scholarly journals Preliminary Phytochemical Screenings and Pharmacological Activities of Three Medicinal Plants of Bangladesh

2018 ◽  
Vol 16 (2) ◽  
pp. 195-203
Author(s):  
Mohammed Ibrahim ◽  
Md Ruhul Kuddus ◽  
Md Aslam Hossain ◽  
Muhammad Abdullah Al Mansur ◽  
Mohammad A Rashid
Keyword(s):  
Medicinal Plants ◽  
Methanol Extract ◽  
Protein Denaturation ◽  
Anxiolytic Effect ◽  
Salmonella Typhi ◽  
Pharmacological Activities ◽  
Antipyretic Effect ◽  
Anti Inflammatory ◽  
Cross Test

The present study was designed to evaluate the preliminary phytochemical screening and antimicrobial, antidiarrheal, anti-inflammatory, analgesic, antipyretic, anxiolytic, thrombolytic and membrane stabilizing properties of the methanolic extract of three medicinal plants Perilla ocymoides L., Murraya koenigii (Linn.) Spreng., Baliospermum montanum (Wild.) Muell growing in Bangladesh. In antimicrobial test, maximum zone of inhibition was found against Salmonella typhi (18.0 mm) and Escherichia coli (17.0 mm) by B. montanum extract. In the castor oil-induced antidiarrheal assay, the methanol extract of M. koenigii showed maximum 50% inhibition of defecation. During in-vitro anti-inflammatory test, the methanol extract of B. montanum at 500 μg/ml, b.w. revealed 39.62% inhibition of protein denaturation. Due to analgesia, the M. koenigii extract showed 53.29% inhibition of acetic acidinduced writhing reflex in experimental mice. Antipyretic effect of P. ocymoides, M. Koenigii and B. montanum extractives was assessed by Brewer’s yeast-induced pyrexia in mice. The B. montanum extract possesses significant anxiolytic effect that was evidenced by both hole cross test and open field test in mice. In thrombolytic assay, the highest activity (57.81%) was observed by B. montanum extract. Results of the preliminary phytochemical screenings demonstrated the presence of alkaloids, glycosides, flavonoids, reducing sugars, gums etc.Dhaka Univ. J. Pharm. Sci. 16(2): 195-203, 2017 (December)

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF NIEBUHRIA APETALA (ROTH) DUNN – IN VITRO MODELS

2019 ◽  
pp. 278-281
Author(s):  
RAJESH A ◽  
DOSS A ◽  
TRESINA PS ◽  
MOHAN VR
Keyword(s):  
Methanol Extract ◽  
Protein Denaturation ◽  
In Vitro Models ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Inflammatory Agent ◽  
Potential Source ◽  
Anti Inflammatory

Objective: The objective of this study was to determine the anti-inflammatory activity of methanol extract of Niebuhria apetala and its possible mechanism of action. Methods: Methanol extract of Niebuhria apetala leaf (NAL) was assessed for its anti-inflammatory activity by in vitro methods. Using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and antilipoxygenase activity at different concentrations, in vitro anti-inflammatory activity was estimated. The standard drug used for this purpose was aspirin. Results: Methanol extract NAL at a concentration range of 100–500 μg/ml significant (p<0.01) protects the heat-induced protein denaturation. At the concentration of 500 mg/ml, NAL showed significant (p<0.01) inhibition of protease inhibitory action. Heat-induced hemolysis of erythrocyte, hypotonicity-induced hemolysis, and lipooxygenase activity were significant (p<0.01) inhibited at the concentration of 500 μg/ml. Conclusion: Finally, the present study indicates that methanol extract of Niebuhria apetala can be a potential source of anti-inflammatory agent.

scholarly journals In vitro antioxidant, anti-inflammatory and anti-cancer activities of methanolic extract of Asparagus horridus grows in North Cyprus Kuzey Kıbrıs da yetişen Asparagus horridus metanolik ekstraktının in-vitro antioksidan, anti-enflamatuar ve anti-kanser aktivitesi

2020 ◽  
Vol 45 (4) ◽  
pp. 365-372
Author(s):  
Ergul Mutlu Altundag ◽  
Duygu Gençalp ◽  
Cahit Özbilenler ◽  
Kübra Toprak ◽  
Namık Kerküklü
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Protein Denaturation ◽  
Soxhlet Extraction ◽  
Edible Plant ◽  
North Cyprus ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Inflammatory Effect

AbstractBackgroundAsparagus horridus is an edible plant known as “Ayrelli” in North Cyprus. The scientific literature has not yet submitted a report about the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus plant from North Cyprus until now. The purpose of the research was to determine the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus.Materials and methodsSoxhlet extraction of A. horridus was performed using methanol. Antioxidant activity was determined by DPPH, TFC, FRAP and TPC assays. Protein-denaturation assay was performed to determine the anti-inflammatory effect. The anti-cancer effects of the extract on HepG2 and B-CPAP cell lines were determined with MTT assay.ResultsAntioxidant activity for A. horridus extract was determined by DPPH (50%), TFC (266.26 μg QUE/mg extract), FRAP (1.27 μg FeSO4/mg extract) and TPC (167.613 μg GAE/mg extract) assays at 25 mg/mL. Inhibition of protein-denaturation activity was found as 29.42% at 25 mg/mL. After 24 h of the extract treatment, cell proliferation of HepG2 and B-CPAP cancer cells were inhibited at IC50 values 63.24 μg/mL and 101.24 μg/mL, respectively.ConclusionThese results have shown that the methanol extract of A. horridus grows in North Cyprus has antioxidant, anti-inflammatory and anti-cancer activities.

scholarly journals In vitro antioxidant and anti-inflammatory activities of methanol extract of Vitellaria paradoxa seed (shea seed)

2021 ◽  
Vol 11 (3) ◽  
pp. 057-065
Author(s):  
Babafemi Tosin Ogunbiyi ◽  
Oluwaseyi Adegoke Adetunji ◽  
Olubunmi Esther Ogunbiyi ◽  
Gogonte Hezekiah Amah ◽  
Modupe Olusola Adetayo ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Protein Denaturation ◽  
Antioxidant Activities ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Standard Drug ◽  
Maximum Percentage ◽  
Total Antioxidant ◽  
Anti Inflammatory

Vitellaria paraodoxa (shea tree) is the source of shea seed from which the well-known shea butter is derived. The methanol extract of shea seed was evaluated for its anti-inflammatory and antioxidant activities using diclofenac sodium and ascorbic acid as standard respectively in in vitro methods. The anti-inflammatory activity was determined by inhibition of protein denaturation of bovine serum albumin (BSA) and erythrocyte membrane stabilization of human red blood cell. The antioxidant activity was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) assays. The results showed that methanol extract of V. paradoxa seed at different concentration protects the heat induced protein denaturation with the maximum percentage inhibition of 27% (IC50=303.0 µg/mL, p<0.05) at 500µg/mL compared to the standard drug which induced maximum inhibition of 45% (IC50=261.4 µg/mL, p<0.05) at 500 µg/mL and the control. The percentage inhibition of the methanol extract and standard drug in erythrocyte stabilization assay increased in a concentration dependent manner with maximum inhibitory activity of 96.9% (IC50=31.47 µg/mL, p<0.05) and 95% (IC50=33.89 µg/mL, p<0.05) at 2000 µg/ml respectively, which indicates that methanol extract stabilized erythrocyte membrane against hypotonic induced hemolysis in a blood sample better than the standard drug. The maximum percentage inhibition of methanol extract and standard drug in DPPH assay were found to be at 97% (IC50=8.95 µg/mL, p<0.05) and 98% (IC50=6.72 µg/mL, p<0.05) respectively at 100 µg/ml. The absorbance of the reductive capacities in FRAP assay indicates that the methanol extract has higher reducing potency in a concentration dependent manner. The methanol extract exhibited total antioxidant capacity of 0.25 ± 0.04 µg/(AAE/g) when compared to the standard drug 0.87 ± 0.03 µg/(AAE/g) at highest concentration of 100 µg/ml. For TBARS assay, low absorbance value indicate a high level of inhibition of lipid peroxidation. The maximum percentage inhibition of methanol extract was 97.5 % (IC50=51.79 µg/mL, p<0.05) and ascorbic acid was 99% (IC50=52.30 µg/mL, p<0.05) at concentration of 20 µg/ml. The assay indicates that the methanol extract has higher inhibiting potency in a reverse concentration dependent manner. In conclusion, V. paradoxa seed may possess strong anti-inflammatory and antioxidant activities which could constitute a potential source for development of new therapy.

Anti-inflammatory, anti-diarrheal, thrombolytic and cytotoxic activities of an ornamental medicinal plant: Persicaria orientalis

2017 ◽  
Vol 28 (1) ◽  
Author(s):  
Prawej Ansari ◽  
Md. Josim Uddin ◽  
Md. Masudur Rahman ◽  
Md. Abdullah-Al-Mamun ◽  
Md. Rabiul Islam ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Castor Oil ◽  
Methanol Extract ◽  
Protein Denaturation ◽  
Clot Lysis ◽  
Cytotoxic Activities ◽  
Hypotonic Medium ◽  
Inflammatory Models ◽  
Anti Inflammatory

AbstractBackground:, an ornamental medicinal plant, has been used in traditional medicine for the treatment of various diseases. Although the plant is reported to have some important pharmacological effects, many medicinal values remain unidentified. Our objective was to evaluate the anti-inflammatory, anti-diarrheal, thrombolytic, and cytotoxic properties of the methanol extract ofMethods:Anti-inflammatory activity was measured by the inhibition of hypotonicity-induced human red blood cell hemolysis and albumin denaturation technique in vitro of Po-MeOH. Diarrheal episodes were examined in mice with castor oil-induced diarrhea. The clot lysis and brine shrimp lethality bioassay in vitro were used to evaluate the thrombolytic and cytotoxic activities of the plant extract, respectively.Results:Using in vitro anti-inflammatory models, the results demonstrated that Po-MeOH at the five different dose ranges from 31.25 to 500 μg/mL significantly (p<0.05) protected (0.98%–50.71%) the erythrocyte membrane against lysis induced by hypotonic medium solution and protein denaturation (38.27%–79.22%) of bovine albumin, respectively. The extract exhibited a significant reduction of severity (75.17%) of castor oil-induced diarrhea in mice at the highest dose of 400 mg/kg compared to loperamide (82.06%) at 5 mg/kg. Po-MeOH also showed 33.14% clot lytic activity in the thrombolytic test and cytotoxicity with LCConclusions:These findings suggest that Po-MeOH has significant anti-inflammatory and anti-diarrheal effects along with moderate thrombolytic and lower cytotoxic properties that may warrant the further exploration.

scholarly journals Preliminary Phytochemical Analysis: In-Vitro Comparative Evaluation of Anti-arthritic and Anti-inflammatory Potential of Some Traditionally Used Medicinal Plants

Dose-Response ◽  
2022 ◽  
Vol 20 (1) ◽  
pp. 155932582110697
Author(s):  
Sundas Farooq ◽  
Ghazala Shaheen ◽  
Hafiz Muhammad Asif ◽  
Muhammad Rahil Aslam ◽  
Rabia Zahid ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Protein Denaturation ◽  
Aloe Vera ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Aloe Barbadensis ◽  
Standard Drug ◽  
Colchicum Autumnale ◽  
Anti Inflammatory

Background: Colchicum autumnale, Strychnous nux-vomica and Aloe barbadensis are the medicinal plants clinically utilized for the management of rhuematic disorders. Purpose: The present work was focused to evaluate the in-vitro anti-arthritic and anti-inflammatory activities of Colchicum ( Colchicum autumnale), Nux-vomica ( Strychnous nux-vomica), and Aloe-vera ( Aloe barbadensis). Research Design: Primarily, the aqueous-ethanolic extracts of these medicinal plants were phytochemically screened followed by Fourier Transform Infrared (FTIR) analysis. Anti-arthritic activity by protein denaturation method and anti-inflammatory activity by human red blood cell (HRBC) membrane stabilization method at the concentration of 125, 250, and 500 µg/mL along with standard were performed. Results: Phytochemical screening revealed that alkaloids, saponins, terpenoids, phenols, and anthraquinones were found in all the extracts, and organic acids, amine group, aromatic or aliphatic compounds, esters and halogens, and phenolics were identified by FTIR. Protein denaturation method revealed that colchicum, nux-vomica, and aloe-vera showed maximum 98.5%, 99.6%, and 72.3% of inhibition at 500 µg/mL compared with that of standard drug, that is, Diclofenac sodium. Membrane stabilization method showed that colchicum, nux-vomica, and aloe-vera showed maximum 40.20%, 35.67%, and 40.1% protection at 500 µg/mL when compared with standard drug. Conclusion: It is concluded from the current study that extracts of colchicum, nux-vomica, and aloe-vera showed more potent effect and thus can be used as alternative options for the management of inflammatory and arthritic ailments.

scholarly journals Potential Investigation of In Vitro Antioxidant, Anti-Inflammatory and Anti-Haemolytic Activities from Polar Solvent Extracts of Pterocarpus marsupium

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Ramesh L. Londonkar ◽  
Aruna L. H. ◽  
Amarvani P. Kanjikar
Keyword(s):  
Methanol Extract ◽  
Protein Denaturation ◽  
Biological Activities ◽  
Reducing Power ◽  
Total Phenolic ◽  
Pterocarpus Marsupium ◽  
Anti Inflammatory ◽  
Phenolic And Flavonoid ◽  
In Vitro Antioxidant Activity

Pterocarpus marsupium is a well known plant in ayurvedic system of medicine. The plant has been found to possess diverse number of biological activities and is thus commercially exploited. In the present study, methanol and aqueous extracts of P.marsupium bark were used to evaluate in vitro antioxidant, anti-inflammatory and anti-haemolytic activities. In vitro antioxidant activity was carried out by DPPH, ABTS, phosphomolybdenum, reducing power assays, anti- inflammatory activity by inhibition of protein denaturation and anti-haemolytic by hyposaline induced haemolysis. Total phenolic and flavonoid content was also estimated. Methanol extract showed an effective pharmacological activity in all assays when compared with their respective standards. The maximum phenolic and flavonoid contents were found to be (280 ±0.47mg/g) and (620±0.81mg/g) respectively in methanol extract of Pterocarpus marsupium. This study indicates the presence of active constituents which can be exploited for the treatment of various diseases and also could be used in pharmaceutical industry.

Curcumin: Natural Antimicrobial and Anti Inflammatory Agent

2021 ◽  
pp. 1-8
Author(s):  
Pehlivanović Belma ◽  
Čaklovica Kenan ◽  
Lagumdžija Dina ◽  
Omerović Naida ◽  
Žiga Smajić Nermina ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Protein Denaturation ◽  
Dose Range ◽  
In Vitro Assays ◽  
Natural Antimicrobial ◽  
In Vivo Models ◽  
Vitro Assay ◽  
Inflammatory Agent ◽  
Anti Inflammatory

The pursuance of novel antimicrobial and anti-inflammatory agents has been expanding due to a significant need for more efficient pharmacotherapy of various infections and chronic diseases. During the last decade, pharmacokinetics, pharmacodynamics and pharmacological properties of curcumin have been extensively studied. The aim of the present study was to evaluate the antibacterial activity of curcumin against both Gram-positive and Gram-negative bacteria as well as its antifungal activity by using in vitro agar well diffusion assay. Moreover, the anti-inflammatory activity of curcumin was determined with in vitro assay of inhibition of protein denaturation. Results demonstrated wide antimicrobial activity of curcumin upon all of the test bacteria and fungi. The strongest activity of curcumin was observed at a concentration of 0.50 mg/ml against S. aureus, L. monocytogenes, E. coli, P. aeruginosa and C. albicans, resulting in a maximum zone of inhibition of 14.7 mm, 14.3 mm, 13.7 mm, 10.7 mm and 10.7 mm, respectively. Findings suggested that the antimicrobial activity of curcuminis dependent upon the concentrations. Furthermore, results demonstrated high effectiveness of curcumin compared to standard acetylsalicylic acid in inhibiting heat-induced protein denaturation, which activity is also depended upon the concentrations. The present study emphasises the potential application of curcumin as a natural antimicrobial and anti-inflammatory agent. However, findings of this study are restricted to in vitro assays and consideration should be given to conducting a study involving wider dose range test substances as well as including further research on in vivo models.

scholarly journals Preliminary phytochemical screenings and Pharmacological activities of Blumea lacera (Burn.f.) DC.

2015 ◽  
Vol 13 (1) ◽  
pp. 69-73
Author(s):  
Md Abul Khair ◽  
Mohammed Ibrahim ◽  
Qamrul Ahsan ◽  
Md Ruhul Kuddus ◽  
Ridwan Bin Rashid ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Crude Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Acetyl Salicylic Acid ◽  
Antipyretic Activity ◽  
Whole Plant ◽  
Anti Inflammatory ◽  
Blumea Lacera

The methanol extract of the whole plant of Blumea lacera (Burn.f.) DC. (BLME) has been subjected to preliminary screenings for phytoconstituents and antipyretic, analgesic and anti-inflammatory activities. Antipyretic activity was assessed by the yeast-induced hyperthermia in mice. The analgesic property was evaluated by formalin-induced writhing test. Acetyl salicylic acid (ASA) was used as standard for in-vitro anti-inflammatory activity test. In yeast-induced pyrexia, the crude extract demonstrated a significant (p=0.05) reduction in body temperature of mice after elevation by the administration of yeast. These effects were pronounced at the 2nd and 3rd h of post-treatment with the extract. BLME exhibited a dose-dependent analgesic activity with 39.13% and 56.52% protection at 200-and 400-mg/kg, b.w., respectively as compared to 76.09% revealed by the standard diclofenac sodium. In the anti-inflammatory test, the crude extract at 400 ?g/ml displayed 62.40% inhibition of protein denaturation whereas standard acetyl salicylic acid exhibited 76.74% inhibition. Results of the preliminary phytochemical screenings demonstrated the presence of alkaloids, flavonoids and triterpenoids in the extract. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21863 Dhaka Univ. J. Pharm. Sci. 13(1): 69-73, 2014 (June)

scholarly journals Anti-Inflammatory Effects of 3-Formyl, 7-Flavonols Derivatives by Microwave Enhanced Chemistry Assisted - Vilsmeier Haack Synthesis

2019 ◽  
Vol 12 (04) ◽  
pp. 1779-1791
Author(s):  
M. Karpakavalli ◽  
A.Y. Sangilimuthu ◽  
A.Usha Raja Nanthini ◽  
G.Nagaraja Perumal ◽  
S. Mohan ◽  
...  
Keyword(s):  
Protein Denaturation ◽  
Population Increase ◽  
Spectral Studies ◽  
Dimethyl Formamide ◽  
Mass Spectral ◽  
Paw Oedema ◽  
Rf Values ◽  
Anti Inflammatory

In the modern medicines the novel and active molecules are essential to act against various diseases and increase the needs day by day due to population increase. In view of that, we attempt to make a variety of synthetic molecules against inflammation by a new and popular greener microwave assisted and faster method such as Microwave Enhanced Chemistry assisted Vilsmeier Haack Synthesis (MEC-VHS). In this paper, we report the synthesis of nitro- dinitro- and acetyl- derivatives of 3- formyl, 7-flavonols using MEC-VHS techniques against inflammation as anti-inflammatory agent. These derivatives were synthesized via pinkish formylation complex of dimethyl formamide and phosphorous oxychloride by microwave irradiation resulted as suspension by base. The re-crystallized products were characterized through Co-TLC, λmax, IR, HPTLC, 1HNMR, CHN analysis and mass spectral studies. The HPTLC finger print profiles obtained were of with a prominent single peak and with a matching Rf values compared to that obtained by an ordinary Co-TLC technique. All the synthesized compounds were screened for their anti-inflammatory activity by in vitro protein denaturation method and in vivo carrageenan induced paw oedema method and it was found that all the compounds excepting the un-substituted 3-formyl, 7-flavonols gave an equi- or more potent activity as compared to that of the standard.

scholarly journals Synthesis, Characterization and Evaluation of Anti-inflammatory Activity of Novel Indoline Derivatives

2016 ◽  
Vol 12 (12) ◽  
pp. 4557-4563
Author(s):  
Dhinesh kumar Manoharan
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Potent Inhibition ◽  
Ft Ir ◽  
Anti Inflammatory

Series of indoline derivatives were synthesized using N-(4-aminophenyl) indoline-1-carbothiamide as a precursor. The structures of synthesized compounds were confirmed by   FT-IR, 1H-NMR, 13C-NMR and LC-MS. The in vitro anti-inflammatory activity of synthesized indoline derivatives were examined by standard anti-denaturation assay. The compounds 4a (IC50 = 62.2 µg/ml) and 4b (IC50 = 60.7 µg/ml) showed potent inhibition on protein denaturation. The compounds 5a (IC50 = 97.8 µg/ml) exhibits moderate inhibition on protein denaturation

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