Aerobic Bacterial Pattern in Puerperal Sepsis

Puerperal sepsis is an important cause of maternal morbidity and mortality in developing countries. This study was undertaken to isolate and identify the Aerobic bacterial agents of Puerperal sepsis among the patients admitted in Mymensingh Medical College Hospital during the period from July, 2006 to June, 2007. Endocervical swabs/secretions were collected from 50 cases of Puerperal sepsis and were cultured aerobically. Out of 50 samples, 42 (84%) yielded growth in cultures. Among 42 culture positive cases, 20 (40%) were Aerobic organisms and 22 (44%) were mixed, i.e., Aerobic and Anaerobic bacteria. The isolated Aerobic organisms were Staphylococcus aureus (26, 61.90%), Escherichia coli (6, 14.28%), Staphylococcus epidermidis (5, 11.90%), Streptococcus pyogenes (3, 7.14%) and Enterococcus faecalis (2, 4.76%). The organisms were identified by standard biochemical tests. All isolates of S.aureus and S. epidermidis were sensitive to Cephalexin and Vancomycin. Similarly all the strains of E. coli were sensitive to Gentamicin, Amikacin, Ciprofloxacin and Cephalexin. All the strains of S. pyogenes were sensitive to Amoxicillin and all the isolates of Enterococcus were sensitive to Amoxicillin and Cephalexin.Bangladesh J Med Microbiol 2008; 02 (01): 22-27DOI:http://dx.doi.org/10.3329/bjmm.v2i1.21785

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Antibiotic Sensitivity Pattern of Uropathogens at Khwaja Yunus Ali Medical College Hospital

KYAMC Journal ◽

10.3329/kyamcj.v6i2.33737 ◽

2017 ◽

Vol 6 (2) ◽

pp. 610-613

Author(s):

Abdullah Akhtar Ahmed ◽

Ahmed Nawsher Alam ◽

Rejwana Fatmi ◽

Md Mashiur Rahman ◽

Marufa Sultana Munia ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Medical College Hospital ◽

Urine Samples ◽

College Hospital ◽

Health Care Center ◽

E Coli ◽

Medical College ◽

Staphylococcus Saprophyticus ◽

Sensitivity Pattern ◽

Culture Positive

Increasing resistance among urinary tract pathogens to conventional drugs is found alarming worldwide. The aim of this study was to obtain data on susceptibility patterns of isolated uropathogens from urine samples of patients attending at Khwaja Yunus Ali Medical College Hospital, Enayetpur, Sirajgonj, Bangladesh a tertiary health care center of remote area to currently used antimicrobial agents. A total of 656 urine samples were studied of which 163 (24.8%) were culture positive. Among 163 culture positive cases all uropathogenic isolates were identified. Among the isolates, E. coli was the most predominant 116 (71.1%) followed by Pseudomonas aeruginosa 29 (17.8%). Staphylococcus saprophyticus 12 (7.4%) and Klebsiella pneumoniae 4 (2.4%) and Acenatobacter sp. 2 (1.2%). The majority of isolated E. coli were sensitive to Meropenam (58.6%) and Amikacin (57.7%) followed by gentamicin (52.6%), amoxiclav (43.1%), ciprofloxacin (42.2%). Sensitivity & resistance rate in Staphylococcus saprophyticus were same (50%) in Cephradine . Where complete (100%) resistanc e was shown to Ampicillin in Pseudomona s aeruginosa , Klebsiella pneumoniae and Acenatobacter sp. However most of isolates were poorly sensitive to ampicillin (1.7%), erythromycin (2.5%), cefuroxin (3.4%) and amoxicillin (9.5%). This area-specific monitoring studies aimed to gain knowledge about the type of pathogens responsible for UTIs and their resistance patterns may help the clinician to choose the right empirical treatment. Meropenem, amoxiclav, amikacin, gentamicin, second-generation of both cephalosporins and quinilones are found the most sensitive against the common uropathogens which might be used in the treatment of UTI.KYAMC Journal Vol. 6, No.-2, Jan 2016, Page 610-613

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Bacteriological Etiology of Flood Affected Diarrhoeal Patients Admitted in Dhaka Medical College Hospital and Antibiogram of the isolates

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v2i1.21929 ◽

2016 ◽

Vol 2 (1) ◽

pp. 13-17

Author(s):

Abdur Rahman ◽

Aliya Rashid ◽

Shaheen Ara Begum ◽

Aktheruzzaman Chowdhury ◽

Shahin Ara Begum ◽

...

Keyword(s):

Pathogenic Bacteria ◽

Age Groups ◽

Medical College Hospital ◽

Diffusion Method ◽

Aeromonas Species ◽

Biochemical Tests ◽

College Hospital ◽

E Coli ◽

Medical College ◽

Almost All

Diarrhoea is a self-limiting disease caused by various enteropathogens and antimicrobial therapy is an important adjunct to fluid therapy in the management of the cases. We have studied the flood-affected diarrhoeal (post-flood diarrhoea) patients admitted in Dhaka Medical College Hospital during August to September, 2007. A total of ll4 faecal samples were collected from the patients of all age groups, and investigated for bacterial enteropathogens. Specimens were cultured in appropriate media and identification of the organisms showing growth was done by relevant biochemical tests. In addition, the isolates of Vbrio cholera wer confirmed using pollvalent antisera. Sensitivity of the isolated organisms was done by disc diffusion method. Among the isolated bacterial enteropathogens, majority was V. cholera (46, 40.3%). Other isolates included Escherichia coli (18, 23.0%), Aeromonas species (8,10.O%) and V parahaemolyticus (5, 7.0%). No pathogenic bacteria were isolated from 27 of the cases. No Salmonella or Shigella was detected in any case. Majority of the cases (24, 52.0%) infected with V cholerae were children having less than L0 years of age. Most of the isolates of V cholerae (77.7%) were sensitive to Ciprofloxacin. Almost all of the V cholerae isolates (97.90 ) were resistant to Tetracycline, followed by Co-trimoxazloe (93,5%) and Nalidixic acid (59.2%). All of the E. coli isolates (100.0%) were sensitive to Ceftriaxone, followed by Ceftazidime (88.8%) and Ciprofloxacin (77.7oh). Thus it can be concluded that Ciprofloxacin and Ceftriaxone can be considered as the drugs ofchoice for the treatment of post-flood diarrhoea along with proper rehydration therapy.Bangladesh J Med Microbiol2008; 02 (01):13-17DOI: http://dx.doi.org/10.3329/bjmm.v8i1.21929

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The Microbiology of Serous and Mucoid Otitis Media

PEDIATRICS ◽

10.1542/peds.63.6.915 ◽

1979 ◽

Vol 63 (6) ◽

pp. 915-919

Author(s):

G. Scott Giebink ◽

Elaine L. Mills ◽

John S. Huff ◽

Charlene K. Edelman ◽

Marcia L. Weber ◽

...

Keyword(s):

Haemophilus Influenzae ◽

Otitis Media ◽

Mycoplasma Pneumoniae ◽

Staphylococcus Epidermidis ◽

Anaerobic Bacteria ◽

Positive Culture ◽

Aerobic Bacteria ◽

Viral Isolate ◽

Aerobic And Anaerobic ◽

Culture Positive

One hundred forty-four serous and mucoid effusions were cultured for aerobic and anaerobic bacteria, Mycoplasma pneumoniae, and virus. Thirty percent of all effusions yielded an unequivocally positive culture for aerobic bacteria. Although serous effusions were culture positive as often as mucoid effusions, Haemophilus influenzae was isolated predominantly from serous effusions and Staphylococcus epidermidis predominantly from mucoid samples. Only one of 73 effusions yielded a viral isolate (Herpesvirus honiinis). None of 33 effusions yielded M pneumoniae, and only one of 17 effusions yielded an anaerobe (Propionibacterium). These findings suggest that aerobic bacteria may play a role in the pathogensis of serous and mucoid otitis media.

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Pattern of Carbapenem Resistance in Enterobacteriaceae in Rajshahi Medical College Hospital

TAJ Journal of Teachers Association ◽

10.3329/taj.v33i2.51341 ◽

2020 ◽

Vol 33 (2) ◽

pp. 63-68

Author(s):

Ayesha Siddica ◽

Md Bulbul Hasan ◽

Md Shah Alam ◽

Sabera Gul Nahar ◽

Nurjahan Begum ◽

...

Keyword(s):

Carbapenem Resistance ◽

Medical College Hospital ◽

Biochemical Tests ◽

College Hospital ◽

E Coli ◽

Medical College ◽

Gram Staining ◽

Carbapenem Resistant ◽

Colonial Morphology ◽

Carbapenem Resistant Enterobacteriaceae

The global emergence and spread of Carbapenem Resistant Enterobacteriaceae have been threatening the ability to treat an infection. Hence the present study was carried out with the aim to isolate important members of Enterobacteriaceae family with identification of carbapenem resistant isolates among them. The study was done in the Department of Microbiology, Rajshahi Medical College with collaboration of different disciplines of RMCH from January 2019 to December 2019. Samples were collected purposively. Causative organisms were isolated by culture and identified by colonial morphology, gram staining and relevant biochemical tests. Identified Enterobacteriaceae those showed resistance to carbapenem (imipenem, meropenem) were tested phenotypically by Modified Hodge Test (MHT) to see carbapenemase production. A total of 97 Enterobacteriaceae were isolated from 275 samples. E. coli (54.64%) was the most frequent isolate. By Modified Hodge Test, 19(19.59%) bacteria were phenotypically confirmed as Carbapenem Resistant Enterobacteriaceae (CRE). This study signifies that carbapenem resistance is increasing at an alarming rate. TAJ 2020; 33(2): 63-68

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Isolation of post operative hospital acquired infections in a private hospital of Bangladesh and identification of clonal relatedness of E. coli by using Pulse filed gel electrophoresis

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v6i2.19369 ◽

2012 ◽

Vol 6 (2) ◽

pp. 7-10

Author(s):

Mohammad Murshed ◽

Sabeena Shahnaz ◽

Md. Abdul Malek

Keyword(s):

Private Hospital ◽

Isolation And Identification ◽

College Hospital ◽

Hospital Acquired Infection ◽

E Coli ◽

Hospital Acquired Infections ◽

Medical College ◽

Clonal Relatedness ◽

Hospital Acquired ◽

Dressing Materials

Isolation and identification of post operative hospital acquired infection was carried out from July 2008 to December 2008 in Holy Family Red Crescent Medical College Hospital (private hospital). The major pathogen of wound infection was E. coli. A total; of 120 samples were collected from the surrounding environment of post operative room like floor, bed sheets, instruments, dressing materials, catheter, nasogastric and endotracheal tube. E. coli (40%) was the predominant organism followed by S. aureus (24%). DNA fingerprinting analysis using pulsed field gel electreopheresis of XbaI restriction digested genomic DNA showed that clonal relatedness between the two clinical nd environmental isolates were 100%.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19369 Bangladesh J Med Microbiol 2012; 06(02): 7-10

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In Vitro and In Vivo Antibacterial Activities of a Novel Glycylcycline, the 9-t-Butylglycylamido Derivative of Minocycline (GAR-936)

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.43.4.738 ◽

1999 ◽

Vol 43 (4) ◽

pp. 738-744 ◽

Cited By ~ 283

Author(s):

P. J. Petersen ◽

N. V. Jacobus ◽

W. J. Weiss ◽

P. E. Sum ◽

R. T. Testa

Keyword(s):

Anaerobic Bacteria ◽

Tetracycline Resistance ◽

Protective Effects ◽

Gram Negative ◽

E Coli ◽

Resistance Determinants ◽

Aerobic And Anaerobic ◽

Ribosomal Protection

ABSTRACT The 9-t-butylglycylamido derivative of minocycline (TBG-MINO) is a recently synthesized member of a novel group of antibiotics, the glycylcyclines. This new derivative, like the first glycylcyclines, theN,N-dimethylglycylamido derivative of minocycline and 6-demethyl-6-deoxytetracycline, possesses activity against bacterial isolates containing the two major determinants responsible for tetracycline resistance: ribosomal protection and active efflux. The in vitro activities of TBG-MINO and the comparative agents were evaluated against strains with characterized tetracycline resistance as well as a spectrum of recent clinical aerobic and anaerobic gram-positive and gram-negative bacteria. TBG-MINO, with an MIC range of 0.25 to 0.5 μg/ml, showed good activity against strains expressing tet(M) (ribosomal protection), tet(A), tet(B),tet(C), tet(D), and tet(K) (efflux resistance determinants). TBG-MINO exhibited similar activity against methicillin-resistant Staphylococcus aureus (MRSA), penicillin-resistant streptococci, and vancomycin-resistant enterococci (MICs at which 90% of strains are inhibited, ≤0.5 μg/ml). TBG-MINO exhibited activity against a wide diversity of gram-negative aerobic and anaerobic bacteria, most of which were less susceptible to tetracycline and minocycline. The in vivo protective effects of TBG-MINO were examined against acute lethal infections in mice caused by Escherichia coli, S. aureus, andStreptococcus pneumoniae isolates. TBG-MINO, administered intravenously, demonstrated efficacy against infections caused byS. aureus including MRSA strains and strains containingtet(K) or tet(M) resistance determinants (median effective doses [ED50s], 0.79 to 2.3 mg/kg of body weight). TBG-MINO demonstrated efficacy against infections caused by tetracycline-sensitive E. coli strains as well asE. coli strains containing either tet(M) or the efflux determinant tet(A), tet(B), ortet(C) (ED50s, 1.5 to 3.5 mg/kg). Overall, TBG-MINO shows antibacterial activity against a wide spectrum of gram-positive and gram-negative aerobic and anaerobic bacteria including strains resistant to other chemotherapeutic agents. The in vivo protective effects, especially against infections caused by resistant bacteria, corresponded with the in vitro activity of TBG-MINO.

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Taking Screenshots of the Invisible: A Study on Bacterial Contamination of Mobile Phones from University Students of Healthcare Professions in Rome, Italy

Microorganisms ◽

10.3390/microorganisms8071075 ◽

2020 ◽

Vol 8 (7) ◽

pp. 1075 ◽

Cited By ~ 1

Author(s):

Domenico Cicciarella Modica ◽

Massimo Maurici ◽

Gian Loreto D’Alò ◽

Cinzia Mozzetti ◽

Alessandra Messina ◽

...

Keyword(s):

Mobile Phones ◽

Staphylococcus Epidermidis ◽

Univariate Analysis ◽

Clinical Settings ◽

Linear Regression Models ◽

Biochemical Tests ◽

E Coli ◽

Plate Counts ◽

Healthcare Professions ◽

Heterotrophic Plate Counts

Mobile phones (MPs) are commonly used both in the personal and professional life. We assessed microbiological contamination of MPs from 108 students in healthcare professions (HPs), in relation to their demographic characteristics and MPs handling habits, collected by means of a questionnaire. Cultural and biochemical tests were performed, and statistical analyses were carried out. Staphylococci were present in 85% of MPs, Enterococci in 37%, Coliforms in 6.5%; E. coli was never detected. Staphylococcus epidermidis was the most frequently isolated staphylococcal species (72% of MPs), followed by S. capitis (14%), S. saprophyticus, S. warneri, S. xylosus (6%), and by S. aureus (4%). Heterotrophic Plate Counts (HPC) at 37 °C, ranged from 0 to 1.2 × 104 CFU/dm2 (mean = 362 CFU/dm2). In univariate analysis, the male gender only was significantly associated with higher HPCs and enterococcal contamination. Multiple linear regression models explained only 17% and 16% of the HPC 37 °C and staphylococcal load variability, respectively. Developing specific guidelines for a hygienic use of MPs in clinical settings, for preventing cross-infection risks, is advisable, as well as introducing specific training programs to HP students. MPs decontamination procedures could also be implemented in the community.

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Microbiological Study of Chronic Suppurative Otitis Media

Bangladesh Medical Journal ◽

10.3329/bmj.v46i2.40210 ◽

2019 ◽

Vol 46 (2) ◽

pp. 7-9

Author(s):

Rifat Nousin Shumi ◽

Abdullah Siddiqe ◽

Arefa Akter

Keyword(s):

Otitis Media ◽

Age Groups ◽

Chronic Suppurative Otitis Media ◽

Klebsiella Pneumonia ◽

Biochemical Tests ◽

College Hospital ◽

Suppurative Otitis Media ◽

Medical College ◽

Microbiological Profile ◽

Gram Staining

Chronic suppurative otitis media (CSOM) is a prevailing and notorious infection in developing countries causing serious local damage and threatening complications. The purpose of the present study was determine the microbiological profile of isolates from discharge in CSOM. This study was conducted at out-patient department of ENT at Rajshahi Medical College Hospital, Rajshahi from January 2014 to December 2014. Samples were taken from 185 patients (both male and female) in all age groups suffering from chronic suppurative otitis media. Their Gram staining, culture, and biochemical tests were carried out to identify the organisms. It showed the predominance by staphylococcus aereus (29.13%), followed by Pseudomonas Aeruginosa (22.83%), Streptococcus Pyogen (14.96%), E.Coli (9.44%), Proteus Mirabilis (6.29%), Klebsiella Pneumonia ( 4.72%). Bangladesh Med J. 2017 May; 46 (2): 7-9

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Bacteriology of Chronic Maxillary Sinusitis in Adults

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348948909800605 ◽

1989 ◽

Vol 98 (6) ◽

pp. 426-428 ◽

Cited By ~ 99

Author(s):

Itzhak Brook

Keyword(s):

Staphylococcus Aureus ◽

Chronic Sinusitis ◽

Maxillary Sinusitis ◽

Anaerobic Bacteria ◽

Chronic Maxillary Sinusitis ◽

Aerobic And Anaerobic ◽

And Staphylococcus Aureus ◽

Culture Positive ◽

Maxillary Sinuses

Aspirates of 72 chronically inflamed maxillary sinuses were processed for aerobic and anaerobic bacteria. Bacterial growth was present in 66 of the 72 specimens (92%). Anaerobic bacteria were isolated in 58 of the 66 culture-positive specimens (88%). Anaerobes only were recovered in 37 cases (56%) and in 21 (32%) they were mixed with aerobic or facultative bacteria. Aerobic or facultative bacteria were present in eight cases (12%). A total of 185 isolates (2.8 per specimen) — 131 (2.0 per specimen) anaerobes and 54 (0.8 per specimen) aerobes or facultatives — were isolated. The predominant anaerobic organisms were anaerobic cocci and Bacteroides sp, and the predominant aerobes or facultatives were Streptococcus sp and Staphylococcus aureus. Twelve of the 27 Bacteroides sp that were tested for β-lactamase (44%) produced the enzyme. These findings indicate the major role of anaerobic organisms in chronic sinusitis.

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Aerobic and Anaerobic Microbiology of Surgical-Site Infection Following Spinal Fusion

Journal of Clinical Microbiology ◽

10.1128/jcm.37.3.841-843.1999 ◽

1999 ◽

Vol 37 (3) ◽

pp. 841-843 ◽

Cited By ~ 7

Author(s):

Itzhak Brook ◽

Edith H. Frazier

Keyword(s):

Escherichia Coli ◽

Spinal Fusion ◽

Bacterial Growth ◽

Anaerobic Bacteria ◽

Surgical Site Infections ◽

Aerobic Bacteria ◽

Site Infection ◽

E Coli ◽

Naval Hospital ◽

Aerobic And Anaerobic

The aerobic and anaerobic microbiology of surgical-site infections (SSI) following spinal fusion was retrospectively studied. This was done by reviewing the clinical and microbiological records at the Naval Hospital in Bethesda, Md., from 1980 to 1992. Aspirates of pus from 25 infection sites showed bacterial growth. Aerobic bacteria only were recovered from 9 (36%) specimens, anaerobic bacteria only were recovered from 4 (16%), and mixed aerobic and anaerobic bacteria were recovered from 12 (48%). Sixty isolates were recovered: 38 aerobes (1.5 isolates per specimen) and 22 anaerobes (0.9 isolate per specimen). The predominant aerobes were Escherichia coli(n = 8) and Proteus sp. (n = 7). The predominant anaerobes wereBacteroides fragilis group (n = 9) andPeptostreptococcus sp. (n = 6) isolates. An increase in recovery of E. coli and B. fragilis was noted in patients with bowel or bladder incontinence. This study highlights the polymicrobial nature of SSI and the importance of anaerobic bacteria in SSI following spinal fusion.

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