scholarly journals The Antidiabetic and Antilipidemic Activity of Aqueous Extract of Urtica dioica L. on Type2 Diabetic Model Rats

1970 ◽  
Vol 17 ◽  
pp. 1-6 ◽  
Author(s):  
M Das ◽  
BP Sarma ◽  
AKA Khan ◽  
M Mosihuzzaman ◽  
N Nahar ◽  
...  
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Water Extract ◽  
Treated Group ◽  
Serum Glucose ◽  
Urtica Dioica ◽  
Male Rats ◽  
Diabetic Model ◽  
Type 2 Diabetic

Context: To evaluate the anti-diabetic activity of Urtica dioica L. in type 2 diabetic model rats.Objectives: The aim of this study is to explore the effect of water extract of U. dioica on glycemic status, body weight and lipidemic status in type2 diabetic model ratsMaterials and Methods: The mature and fresh leaves of U. dioica was collected from the mountain range of Assam in India and the leaves extract was used at a dose of 1.25 g-kg body weight. Type2 diabetes was induced by a single intraperitoneal injection of STZ to 48 hours old pups of Long Evans. Experiments for semi-chronic effects were done with continuous feeding of aqueous extract for 14 days at a dose of 1.25 g-kg body weight type 2 diabetic male rats. Serum glucose was estimated by GOD-POD method, serum triglyceride and cholesterol by enzymatic-colorimetric methods.  Results: Water extract showed significant effect on lowering fasting serum glucose levels in type 2 diabetic model rats on 14th day (14.05 ± 3.4 mmol/l in control vs 8.3 ± 1.4 mmol/l in treated group, p = 0.04). There was significant increase in the body weight of treated group in comparison to the control group (175 ± 8 g in control vs in 168 ± 9 g in treated group on 0 day, p = ns; 193 ± 26 g in control vs in 185 ± 25 g in treated group on 14th day, p=0.002]. Beneficial effect was observed in lipid although the change was not statistically significant.Conclusion:  The results obtained in this study suggests that U. dioica has hypoglycemic and hypo-lipidemic activity in type 2 diabetic model rats. This may be due to the histological and functional improvement of b-cells with the consequence of improved insulinemic status.Key words: hypoglycemic; hypolipidemic; Urtica dioica; diabetes mellitusDOI: 10.3329/jbs.v17i0.7092J. bio-sci. 17: 1-6, 2009

scholarly journals Comparative Study on Antidiabetic Effect of Ethanolic Extract of Jute Leaf on Neonatal Streptozotocin- Induced Type-2 Diabetic Model Rat

2020 ◽  
pp. 60-71
Author(s):  
Md. Mahabub Ali ◽  
Md. Asrafuzzaman ◽  
Md. Mahedi Hassan Tusher ◽  
Md. Hafizur Rahman ◽  
Md. Tanvir Rahman ◽  
...  
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Ethanolic Extract ◽  
Liver Glycogen ◽  
Serum Glucose ◽  
Water Control ◽  
Citrate Buffer ◽  
Diabetic Model ◽  
Type 2 Diabetic

Aim: Functional food and their bioactive compounds have been considered as a new approach for the prevention and management of type 2 diabetes and its complications. According to this approach current study was carried out as an elucidation of antidiabetic properties of Corchorus capsularis and Corchorus olitorius varieties of jute leaf (ethanolic extract) on nSTZ-induced type-2 diabetic rats. Methodology: The type-2 diabetic model rat was developed by a single intraperitoneal injection of freshly prepared STZ (90 mg/kg/10 ml) in sterile citrate buffer (0.1 M, pH 4.5) to rat pups (48 hour old). After three months, OGTT was performed to select diabetic (FSG > 6.5mmol/L and after 90 min of glucose load > 14 mmol/L) experimental rats. The rats were randomly divided into four groups [DWC, GT, Ext-1 and Ext-2 represent, diabetic water control, glybenclamide treated (20 mg/5 ml/kg body weight), C. capsularis treated and C. olitorius treated group (1.25 g/10 ml/kg body weight) respectively]. One group was kept with normal rats [normal water control, NWC]. The treatment was given once daily or 28 consecutive days. Fasting serum glucose, liver glycogen and lipid profile were estimated by using standard methods. Results: The results showed that Ext-1 and Ext-2 treated groups gradually decreased serum glucose level (7.15 ±0.67 to 5.94 ± 1.19 and 7.20 ± 0.93 to 5.28 ±1.03 respectively) and reducing effect by Ext-2 was significant (p=0.001). Both extract showed lower liver glycogen level compared with GT group [5.0±2.5 Vs 17.7±6.5 (Ext-1 vs GT) and 7.5±6.4 Vs 17.7±6.5 (Ext-2 vs GT)] and even Ext-1 manifested significant effect (p=0.05). Additionally, lipid profile estimation revealed no significant improvement by the consumption of both the extracts. Conclusion: On the basis of current investigations, it may be concluded that both variety of jute’s leaf demonstrated hypoglycemic properties in Type 2 diabetic model rats; further in-depth studies are recommended to explore the exact mechanism(s) of hypoglycemic effect.

scholarly journals Anti-diabetic effect of Oyster Mushroom mediates through increased AMP-activated protein kinase (AMPK) and cyclic AMP-response element binding (CREB) protein in Type 2 Diabetic model Rats

2018 ◽  
Vol 17 (4) ◽  
pp. 661-668
Author(s):  
Manoj Mandal ◽  
Rakibuzzaman ◽  
Begum Rokeya ◽  
Liaquat Ali ◽  
Zahid Hassan ◽  
...  
Keyword(s):  
Body Weight ◽  
Protein Kinase ◽  
Fasting Glucose ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Oyster Mushroom ◽  
Diabetic Model ◽  
Amp Activated Protein Kinase

AMP-activated protein kinase (AMPK) and c-AMP-response element binding protein (CREB) are found to be important proteins in metabolic system. AMPK has become the focus as a novel therapeutic target for the treatment of metabolic syndromes. Oyster mushroom is traditionally used as remedy of diabetes and hypertension. The present study aims to observe the stimulation of AMPK and CREB in streptozotocin-induced diabetic model rats through Oyster mushroom administration. Long Evan’s rats were used to create type 2 model diabetic rats through intraperitoneal injection of streptozotocin at 90mg/kg body weight of 48hr old pups. Rats were divided into three groups: diabetic control rats, glibenclamide treated diabetic rats (positive control) and mushroom treated diabetic rats (experimental group). Mushroom was administered orally at a dose of 1.25g/kg body weight in semisolid forms. After five weeks rats were sacrificed, serum and tissues were collected for future analysis. Glucose was measured using glucose-oxidase method, lipid profile by enzymaticcolorimetric method. Proteins from different tissues were extracted using RIPA cell lysis buffer, AMPK and CREB were identified using western blot and immuno-precipitation techniques. A significant decreased of fasting glucose was found after 35 days of experiment when it compared with control diabetic rats (M ± SD, mmol/l, Diabetic control group: 8.0±1.1; Mushroom treated diabetic group: 6.4±1.0; p=0.021). Glibenclamide treated diabetic rats have also shown decreased fasting glucose compared to control diabetic rats. In paired ‘t’ test analysis, it has been found that serum fasting glucose level was significantly decreased on 35th day compared the 0 day in both mushroom treated group (p=0.027) and in glibenclamide treated group (p=0.005). Serum TG level was decreased on 35th day compared to 0day in mushroom treated diabetic model rats only (M±SD, mg/dl, 0 day: 84±13; 35th day: 61±6, p=0.002). No significant changes of cholesterol, HDL and LDL were noticed in the experimental groups following treatment with mushroom. Western blot analyses have shown increased band intensity of AMPK and p-CREB in mushroom treated diabetic model rats. Therefore, it can be concluded that Anti-hyperglycemic property of Oyster mushroom could be explained through increased expression of AMPK and activation of CREB.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.661-668

scholarly journals Hypoglycemic effect of Irvingia gabonensis (Aubry-Lacomate Ex. Ororke), Baill in Type 2 Diabetic Long-Evans Rats

2012 ◽  
Vol 11 (1) ◽  
pp. 19-24 ◽  
Author(s):  
M S Hossain ◽  
S Sokeng ◽  
M Shoeb ◽  
K Hasan ◽  
M Mosihuzzaman ◽  
...  
Keyword(s):  
Glucose Level ◽  
Serum Insulin ◽  
Liver Glycogen ◽  
Treated Group ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Bush Mango ◽  
Irvingia Gabonensis ◽  
Type 2 Diabetic

Irvingia gabonensis (Aubry-Lacomate Ex. Ororke), Baill (African wild mango/bush mango) seeds are   widely used in cooking as a sauce in Cameroon and in most parts of tropical Africa for the treatment of a number of ailments. In this study normal rat food was incorporated with I. gabonensis seed powder (10%) and oil free seed powder (5%) and their chronic effects on streptozotocin induced Type 2 diabetic rats were studied. Oral consumption of food incorporated with seed powder significantly reduced serum glucose level on the 28th day (p<0.01) which was   comparable with glibenclamide treated group. Food with oil free seed powder showed 24% fall in glucose level on the 28th day. Fasting serum insulin increased significantly (p<0.001) in glibenclamide and oil free seed powder treated (p<0.008) groups. No effect was observed in the seed powder treated group. Liver glycogen content increased in the glibenclamide treated group but no significant change was observed in both powder and oil free seed powder   treated groups. On the 28th day seed powder treated group significantly lowered serum TG level (p<0.033) and 48% was lowered by oil free seed powder. It is concluded that seed powder as well as oil free seed powder lowered blood glucose level in Type 2 diabetic model rats. It seems to act as an insulinomimetic and/or insulin sensitizing agent. DOI: http://dx.doi.org/10.3329/dujps.v11i1.12482 Dhaka Univ. J. Pharm. Sci. 11(1): 19-24, 2012 (June)

scholarly journals Pharmacological studies of Neopicrorrhiza scrophularii-flora and its antidiabetic effect

2015 ◽  
Vol 27 (1) ◽  
pp. 34-39
Author(s):  
Nayan Manandhar
Keyword(s):  
Water Extract ◽  
Serum Glucose ◽  
Control Group ◽  
Active Principle ◽  
Oral Glucose ◽  
Oral Glucose Load ◽  
Glucose Load ◽  
Animal House ◽  
Type 2 Diabetic

Neopicrorrhiza srophulariiflora (NS), locally known as “kutki / katuki” in nepali is available in 3500-4800 m of Nepal. The present study was carried out to evaluate the antidiabetic property of NS in streptozotocin (STZ) induced type 2 diabetic model rats. NS dried rhizomes, was extracted with 80% ethanol and water by cold percolation method. The extracts were administered at a dose of 1.25gkg-1 body weight for 21 consecutive days to type 2 diabetic male Long-Evans rats, bred at BIRDEM animal house. Serum glucose was estimated by GOD PAP method. Ethanol extract of N. srophulariiflora significantly (p<0.05) improved oral glucose tolerance in type 2 rats in comparison to control group. The water extract and ethanol extracts significantly lowered serum glucose level of type 2 diabetic rats in both prandial states (simultaneously with oral glucose load p<0.05; at 75min and 30 minutes prior to oral glucose load p<0.05; at 105min) compared to control group. N. srophulariiflora is beneficial for treating Type 2 diabetes and therefore needs further exploration and researches, both chemically and biologically to identify the active principle(s) and mechanism of action. DOI: http://dx.doi.org/10.3126/jnpa.v27i1.12148 Journal of Nepal Pharmaceutical Association 2014 Vol.XXVII: 34-39

scholarly journals Protective Effects of Olea europaea Fruit Extracts on Metabolic Disorders Associated With Sucrose-Induced Metabolic Syndrome in Rats

2018 ◽  
Vol 6 (1) ◽  
pp. 8-14
Author(s):  
Akram Ahangarpour ◽  
Fatemeh Ramezani Ali-Akbari
Keyword(s):  
Metabolic Syndrome ◽  
Body Weight ◽  
Aqueous Extract ◽  
Metabolic Disorders ◽  
Density Lipoprotein ◽  
Treated Group ◽  
Serum Glucose ◽  
Protective Effects ◽  
Hydroalcoholic Extract ◽  
Fruit Extracts

Background: Metabolic syndrome (MetS) increases the risk of diabetes. Olea europaea fruit exerts protective effects on metabolic disorders. Therefore, the aim of the present study was to investigate the effect of O. europaea fruit extracts on sucrose-induced MetS in rats. Methods: Male adult Wistar rats (200±50 g, n=32) were randomly divided into four groups (n=8) consisting of control group, untreated sucrose group (sucrose 50% in drinking water for 10 weeks), sucrose plus aqueous extract of O. europaea fruit treated group (200 mg/kg) and sucrose plus hydroalcoholic extract of O. europaea fruit treated group (200 mg/kg) by gavage for 2 weeks. Body weight, serum glucose, insulin, leptin, lipid profile, homeostatic model assessment of insulin resistance (HOMA-IR) and hepatic enzymes were measured. Data were analyzed by one-way analysis of variance (ANOVA, SPSS, 16.0). P<0.05 was regarded as significance level. Results: The aqueous extract exhibited higher protective effects on serum glucose, insulin and HOMA-IR than hydroalcoholic extract (P<0.05). Body weight, serum glucose, leptin (P<0.01), insulin, triglyceride, very-low-density lipoprotein cholesterol (VLDL-C), HOMA-IR, alkaline phosphatase (ALP), (P<0.001) and aspartate aminotransferase (AST) (P<0.05) significantly elevated but high-density lipoprotein cholesterol (HDL-C) (P<0.05) decreased in the sucrose group. Aqueous extract of O. europaea fruit significantly improved blood glucose, triglyceride, VLDL-C (P<0.01), insulin, HOMA-IR, ALP (P<0.001), body weight, AST and leptin (P<0.05) levels. Hydroalcoholic extract of O. europaea fruit significantly restored insulin, HOMA-IR (P<0.01), ALP (P<0.001), body weight, leptin, VLDL-C, triglyceride, blood glucose and AST (P<0.05). Conclusion: Our results indicated O. europaea fruit extracts could improve metabolic disorders induced by MetS in the rats.

scholarly journals Study of Histological Changes in the Bones of Front and Hind Limbs of White Rat Treated with Ibuprofen and Lepidium Sativum

2021 ◽  
Vol 11 (2) ◽  
pp. 1988-2003
Author(s):  
Zainab Salah Abdul Jabbar
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Water Extract ◽  
The Body ◽  
Male Rats ◽  
Lepidium Sativum ◽  
Control Group ◽  
Histological Changes ◽  
Hind Limbs ◽  
Animal House

The current study was conducted to evaluate the morphological and histological changes in the front and hind limbs of Male albino rat belonging to the strain Sprague Dawley, treated with Ibuprofen and the possible protective effects of aqueous extract of Lepidium sativum. The study was performed in the animal house of Department of Biology Collage of Education for Girls University of Kufa. The study lasted seven months from September 2020 until February 2021 by following (80) male rats and were (16-20) weeks that weighted between (200-250)g which was divided in to eight groups each group consist of ten males. The first group was the orally given the normal saline only, and it was control group. The second group was treated orally with Ibuprofen at concentration of (400) mg/kg while the third group was administered Ibuprofen at concentration (400)mg/kg+ aqueous extract of Lepidium sativum seeds with concentration of (50) mg/kg. The fourth group was dosage Ibuprofen at (400)mg/kg+ extract of Lepidium sativum seeds with concentration (100)mg/kg orally too. The fifth group was treated with Ibuprofen at concentration (400)mg/kg +aqueous extract of Lepidium sativum at concentration (150) mg/kg orally. The sixth group was orally given the aqueous extract of Lepidium sativum seeds with concentration (50)mg/kg. The seventh group was treated with the aqueous extract of Lepidium sativum seeds with concentration (100)mg/kg orally. The last group was submitted to the aqueous extract of Lepidium sativum seeds with concentration (150)mg/kg by oral all groups were conducted once day from the first day until the sacrifice which was in two stages on (30 and 45)days. The current study included the process of recording the animal weights of body before and after the completion of experiment as well as weights of bones for front and hind limbs after the end of dose period The study included the histolgical sections of the limbs bones. It was absorved through the macroscopic examination of males dosed with Ibuprofen drug at concentration (400)mg/kg lack of movement and ,anorexia and sluggishness during a period 45 days. The results of statistical analysis showed significant decrease (P<0.05) in the body weight and the weight of limbs bones and the in treatment that were treated with Ibuprofen (30) and (45) days compared with control group. The results also showed decreased significant differences in body weight and weight of limbs in the groups were treated with Ibuprofen + water extract of lepidum sativum seeds (50)mg/kg also the result showed decrease (p<0.05) in body weight in the group of Ibuprofen + water extract of lepidum sativum seeds at (100)mg/kg in, the first dose 30 days, while there was no significant in the same group for the second dose(45) days. As for the group that was dose orally the aqueous extract of Lepidium Sativum seeds (50,100,150) mg/kg and for first and second dose showed significant increase ()in wight body and bones limb but no significant in length body and length of bones. Also patholgical changes were observed in the histolgical section of bone that dosed ibuprofen such as cellular changes in osteocytes, the lacuna looked empty without osteocyt and effected of cell components and cell life. The ather histolgical change was show in the bone marrow that decrease in blood cells components with hypoplasia and replacement of heamatopia with adipose content in the marrow cavity. There was no histopatholgical changes in the groups that treated with Ibuprofen +water extract of lipdium sativum seeds (50,100,150)Mg/kg compared with control group. As well as the group that was treated with water extract of Lepidium sativum showed cellular activity. From this study, it was concluded that Ibuprofen drug has toxic effects in the osteocytes that was affected in the bone tissue and bone formation for long term while the concentration aqueous extract of Lepidium sativum seeds has prevented the uncertain effects of drug. This stuy showed the important role of lepidum sativum as food supplements rich with vitamins and minerals on growth of bones.

scholarly journals The Effect of 12 Weeks Aerobic Training on PDX-1 and GLUT2 Gene Expression in the Pancreatic Tissue of Type 2 Diabetic Rats

2020 ◽  
Author(s):  
Shokoufeh Sokhanvardastjerdi ◽  
Abdolali Banaeifar ◽  
Sajad Arshadi ◽  
Ardeshir Zafari
Keyword(s):  
Gene Expression ◽  
Aerobic Training ◽  
Serum Glucose ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Male Rats ◽  
P Value ◽  
Glut2 Gene ◽  
Type 2 Diabetic

Objective: The aim of this study was to investigate the effect of 12 weeks aerobic training on PDX-1 and GLUT2 gene expression in the pancreatic tissue of type 2 diabetic rats. Materials and Methods: 21 wistar male rats were placed in 3 groups (healthy, diabetic, aerobic diabetic). Diabetes was induced by peritoneum injection of nicotine amid. Training program lasted 12 weeks, five sessions per week by gradual increase of speed (18 to 26 meters on minutes) and time (10 to 55 minutes) in the form of running on the treadmill. After 12 week aerobic training, PDX-1 and GLUT-2 genes were measured. Data was analyzed using ANOVA with 5 % significance level. Results: Aerobic training caused a significant increase in the expression of GLUT2 (P-value: 0.043) and PDX-1 genes (P-value: 0.007) in the pancreatic tissue of rats with type 2 diabetes (p value: 0.05). Also aerobic training had a significant effect on serum glucose (P-value: 0.001) and insulin levels (P-value: 0.001). Conclusion: It is concluded that aerobic training has significant effects on diabetic control by increasing the expression of PDX1 and GLUT-2 which lowers serum glucose.

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