scholarly journals Microbial quality of hilsa shad (Tenualosa ilisha) at different stages of processing

2012 ◽  
Vol 9 (2) ◽  
pp. 339-344 ◽  
Author(s):  
MM Shamsuzzaman ◽  
SK Mazumder ◽  
MA Siddique ◽  
MNU Miah

An attempt was made from October 2008 to March 2009 in a fish processing industry of Bangladesh (the Sea    Resource Limited, Sadarghat, Chittagong) to determine microbial quality of Tenualosa ilisha at different stages of    processing. During this investigation Escherichia coli, fecal coliform, vibrio cholerae, Salmonella and total load of    bacteria were identified from 5 stages of processing. Those stages were receiving, primary washing stage with 5PPM  chlorine water, final washing stage with 10 PPM chlorine water, after blast freezing at -40 ± 20C and finally after one    month storage at -18 ± 20,/sup>C. It was observed that 80.69 % of total bacterial load, 77.29% of total coliform and 58.33 %    of fecal coliform were destroyed during different processing stages. There was no evidence of presence of    Salmonella and Vibrio cholera at any stages of processing. DOI: http://dx.doi.org/10.3329/jbau.v9i2.11049 J. Bangladesh Agril. Univ. 9(2): 339–344, 2011

Author(s):  
Ibraheem Ismail Shabbaje Ibraheem Ismail Shabbaje

This research was conducted to follow up the microbial contamination samples of some seasoning salad samples collected twice monthly for three months from twelve locations representing six districts in Jeddah. It was found that the counts of microbial groups (total viable bacteria, total coliform, fecal coliform, Salmonella and Staphylococcus) in the sample taken at rush hours were higher than those collected at calm time of selling. Also the highest means of all studied microbial groups were found in the samples taken from traditional locations at calm time of selling. The highest mean of total viable bacterial count (169600 cfu/gm) was found in the samples of Mashrafa District in the first sampling month. Whereas, the highest means of total coliform group (80866 cfu/gm) and fecal coliform group (28633 cfu/gm) were found in the samples taken from traditional locations in Guesa at the first sampling month and in Al-Rwaubi district at the second sampling month, respectively. On the other hand, Samples' means of from traditional locations taken from Al-Rehab district at the third month of sampling (35500 cfu/gm) and from the same district at the second sampling month (25000 cfu/gm) represented Salmonella and staphylococcus groups, respectively. It is recommended to take care about purchasing of ready to eat food and the direct eating or cold preservation until using to avoid food infection or poisoning.


Author(s):  
Nesrin Kaval ◽  
Nilgün Öncül ◽  
Zeliha Yıldırım

In this study, the microbiological quality of traditionally produced Bez Sucuk in Tokat and its vicinity was investigated. For this purpose, 30 Bez Sucuk samples obtained from butchers, producers of Bez Sucuk, were analyzed for total count of mesophilic aerobic bacteria, lactic acid bacteria, yeasts-moulds, total coliform, fecal coliform, Staphylococcus aureus, Bacillus cereus, and Cl. perfringens. Also, the presence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. were investigated in all samples. pH and water activity of the samples were determined. As the result of the analysis, the counts of the microbiological parameters investigated were found as follows: total mesophilic aerobic bacteria 3.5×106-4.23×109 CFU/g, lactic acid bacteria 5.55×105-2.45×109 CFU/g, yeasts and moulds 2.50×103-6.90×109 CFU/g, total coliform


Author(s):  
Anil Gill ◽  
Akash John ◽  
Neelam Iqbal ◽  
Tallat Anwar Faridi ◽  
Sofia Noor

Bakery items are a vital element of a well-balanced diet, and supermarket shelves now stock a wide range of them. Bakery items are the most popular ready-to-eat foods (REF).Objective:To assess the microbial quality of bakery foods (Bread, Cake and patties) and check the total plate count (TPC), bacterial and yeast count Methods:The analysis of the microbial quality of bakery products was determined for three different bakery products (bread, cake and patties) at ten different locations collected from a renowned bakery in Lahore, Pakistan. The samples were assessed under complete hygienic conditions by standardized plate count (TPC) and MPN method. Micro-organisms (Coliform, Fecal Coliform and Yeast) were lately confirmed by their morphological characteristics and biochemical tests Results: Among the three commodities, bread was found to be highly contaminated by micro-organisms and the total plate count recorded was 4.7 x 105cfu g-1collected from sector I9 which is not consumable. Whereas Patties showed the number of TPC as 1.6 x 105 cfu g-1also collected from sector I9. Cake was revealed as least infected bakery product i.e., 5.7 x 104 cfu g-1. The above results showed that sector I9 is highly contaminated and a threat for health. Bacterial plate count (Coliform and Fecal Coliform count) was highly measured in bread as 460 MPN g-1 taken from sector I5 where as it remains under the permissible limit determined by WHO, 1994 in remaining sectors Conclusions:In conclusion the bakery food is contaminated by the use of un-hygienic water, miss handled during the process after baking i.e., slicing, serving and packaging. To avoid a possible epidemic of food borne illness, competent authorities should conduct a stricter and more stringent inspection of  REFs sold to clients in bakeries.


Agriculture ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 18
Author(s):  
Joycelyn Quansah ◽  
Cesar Escalante ◽  
Angela Kunadu ◽  
Firibu Saalia ◽  
Jinru Chen

Vegetable farming is the most practiced urban agriculture in Ghana. A previous study of our laboratory revealed poor microbial quality of, and presence of Salmonella on, leafy green vegetables grown or sold in Accra, Ghana. The aims of this study were to determine agricultural practices of urban vegetable farmers and the association between agricultural practices and microbial safety of vegetables produced. A survey was conducted among 102 farmers from 12 farming areas who produced exotic and indigenous leafy greens in Accra, Ghana to identify their farming practices. It was observed that water from waste drains pumped into shallow wells was used for irrigation by 70% of the farmers. Incompletely composted manure was commonly used (99%) in farming. Vegetables were usually harvested using bare hands (96%) and knives (73%) and transported mainly in sacks (94%) to market centers under non-refrigerated conditions. Over 60% of the farmers disagreed that the use of polluted irrigation water can contaminate vegetables or make consumers sick. According to the seemingly unrelated regression model, farmers with no formal education and less food safety knowledge and had been planting on their current farmlands for several years were likely to produced vegetables with higher fecal coliform and Enterococcus sp. counts compared to other farmers. Vegetables cultivated by farmers who disagreed that the use of contaminated water can make consumers sick were associated with the production of vegetables with high fecal coliform and Enterococcus sp. counts. Education and improved agricultural and post-harvest handling practices should be encouraged among vegetable producers in the area to improve food safety.


1970 ◽  
Vol 24 (2) ◽  
pp. 160-162 ◽  
Author(s):  
Meshkatul Jannat ◽  
Nazmul Ahsan ◽  
M Mon Islam ◽  
M Raknuzzaman ◽  
M Niamul Naser ◽  
...  

In this study, bacterial load of Hilsha (Tenualosa ilisha) collected from different catching points, open market and department shop was determined. Samples collected from catching points contained considerably more bacteria than those collected from open market or department shop. This result indicated that fish traders might use some preservatives that reduced the bacterial load. Besides, fish from department shop contained more bacteria than fishes from open market. The possible reason for this variation might be due to fact that the unsold fishes were kept longer times in the department shops. Among the different parts of fish body, maximum number of bacteria was found in scale and skin followed by gill, gut and muscle. High number of coliform bacteria was also found in the samples tested. Proper handling and precautions is important for maintaining the quality of Hilsha.Keywords: Bacterial load, Hilsha (Tenualosa ilisha), Coliform, Faecal coliformDOI: http://dx.doi.org/10.3329/bjm.v24i2.1265


2017 ◽  
Vol 14 (2) ◽  
pp. 587-591
Author(s):  
K. Mchiouer ◽  
S. Bennani ◽  
N. Sh. El-Gendy ◽  
M. Meziane

ABSTRACT: The aim of this study is to determine the microbiological quality of raw cows’ milk of Oujda city. Raw milk samples are collected randomly between June 2014 and May 2015 from 20 Mahlaba (dairies) for microbiological evaluation. The samples are analyzed to determine total mesophilic aerobic bacteria (TMAB), total coliform, fecal coliform, staphylococcus aureus, fecal streptococci, proteolytic bacteria and lactic acid bacteria. The results of bacterial count showed that there is a variation between all the milk samples and a period effect is also observed. The mean counts of total mesophilic aerobic bacteria from all sale points are between 1.76×106 and 40.17×106 CFU/ml. Milk samples reveled counts total coliform and fecal coliform ranging from 0.58×105 to 11.10×105 CFU/ml and from 0.60×103 to 14.64×103 CFU/ml, respectively. Staphylococcus aureus are also detected in all samples with counts ranging from 0.35 ×103 to 3.08×103 CFU/ml. Fecal streptococci are found in all milk samples, at counts ranging from 0.16 ×102 to 2.18 ×102 CFU/ml. And finally, proteolytic and lactic acid bacteria are between 0.3×103 and 2.86×103 CFU/ml and 2.37×106 and 24.14 ×106 CFU/ml respectively. These results indicate a lack of compliance with good manufacturing practice at milking, collection and transportation of raw milk.


2014 ◽  
Vol 12 (2) ◽  
pp. 231-236
Author(s):  
R Khaton ◽  
MA Hasnat ◽  
S Rahman ◽  
MM Rahman

The aim of the present study was to determine the microbial quality of freshly drawn cow’s milk belonging to the arsenic affected and non-affected areas. In the present work 25 milk sample were collected from five different arsenic affected and non-affected areas. The analysis comprised enumeration of total viable count (TVC), total coliform count (TCC) and total staphylococcal count (TSC) for the determination of sanitary quality. The highest total viable count, total coliform count and total staphylococcus count were log 5.894 ± 0.221, log 2.832± 0.129 and log 2.898 ± 0.162 respectively. Staphylococcus spp, Escherichia coli, Pseudomonas spp and Bacillus spp were isolated from the milk samples. Among them Escherichia coli were isolated from the maximum milk samples. It is found that the level of microbial quality in terms of TVC, TCC and TSC were high in arsenic affected areas than arsenic non-affected area. It also found that TVC and TCC were positively correlated with each other. This survey indicates that most of the raw milk samples were not satisfactory in course of public health standard as some pathogenic bacteria were detected from these samples and it is necessary to improve the hygienic practices required for the handling and processing of milk.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21297 Bangl. J. Vet. Med. (2014). 12 (2): 231-236 


1992 ◽  
Vol 55 (2) ◽  
pp. 104-107
Author(s):  
DEOGHWAN OH ◽  
DOUGLAS L. MARSHALL ◽  
MICHAEL W. MOODY ◽  
J. DAVID BANKSTON

Microbiological analyses were made on samples of cooked blue crab taken immediately after debacking and either forced-air cooling or static-air cooling. Forced-air cooling had significantly lower (P<0.05) total coliform and fecal coliform counts, 2.51 and 2.30 log10 MPN/100 g, compared with those of static-air cooling, 2.83 and 2.60 log10 MPN/100 g. All treatments had less than 2.30 log10 MPN/100 g Escherichia coli. Staphylococcus aureus counts in the forced-air cooled crabs were approximately 4-fold lower than counts in static-air cooled crabs. The aerobic plate counts and psychrotrophic plate counts were significantly lower (P<0.01) by 1.04 and 0.81 log10 CFU/g, respectively, by forced-air cooling compared to static-air cooling. Thermocouple temperature readings were used to determine differences in cooling rates between forced-air and static-air cooling. After 1.5 h of cooling, the initial precooled crabmeat temperature of 34°C (93°F) was reduced by forced-air cooling and static-air cooling to 4°C (40°F) and 20°C (67°F), respectively. The rates of cooling using forced-air and static-air were significantly different (P<0.01).


2011 ◽  
Vol 63 (6) ◽  
pp. 1255-1264 ◽  
Author(s):  
K. Patel ◽  
A. K. Mungray

Performance of the combined process of up-flow anaerobic sludge blanket (UASB) reactor and cascade sponge reactor (CSR) for sewage treatment was studied. UASB-CSR system was operated at HRTs of 24 h, 16 h, and 8 h at an average wastewater temperature of 29°C. It comprises of the most efficient combined process not only for CODT (98.9%), BODT (98.5%), TSS (99.3%), total nitrogen (89.1%), total phosphorus (99.0%), total coliform (99.9%) and fecal coliform (99.9%) removal but also for reducing excess sludge production. Fecal coliform counts were found 23 MPN/100 ml only in final effluents. The effluent quality of the system sufficiently meets the discharged standards which regulate wastewater discharge into drains. The parameters of CSR are closely related to those of the potable water after certain advanced treatment which can be reused in many ways. Moreover, it does not require any external aeration and thus the cost associated with energy and devices required for aeration are cut to zero.


1986 ◽  
Vol 96 (2) ◽  
pp. 239-248 ◽  
Author(s):  
D. A. Alabi ◽  
A. A. Adesiyun

SUMMARYWater samples from home filters in nine residential areas of a Nigerian university community were studied. The membrane filter technique was used to determine the total coliform and faecal coliform counts/100 ml of water. Most of the 100 samples studied were grossly contaminated with total coliform counts/100 ml ranging from 0–442, faecal coliform counts/100 ml, 0–216 and the total aerobic plate count per millilitre ranged from 3·0 × 103to 1·9 × 109c.f.u. The source (dams) of water, fitness of filter candles, frequency of cleaning candles and pH of water did not significantly (P> 0·05;X2) affect the microbial quality of either filtered boiled or unboiled tap water.Escherichia colitype I was isolated from 17·9% of the faccal coliforms tested but from only 2·3% of total coliforms.Enterobacter aerogeneswas most predominant (38·5%) amongst faecal coliforms isolated whileEnterobacter cloacaewas the most frequent (37·2%) of the total coliform isolates.The gross contamination of filltered water from all households sampled calls fpr am englightenment of residents on the proper use of home water filters.


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