scholarly journals Selection of suitable media and intervals of media inoculation for culturing Tubificid worms

2013 ◽  
Vol 10 (2) ◽  
pp. 325-330
Author(s):  
MFA Mollah ◽  
K Mosharaf ◽  
Dr Mariom
Keyword(s):  
Culture Media ◽  
Fine Sand ◽  
Mustard Oil ◽  
Cow Dung ◽  
Running Water ◽  
Mustard Oil Cake ◽  
Culture Duration ◽  
The Media ◽  
Tubificid Worms ◽  
Selection Of

Tubificid worms are aquatic invertebrates, belonging to the class Oligochaeta and family Tubificidae, used as an important live food for fishes. The study was conducted to culture Tubificid worms under running water in order to develop a suitable culture media and an optimum duration of media inoculation for culturing Tubificid worms. The worms were cultured under two experiments in cemented culvert system (160×25×10 cm3) for 90 days. In the first experiment the worms were cultured in three different media designated as treatment-I, treatment-II and treatment-III. The highest yield (503.39±22.98 mg cm-2) was found at 70th day of culture duration in the culture media containing a mixture of 35% mustard oil cake, 20% wheat bran, 25% cow-dung and 20% fine sand (treatment-III). Only 1.99 kg media ingredients valued BDT 29.85 were needed to yield 1 kg worms. In the second experiment, the worms were cultured at three different intervals of media inoculation i.e., 6, 10 and 15 days interval designated as treatment-I, treatment-II and treatment-III respectively using the media found best in the first experiment. Inoculation of media at 10 days interval showed significantly (P<0.01) higher production (488.94±5.60 mg cm-2).DOI: http://dx.doi.org/10.3329/jbau.v10i2.14925 J. Bangladesh Agril. Univ. 10(2): 325-330, 2012

scholarly journals Optimum harvest for sustainable yield of fish live food tubificid worms

1970 ◽  
Vol 20 (1) ◽  
pp. 57-63
Author(s):  
Anwar Hossain ◽  
Mohammad Shamsur Rahman ◽  
Mahmud Hasan
Keyword(s):  
Wheat Bran ◽  
Maximum Yield ◽  
Live Food ◽  
Mustard Oil ◽  
Cow Dung ◽  
Sustainable Yield ◽  
Fish Yield ◽  
Mustard Oil Cake ◽  
The Media ◽  
Tubificid Worms

The Optimum harvest suitable for sustainable yield of tubificid worms without affecting the future yield for a period of 60 days was determined. The media ingredients used to culture the worms were 20% wheat bran, 30% soybean meal, 20% mustard oil cake, 20% cow dung and 10% sand. A harvest level of 50 mg/cm2 (maximum yield; 518.93 ± 14.36 mg/cm2) at ten days interval starting from 30 days of worms' inoculation was found suitable for sustainable yield in the later sampling durations. Results of this study have implications in increasing fish yield by producing reliable and sustainable yield of fish live foods tubificid worms.Key words: Tubificid worms; Live food; Optimum harvest; Sustainable yieldDOI: http://dx.doi.org/10.3329/dujbs.v20i1.8838Dhaka Univ. J. Biol. Sci. 20(1): 57-63, 2011 (January)

scholarly journals Effects of Wetting Media Cattle Blood, Rice Gruel and Water on the Yield and Amino Acid Composition of Tubificid Worms

2019 ◽  
Vol 45 (2) ◽  
pp. 229-239
Author(s):  
Mahmud Hasan ◽  
Md Inja Mamun Haque ◽  
Nafia Akter ◽  
Mohammad Shamsur Rahman ◽  
Afroza Sultana Eti
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Soybean Meal ◽  
Mustard Oil ◽  
Fish Food ◽  
Mustard Oil Cake ◽  
Cattle Blood ◽  
Tubificid Worms

The effects of three wetting media (cattle blood, rice gruel and subsurface clean water) of the culture media ingredients (a mixture of mustard oil cake, soybean meal and mud) on the yield and amino acid composition of tubificid worms cultured on a pilot scale in a fish farm for 42 days were evaluated. The worm yields were in the order: cattle blood wetted media (683.68 ± 3.86 mg/cm2), rice gruel (615.63 ± 3.66 mg/cm2), water (584. 38 ± 1.41 mg/cm2). Lysine, arginine and leucine were the most prevalent of the eight essential amino acids in worms cultured in the media wetted in cattle blood medium. The results suggested that a mixture of 40% mustard oil cake, 30% soybean meal and 30% mud can be used for the commercial culture of tubificid worms as live fish food. The tubificid worms showed high level of amino acids suitable for fish that require a high content of dietary amino acids. Asiat. Soc. Bangladesh, Sci. 45(2): 229-239, December 2019

scholarly journals Impacts of Northwest Fisheries Extension Project (NFEP) on pond fish farming in improving livelihood approach

1970 ◽  
Vol 8 (2) ◽  
pp. 305-311
Author(s):  
MR Islam ◽  
MR Haque
Keyword(s):  
Group Discussion ◽  
Stocking Density ◽  
Fish Farming ◽  
Mustard Oil ◽  
Cow Dung ◽  
Fish Production ◽  
Government Officials ◽  
Mustard Oil Cake ◽  
Phase Out ◽  
Organic And Inorganic Fertilizers

Investigation was carried out from June to August 2009. A total of 40 fish farmers were selected from northwest two upazila namely Debigonj (n=20) and Boda (n=20) where both men and women were targeted. Focus group discussion (FGD) and cross-check interview were conducted to get an overview on carp farming. From 1991-1995, 1996-2000 and after 2000; 17.5%, 45% and 37.5% of fish farmers started carp farming respectively. Average 77.5% of farmers acquired training from NFEP project while 10% of them from government officials. There were 55% seasonal and 45% perennial ponds with average pond size 0.09 ha. After phase out of NFEP project, 92.5% of fish farmers followed polyculture systems, while only 7.5% of them followed monoculture ones. Farmers did not use any lime, organic and inorganic fertilizers in their ponds before association with NFEP project. They used lime, cow dung, urea and T.S.P during pond preparation at the rate of 247, 2562.68, 46.36 and 27.29 kg.ha-1.y-1 respectively where stocking density at the rate of 10,775 fry.ha-1 after phase out of the project. Feeding was at the rate of 3-5% body weight.fish-1.day-1. Rice bran, wheat bran and mustard oil cake used at the rate of 1920, 220 and 110 kg.ha-1.y-1 respectively by 85% of fish farmers but 15% of them did not used any feed. Before NFEP project, average pond production was 408.55 kg.ha-1 but after phase out the project, fish production was 2,861.08 kg.ha-1. Peak culture and harvesting period lied from April to December and from November to January. Average 92.5% of farmers improved their livelihood through carp farming. Fish farming was affected due to low water depth and drought conditions. Keywords: NFEP; Fish farming parameters; Pond production; Livelihood approach DOI: 10.3329/jbau.v8i2.7942 J. Bangladesh Agril. Univ. 8(2): 305-311, 2010

scholarly journals Fish Production Technology of Small Scale Fish Farmers at Chaugachha Upazila under Jashore District of Bangladesh

2020 ◽  
pp. 1-6
Author(s):  
Md. Rasal Ali ◽  
B.M. Newaz Sharif
Keyword(s):  
Organic Fertilizer ◽  
Stocking Density ◽  
Fish Farming ◽  
Mustard Oil ◽  
Small Scale ◽  
Cow Dung ◽  
Just Culture ◽  
Mustard Oil Cake ◽  
Supplementary Feed ◽  
Rural Appraisal

The study was conducted on the fish farmer, which was situated at Chaugachha Upazila, Jashore, from October 2015 to April 2016. Data were collected using participatory rural appraisal (PRA) tools and personal observation. About 62% of the farmers have ponds of single, and 38% have multiple ownership. The homestead and commercial ponds were 79% and 21%, respectively. About 100% farmer carried out poly-culture fish farming though they did not know poly-culture just culture of various fishes. About 98% of the farmers control aquatic weeds manually. For controlling undesirable species, most of them (95.74%) used the netting method. Liming used 185. 3-247 kg/ha and organic fertilizer, mainly cow dung used 741-1235 kg/ha. Average stocking density was found to be 12326 fry/ha. 91.5% of the farmers applied supplementary feed, such as both rice-bran and mustard oil-cake. The peak harvesting period was found from December to January. In this season, around 65% of the stocked fishes were reported to harvest, and the rest of the fish (35%) was harvested during another season.

scholarly journals Soil Test Based Inorganic Fertilizer and Integrated Plant Nutrition System for Rice (Oryza sativa L.) Cultivation in Inceptisols of Bangladesh

2016 ◽  
Vol 14 (1) ◽  
pp. 33-42 ◽  
Author(s):  
M I Ullah Sarkar ◽  
M M Rahman ◽  
G K M M Rahman ◽  
U A Naher ◽  
M N Ahmed
Keyword(s):  
Oryza Sativa ◽  
Plant Nutrition ◽  
Poultry Manure ◽  
Inorganic Fertilizer ◽  
Yield Potential ◽  
Mustard Oil ◽  
Soil Test ◽  
Cow Dung ◽  
S Uptake ◽  
Mustard Oil Cake

A field experiment was conducted at the Bangladesh Rice Research Institute, Gazipur during 2013 to study the potential of different organic materials in integrated plant nutrition system (IPNS) and soil test based (STB) inorganic fertilizer on the growth, nutrient uptake and yield of rice (<i>Oryza sativa</i> L.): variety BRRI dhan29. Five treatments were laid out in a randomized complete block design with four replications. The treatments were: (1) soil test based inorganic fertilizer (NPKS @ 138-7-80-3 kg ha-1), (2) cow dung (3 t ha-1), (3) poultry manure (2 t ha-1), (4) mustard oil cake (2 t ha-1) and (5) control. Organic fertilizers were applied on dry weight basis. Inorganic fertilizers were applied along with poultry manure (PM), cow dung (CD) and mustard oil cake (MOC) as IPNS. Soil test based STB fertilizer and PM in IPNS provided maximum grain yield of rice (6.11 t ha-1). Maximum plant height and tiller numbers were found in MOC treatment, while the numbers of filled grains were higher in STB fertilizer and PM treated plots. The application of different organic sources as IPNS significantly influenced the N, P, K and S uptake in rice. However, the highest N, P, K and S uptake were found in the PM treatment. The benefit cost ratio of different treatments followed the order of STB>PM>CD>Control>MOC. Considering yield potential and BCR, STB fertilizer and PM treatments were found economically profitable. However, from environmental point of view and sustainable management of soil health and crop productivity, application of PM and CD in rice-rice cropping system is recommended for rice cultivation.The Agriculturists 2016; 14(1) 33-42

scholarly journals The Roles of Porous Coral Sands in Initial Enrichment of Ammonia-Oxidizing Bacteria

2007 ◽  
Vol 7 ◽  
pp. 525-532
Author(s):  
Qing Guo ◽  
Zao-he Wu ◽  
Ming-liang Qian ◽  
Binhe Gu
Keyword(s):  
Culture Media ◽  
Potassium Dihydrogen Phosphate ◽  
N:P Ratio ◽  
Ammonium Nitrogen ◽  
Dihydrogen Phosphate ◽  
Ammonia Oxidizing Bacteria ◽  
Potassium Dihydrogen ◽  
Conversion Rates ◽  
Calcium Source ◽  
The Media

The purpose of this study was to investigate the roles of coral sands in the enrichment and isolation of ammonium-oxidizing bacteria (AOB). We hypothesized that the porous coral sands provided additional surface area and nutrients for the growth of periphytic AOB. In the present study, an orthogonal test was designed to compare the AOB conversion rates of ammonium-nitrogen (NH4+N) to nitrite-nitrogen (NO2--N) among various combinations of culture media. Results showed that the conversion of NH4+N to NO2--N increased significantly when the coral sands were added, implying that coral sands were beneficial to the growth of AOB. Additions of potassium dihydrogen phosphate (KH2PO4) or sodium bicarbonate (NaHCO3) to the media became unnecessary when coral sands were used, but the addition of KH2PO4was needed when the molar nitrogen to phosphorus (N:P) ratio reached 10 in the enrichment media using calcium carbonate (CaCO3) powder as a calcium source.

Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos

2021 ◽  
Author(s):  
Gabriela de Oliveira Fernandes ◽  
Marcella Pecora Milazzotto ◽  
Andrei Antonioni Guedes Fidelis ◽  
Taynan Stonoga Kawamoto ◽  
Ligiane de Oliveira Leme ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biochemical Markers ◽  
Culture Medium ◽  
Culture Media ◽  
Ionization Mass ◽  
Metabolic Profiles ◽  
Bovine Embryos ◽  
The Media

Abstract The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recipients. After pregnancy diagnosis, the media were grouped into the pregnant and nonpregnant groups. The metabolic profiles of the media were analyzed via electrospray ionization mass spectrometry, and the concentrations of pyruvate, lactate, and glutamate were assessed using fluorimetry. The spectrometric profile revealed that the media from embryos from the pregnant group presented a higher signal intensity compared to that of the nonpregnant group; the ions 156.13 Da [M + H]+, 444.33 Da [M + H]+, and 305.97 Da [M + H]+ were identified as biomarkers. Spent culture medium from expanded blastocysts (Bx) that established pregnancy had a greater concentration of pyruvate (p = 0.0174) and lesser concentration of lactate (p = 0.042) than spent culture medium from Bx that did not establish pregnancy. Moreover, pyruvate in the culture media of Bx can predict pregnancy with 90.9% sensitivity and 75% specificity. In conclusion, we identified markers in the culture media that helped in assessing the most viable IVP embryos with a greater potential to establish pregnancy.

Assessment of rotary drum and aerated in-vessel for composting of garden waste

2021 ◽  
Author(s):  
Sandeep Mishra ◽  
Kunwar D Yadav
Keyword(s):  
Cow Dung ◽  
Blade Design ◽  
Percentage Reduction ◽  
Acid Soluble Lignin ◽  
Odour Control ◽  
Rotary Drum ◽  
Working Performance ◽  
Soluble Lignin ◽  
Selection Of ◽  
Garden Waste

Abstract For in-vessel composting of garden waste, the selection of reactor is an important factor for efficient degradation. The present study evaluates working performance of rotary drum reactor (RDR) and aerated in-vessel (AIV) for composting of garden waste. 100 kg garden waste was mixed with 10 kg cow-dung slurry and 5 kg compost and feed into both the reactors for 45 days composting period. The reactors vary in their system configuration, shape and orientation, blade design, rate of aeration, odour control, leachate production and energy requirements. Rotary drum was rotated daily six times in clockwise and anti-clockwise direction and AIV was rotated daily for 3–5 minutes using motor. Rise in temperature started within 24 hours of composting and reached 65°C and 59°C on second day itself and thermophilic phase continued for 7 and 5 days for RDR and AIV respectively. Moisture content reduction after composting period was 15.25 and 18.45 %, C/N ratio was 16.14 and 13.33, TVS reduction was 23.74 and 29.78 % and CO2 evolution rate was 6.18 and 4.14 mg/g VS/day in RDR and AIV respectively. Reduction of hemicellulose, cellulose, and lignin was more in AIV. The percentage reduction of acid insoluble lignin was 36.10 and 29.01 % and the percentage reduction of acid soluble lignin was 48.85 and 43.3% in in AIV and RDR respectively after 45 days. AIV gave better performance for composting of garden waste.

scholarly journals The Role of Sequestrene 138 in Highbush Blueberry (Vaccinium corymbosum L.) Micropropagation

HortScience ◽  
2018 ◽  
Vol 53 (10) ◽  
pp. 1487-1493 ◽  
Author(s):  
Doina Clapa ◽  
Claudiu Bunea ◽  
Orsolya Borsai ◽  
Adela Pintea ◽  
Monica Hârța ◽  
...  
Keyword(s):  
Culture Media ◽  
Iron Concentration ◽  
Vaccinium Corymbosum ◽  
Carotenoid Content ◽  
Free Medium ◽  
Axillary Shoots ◽  
Different Types ◽  
The Media ◽  
Dark Green

The current research was carried out to investigate the effects of iron source in the culture media for Vaccinium corymbosum L. ʻBluerayʼ, ʻDukeʼ, and ʻPatriotʼ cultivars grown on five different types of medium (Woody Plant Medium supplemented with 1.0 mg·L−1 zeatin and 0, 25, 50, 75, and 100 mg·L−1 Sequestrene 138). After 10 weeks of culture, seven physiological parameters were measured, such as the number and length of axillary shoots, rooting and acclimatization percentage, as well as chlorophyll (a, b, a/b) and carotenoid content of the leaves. Adding Sequestrene 138 to the culture media led to a slight decrease of the proliferation rate but increased the length of the shoots. The chlorophyll and carotenoid content in all of the three cultivars was considerably increased as the iron concentration of the media increased. The shoots developed on the Sequestrene 138–free medium were chlorotic and short, whereas at different concentrations of iron in the culture medium the shoots were dark green and vigorous, providing a greater acclimatization success than those grown in iron-free medium.

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