacteria

Microbes are capable to survive on mobile phone surface and serve as a potential transporter of microorganism amongst handlers. Thus, the study focused on isolation and identification of bacteria from mobile phones of academic and non-academic staffs (including students and cleaners) of Hajee Mohammad Danesh Science and Technology University Dinajpur, Bangladesh. A total of 32 swab samples of mobile phones were collected. The bacteria isolates were identified on the basis of morphological, cultural and biochemical characterization. The total viable count (TVC) of different swab samples of mobile phones in different categories were ranged from 73×10-6 CFU/ml to 260×10-6 CFU/ml. Analysis revealed that, among the samples 21 (25.6%) isolates were Staphylococcus spp, 17 (20.7%) were Bacillus spp, 16 (19.5%) were Klebsiella spp, 15 (18%) were Pseudomonas spp and 13 (15.85%) were Salmonella spp. Additionally, antibiotic sensitivity test revealed the bacteria isolates were resistant to Penicillin, Amoxicillin, Cefaclor, Ofloxacin and Ciprofloxacin. The findings suggest that all the samples under observation were highly susceptible to a number of microbes belongs to the natural flora of human body. Hence, it is encouraged to avoid mobile phone handling while eating. Last but not the least, personal hygiene is highly recommended, as mobile phone can be a potential source of disease transmission. Asian J. Med. Biol. Res. September 2020, 6(3): 570-576

scholarly journals Identification of Staphylococcus Sp bacteria On Mobile And Personal Hygiene Practice Analysis

2018 ◽  
pp. 40-49
Author(s):  
Aulia Rahman ◽  
Ikhram Hardi ◽  
Alfina Baharuddin
Keyword(s):  
Mobile Phones ◽  
Bacterial Growth ◽  
Bacterial Contamination ◽  
Sampling Technique ◽  
Personal Hygiene ◽  
Mobile Users ◽  
Hygiene Practice ◽  
Practice Analysis ◽  
Identification Of Bacteria ◽  
Number Of Bacteria

Mobile users every year are increasing in number. Users, use Mobile regularly throughout the day but do not clean it properly and do not wash their hands as often as possible. This will contribute to the increase of bacterial contamination on Mobile. The purpose of this study is: to determine the effect of knowledge, attitudes and actions of students on personal hygiene and the presence of bacteria on the Mobile and know the number of bacteria on the Mobile on the students. Type of research used observasional analitic Population in this research is FKG UMI students While the sample is a student of FKG UMI semester 5. Sampling technique is pupossive sampling. The result showed that from 15 cellular samples found growth of fungus sp., 5 samples showed no bacterial growth, 10 samples of bacteria Staphylococcus sp were samples in sample code 04D amounted to 2.5x104 Cfu / cm2, sample code 05E amounted to 1.8x103 Cfu / cm2, sample code 06 is 2.9 x 101 Cfu / cm2, the sample code of 10J is 2.3 x 101 Cfu / cm2, the sample code of 11K is 4.1 x 102 Cfu / cm2, the 12L sample code is 2.3x104 Cfu / cm, the 13M sample code is 2.5 x 103 Cfu / cm2, the sample code of 14N is 3.8 x 101 Cfu / cm2, the sample code 15O is 3.9 x 101 Cfu / cm2, and 1 sample with the code 08H is identified Shingomonas paucimobilis bacteria that amounted to 6.5 x 104 Cfu / cm2. Knowledge affects personal hygiene (ρ = 0.02), Attitude does not affect personal hygiene (ρ = 0.064). The action affects personal hygiene (ρ = 0.025). The highest number of bacteria on mobile is 6,5 x 104 Cfu / cm2. Identification of bacteria on mobile that is bacterium Staphylococcus sp. and bacteria Shingomonas paucimobilis. Expected to the students to further improve personal hygiene by washing hands after each use / use of mobile phones.

Influence of Cell Wall Composition on the Fossilisation of Bacteria and the Implications for the Search for Early Life Forms

1997 ◽  
Vol 161 ◽  
pp. 491-504 ◽  
Author(s):  
Frances Westall
Keyword(s):  
Cell Wall ◽  
Life Forms ◽  
Cation Binding ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Transmission Electron ◽  
Hydrothermal Sediments ◽  
Identification Of Bacteria ◽  
The Difference

AbstractThe oldest cell-like structures on Earth are preserved in silicified lagoonal, shallow sea or hydrothermal sediments, such as some Archean formations in Western Australia and South Africa. Previous studies concentrated on the search for organic fossils in Archean rocks. Observations of silicified bacteria (as silica minerals) are scarce for both the Precambrian and the Phanerozoic, but reports of mineral bacteria finds, in general, are increasing. The problems associated with the identification of authentic fossil bacteria and, if possible, closer identification of bacteria type can, in part, be overcome by experimental fossilisation studies. These have shown that not all bacteria fossilise in the same way and, indeed, some seem to be very resistent to fossilisation. This paper deals with a transmission electron microscope investigation of the silicification of four species of bacteria commonly found in the environment. The Gram positiveBacillus laterosporusand its spore produced a robust, durable crust upon silicification, whereas the Gram negativePseudomonas fluorescens, Ps. vesicularis, andPs. acidovoranspresented delicately preserved walls. The greater amount of peptidoglycan, containing abundant metal cation binding sites, in the cell wall of the Gram positive bacterium, probably accounts for the difference in the mode of fossilisation. The Gram positive bacteria are, therefore, probably most likely to be preserved in the terrestrial and extraterrestrial rock record.

Parathyroid gland before and after cisplatin treatment

1987 ◽  
Vol 45 ◽  
pp. 860-861
Author(s):  
S.K. Aggarwal ◽  
J.M. Fadool
Keyword(s):  
Parathyroid Gland ◽  
Wistar Rats ◽  
Antitumor Agent ◽  
The Body ◽  
Cross Linking ◽  
Limiting Factor ◽  
Hormonal Changes ◽  
Primary Mechanism ◽  
Before And After ◽  
Dna Strands

Cisplatin (CDDP) a potent antitumor agent suffers from severe toxic side effects with nephrotoxicity being the major dose-limiting factor, The primary mechanism of its action has been proposed to be through its cross-linking DNA strands. It has also been shown to inactivate various transport enzymes and induce hypocalcemia and hypomagnesemia that may be the underlying cause for some of its toxicities. The present is an effort to study its influence on the parathyroid gland for any hormonal changes that control calcium levels in the body.Male Swiss Wistar rats (Crl: (WI) BR) weighing 200-300 g and of 60 days in age were injected (ip) with cisplatin (7mg/kg in normal saline). The controls received saline injections only. The animals were injected (iv) with calcium (0.5 ml of 10% calcium gluconate/day) and were killed by decapitation on day 1 through 5. Trunk blood was collected in heparinized tubes.

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