scholarly journals Phytochemical and pharmacological evaluations of Polygonum lapathifolium stem extract for anthelmintic and antiemetic activity

2013 ◽  
Vol 2 (3) ◽  
pp. 57-62
Author(s):  
Latifa Bulbul ◽  
Somen Mojumder Sushanta ◽  
Md. Jahir Uddin ◽  
Shahnaj Tanni
Keyword(s):  
Anthelmintic Activity ◽  
Pharmaceutical Journal ◽  
Antiemetic Effect ◽  
Standard Drug ◽  
Reference Drug ◽  
Phytochemical Investigation ◽  
Antiemetic Activity ◽  
Stem Extract ◽  
Polygonum Lapathifolium

The present study is an attempt to preliminary phytochemical investigation, anthelmintic and anti-emetic activity studies on methanolic extract of Polygonum lapathifolium stems. The phytochemical screening shows the presence of phytosterols, diterpens, amino acid & protein, alkaloid and flavonoids, those are responsible for antiviral, antibacterial, antiallergic, antihypertensive, antiarrythmic, hepatoprotective, anti-inflammatory effects in mammals. In Anthelmintic activity test (using Pheretima posthuma model) five concentrations (20, 40, 60, 80 and 100 mg/ml in distilled water) of extracts were used which involved the determination of time of paralysis (vermifuge) and time of death (vermicidal activity) of the worms, where the concentration of 60, 80 &100 mg/ml showed a significant anthelmintic activity. Observations were comparable with the standard drug at concentration of 10mg/ml of piperizine citrate. On the other hand, in anti-emetic test, emesis was induced by the oral administration of copper sulphate 50mg/kg body weight to four days age of young chicks using chick emesis model. The antiemetic activity was determined by calculating the mean decrease in number of retching in contrast with those of control disorders. The extracts (150 mg /kg orally) showed statistically significant antiemetic effect (90.45% Inhibition) compared with reference drug metoclopramide (50mg/kg intraperitoneally) which showed 82.48% Inhibition. From these observations, it was concluded that stems extracts have potential anthelmintic and anti-emetic properties. The plant may further be explored for its various pharmacological activities.DOI: http://dx.doi.org/10.3329/icpj.v2i3.13582 International Current Pharmaceutical Journal, February 2013, 2(3): 57-62 

scholarly journals Phytochemicals, Nutritional Value, Antioxidant, and Anticoagulant Activity of Lactuca sativa L. Leaves and Stems

2020 ◽  
Vol 3 (3) ◽  
pp. 152-161
Author(s):  
Hanin Nafed Mughrbi ◽  
Abdurazag Abdussalam Auzi ◽  
Hamza Maghrbi
Keyword(s):  
Antioxidant Activity ◽  
Lactuca Sativa ◽  
Anticoagulant Activity ◽  
Nutrient Content ◽  
Dpph Assay ◽  
Significant Prolongation ◽  
Phytochemical Investigation ◽  
Stem Extract

The main ingredient in the daily salad dish is the leaves of Lactuca sativa, while the stems are usually discarded and not eaten. This study was conducted to compare the stems and leaves of two selected L. sativa varieties (L. sativa var. longifolia L. and L. sativa var. capitata L.) related to the preliminary phytochemical investigation, nutrient content, DPPH assay, and in vitro anticoagulant activity by determination of prothrombin time (PT) and activated partial thromboplastin time (aPTT). The findings revealed almost the same phytoconstituents in the leaves and stems of each variety, such as terpenoids, flavonoids, coumarins, and others. Exceptionally, tannins have only been detected in the leaves. Compared to the stems, the leaves of both varieties showed statistically significantly higher levels of raw protein and raw fat. For the DPPH assay, the leaves of both varieties have a statistically significantly higher antioxidant activity than the stems. The leaves and stem extract of the two selected varieties showed a significant prolongation of PT (P <0.05 vs. NS= 12:30 s). On the other hand, the aPTT test showed a significant increase in aPTT (P <0.05 vs. NS = 32:44 s) in the stem extract of both varieties, whereas there was no statistically significant increase in aPTT in the leaves of both varieties. Both parts of each variety have a diversity of phytochemicals and nutrients. The leaves of both varieties have a higher antioxidant activity than the stems. While the stems had higher anticoagulant activity than the leaves.

scholarly journals EVALUATION OF ANTHELMINTIC ACTIVITY OF CITRUS RETICULATA: IN VITRO AND ITS PHYTOCHEMICAL INVESTIGATION

2017 ◽  
Vol 10 (5) ◽  
pp. 278
Author(s):  
Arjan Aryal
Keyword(s):  
Methanolic Extract ◽  
Anthelmintic Activity ◽  
Volatile Oil ◽  
Citrus Reticulata ◽  
In Vivo Models ◽  
Standard Drug ◽  
Phytochemical Investigation ◽  
Vitro Model

Background: The prevalence of worm infestation is high in under developed and developing countries due to poor sanitation and lack of health education. Citrus reticulata, fruit belonging to citrus family rutaceae is a common fruit native to Asia. Peels of the fruit is rich in limonene, a potent phytoconstituent having anthelmintic activity. However, proper utilization of peels has not been done as it is not consumed and is therefore discarded.Objective: To explore various phytoconstituents present in Citrus reticulata and its anthelmintic effect.Method: Various concentration of Methanolic extract and Volatile oil of Citrus reticulata were subjected for assessment of anthelmintic activity in earthworms. Time of paralysis and time of death were used as an evaluation parameter. Albendazole (25 mg/ml) was used as a standard drug. Results: Phytochemical test revealed the presence of alkaloids, carbohydrates, tannins, flavonoids, terpenoids and glycosides. Concentration dependent anthelmintic effect was observed with the extract where 150 mg/ml concentration of methanolic extract showed paralysis of test worm (earthworm) at 5.76 min and death at 19.16 min respectively.Conclusion: Citrus reticulata peel has shown substantial anthelmintic activity using in vitro model on earthworms. Hence, further research is required to understand its mechanism of action using in vivo models to confirm its anthelmintic potential.

scholarly journals Comparative In vitro Anthelmintic Activity of Mentha piperita and Lantana camara from Western India

1970 ◽  
Vol 5 (1) ◽  
pp. 5-7 ◽  
Author(s):  
AS Girme ◽  
RD Bhalke ◽  
PB Ghogare ◽  
VD Tambe ◽  
RS Jadhav ◽  
...  
Keyword(s):  
Anthelmintic Activity ◽  
Lantana Camara ◽  
Mentha Piperita ◽  
Western India ◽  
Time Of Death ◽  
Reference Drug ◽  
Methanol Extracts ◽  
And Control

Methanol extracts from the leaves, stems and roots of Mentha piperita and Lantana camara were investigated for their anthelmintic activity against Pheritima posthuma. Each extract was studied in the bioassay at 20 mg/ml, which involved determination of time of paralysis and time of death of the worms. Both the extracts of both plants exhibited considerable anthelmintic activities, and the order of sensitivity of the extracts to the worms was stems > roots > leaves for the M. piperita and stems > leaves >roots for the L. camara. The methanol extracts of stems of both M. piperita and L. camara were found to be the most active. Albendazole (20 mg/ml) and distilled water were included in the assay as standard reference drug and control, respectively. Key words: Mentha piperita, Lantana camara, Pheritima posthuma, In vitro anthelmintic activity. Dhaka Univ. J. Pharm. Sci. Vol.5(1-2) 2006 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals In vitro Anthelmintic Activity of Leaf Extracts of Adhatoda vasica Nees (Acanthaceae) Against Eudrilus eugeniae

2016 ◽  
Vol 14 (2) ◽  
pp. 153-155
Author(s):  
Somnath D Bhinge ◽  
Prachi Desai ◽  
Chandrakant S Magdum
Keyword(s):  
Anthelmintic Activity ◽  
Negative Control ◽  
Active Principle ◽  
Aqueous Extracts ◽  
Eudrilus Eugeniae ◽  
Leaf Extracts ◽  
Adhatoda Vasica ◽  
Standard Drug

The present study specifically indicated that the crude ethanolic and aqueous extracts of the leaves of Adhatoda vasica Nees produced anthelmintic activity against african earthworm Eudrilus eugeniae. Various concentrations (10, 25, 50 mg/ml) of aqueous and ethanolic extracts were evaluated in the bioassay involving determination of time of paralysis (P) and time of death (D) of the worms. Albendazole was used as standard anthelmintic drug and distilled water was used as negative control. The results of the present study indicated that the ethanolic and aqueous extracts significantly exhibited paralysis of worms in lower doses (10, 25 and 50 mg/ml) and also caused death of worms at higher concentration of 50 mg/ml, as compared to standard drug. Further studies are in process to isolate the active principle responsible for the activity.Dhaka Univ. J. Pharm. Sci. 14(2): 153-155, 2015 (December)

IN VITRO ANTHELMINTIC ACTIVITY OF BARK EXTRACT OF FICUS GLOMERATA ROXB. AGAINST EUDRILUS EUGENIAE

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (08) ◽  
pp. 9-11
Author(s):  
M. G Hogade ◽  
◽  
B. N Poul ◽  
S. S Kuthar ◽  
C. V. Panchal ◽  
...  
Keyword(s):  
Anthelmintic Activity ◽  
Bark Extract ◽  
Aqueous Extracts ◽  
Time Of Death ◽  
Eudrilus Eugeniae ◽  
Standard Drug ◽  
Ethanolic Extracts ◽  
Ficus Glomerata

The present study was undertaken to evaluate anthelmintic activity of ethanolic and aqueous bark extracts of Ficus glomerata against Eudrilus eugeniae. For this study, we used various concentrations (25-100 mg/mL) of aqueous and ethanolic extracts and these were evaluated in the bioassay involving determination of time of paralysis (P) and time of death (D) of the worms. Albendazole was used as standard anthelmintic drug and distilled water was used as control. The results of present study indicate that the ethanolic and aqueous extracts significantly exhibited paralysis (P<0.01) in worms in lower doses (25, 50 and 100 mg/mL) and also caused death of worms, especially at higher concentration of 100 mg/mL, as compared to standard drug.

scholarly journals Evaluation of in vitro anthelmintic activity of extracts of jasminum sessiliflorum

2019 ◽  
Vol 10 (3) ◽  
pp. 2542-2544
Author(s):  
Ruby Philip ◽  
Kathiresan Krishnasamy ◽  
Elessy Abraham
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Significant Activity ◽  
Standard Drug ◽  
Death Time ◽  
Ancient People ◽  
Ancient Times ◽  
The Common

Plant preparations are most popular from ancient times for the treatment of various diseases. The ancient people had an appreciable on the application and usage of plant preparations for the common ailments. Based on the investigation, it has been found that various species of this plant has been used as an anthelmintic. The extracts of Jasminum sessiliflorum was investigated for anthelmintic potential using earthworms, Pheretima posthuma. Different concentration of plant extracts were used for the evaluation. Albendazole (10 mg/ml) was used as reference standard drug. The method employs the determination of paralysis time and death time of the worms and these results were recorded. Extracts showed significant activity. The ethanolic extract was found to be most efficient.

Evaluation of antioxidant and Anthelmintic activity of methanolic flower extract of Nyctanthes arbor-tristis

2018 ◽  
Vol 9 (1) ◽  
pp. 78
Author(s):  
Vijaya Jyothi M ◽  
Bhargav E ◽  
Pavan Kumar K ◽  
Praneeth Gowd K ◽  
Ram Pavan S
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Iridoid Glycosides ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Total Phenolic ◽  
Standard Drug ◽  
Flower Extract ◽  
Reducing Ability ◽  
Total Flavonoids Content

Nyctanthes arbour-tristis is a shrub belongs to the family Oleaceae. The flowers of this plant are fragrant since the presence of flavonol glycosides. It has also been reported for the presence of β-sitosterol, iridoid glycosides, tannins etc., and known to have immunostimulant, hepatoprotective, antiviral and antifungal activities. In the present study an attempt is made to identify antioxidant capacity and anthelminthic potential of methanolic flower extract of Nyctanthes arbour-tristis. Antioxidant activity was evaluated by total phenolic content assay, total flavonoids content assay, free radical scavenging activity and reducing ability methods. Anthelmintic activity was evaluated on Perithima posthuma using Piperazine citrate as standard drug. The results obtained for the above activities reveals that Nyctanthes arbour-tristis shows considerable antioxidant activity for all the methods and anthelminthic potential at 300 mg/ml. Keywords: arbour-tristis; antioxidant activity; anthelminthic activity; Perithima posthuma; Piperazine citrate.

Design, Synthesis and Pharmacological Evaluation of New Thiazole Derivatives as Anthelmintic Agents

2020 ◽  
Vol 13 (5) ◽  
pp. 5075-5081
Author(s):  
Sirisha Kalam ◽  
Sai Krishn G ◽  
Kumara Swamy D ◽  
Sai Santhoshi K ◽  
Durga Prasad K
Keyword(s):  
Anthelmintic Activity ◽  
Docking Studies ◽  
Molecular Property ◽  
Thiazole Derivatives ◽  
Pharmacological Agents ◽  
Design Synthesis ◽  
Standard Drug ◽  
Lipinski’S Rule ◽  
Mass Spectral ◽  
Tubulin Protein

Pharmacological agents that kills parasites are essential drugs in some tropical countries. In this study, a series of 2-amino substituted 4-phenyl thiazole derivatives (4a-e) have been synthesized by the conventional method. The thiazole derivatives were synthesized by three steps. The obtained five derivatives were purified by recrystallization using methanol as a solvent or column chromatography. They were characterized by melting point, TLC, FTIR, 1H NMR and MASS spectral data. Compounds 4a-e were evaluated in silico by using different software’s (Lipinski’s Rule of 5, OSIRIS molecular property explorer, Molsoft molecular property explorer, and PASS & docking studies). These compounds were then evaluated for their possible anthelmintic activity against Indian adult earth worms (Pherituma postuma). All the compounds displayed significant anthelmintic activity. Compound 4c and 4e were more potent compounds when compared with the standard drug (mebendazole). Molecular docking studies guided and proved the biological activity against beta tubulin protein (1OJ0). In conclusions, these new molecules have promising potential as anthelmintic for treatment of parasites.   

Phytochemical Constituents of Annona reticulata and their Cytotoxic Activity

2020 ◽  
Vol 17 (3) ◽  
pp. 206-210
Author(s):  
Ty Viet Pham ◽  
Thang Quoc Le ◽  
Anh Tuan Le ◽  
Hung Quoc Vo ◽  
Duc Viet Ho
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Structural Determination ◽  
Phytochemical Investigation ◽  
Ic50 Values ◽  
Phytochemical Constituents ◽  
First Time ◽  
Annona Reticulata

A phytochemical investigation of the leaves of Annona reticulata led to the isolation and structural determination of β-sitosterol (1), ent-pimara-8(14),15-dien-19-oic acid (2), ent-pimara- 8(14),15-dien-19-ol (3), quercetin (4), quercetin 3-O-α-L-arabinopyranoside (5), and a mixture of quercetin 3-O-β-D-galactopyranoside (6a) and quercetin 3-O-β-D-glucopyranoside (6b). Of these, compounds 2 and 3 were isolated from the genus Annona for the first time. Compound 3 showed strong cytotoxicity against SK-LU-1 and SW626 cell lines with IC50 values of 17.64 ± 1.07 and 19.79 ± 1.41 μg mL-1, respectively.

scholarly journals Antimicrobial, Antioxidant, Anti-Acetylcholinesterase, Antidiabetic, and Pharmacokinetic Properties of Carum carvi L. and Coriandrum sativum L. Essential Oils Alone and in Combination

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3625
Author(s):  
Hafedh Hajlaoui ◽  
Soumaya Arraouadi ◽  
Emira Noumi ◽  
Kaïss Aouadi ◽  
Mohd Adnan ◽  
...  
Keyword(s):  
Essential Oils ◽  
Coriandrum Sativum ◽  
New Drugs ◽  
Antidiabetic Activity ◽  
Pharmacokinetic Analysis ◽  
Bacterial Strains ◽  
Standard Drug ◽  
Reference Drug ◽  
Antioxidant Power ◽  
Carum Carvi

Herbs and spices have been used since antiquity for their nutritional and health properties, as well as in traditional remedies for the prevention and treatment of many diseases. Therefore, this study aims to perform a chemical analysis of both essential oils (EOs) from the seeds of Carum carvi (C. carvi) and Coriandrum sativum (C. sativum) and evaluate their antioxidant, antimicrobial, anti-acetylcholinesterase, and antidiabetic activities alone and in combination. Results showed that the EOs mainly constitute monoterpenes with γ-terpinene (31.03%), β-pinene (18.77%), p-cymene (17.16%), and carvone (12.20%) being the major components present in C. carvi EO and linalool (76.41%), γ-terpinene (5.35%), and α-pinene (4.44%) in C. sativum EO. In comparison to standards, statistical analysis revealed that C. carvi EO showed high and significantly different (p < 0.05) antioxidant activity than C. sativum EO, but lower than the mixture. Moreover, the mixture exhibited two-times greater ferric ion reducing antioxidant power (FRAP) (IC50 = 11.33 ± 1.53 mg/mL) and equipotent chelating power (IC50 = 31.33 ± 0.47 mg/mL) than the corresponding references, and also potent activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 19.00 ± 1.00 mg/mL), β-carotene (IC50 = 11.16 ± 0.84 mg/mL), and superoxide anion (IC50 = 10.33 ± 0.58 mg/mL) assays. Antimicrobial data revealed that single and mixture EOs were active against a panel of pathogenic microorganisms, and the mixture had the ability to kill more bacterial strains than each EO alone. Additionally, the anti-acetylcholinesterase and α-glucosidase inhibitory effect have been studied for the first time, highlighting the high inhibition effect of AChE by C. carvi (IC50 = 0.82 ± 0.05 mg/mL), and especially by C. sativum (IC50 = 0.68 ± 0.03 mg/mL), as well as the mixture (IC50 = 0.63 ± 0.02 mg/mL) compared to the reference drug, which are insignificantly different (p > 0.05). A high and equipotent antidiabetic activity was observed for the mixture (IC50 = 0.75 ± 0.15 mg/mL) when compared to the standard drug, acarbose, which is about nine times higher than each EO alone. Furthermore, pharmacokinetic analysis provides some useful insights into designing new drugs with favorable drug likeness and safety profiles based on a C. carvi and C. sativum EO mixture. In summary, the results of this study revealed that the combination of these EOs may be recommended for further food, therapeutic, and pharmaceutical applications, and can be utilized as medicine to inhibit several diseases.

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