Occurrence of intestinal parasites among the teachers, students and staffs of Dhaka University

The present study was conducted to find out the incidence of the intestinal parasites and their prevalence among the teachers, students and staffs of University of Dhaka, Bangladesh. The study revealed that the prevalence of intestinal protozoa and helminth parasites are common among the outdoor patients of University of Dhaka. A total of 350 stool samples was examined in the Pathology department of Medical Center of University of Dhaka from June 2009 to May 2010, four species of intestinal parasites were identified of which two species were protozoa (Entamoeba histolytica and Giardia intestinalis) and two species were nematodes (Ascaris lumbricoides and Trichuris trichiura). The overall prevalence of infestation was 23.14% where Entamoeba histolytica, Giardia intestinalis, Ascaris lumbricoides and Trichuris trichiura were found as 4.86%, 3.71%, 11.14% and 3.43% respectively. Highest prevalence was recorded in Ascaris lumbricoides (11.14%) and the seasonal pattern showed that highest (30%) prevalence occurred in rainy season and lowest (17.19%) in winter season. The prevalence of intestinal parasites was higher in female (30.56%) than in male (22.29%). DOI: http://dx.doi.org/10.3329/jasbs.v39i2.17863 J. Asiat. Soc. Bangladesh, Sci. 39(2): 239-246, December 2013

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Personal hygiene practices and socio-economic conditions as influential factors for intestinal parasitic infection in Dhaka city dwellers

Bangladesh Journal of Zoology ◽

10.3329/bjz.v47i1.42028 ◽

2019 ◽

Vol 47 (1) ◽

pp. 129-136

Author(s):

Md Aminul Islam Bhuiyan ◽

Zannatun Nahar Jhinu ◽

Jaliluzzaman ◽

Mandira Mukutmoni

Keyword(s):

Entamoeba Histolytica ◽

Ascaris Lumbricoides ◽

Intestinal Parasites ◽

Parasitic Infection ◽

Trichuris Trichiura ◽

Giardia Intestinalis ◽

Personal Hygiene ◽

Parasitic Infections ◽

Variable Degree ◽

Hygiene Practices

A total of 200 stool samples were examined and five species of intestinal parasites were identified. Two of them were protozoans (Entamoeba histolytica and Giardia intestinalis) and the rest of which three species were nematode parasites (Ascaris lumbricoides, Trichuris trichiura and Strongyloids stercoralis). The overall prevalence of parasitic infection was 50%. Individually the prevalence of Ascaris lumbricoides, Entamoeba histolytica, Giardia intestinalis, Trichuris trichiura and Strongyloids stercoralis was 25%, 13%, 6%, 3.5% and 2.5%, respectively. Patients of different age and sex had a variable degree of infection. Children aged under ten years showed the highest prevalence (73.97%) compared to other groups. Accordingly, male patients were found slightly more susceptible to the infection (50.38%) compared to the female (49.29%). People belonging to different occupation and educational qualification had various degree of infection. As expected patients who had no education and poor monthly income, showed the highest prevalence of infection, 77.77% and 90%, respectively. Personal hygiene practices of the patients had much influence on the parasitic prevalence and rate of infection. Respectively 58.82%, 91.42%, 80% and 63.55% prevalence were found among the patients who used to drink unsafe water, wash hands without soap, stay barefooted most of the time and do not trim their nails regularly. The present study supposed to reveal that socioeconomic conditions and behavioral factors may influence the prevalence of parasitic infections. Bangladesh J. Zool. 47(1): 129-136, 2019

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Urban versus rural prevalence of intestinal parasites using multi-parallel qPCR in Colombia

10.1101/2020.09.20.20197616 ◽

2020 ◽

Author(s):

Patricia E Bryan ◽

Marcela Romero ◽

Miryan Sanchez ◽

Giovanny Torres ◽

Wilber Gomez ◽

...

Keyword(s):

Ascaris Lumbricoides ◽

Rural Areas ◽

Intestinal Parasites ◽

Parasite Burden ◽

Strongyloides Stercoralis ◽

Urban Slum ◽

Trichuris Trichiura ◽

Giardia Intestinalis ◽

Epidemiologic Surveillance ◽

Blastocystis Spp

Stool samples from 122 children from urban slum (n = 72) and rural (n = 50) areas were analyzed using multi-parallel real-time qPCR to determine the prevalence of intestinal parasites from two communities in Colombia. Findings indicated a prevalence of 86.1% Blastocystis spp., 62.5% Giardia intestinalis, 19.4% Cryptosporidium spp., 19.4% Ascaris lumbricoides, and 5.6% Trichuris trichiura in an urban slum; and 76% Blastocystis spp., 68% Giardia intestinalis, 20% Entamoeba histolytica, 50% Ascaris lumbricoides, 46% Trichuris trichiura and 2% Strongyloides stercoralis in rural areas. Polyparasitism was higher in rural (58%) compared to urban (25%) areas (p = 0.001). Trichuris trichiura burden was higher in the rural area (p = 0.002). Over 40% of helminth infections in rural areas had a heavy parasite burden by WHO classification. Over half of urban and rural children were infected with Giardia intestinalis and Blastocystis spp. Our data provides accurate epidemiologic surveillance for public health interventions.

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Estandarización de una reacción en cadena de la polimerasa en tiempo real (qPCR) para la detección de Strongyloides stercoralis en muestras de materia fecal

Medicina y Laboratorio ◽

10.36384/01232576.94 ◽

2016 ◽

Vol 22 (9-10) ◽

pp. 459-478

Author(s):

Laura F. Campo-Polanco ◽

José M. Hernández-Sarmiento ◽

Luz E. Botero-Palacio ◽

Lina A. Gutiérrez-Builes

Keyword(s):

Entamoeba Histolytica ◽

Ascaris Lumbricoides ◽

Strongyloides Stercoralis ◽

Trichuris Trichiura ◽

Giardia Intestinalis ◽

Hymenolepis Nana ◽

Entamoeba Dispar

Introducción: el diagnóstico de estrongiloidiasis se realiza de rutina en los laboratorios clínicos; sin embargo, su detección se dificulta debido a la baja excreción parasitaria y la baja sensibilidad de las pruebas parasitológicas empleadas. Objetivo: diseñar y estandarizar una PCR en tiempo real (qPCR) para la detección de ADN de Strongyloides stercoralis en muestras de materia fecal. Materiales y métodos: se establecieron las condiciones de qPCR y se evaluaron: a) la especificidad analítica mediante análisis BLASTn de secuencias obtenidas de muestras positivas para Strongyloides stercoralis, b) sensibilidad analítica mediante diluciones seriadas de muestras que contenían larvas de Strongyloides stercoralis y c) la ocurrencia de reacciones cruzadas con otros parásitos e inhibidores de la amplificación. Resultados: se amplificó un fragmento de 101 pb del gen 18S del ARN ribosomal. El valor de Ct osciló entre 23 y 29, tomando un Ct ≤35 como el punto de corte para muestras positivas. El análisis BLASTn de las secuencias obtenidas mostró un porcentaje de identidad del 98% con secuencias 18S del ARN ribosomal de Strongyloides stercoralis reportadas en la NCBI. El límite inferior de detección de la qPCR fue 0,9 ng/µL. No se evidenció reacción cruzada con Ascaris lumbricoides, Trichuris trichiura, Uncinarias, Hymenolepis nana, Entamoeba histolytica/Entamoeba dispar, Entamoeba hartmanni, Giardia intestinalis e Iodamoeba bütschlii. No se detectaron inhibidores en las muestras de materia fecal. Conclusiones: la sensibilidad y la especificidad analítica de la qPCR comparado con el examen directo de heces son del 100%; sin embargo, aún no es posible interpretar su utilidad clínica.

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Intestinal Parasitic Infestation among the Outdoor Patients of Dhaka University Medical Centre, Bangladesh

University Journal of Zoology Rajshahi University ◽

10.3329/ujzru.v28i0.5285 ◽

1970 ◽

Vol 28 ◽

pp. 45-49 ◽

Cited By ~ 1

Author(s):

Hamida Khanum ◽

Md Mizanur Rahman ◽

Md Hafiz Uddin ◽

Shahela Alam ◽

Fatema Rahman ◽

...

Keyword(s):

Female Patient ◽

Entamoeba Histolytica ◽

Ascaris Lumbricoides ◽

Intestinal Parasites ◽

Parasitic Infection ◽

Giardia Intestinalis ◽

Medical Centre ◽

Parasitic Infestation ◽

Teacher Student ◽

Stool Samples

In the present study, the prevalence of intestinal parasitic infestation was investigatedamong the outdoor patients including teacher, student and staff of the Dhaka University treated atDhaka University Medical Centre. A total of 380 stool samples were examined. Four species ofintestinal parasites were identified, two protozoans (Entamoeba histolytica and Giardia intestinalis)and two nematodes (Ascaris lumbricoides and Trichuris trichiura). The prevalence of parasiticinfestation was 24.73%. The prevalence of E. histolytica was 3.95%, G. intestinalis 6.31%,A. lumbricoides 11.84% and T. trichiura 2.63%. The prevalence was higher in case of staff(32.31%) and lower in case of teacher (13.16%). Highest prevalence observed during the rainyseason (29.3%) and lowest in winter (19.4%). Female patient showed higher prevalence (31.25%)compared to male (24.14%). The rate of infection was higher (28.3%) among the patients, whoused to drink unboiled water. The present study revealed that parasitic infection among the outdoorpatients of Dhaka University Medical Centre is alarmingly high.Key words: Dhaka University Medical Centre; intestinal parasites; outdoor patientsDOI: 10.3329/ujzru.v28i0.5285Univ. j. zool. Rajshahi Univ. Vol. 28, 2010 pp. 45-49

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Parasitosis intestinal con predominio de flagelados comensales, en indígenas Waraos, estado Delta Amacuro, Venezuela

Gaceta Medica Boliviana ◽

10.47993/gmb.v41i1.140 ◽

2020 ◽

Vol 41 (1) ◽

pp. 10-13

Author(s):

Luis Traviezo Valles ◽

Flor Moraleda Rivero ◽

Noelis Rivas Pinto

Keyword(s):

Entamoeba Histolytica ◽

Ascaris Lumbricoides ◽

Giardia Lamblia ◽

Trichuris Trichiura ◽

Giardia Intestinalis ◽

Entamoeba Dispar ◽

Balantidium Coli ◽

Blastocystis Sp ◽

Pentatrichomonas Hominis

Las poblaciones de indígenas Waraos del Bajo Delta, adolecen de servicios sanitarios óptimos que le permitan diagnosticar y prevenir las enteroparasitosis. Objetivo: determinar la Prevalencia de Parasitosis Intestinal (PPI), frecuencia y diversidad de especies de los enteroparásitos presentes, con énfasis en los flagelados comensales. Métodos: se estudiaron 51 pacientes de la Etnia Warao (21 del sexo femenino y 30 masculino) con edades comprendidas entre los 2 meses y los 68 años, a los cuales se le realizaron exámenes coproparasitológicos, con las técnicas de la solución salina 0,85%, lugol y la técnica de Kato. Resultados: se obtuvo un PPI del 100%, donde los protozoarios diagnosticados fueron: Endolimax nana (58,8% de las muestras analizadas), Blastocystis sp. (56,9%), Entamoeba histolytica/Entamoeba dispar (41,2%), Entamoeba coli (29,4%), Iodamoeba butschlii (25,5%), Pentatrichomonas hominis/Trichomonas hominis (17,6%), Giardia lamblia /Giardia intestinalis (15,7%), Chilomastix mesnili (11,8%), Balantidium coli (2%) y Entamoeba hartmani (2%). Los helmintos encontrados fueron: Ascaris lumbricoides (25,5%), Trichuris trichiura (5,9%) y Uncinarias sp. (2%). Conclusiones: la PPI y la frecuencia de Pentatrichomonas hominis y Chilomastix mesnili son de las más altas reportadas para Venezuela en los últimos años, indicadores de la difícil situación sanitaria de esta etnia.

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OCCURRENCE OF Blastocystis spp. IN UBERABA, MINAS GERAIS, BRAZIL

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652015000300005 ◽

2015 ◽

Vol 57 (3) ◽

pp. 211-214 ◽

Cited By ~ 11

Author(s):

Marlene CABRINE-SANTOS ◽

Eduardo do Nascimento CINTRA ◽

Rafaela Andrade do CARMO ◽

Gabriel Antônio Nogueira NASCENTES ◽

André Luiz PEDROSA ◽

...

Keyword(s):

Public Health ◽

Entamoeba Histolytica ◽

Ascaris Lumbricoides ◽

Intestinal Parasite ◽

Intestinal Parasites ◽

Giardia Intestinalis ◽

High Agreement ◽

Direct Microscopy ◽

The World ◽

Blastocystis Spp

Intestinal parasites are a problem for public health all over the world. The infection with Blastocystis, a protozoan of controversial pathogenicity, is one of the most common among them all. In this study, the occurrence of intestinal parasites, with emphasis on Blastocystis, in patients at the Universidade Federal do Triângulo Mineiro was investigated in Uberaba (MG) through microscopy of direct smears and fecal concentrates using Ritchie’s method. Feces of 1,323 patients were examined from April 2011 to May 2012. In 28.7% of them at least one intestinal parasite was identified, and the most frequent organisms were Blastocystis spp. (17.8%) and Giardia intestinalis (7.4%). The occurrence of parasitism was higher in children aged 6 -10 years old, and the infection with Blastocystis spp. was higher above the age of six (p < 0.001). The exclusive presence of G. intestinalis and of Blastocystis spp. was observed in 5.4% and 12.2% of the patients, respectively. Regarding patients with diarrheic feces, 8% revealed unique parasitism of Blastocystis spp. Other intestinal parasites observed in children were Ascaris lumbricoides (0.3%) and Entamoeba histolytica/dispar/moshkovskii (1.4%). The Ritchie’s method was more sensitive (92.8%) when compared to direct microscopy (89.8%), with high agreement between them (97.7%, kappa = 0.92). In conclusion, the occurrence of Blastocystis spp. in Uberaba is high and the presence of diarrheic feces with exclusive presence of the parasite of Blastocystis spp. was observed.

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Prevalence of Intestinal Parasitic Infestation Among Hearing and Speech Impaired Children of Banke, Nepal

Journal of Nepalgunj Medical College ◽

10.3126/jngmc.v16i2.24877 ◽

2018 ◽

Vol 16 (2) ◽

pp. 44-46

Author(s):

Maya Rai ◽

Ganesh Prasad Neupane ◽

Dabal Bahadur Dhami ◽

Thakur Prasad Paudel

Keyword(s):

Entamoeba Histolytica ◽

Ascaris Lumbricoides ◽

Giardia Lamblia ◽

Intestinal Parasites ◽

Parasitic Infection ◽

Life Quality ◽

Wet Mount ◽

The Common ◽

Impaired Children ◽

Prompt Diagnosis

Introduction: The parasites can cause different gastrointestinal disorders which have great impact on life quality. Hearing and speech impaired children are unable to maintain proper sanitation, making them more prone to intestinal parasitic infection. Aim and Objective: To know the prevalence of various parasitic infestations among hearing and speech impaired Children. Materials and Methods: Stool specimen were collected from 104 hearing and speech impaired children who were living in private rehabilitation school of chisapani village of Banke district Nepal for a period of 5 month from December 2017 to April 2018 and direct wet mount was prepared using normal saline (0.9%) and Lugol's iodine (0.5%). The wet mount was observed under microscope for parasites. Results: Among 104 individuals intestinal parasites were seen in 25.96 % of students. Giardia lamblia (37.04%) was the most common parasite followed by Ascaris lumbricoides (18.52%) and Entamoeba histolytica (14.82%). Conclusion: The study shows that although the speech and hearing impaired children are more prone to parasitic infection, the prevalence is same as normal people. Giardia lamblia, Ascaris lumbricoides and Entamoeba histolytica were the common parasites. Prompt diagnosis and treatment of infected children should be undertaken.

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Prevalence of Giardia intestinalis Infection in Schistosomiasis-Endemic Areas in South-Central Mali

Tropical Medicine and Infectious Disease ◽

10.3390/tropicalmed4020086 ◽

2019 ◽

Vol 4 (2) ◽

pp. 86 ◽

Cited By ~ 1

Author(s):

Hassan K.M. Fofana ◽

Maren Schwarzkopf ◽

Mama N. Doumbia ◽

Rénion Saye ◽

Anna Nimmesgern ◽

...

Keyword(s):

Intestinal Parasites ◽

Epidemiological Data ◽

Giardia Intestinalis ◽

Diagnostic Tools ◽

Intestinal Protozoa ◽

Control Programs ◽

South Central ◽

Concurrent Infections ◽

Intestinal Pathogens ◽

Stool Samples

Intestinal parasite infections are frequent causes of diarrhea and malnutrition among children in the tropics. Transmission of helminths and intestinal protozoa is intimately connected with conditions of poverty, including inadequate sanitation and hygiene. Concurrent infections with several intestinal pathogens may lead to excess morbidity. Yet, there is a paucity of epidemiological data from Mali. In this study, stool samples from 56 individuals, aged 2–63 years, from Bamako and Niono, south-central Mali were examined for intestinal parasites using stool microscopy. Additionally, stool samples were subjected to a rapid diagnostic test (RDT) and polymerase chain reaction (PCR) for the detection of Cryptosporidium spp. and Giardia intestinalis. The predominant pathogens were Schistosoma mansoni and G. intestinalis with prevalences of 41% and 38%, respectively. Hymenolepis nana was detected in 4% of the participants, while no eggs of soil-transmitted helminths were found. Concurrent infections with G. intestinalis and S. mansoni were diagnosed in 16% of the participants. For the detection of G. intestinalis, PCR was more sensitive (100%) than RDT (62%) and microscopy (48%). As helminth-protozoa coinfections might have important implications for morbidity control programs, future studies should employ diagnostic tools beyond stool microscopy to accurately assess the co-endemicity of giardiasis and schistosomiasis.

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Parasitosis Intestinal En Una Población De 5 A 14 Años Que Acuden A Unidades Educativas Escuelas Colegios Públicos De La Ciudad De Riobamba

European Scientific Journal ESJ ◽

10.19044/esj.2017.v13n30p11 ◽

2017 ◽

Vol 13 (30) ◽

pp. 11

Author(s):

Sandra Noemí Escobar Arrieta ◽

Verónica Mercedes Cando Brito ◽

Carlos Eduardo Espinoza Chavez ◽

Luis Elias Guevara Iñiguez

Keyword(s):

Risk Factors ◽

Public Schools ◽

Ascaris Lumbricoides ◽

Saline Solution ◽

Intestinal Parasites ◽

Educational Institution ◽

Parasite Prevalence ◽

Trichuris Trichiura ◽

Hymenolepis Nana ◽

High Prevalence

The purpose of the current research was to determine the prevalence and risk factors linked to intestinal parasites in a population aged from 5 to 14 years who attend to public schools and high schools from Riobamba city, Riobamba-Chambo district. The prospection was carried out between October and November 2015. The population studied was identified and classified according to their age, gender, and educational institution they belong to. In addition, the relation of risk factors linked to the identified parasites was estimated. For this, it was necessary to apply a coprology examination to 2020 boys, girls and teenagers. The fecal samples collected were analyzed using the fresco technique with lugol and saline solution at 0.85%. The total intestine parasite prevalence found was 35.1%. The highest parasite prevalence was detected in groups aged 5 (12.84%), in groups aged 8 (11.71%) and in groups aged 11 (11.56%), while Combatientes de Tapi Educational Unit registered a 34% in both genders. Any significant parasite difference was found among p<0.05 ages. Nine parasite types were identified with Entamoeba coli prevalence (26%) Entamoeba histolytic (9.3%), Iodamoeba bütschlii (0.8%), Giardia lambia (4.3%), Chilomastix mesnilii (0.9%), Hymenolepis nana (1.4%), Ascaris lumbricoides (0.3%) Trichuris trichiura (0.5%), Endolimax nana (1.6%), from which protozoan were the predominant, some poly parasite samples were also found, it was determined that the sample studied reflects a high prevalence of intestinal parasites.

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Enteroparasitismo en indígenas Terena en el estado de Mato Grosso do Sul, Brasil

Revista de Salud Pública ◽

10.15446/rsap.v16n6.40031 ◽

2015 ◽

Vol 16 (6) ◽

pp. 859-870 ◽

Cited By ~ 1

Author(s):

Antonio Neres Norberg ◽

Fabiano Guerra-Sanches ◽

Paulo R. Blanco Moreira-Norberg ◽

José Tadeu Madeira-Oliveira ◽

Aluízio Antonio Santa-Helena ◽

...

Keyword(s):

Entamoeba Histolytica ◽

Ascaris Lumbricoides ◽

Giardia Lamblia ◽

Strongyloides Stercoralis ◽

Trichuris Trichiura ◽

Enterobius Vermicularis ◽

Hymenolepis Nana ◽

Mato Grosso ◽

Mato Grosso Do Sul

Objetivo Considerando que más de la mitad de la población mundial está infectada por protozoarios y helmintos intestinales, con alta prevalencia en las zonas más pobres, esta investigación tuvo como objetivo identificar la prevalencia de los parasitismos entre indígenas de la etnia Terena, establecidos en el Estado de Mato Grosso do Sul.Metodología Se examinaron 134 muestras de heces de individuos de la comunidad indígena. Estas se conservaron en solución de Merthiolate-iodo-formol (MIF). Los exámenes de laboratorio fueron realizados por las técnicas de Hoffman, Pons y Janer; Willis y Kinyoun.Resultados Se identificaron infecciones por helmintos nematodos de las especies Ascaris lumbricoides, Ancylostomidae, Enterobius vermicularis, Strongyloides stercoralis, y Trichuris trichiura; cestodos de la especie Hymenolepis nana, y Taeniaspp. También por protozoarios de las especies Cryptosporidium spp., Giardia lamblia, Endolimax nana, Entamoeba coli, y Entamoeba histolytica. De las muestras investigadas, 23,1% fueron negativas; de los 76,9 % parasitados hubo diferencia estadísticamente no significativa para el parasitismo en hombres y mujeres examinados, de unoa 33 años de edad, y sobre parasitismo mono específico, o con simultaneidad de especies. Como diversidad parasitaria fueron encontradas siete especies de helmintos nematodos y cestodos, y cinco de protozoarios Archamoebae, flagelados y enterozoários. Conclusiones Los resultados fueron la base para la orientación e intervención adecuada, revelando la necesidad de la implantación de medidas gubernamentales y socioeducativas para mejorar las condiciones de vida de esta comunidad.

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