scholarly journals Diagnostic Significance of Haemogram Parameters and RBC Indices in Haemoglobin E Trait

2017 ◽  
Vol 8 (2) ◽  
pp. 94-99
Author(s):  
Tarannum Khondaker ◽  
Abul Kashem Jalaluddin Ahmed ◽  
Syed Khairul Amin
Keyword(s):  
Blood Count ◽  
Normal Subjects ◽  
Diagnostic Significance ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Hb E ◽  
Rbc Indices ◽  
Mean Corpuscular Haemoglobin ◽  
The Mean

Background: Hemoglobin E disease are the most common structural haemoglobinopathies, reaching its highest frequency in South East Asia and also prevalent in Bangladesh. There are very few data for effective screening of HB E trait. The aim of the study was to analyze hematological profile and RBC indices as a marker of screening for Hb E trait.Methodology: A cross sectional observational study was conducted among 150 subjects at Dhaka Shishu (Children) Hospital from December 2008 to November 2009. On the basis of Hb electrophoresis, all subjects were divided into three groups. Group I comprised of 50 subjects with Hb E trait, group II comprised 51 subjects with ß thalassemia trait and Group III comprised of 49 normal subjects. Result of RBC indices and blood count were compared between Hb E trait and normal study population.Results: In group I the mean age was 29.53 years and the age range was 2-47 years. In group II the mean age was 30.31 years and the range was 1-55 years. In group III the mean age was 21.81 years and the range was 1-52 years. The present study revealed the mean±SD haemoglobin level (g/dl) in group I (E trait) and group III (normal subjects) were 11.76±1.91 and 10.77±1.4 respectively. Statistically no significant difference was found between these 2 groups (P>.05). The mean corpuscular volume (MCV) in mean±SD in group I and group III were 72.89±12.24 fl and 77.35±10.27 fl respectively. No statistical significance was found as P>0.05. The mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) in group I were 24.84±2.71pg & 32.97±2.27 g/dl and in group III were 28.28±2.71 pg & 32.97±2.38 g/dl respectively. There were no significant difference between group I and III regarding MCH and MCHC.The mean RBC count in E trait and normal subject group were 5.01±71 million/cmm and 4.61±0.65 million/cmm respectively. There was no significant difference was found P>0.05. Mean red cell distribution width (RDW) in group I and III were 14.32 ±2.34 and 14.83±1.28 without any statistical difference (P>0.05)Conclusion: In our study there were no diagnostic significance of Blood count and RBC Indices for detection of Hb E trait. More study should be conducted to find out reliable marker of screening for Hb E carrier.Anwer Khan Modern Medical College Journal Vol. 8, No. 2: Jul 2017, P 94-99

The Role of sFas in the Immune Escape Mechanism and Progression of Cancer Larynx

2009 ◽  
Vol 2 ◽  
pp. CMENT.S2565
Author(s):  
Amr A El Badry ◽  
Ismail Elmofty ◽  
Amira Helmy
Keyword(s):  
Serum Level ◽  
Buffy Coat ◽  
Control Group ◽  
Group Iii ◽  
Soluble Fas ◽  
Group I ◽  
Significant Difference ◽  
Cancer Larynx ◽  
The Mean ◽  
Group Ii

This work assess serum levels of soluble Fas form (sFas) in patients with different stages of laryngeal squamous cell carcinoma(LSCC) to investigate its prognostic significance. We correlate its levels with the morphological changes of peripheral blood cells via buffy coat examinatin. The study population included 70 patients clinically diagnosed and pathohistologically confirmed LSCC in addition to 20 healthy controls. According to TNM classification 33(47.1%) patients were in stage I (group I) and 24(34.3%) in stage II (group II), 13(18.5%) in stage III (group III). The results revealed that the mean serum level of sFas (pg/ml) in the control group was 51.2, in group I was 66.33, in group II was 81.33 and in group III was 112.45. Statistical analysis of the mean of sFas by ELISA test in the patients' groups in comparison to the control revealed a significant increase of both group II and III in comparison to the control group (P < 0.01) but there was no significant difference (P > 0.05) between group I in comparison to either the control or group II. There was a significant difference (P < 0.05) between group I in comparison to group III. LM examination revealed massive extent of the apoptotic cells in group III when compared to both group I and group II. EM examination of the buffy coat revealed apoptotic changes, mainly in the peripheral blood mononuclear cells (PBMNCs), represented by surface membrane ruffles and blebs with clumped nuclear chromatin and vacuolated cytoplasm. In conclusion, this study may help us to better understand one of the escape mechanisms in cancer larynx. This mechanism is represented by the significant increase in both the serum level of sFas and the morphological apoptotic changes that detected in PBMNCs. Soluble Fas may contribute to the progression of laryngeal cancer. It can be used as an attractive target for anticancer therapy and may be considered as a marker of disease progression and poor prognosis in laryngeal cancer.

scholarly journals Effects of daily curve and age on intraocular pressure in Criollo horses

2021 ◽  
pp. 2049-2056
Author(s):  
Ângela Beatriz de Oliveira Bacchin ◽  
◽  
Géssica Maria Ribeiro da Silva ◽  
Maiara Poersch Seibel ◽  
Alessandra Fernandez da Silva ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Age Group ◽  
Slit Lamp ◽  
Group Iii ◽  
Indirect Ophthalmoscopy ◽  
Group I ◽  
Schirmer Tear Test ◽  
Significant Difference ◽  
The Mean ◽  
Made In

The objective of this study was to evaluate the intraocular pressure (IOP) of healthy Criollo horses using a rebound tonometer throughout the day. In addition, assessments were made in horses of different ages. Twenty-seven horses, male and female, were divided into three groups by age: Group I (3-5 years old), Group II (6-8 years old), and Group III: (9-16 years old). Ophthalmic examinations were performed using the Schirmer tear test, slit-lamp biomicroscopy, fluorescein test and indirect ophthalmoscopy. Seven measurements of IOP were assessed on the same day (at 6:00 am, 9:00 am, 12:00 am, 3:00 pm, 6:00 pm, 9:00 pm and 00:00 pm). A t-test was used when there were two groups of comparisons and ANOVA was used to detect differences in IOP between measurement times and between age categories. The average IOP was 28.4 ± 3.7 mmHg for all eyes. The mean IOP for Groups I, II and III were 29.2 ± 3.5, 28.4 ± 4.3 and 27.7 ± 3.2 mmHg, respectively. There was no statistically difference between right and left eyes. There was a significant difference between Group I and Group III (P = 0.008). There were no statistically significant differences between measurements recorded at different times of the day (P = 0.560). The IOP was not influenced by the circadian rhythm, but older horses showed reduced IOP.

scholarly journals Processing and Interpretation Times of CT Angiogram and CT Perfusion in Stroke

2005 ◽  
Vol 32 (4) ◽  
pp. 483-486 ◽  
Author(s):  
Ashok Srinivasan ◽  
Mayank Goyal ◽  
Cheemun Lum ◽  
Thanh Nguyen ◽  
William Miller
Keyword(s):  
Acute Stroke ◽  
Ct Perfusion ◽  
Group Iii ◽  
Ct Angiogram ◽  
Group I ◽  
Or Groups ◽  
Significant Difference ◽  
The Mean ◽  
Time Required ◽  
Mean Time

ABSTRACT:Objective:To determine the mean time for acquiring computed tomogram perfusion (CTP) and CT angiogram (CTA) images in acute stroke. To determine and compare processing and interpretation times amongst three groups of radiologists with varying degree of expertise: two radiology residents (Group I), two neuroradiology fellows (Group II) and four consultant neuroradiologists (Group III).Methods:The mean time of acquisition of CTA and CTP studies was calculated among ten patients presenting with acute stroke. All readers had to process the CTA and CTP images, interpret them (for presence or absence of thrombus and penumbra) and save them on the GE Advantage Windows workstation. The mean time for processing and interpreting these studies was calculated.Results:The mean time for acquisition of CTA and CTP studies in the ten patients was 14.6 ± 5.9 minutes. The time taken for CTA processing and interpretation in Groups I, II and III was 2.3 ± 1.3 min, 1.6 ± 0.4 min and 1.5 ± 0.7 min respectively. The time required for CTP processing and interpretation by the same groups was 5.2 ± 1.7 min, 4.5 ± 1.5 min and 4.1 ± 1.1 min respectively. There was a statistically significant difference of means between Groups I and III in the CTA and CTP processing and interpretation times (p=0.02, p=0.01 respectively) but no statistical difference between Groups I and II (p=0.15, p=0.22 respectively) or Groups II and III (p=0.31, p=0.30 respectively).Conclusion:The CTA and CTP studies can be performed, processed and interpreted quickly in acute stroke.

scholarly journals Non-Dose-Dependent Changes in Liver Enzyme Levels of Children With Epilepsy on Treatment With Sodium Valproate

Dose-Response ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 155932582091844
Author(s):  
Aduragbenro Deborah A. Adedapo ◽  
Winifred Eseoghene Demaki ◽  
IkeOluwa Lagunju
Keyword(s):  
Sodium Valproate ◽  
Transaminase Level ◽  
Significant Negative Correlation ◽  
Liver Function Tests ◽  
Gamma Glutamyl Transferase ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
The Mean ◽  
Glutamyl Transferase

Background: Sodium valproate (VPA) is considered as the drug of choice for the treatment of generalized epilepsy in children. Sodium Valproate may be hepatotoxic. Aim: To assess the level of derangement of liver enzymes in children with epilepsy on treatment with sodium valproate. Methods: A cohort study. One hundred fifty-three children, comprising 51 with epilepsy on treatment with VPA (group I), 51 with epilepsy on treatment with other antiepileptic drugs (AEDs) but not VPA (group II), and 51 with nonconvulsive disorders (group III) had liver function tests performed for them. Data were analyzed by SPSS version 23.0. Results: There were 85 males and 68 females, aged 6 months to 14 years (median = 7.0 years). There was no significant difference in the mean plasma levels of alanine transaminase (ALT), alkaline phosphatase, and gamma glutamyl transferase across the three groups of children. The mean aspartate transaminase level was significantly higher in children in group III. There was a statistically significant negative correlation between the duration of AED therapy and the mean serum level of AST ( r = −0.266, P = 0.016). The serum ALT level showed a statistically significant positive correlation with the duration of AED therapy ( r = 0.268, P = 0.015). Conclusion: Sodium valproate monotherapy does not appear to be associated with significant hepatotoxicity in children in our cohort.

scholarly journals Evaluation of Fracture Resistance of Orthodontic Mini-implants in the Transmucosal Profile Region

2015 ◽  
Vol 16 (5) ◽  
pp. 372-375 ◽  
Author(s):  
Adelson Mota de Aguiar ◽  
Arilton Mota de Aguiar ◽  
Célia Regina Maio Pinzan-Vercelino ◽  
Fausto Silva Bramante
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
Significance Level ◽  
Group Iii ◽  
Mini Implants ◽  
Group I ◽  
Significant Difference ◽  
The Mean ◽  
Group Ii

ABSTRACT Aim This study sought to compare the fracture resistance of three trademarked orthodontic mini-implants in the transmucosal profile region. Thirty-six mini-implants of three different brands, separated into groups I, II and III, were tested. Each group consisted of 12 mini-implants of 6 mm in length. The mean diameter and length of the transmucosal profile of the mini-implants were 1.90 and 2.0 mm in group I, 1.77 and 1.0 mm in group II and 1.50 and 1.0 mm in group III, respectively. The tests were performed on a universal testing machine in compression mode, with a 2,000 kgf load, a speed of 4.0 mm per minute and a chisel-shaped active tip, which acted crosssectionally on the transmucosal profile. Single-criterion analysis of variance was used to compare the three brands. A significance level of 5% and test power of 80% were adopted. The mean fracture resistance achieved by the mini-implants was 172.03 ± 25.59 N for group I, 162.35 ± 30.81 N for group II and 139.69 ± 42.99 N for group III. There was no statistically significant difference in mean fracture resistance among the tested mini-implant brands. Conclusion The transmucosal profile diameter does not seem to be a deciding factor in the choice of mini-implants to minimize the risk of fractures. Clinical significance Although being an in vitro study it is possible to believe that this new brand has a very satisfactory resistance to fracture and enables its use with great efficiency. How to cite this article de Aguiar AM, Bramante FS, de Aguiar AM, Pinzan-Vercelino CRM. Evaluation of Fracture Resistance of Orthodontic Mini-implants in the Transmucosal Profile Region. J Contemp Dent Pract 2015;16(5):372-375.

A COMPARISON OF TWO METHODS FOR MEASUREMENT OF ALDOSTERONE SECRETION RATE IN MAN

1966 ◽  
Vol 53 (2) ◽  
pp. 177-188 ◽  
Author(s):  
P. Lund-Johansen ◽  
T. Thorsen ◽  
K. F. Støa
Keyword(s):  
Urinary Excretion ◽  
Normal Subjects ◽  
Secretion Rate ◽  
Aldosterone Secretion ◽  
Simple Method ◽  
Mean Values ◽  
Pathological Conditions ◽  
Significant Difference ◽  
Derivative Method ◽  
The Mean

ABSTRACT A comparison has been made between (A), a relatively simple method for the measurement of aldosterone secretion rate, based on paper chromatography and direct densitometry of the aldosterone spot and (B) a more elaborate isotope derivative method. The mean secretion rate in 9 normal subjects was 112 ± 26 μg per 24 hours (method A) and 135 ± 35 μg per 24 hours (method B). The »secretion rate« in one adrenalectomized subject after the intravenous injection of 250 μg of aldosterone was 230 μg per 24 hours (method A) and 294 μg per 24 hours (method B). There was no significant difference in the mean values, and correlation between the two methods was good (r = 0.80). It is concluded that the densitometric method is suitable for clinical purposes as well as research, being more rapid and less expensive than the isotope derivative method. Method A also measures the urinary excretion of the aldosterone 3-oxo-conjugate, which is of interest in many pathological conditions. The densitometric method is obviously the less sensitive and a prerequisite for its use is an aldosterone secretion of 20—30 μg per 24 hours. Lower values are, however, rare in adults.

Role of Cord Blood Carboxyhemoglobin in Detecting Significant Hyperbilirubinemia in Term Neonates with ABO Alloimmunization

2021 ◽  
Author(s):  
Mahir Tıraş ◽  
Emrah Can ◽  
Şahin Hamilçıkan
Keyword(s):  
Cord Blood ◽  
Total Serum ◽  
Healthy Controls ◽  
Term Neonates ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
Positive Direct ◽  
Severe Hyperbilirubinemia

Objective This study aimed to assess whether cord blood carboxyhemoglobin (COHb) levels in jaundiced term neonates with and without a positive direct Coombs test (DCT) and in healthy controls could be used as a predictor of severe hyperbilirubinemia. The percentage of cord blood COHb should be higher among neonates with Coombs-positive ABO hemolytic disease than among those with Coombs-negative ABO incompatibility and higher than that of ABO-compatible control neonates. Study Design This cross-sectional descriptive study of 198 term neonates comprised three subgroups: group I featured 68 DCT-positive ABO-incompatible neonates (ABO + DCT), group II featured 60 DCT-negative ABO-incompatible neonates with hyperbilirubinemia (ABO–DCT), and group III featured 70 healthy controls. COHb was determined by an OSM3 hemoximeter. Results Group I differed from groups II and III for cord blood bilirubin, cord blood hemoglobin, and cord blood hematocrit. Groups I and II had higher mean total serum bilirubin (TSB) levels than group III, while there was no difference in the mean TSB levels between groups I and II. There was no significant difference between the COHb group means for groups I, II, and III (p = 0.98). The area under the receiver operating characteristic curve calculated for group I/group III and group II/group III were found to be 0.62 and 0.54, respectively. Conclusion COHb levels did not prove to be superior to the DCT for predicting the risk of developing severe hyperbilirubinemia in term neonates. Key Points

Genoprotective role of vitamin E and selenium in rabbits anaesthetized with sevoflurane

2004 ◽  
Vol 23 (8) ◽  
pp. 413-419 ◽  
Author(s):  
Cetin Kaymak ◽  
Ela Kadioglu ◽  
Hulya Basar ◽  
Semra Sardas
Keyword(s):  
Dna Damage ◽  
Vitamin E ◽  
Comet Assay ◽  
Antioxidant Supplementation ◽  
Group Iii ◽  
Sevoflurane Anaesthesia ◽  
Group I ◽  
The Mean ◽  
Group Ii

In this study, genotoxic effects of repeated sevoflurane anaesthesia were investigated in rabbits with or without antioxidant supplementation. Twenty-one New Zealand male rabbits were included in the study and randomized into three groups as: placebo treated (Group I), vitamin E supplemented (Group II) and selenium supplemented (Group III). Vitamin E and selenium were given intraperitoneally for 15 days before anaesthesia treatment. Anaesthesia was administered using 3% sevoflurane in 4 L/min oxygen for a 3-hour period and continued for 3 days. Blood samples were collected before anaesthesia (Sample 1), after the first, second and third days of sevoflurane administration (Sample 2, Sample 3 and Sample 4 respectively) and the last samples were taken 5 days after the last sevoflurane administration (Sample 5). Genotoxic damage was examined using the comet assay. The degree of damage is assessed by grading the cells into three categories of no migration (NM), low migration (LM) and high migration (HM) depending on the fraction of DNA pulled out into the tail under the influence of the electric field. The number of comets in each sample was calculated (1 × number of comets in category NM + 2 × number of comets in category LM + 3 ×number of comets in category HM) and expressed as the total comet score (TCS), which summarizes the damage frequencies. In Group I, a significant increase in the mean TCSs was observed for Samples 3 and 4 as compared with Sample 1. However, there were no significant differences between Samples 1, 2 and 5. The mean TCS of Sample 4 was significantly higher than Sample 1, 2 and 3 in Group II. Group III demonstrated no significant mean TCSs for any experimental conditions. Statistical differences were also observed between the groups with significant P values. This experimental study points out the presence of DNA damage with repeated sevoflurane anaesthesia and the genoprotective role of antioxidant supplementation on DNA damage in mononuclear leukocytes of rabbits by highly sensitive comet assay.

Bone mineral density and spongiosa architecture in correlation to vertebral body insufficiency fractures

1998 ◽  
Vol 39 (5) ◽  
pp. 538-542 ◽  
Author(s):  
R. Andresen ◽  
S. Radmer ◽  
D. Banzer
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Vertebral Body ◽  
Unknown Etiology ◽  
Insufficiency Fractures ◽  
Mineral Density ◽  
Group Iii ◽  
Group I ◽  
The Mean ◽  
Group Ii

Objective: the clinical value of spinal quantitative CT (sQCT) and the structural patterns of the vertebral bone were studied Material and Methods: sQCT was performed on 246 patients with a mean age of 57 years for whom conventional lateral radiographies of the thoracic and lumbar spine were available. All patients were suffering from back pain of unknown etiology. the bone mineral density (BMD) of the midvertebral section of 3 lumbar vertebral bodies was determined by means of single-energy-(SE)-weighted QCT (85 kV). Spongiosa architecture and density profile analyses were made in the axial images. This was contrasted to BMD values ascertained in SE QCT. the mean BMD was compared to the number of fractures and the patients were divided into three groups: group I — no fracture; group II — one fracture; and group III 1 fracture Results: the mean BMD was: 134.3 (74.1–187.5) mg hydroxyapatite (HA)/ml in group I; 79.6 (58.6–114.3) mg HA/ml in group II; and 52.4 (13.1–79.1)mg HA/ml in group III. A significant deterioration in spongiosa structure was found with increasing demineralization: strongly rarefied patterns predominated in the fracture groups II and III Conclusion: sQCT provides a good risk assessment of the occurrence of vertebral body insufficiency fractures

Seasonal variations in wool growth and heat tolerance of sheep. I. Wool growth

1960 ◽  
Vol 11 (1) ◽  
pp. 75 ◽  
Author(s):  
M Wodzicka
Keyword(s):  
Body Weight ◽  
Day Treatment ◽  
The Other ◽  
Seasonal Rhythm ◽  
Group Iii ◽  
Wool Growth ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
The Difference

The monthly wool growth of three groups of rams was studied at Beltsville, Maryland. Group I received natural daylight (at 38° 53' N.) and was shorn monthly. Group II had a 7:17 hours of daylight to hours of darkness rhythm and was shorn every 6 months, once in winter and once in summer. Group III received natural daylight and was likewise shorn every 6 months. The rams of all groups produced more wool in summer than in winter. This difference was significant (P<0.001). The mean body weight and food intake were both greater in the winter months, which indicated that the seasonal rhythm of wool growth was not a consequence of poorer feeding in winter. The rams which were shorn monthly (group I) grew considerably more wool than the other two groups, but the difference was not statistically significant. The short-day treatment of group II did not increase the annual wool production nor decrease the seasonal rhythm of wool growth. The balance of evidence from this and other experiments indicates that temperature rather than light controls the seasonal rhythm of wool growth.

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