scholarly journals Prevalence of Extended Spectrum B-Lactamases in Hospitalized Patents and Community Patients

2019 ◽  
Vol 5 (2) ◽  
pp. 61-64
Author(s):  
Md Badrul Islam ◽  
Md Abdullah Yusuf ◽  
Samia Afrin ◽  
Md Abul Bashar
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Hospitalized Patients ◽  
Urine Samples ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Medical College ◽  
National Medical ◽  
Community Patients

Objectives: This study was carried out to detect extended spectrum B-lactamases (ESBLs) among Gram negative bacteria isolated from hospitalized patients and community patients (OPD) by double disc synergy test and phenotypic confirmatory test. Methodology: This cross-sectional, prospective study was carried out in the Department of Microbiology. Dhaka National Medical College, over a period of 1 (one) year 2016. Urine samples were collected from patients. Urine samples were from hospitalized patients and community patients. Samples were collected from in-patient and outpatient department of Dhaka National Medical College Hospital having clinical symptoms of microbial infection. Samples were collected from both sexes and different age groups. Result: Total 220 urine samples were collected from suspected cases of urinary tract infection. Total 132 (60%) Gram negative bacteria were isolated from these patients as causative agents. Among the isolates, 88 (75.86%) in hospitalized patients and 44 (42.31%) in community patients were isolated. Out of 132 Gram negative bacteria, 31 (23.48%) were ESBL producers. The percentage of ESBL producing bacteria was (31.81%) in hospitalized patients and (6.82%) in community patients. Conclusion: In the present study, it was observed that considerable numbers of ESBL producing bacteria were detected from urinary tract infection cases. These cases indicate ESBLs will be major threat for antibiotic therapy. Bangladesh Journal of Infectious Diseases, December 2018;5(2):61-64

scholarly journals Frequency and Distribution of Gram Negative Bacteria among Hospital and Community Acquired UTI Patients

2015 ◽  
Vol 1 (2) ◽  
pp. 24-26
Author(s):  
Md. Badrul Islam ◽  
Md. Abdullah Yusuf ◽  
Md. Shahjahan Chowdhury ◽  
AFM Arshaedi Sattar ◽  
Samia Afrin
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples ◽  
Gram Negative Bacteria ◽  
Klebsiella Species ◽  
Gram Negative ◽  
Pseudomonas Species ◽  
Community Patients

Background: Gram negative bacteria create a great problem during the treatment of urinary tract infection patients. Objective: This study was undertaken to determine the frequency and distribution of Gram negative bacteria among the UTI patients. Methodology: This cross sectional study was carried out in the Department of Microbiology at Sir Salimullah Medical College, Dhaka from June 2007 to May 2008 for a period of 1(one) year. All the patients presented with the clinically suspected UTI at any age with both sexes were selected as study population. Patients who were hospitalized for at least 2 days or more received different antibiotics were designated as hospital acquired UTI; on the other hand patients who were attended in OPD for the first time were considered as community acquired UTI patients. All urine samples were inoculated in Blood agar and MacConkeys agar media. Gram negative bacteria were isolated and identified by colony morphology, Gram staining and biochemical tests. Result: A total of 220 urine samples were collected from patients suspected to urinary tract infections of which 116 samples were from hospitalized patients and 104 samples were from community patients. Among 220 samples, 132(60.0%) Gram negative bacteria were isolated of which 88(66.7%) isolates were from hospitalized acquired UTI and 44(33.3%) bacteria were isolated from community acquired UTI patients. The difference was statistically significant (p=0.0001). In hospitalized patients out of 88(75.9%) isolated Gram negative bacteria, 67(76.1%) isolates were Escherichia coli, 10(11.4%) isolates were Klebsiella species, 5(5.7%) isolates were Proteus species and 6(6.8%) isolates were Pseudomonas species. Among the isolated bacteria 44(42.3%) bacterial isolates were from community patients of which 36(81.8%) isolates were Escherichia coli, 4(9.1 %) isolates were Klebsiella species, 2(4.5%) isolates were Proteus species and 2(4.5%) bacteria were Pseudomonas species. Conclusion: In the present study, it was observed that considerable numbers of Gram negative bacteria were detected from urinary tract infection cases.Bangladesh Journal of Infectious Diseases 2014;1(2):24-26

scholarly journals C - reactive protein and urinary tract infection due to Gram-negative bacteria in a pediatric population at a tertiary hospital, Mwanza, Tanzania

1970 ◽  
Vol 19 (4) ◽  
pp. 3217-3224
Author(s):  
Martha F Mushi ◽  
Vaileth G Alex ◽  
Mwanaisha Seugendo ◽  
Vitus Silago ◽  
Stephen E Mshana
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Enteric Bacteria ◽  
Gram Negative Bacteria ◽  
C Reactive Protein ◽  
Gram Negative ◽  
Reactive Protein ◽  
Significant Bacteriuria ◽  
Tract Infections

Introduction: Gram-negative bacteria are the major cause of urinary tract infections (UTI) in children. There is limited data on UTI systemic response as measured using C-reactive protein (CRP). Here, we report the association of CRP and UTI among children attending the Bugando Medical Centre, Mwanza, Tanzania.Methods: A cross-sectional study was conducted between May and July 2017. Urine and blood were collected and processed within an hour of collection. Data were analyzed using STATA version 13.Results: Of 250 enrolled children, 76(30.4%) had significant bacteriuria with 56(22.4%, 95%CI; 11.5-33.3) having gram-negative bacteria infection. There was dual growth of gram-negative bacteria in 3 patients. Escherichia coli (32.2%, 19/59) was the most frequently pathogen detected. A total of 88/250(35.2%) children had positive CRP on qualitative assay. By multinomial logistic regression, positive CRP (RRR=4.02, 95%CI: 2.1-7.7, P<0.001) and age ≤ 2years (RRR=2.4, 95%CI: 1.23-4.73, P<0.01) significantly predicted the presence of significant bacteriuria due to gram-negative enteric bacteria. Conclusion: C-reactive protein was significantly positive among children with UTI due to gram-negative bacteria and those with fever. In children with age ≤ 2 years, positive CRP indicates UTI due to gram-negative enteric bacteria.Keywords: C - reactive protein, urinary tract infection, Gram-negative bacteria, Mwanza, Tanzania.

scholarly journals Isolation, Identification and Antibiotic Susceptibility Pattern of Urinary Tract Infection Bacterial Isolates

2021 ◽  
Vol 10 (4) ◽  
pp. 2820-2830
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern ◽  
Gram Negative ◽  
Gram Positive Bacteria

Recently, the world's problem has become in the spread of microbes and the extent of their impact on public health, especially the problem of urinary tract infections (UTIs). Therefore, the purpose of the study was to clarify the extent of infection of the urinary tract and the extent of development of these microbes in resistance to antibiotics (antibiotic susceptibility pattern). The pathogens are isolated from hospitals and private medical centers. In this study, two hundred twenty-five urine samples were collected from various sources, where 126 patients were infected with bacteria while 99 patients had no infection. In Gram-negative bacteria, the most common bacteria were E. coli (55.5%) followed by Klebsiella spp (23.0%), Proteus spp (7.14%), Pseudomonas spp (6.34%), and Acinetobacter spp (3.96%). In the Gram-positive bacteria Staphylococcus spp (2.38%) and Enterococcus spp (1.58%) were found. Antibiotic susceptibilities for Gram-negative and positive bacteria were investigated according to clinical laboratory standard institute(CLSI 2019). In Gram-negative bacteria, Amikcin, Impinem, and levofloxacin were sensitive. However, in Gram-positive bacteria, levofloxacin, ofloxacin, and linezolid were sensitive. At the same time, most of the remaining antibiotics are 100% resistant to pathogenic-bacterial isolates. This study showed bacteria that are isolated from urine which causing urinary tract infection and showed resistance to almost all antibiotics, so UTI is difficult to treat by common antibiotics.

scholarly journals Garlic as Alternative Therapy to Treat Uropathogene Bacteria in Women with Urinary Tract Infection in Lomé, Togo

2018 ◽  
Author(s):  
Banfitebiyi Gambogou ◽  
Abdoul Karim Ouattara ◽  
Essodolom Taale ◽  
Simplice D. Karou ◽  
Yaovi A. Ameyapoh ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Alternative Therapy ◽  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Microdilution Method ◽  
Antibiotics Susceptibility ◽  
Time Kill

The urinary tract infection (UTI) is the most common bacterial infection, especially in women. The increased incidence of UTIs, at the last decades have paralleled with the growing emergence of antibiotic resistance. The aim is to evaluate aqueous garlic extract (AGE) susceptibility against multidrug-resistant (MDR) bacteria isolated in urine of women. The investigation of antibacterial propriety and time kill effect of AGE was performed by the well method, microdilution method and spectrophotometer assay. Antibiotics susceptibility assay revealed that the nine MDR bacteria had high resistance against Amoxicillin/ clavulanic acid (100%) and Erythromycin (100%), Cefotaxime (83.33%) and Ceftazidime (83.33%). AGE exhibited potent antibacterial activity against the nine MDR bacteria tested. In Gram-negative bacteria, the inhibition diameters ranged from 20 &plusmn; 3 to 32 &plusmn; 4 mm, with Minimum Inhibitory Concentrations (MICs) ranging from 10% to 12.5% (w/v) and Minimum Bactericidal Concentration (MBCs) was 12.5 % (w/v). Gram-positive bacteria exhibited diameters ranging from 38 &plusmn; 2 to 45 &plusmn; 1 mm; MIC and MBC values ranged from 05 to 10 % (w/v) and were found more susceptible than Gram-negative bacteria. To conclude, this investigation shown that AGE have high potential antibacterial to use as an alternative to treat women UTIs.

Oxolinic acid (prodoxol) for urinary tract infection

1975 ◽  
Vol 13 (18) ◽  
pp. 70-71
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Nalidixic Acid ◽  
Oxolinic Acid ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Staph Aureus ◽  
Wide Range ◽  
Low Incidence

Oxolinic acid (Prodoxol - Warner) is an antimicrobial drug chemically related to nalidixic acid (Negram - Winthrop). The advantages claimed are that it is active against a wide range of Gram-negative bacteria and some strains of Staph. aureus, has a low incidence of unwanted effects and needs to be given only twice daily.

Antimicrobial Activity of Herbal Mixture Extract Combination on Microorganisms Isolated from Urinary Tract infection

Bionatura ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1346-1351
Author(s):  
Risala H Allami ◽  
Raghad S. Mouhamad ◽  
Sura A. Abdulateef ◽  
Khlood abedalelah al-Khafaji
Keyword(s):  
Urinary Tract Infection ◽  
Antibacterial Activity ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Klebsiella Spp

Urinary tract infection (UTI) is the second most common infection after respiratory tract infection. Its prevalence is more in women as compared to men. Approximately 50% of women have an infection of the Urinary tract in their life-time. The bacterial infection is one of the most important bioactivity; using their ability to imitate evanish then distributes international fitness problems into the 21st centenary. Thus a recent study was undertaken to investigate the antibacterial activity of a mixture of three medicinal plants against UTI infectious isolates. The three considered plants were (Aloe vera, Artemisia herba alba and Teucrium polium), which were used in Iraqi medicine for many centuries. The effectiveness of this combination was investigated using in vitro well diffusion method. The extract was tested against four isolated pathogenic bacteria (Staphylococcus aureus, Klebsiella spp, and Proteus spp). The aqueous extract exhibited antibacterial activity against gram-positive and gram-negative bacteria. The mixture extract had the highest effect against S. aureus and Proteus spp, followed by a lower effect on Klebsiella spp. In conclusion, the antibacterial effect of the tested plant extracts confirmed a higher impact on Gram-positive bacteria as compared to Gram-negative bacteria. Therefore, it can be concluded that the usage of these plants as a traditional medicine form can be considered as a strong assistant to regular medicine drugs and treatments.

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