scholarly journals EFFECT OF COW URINE TREATMENT ON PLANT GROWTH AND ANTI-MICROBIAL ACTIVITY ON GOSSYPIUM HIRSUTUM L.

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
S. Krishnaveni ◽  
M. Mamatha
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Gossypium Hirsutum ◽  
Shoot Length ◽  
The Other ◽  
Diffusion Method ◽  
Cow Dung ◽  
Gossypium Hirsutum L ◽  
Cow Urine ◽  
Agar Diffusion Method

In Hindu culture, the cow is treated as a divine animal. Cow's urine, cow dung and Milk are utilized for different purposes. Cow's urine has been utilized as an enhancer of plant growth and an antifungal agent for the current study. The plant selected for this study Gossypium hirsutum L.(cotton).The plant was grown utilizing various concentrations of cow urine, such as 0% (control), 1:10, 1:5, 1:1 for 90 days. Various parameters for example leaf area, shoot length, plant height, root length, as well as biomass of the plant have been monitored. The antifungal activity has been studied by utilizing different concentrations of cow urine against (Alternaria macrospora) using agar diffusion method. Among all the concentrations, the 1:1 concentration showed maximum plant growth compared to other two concentrations. On the other hand, 1:5, 1:10 concentrations showed more antifungal activity. The conclusion of this study found that cow urine can be utilized as a growth enhancer of plant and has antifungal properties.A

scholarly journals Cow Urine: Plant Growth Enhancer and Antimicrobial Agent

2019 ◽  
Vol 8 ◽  
pp. 30-45
Author(s):  
Karthikeya Srinivasa Varma Gottimukkala ◽  
Bishwambhar Mishra ◽  
Sampadha Joshi ◽  
Madhu Karan Reddy
Keyword(s):  
Plant Growth ◽  
Antimicrobial Agent ◽  
Maximum Growth ◽  
Urine Concentration ◽  
Culture Technique ◽  
Diffusion Method ◽  
Cow Dung ◽  
Cow Urine ◽  
Urine Concentrations ◽  
Antibacterial And Antifungal

The cow is considered a divine animal in the Hindu culture. The milk, cow dung, urine of the cow is used for various purposes. The benefits of cow urine have been depicted in ancient Hindu texts, Vedas. The present study aimed at using cow urine as a plant growth enhancer and antimicrobial agent. The plants chosen for this study were Zea mays (maize), Triticum aestivum (wheat),Cymbopogon citratum (grass), Ocimum basilicum (tukmaria) and Trigonella foenum graecum (methi). The plants were grown for 30 days using different cow urine concentrations i.e 0%(control), 5%, 15%, 25%. The various parameters such as plant height, shoot, and root length, number of leaves, the mass of the root etc were observed. The antibacterial test using different cow urine concentrations i.e 0%(control), 5%, 15%, 25% was conducted on Escherichia coli using disc diffusion method. The fungus was screened and isolated from raw coconut and was grown on YPD media to obtain the mother culture. Using Slide culture technique, the fungi were identified as Aspergillus. The media was poisoned using different cow urine concentrations i.e 0%(control), 5%, 15%, 25% and the fungi culture was inoculated. GCMS analysis was conducted to identify the compounds present in the cow urine. Among the concentrations, 5% cow urine concentration showed maximum growth when compared to other concentrations whereas 25% concentration showed more antibacterial and antifungal activity when compared to others. In GCMS Analysis, 16 compounds have been identified, in which, 6 compounds were antifungal, 3 compounds were antibacterial, and 2 compounds as plant growth enhancers. This study concludes that cow urine can be used as a plant growth enhancer and it possesses antimicrobial characteristics.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals ANTIFUNGAL ACTIVITY OF RED GALANGAL (Alpinia purpurata K. Schum) ETHANOL EXTRACT AGAINST Malassezia furfur AND Microsporum canis

2020 ◽  
Vol 3 (2) ◽  
pp. 76-81
Author(s):  
Rini Lestari
Keyword(s):  
Antifungal Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Average Concentration ◽  
Diffusion Method ◽  
Microsporum Canis ◽  
Malassezia Furfur ◽  
Agar Diffusion Method ◽  
Alpinia Purpurata ◽  
Anti Fungal

Red galangal (Alpinia purpurata) is a plant that belongs to the Zingiberaceae family. Red galangal rhizome is traditionally used to treat phlegm and ringworm. Red galangal rhizome contains secondary metabolites of essential oils, eugnol, sesquiterpenes, pinen, kaemferida, galanagn and galangol which have anti-fungal properties. This study aims to determine the antifungal activity of red galangal rhizome ethanol extract against Malasezia furfur and Microsporum canis using agar diffusion method. Red galangal extract is made with a concentration variation of 2%, 5% and 10%. The results showed the antifungal activity of red galangal ethanol extract in the form of inhibiting diameters at an average concentration of 2%, 5% and 10% against Malasezia furfur was 16.77 mm, 17.91 mm and 19.72 mm. The average inhibitory diameter of Microsporum canis is 10.58 mm, 13.57 mm and 14.51 mm. Based on the results of this study it can be concluded that the ethanol extract of red galangal has antifungal activity against Malasezia furfur and Microsporum canis.

scholarly journals Antagonistic Potentiality of Some Soil Fungi Against Six Fungal Pathogens Isolated From Cotton (Gossypium Hirsutum L.) Seeds

2021 ◽  
Vol 46 (2) ◽  
pp. 143-153
Author(s):  
Amina Khatun ◽  
Shamim Shamsi ◽  
Abul Bashar
Keyword(s):  
Gossypium Hirsutum ◽  
Growth Inhibition ◽  
Radial Growth ◽  
Fungal Pathogens ◽  
Soil Fungi ◽  
The Other ◽  
Dual Culture ◽  
Gossypium Hirsutum L ◽  
Volatile Metabolites ◽  
Other Hand

The antagonistic potentials of four soil filamentous fungi viz., Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tieghem and Trichoderma viride Pers. against six pathogens isolated from 14 varieties of cotton (Gossypium hirsutum L.) were evaluated by "dual culture colony interaction”, volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride was found be the most effective to control the growth of the cotton pathogens. T. viride showed the highest growth inhibition against Curvularia lunata, Fusarium moniliforme, Mucor sp. and Rhizoctonia solani. On the other hand A. niger showed the highest growth inhibition against Fusarium nivale and A. fumigatus showed the maximum growth inhibition against C. gloeosporioides. The highest inhibition of radial growth of C. lunata, F. nivale and F. moniliforme was observed might be due to the secretion of volatile metabolites of T. viride whereas, the maximum inhibition of radial growth of C. gloeosporioides was observed because of the volatile metabolites of A. flavus. Mucor sp. and R. solani were inhibited due to the volatile metabolites of A. fumigatus. The greatest radial growth inhibition of C. lunata and F. moniliforme were noticed in case of T. viride owing to the effect of non-volatile metabolites. On the other hand, the radial growth of C. gloeosporioides and F. nivale were inhibited highest amount for the effect of non-volatile metabolites of A. niger, whereas highest growth inhibition of Mucor sp. and R. solani was observed due to the non-volatile effect of A. fumigatus. The present investigation suggests that the isolates of Aspergillus and Trichoderma may be further exploited as potential biocontrol agents against the fungal pathogens of cotton in field trial. Asiat. Soc. Bangladesh, Sci. 46(2): 143-153, December 2020

scholarly journals 548 Antitranspirant Treatments Affect Stock Plant Growth and Rooting Percentage in Two Members of the Oleaceae Family

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 490B-490
Author(s):  
Susan Switras ◽  
Jeffrey Gillman
Keyword(s):  
Plant Growth ◽  
Sodium Silicate ◽  
Shoot Length ◽  
The Other ◽  
Stock Plant ◽  
Film Forming ◽  
Length Changes

Syringa vulgaris L. `Montaigne' (French hybrid lilac) and Chionanthus virginicus L. (white fringetree) stock plants were sprayed with two film-forming antitranspirants, Clear Spray and sodium silicate, and one stomate-regulating antitranspirant, atrazine. After 30 days of growth, cuttings were taken from both species. Shoot calipers of antitranspirant-treated lilacs increased at a slower rate than the control lilacs. There were no long-term shoot length changes in lilac due to antitranspirant treatments. Shoots of Clear Spray-treated fringetrees increased in length significantly less than other treatments. Rooting was evaluated once a week from week 3 until 6 weeks after cuttings were taken for lilac and every 2 weeks from week 6 to 12 weeks after sticking for fringetree. All treatments resulted in 80% to 85% rooting in lilac after 6 weeks, but Clear Spray-treated plants rooted more quickly. Fringetree cuttings treated with Clear Spray had a significantly lower rooting percentage (15%) than the other treatments (35% to 50%).

scholarly journals First Report of Rhizoctonia solani AG-7 on Cotton in Mississippi

Plant Disease ◽  
2000 ◽  
Vol 84 (10) ◽  
pp. 1156-1156
Author(s):  
R. Baird ◽  
W. Batson ◽  
D. Carling ◽  
M. Scruggs
Keyword(s):  
Plant Growth ◽  
Gossypium Hirsutum ◽  
Rhizoctonia Solani ◽  
Field Study ◽  
Potato Dextrose Agar ◽  
Distilled Water ◽  
Gossypium Hirsutum L ◽  
First Report ◽  
Secondary Roots ◽  
Isolate Identification

During a field study of the soilborne mycobiota on cotton (Gossypium hirsutum L.) roots, Rhizoctonia solani Kühn AG-7 was isolated from dark brown lesions present on the tap, feeder, and secondary roots onto potato-dextrose agar (PDA) (34 g of medium per liter of distilled water). Isolate identification was confirmed using tester R. solani AG-7 isolates (Carling, University of Alaska) for comparison during anastomosis pairing. To confirm pathogenicity, six AG-7 isolates (65, 66, 67, 68, 69, 70) obtained from cotton roots were tested in a plant growth incubator (18–24°C) by mixing 2.5 ml of 2-week-old cornmeal sand inoculum (3 g cornmeal, 100 g sand, and 20 ml distilled water) with 500 ml of autoclaved soil into each of five replicate polystyrene pots (15 cm wide × 20 cm long) per isolate. Five control pots containing noninfested soil were added for comparison. Five seed of cv. Deltapine 50 were sown into each pot. For all six isolates, mean stand counts (ranging from 0 and 1.3 plants per pot) were significantly lower (P ≤ 0.05) compared with those of plants in noninfested pots (3.8 plants per pot) at 21 days after planting. Furthermore, when the roots of surviving seedlings were evaluated for disease severity, brown, discolored lesions were observed at the base of stems and on roots in infested pots for all six isolates. Six AG-7 cultures of the pathogen were reisolated from symptomatic tissues onto PDA. The experiment was repeated with similar results. This is the first report of AG-7 occurring in Mississippi.

Antibacterial activity of several Indonesian plant extracts and combination of antibiotics with Syzygium malaccense extract as the most active substance

2020 ◽  
Vol 11 (3) ◽  
pp. 3300-3308
Author(s):  
Yuniarni U ◽  
Sukandar E Y ◽  
Fidrianny I
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
The Other ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Vaginal Infections ◽  
Gram Negative ◽  
Nephelium Lappaceum ◽  
Infection Disease ◽  
Agar Diffusion Method

Natural products have an important role in the development of drugs, especially as an antibacterial. Some plant extracts in Indonesia such as Syzygium (Myrtaceae) and Graptophyllum (Acanthaceae) have been used empirically for the treatment of non-infectious diseases, and are also for management infections such as throat, mouth and vaginal infections, dysentery and diarrhea. The objectives of this study are to investigate antibacterial activity of 11 extracts from ten plants which were used in traditional medicine in Indonesia to treat infection disease and to evaluate interaction between potential antibacterial extract and antibiotics. Antibacterial activity and combination extract with antibiotics were evaluated using agar diffusion method on Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. Among the ten plants, Syzygium malaccense leaves extract, and Nephelium lappaceum peel and leaves extracts showed significant antibacterial activity. They have antibacterial activity against Gram positive and Gram negative. S. malaccense extract had better antibacterial activity against P. aeruginosa compared to the other tested bacteria and provided better activity compared to N. lappaceum leaves extract. Phytochemical screening showed that S. malaccense leaves extract contained flavonoid, tannin, quinone, phenol and triterpenoid/steroid. The combination of S. malaccense extract with antibiotics showed that the usage of S. malaccense extract together with ciprofloxacin gave antagonistic result, so the usage of S. malaccense extract together with ciprofloxacin needs caution because it can reduce the effect of ciprofloxacin.

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