scholarly journals Isolation and Identification of Pathogenic Gram-Positive Bacteria from Egg Shell of Hen and to See Their Antimicrobial Susceptibility Pattern

2016 ◽  
Vol 6 (1) ◽  
pp. 15-18 ◽  
Author(s):  
Jannatul Fardows ◽  
Abu Bakar Siddique ◽  
Adneen Moureen ◽  
Tasmin Afroz Binte Islam ◽  
Nasrin Farhana ◽  
...  
Keyword(s):  
Public Health Problem ◽  
Major Public Health Problem ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern ◽  
Gram Positive Bacteria ◽  
Source Of Infection ◽  
Egg Shell ◽  
Staphylococcus Spp

Background: Food-borne disease is a major public health problem affecting developed as well as developing countries. Inaccurately treated eggs can be one of its causes. So we designed this study to observe the possibility of transmission of pathogenic Gram-positive bacteria from market eggs to the community.Objectives: To identify different Gram-positive bacteria in eggs and to observe their antimicrobial susceptibility.Materials and Methods: This observational study was conducted in the department of Microbiology, Dhaka Medical College, Dhaka. Shells of 150 eggs collected from different markets of Dhaka city were tested. Bacteria were isolated and identified by culture and relevant biochemical tests.Results: Out of 150 egg shells, 120 (80%) yielded growth of different bacteria. Of them, Staphylococcus spp. were 80 (66.67%), Streptococcus spp. 8 (6.67%), Bacillus subtilis 20 (16.67%) and Bacillus cereus 12 (10%). Out of 80 Staphylococcus spp., 30 (25%) were Staphylococcus aureus and 50 (41.67%) were Staphylococcus saprophyticus. Most of the Gram-positive bacteria were sensitive to ciprofloxacin, ceftriaxone and imipenem. No MRSA and VRSA were found.Conclusion: It can be concluded from this study that Gram-positive bacteria from market eggs may be an important source of infection to the community.J Enam Med Col 2016; 6(1): 15-18

scholarly journals Biofilm-Related Infections in Gram-Positive Bacteria and the Potential Role of the Long-Acting Agent Dalbavancin

2021 ◽  
Vol 12 ◽  
Author(s):  
Alessandra Oliva ◽  
Stefania Stefani ◽  
Mario Venditti ◽  
Enea Gino Di Domenico
Keyword(s):  
Public Health Problem ◽  
Major Public Health Problem ◽  
Bacterial Cells ◽  
Gram Positive ◽  
Methicillin Resistant ◽  
Gram Positive Bacteria ◽  
Wide Range ◽  
Long Acting ◽  
Acting Agent ◽  
Biofilm Development

Infections caused by Gram-positive bacteria are a major public health problem due to their increasing resistance to antibiotics. Staphylococcus and Enterococcus species’ resistance and pathogenicity are enhanced by their ability to form biofilm. The biofilm lifestyle represents a significant obstacle to treatment because bacterial cells become highly tolerant to a wide range of antimicrobial compounds normally effective against their planktonic forms. Thus, novel therapeutic strategies targeting biofilms are urgently needed. The lipoglycopeptide dalbavancin is a long-acting agent for treating acute bacterial skin and skin structure infections caused by a broad range of Gram-positive pathogens. Recent studies have shown promising activity of dalbavancin against Gram-positive biofilms, including methicillin-resistant S. aureus (MRSA), methicillin-resistant S. epidermidis (MRSE), and vancomycin-susceptible enterococci. This review outlines the mechanisms regulating biofilm development in Staphylococcus and Enterococcus species and the clinical impact of biofilm-related infections. In addition, it discusses the clinical implications and potential therapeutic perspectives of the long-acting drug dalbavancin against biofilm-forming Gram-positive pathogens.

scholarly journals Analysis of clinical profile of women presenting with pyrexia in pregnancy

2018 ◽  
Vol 7 (4) ◽  
pp. 1406
Author(s):  
Surabhi Porwal ◽  
Amita Sharma
Keyword(s):  
Clinical Symptoms ◽  
Public Health Problem ◽  
Clinical Profile ◽  
Major Public Health Problem ◽  
Abdominal Wall Defects ◽  
Chi Square ◽  
Medical College ◽  
Central Nervous System Defects ◽  
High Risk Situation ◽  
In Pregnancy

Background: Pyrexia in pregnancy is major public health problem in India. Pyrexia in pregnancy is associated with resorption of the embryo, foetal deaths and potentially lethal malformations such as central nervous system defects, abdominal-wall defects, and cardiovascular malformations. This study was carried out to analyse clinical profile of women presenting with pyrexia during pregnancy.Methods: After approval by institutional ethical committee the prospective case control study was conducted in the department of obstetrics and gynecology, NSCB Medical College, Jabalpur (M.P.) from 1st June 2012 to 31st October 2013. Total 100 antenatal women with pyrexia taken as cases and 50 antenatal women without pyrexia taken as control were included and analyzed in this study. In women fulfilling inclusion criteria detailed history was taken and documented in proforma. Required investigations were sent to the department of pathology and virology laboratory of Indian Council of Medical Research, Jabalpur. Results were analyzed statistically by using t test and chi square test.Results: On analysis of clinical symptoms pyrexia, cough, malaise, rash, headache, nausea and vomiting, joint pain, anorexia, breathlessness and burning and frequency of micturition were significantly higher in cases as compare to control. Viral pathogens were responsible for most of the cases of pyrexia in pregnancy.Conclusions: Pyrexia in pregnancy is a high-risk situation, early identification and prompt treatment will reduce maternal and perinatal morbidity and mortality associated with pyrexia in pregnancy.

scholarly journals ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH DECOMPOSING PIG (Sus scrofa) CARCASS

2021 ◽  
Vol 4 (2) ◽  
pp. e126
Author(s):  
Iyabo Victoria Olatubi ◽  
Olukemi Aromolaran ◽  
Samuel Tolani Joseph ◽  
Oluwafeyikemi Ajoke Adeleke
Keyword(s):  
Sus Scrofa ◽  
Skin Surface ◽  
Isolation And Identification ◽  
Forensic Biology ◽  
Gram Positive Bacteria ◽  
Average Temperature ◽  
Baseline Information ◽  
Identification Of Bacteria ◽  
Local Farm ◽  
Staphylococcus Spp

The emerging field of forensic biology has attempted to solve certain problems encountered when estimating post-mortem interval (PMI) by using predictable changes in the microbial and arthropod community structure. Pig (Sus scrofa) carcasses are widely used as animal models in clinical human studies. The objective of this study was to identify bacteria from the skin surface of pig carcass for possible use in forensic investigation. Three pigs (a suitable human substitute) were collected from a local farm and killed by suffocation and further place in a bush land for decomposition. 24hours later skin samples were collected and transported to the laboratory for the isolation of bacteria using standard pour plate techniques and identified using Bergey’s manual of systemic bacteriology. The experiment was conducted in February 2019 during the dry season of the year with an average temperature of 23.50c and relative humidity of 60.8% A total of fourteen (14) isolates were gotten from the pig carcass samples out of which four (4) were Gram-positive bacteria and the remaining ten (10) were Gram-negative. Staphylococcus spp. (28.6%) was the most abundant while Salmonella sp., Serratia sp., Klebsiella sp., Citrobacter sp. and Proteus sp. occurred at 14.3% each. This study focus on the type of bacteria communities during a decomposition process which will help provide baseline information in the application of forensic biology to determination of nature of death, abuse or neglect.

scholarly journals Knowledge, attitude and practice regarding various tobacco products and their effects on health and COTPA act among ≥15 years age group persons in urban field practice area of a medical college in Telangana

2020 ◽  
Vol 7 (6) ◽  
pp. 2180
Author(s):  
Jyothi Veleshala ◽  
Varun Malhotra
Keyword(s):  
Tobacco Use ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Community Based ◽  
Cross Sectional ◽  
Tobacco Products ◽  
Medical College ◽  
Attitudes And Practices ◽  
Supply And Distribution ◽  
Almost All

Background: Tobacco use is a major public health problem. The prevalence of tobacco use among men has been reported to be high (generally exceeding 50%) from almost all parts of India. “The Cigarettes and Other Tobacco Products (Prohibition of Advertisement and Regulation of Trade and Commerce, Production, Supply, and Distribution) Act 2003”, which came into effect since 1st May 2004. Information on knowledge, attitudes, and practices of population regarding COTPA is necessary to effectively implement the legislation. Hence the present study was undertaken.Methods: The present study was a cross-sectional community-based survey carried out in urban slums of Nalgonda town. Data was collected using a structured schedule by interviewing 300 participants, aged above 15 years.Results: The current use of any tobacco was reported by 45.7% of the participants. Overall awareness of COTPA was 58.2%. Marital status, type of family, unemployment, Illiterates, lower SES, and age had a significant influence on tobacco use. Television (77.05%) was the major source of awareness regarding COTPA. The majority of smokers were aware of various sections of COTPA (>50%). About 34.8% of the participants had a favorable attitude towards COTPA. The positive attitude was more (53.7%) towards the display of health warnings on tobacco products among smokers. 11.2% had paid penalty for violation of the act and 32.4% have noticed a reduction in their habit to some extent.Conclusions: A concerted effort has to be made to increase the awareness of the act amongst the vulnerable population.

scholarly journals Clinico-epidemiology, malacology and community awareness of Schistosoma mansoni in Haradenaba and Dertoramis kebeles in Bedeno district, eastern Ethiopia

2018 ◽  
Vol 6 ◽  
pp. 205031211878674 ◽  
Author(s):  
Jemal Mohammed ◽  
Fitsum Weldegebreal ◽  
Zelalem Teklemariam ◽  
Habtamu Mitiku
Keyword(s):  
Confidence Interval ◽  
Schistosoma Mansoni ◽  
Odds Ratio ◽  
Adjusted Odds Ratio ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Eastern Ethiopia ◽  
Community Awareness ◽  
Source Of Infection ◽  
Study Participants

Background: Schistosoma mansoni is a major public health problem. Different studies reported unidentified sites in Ethiopia with variable prevalence and intensity, but there is no report from this study area. Objective: To assess the clinico-epidemiology, malacology, risk factors, and community awareness of S. mansoni in Haradenaba and Dertoramis kebeles in the Bedeno district, eastern Ethiopia. Methods: A cross-sectional study was conducted among 1011 study participants in 413 systematically selected households from Haradenaba and Dertoramis kebeles from 30 June 2016 to 30 July 2017. Data were collected by using pretested, structured questionnaires, clinical examinations, and stool examinations. Stool samples were processed by the Kato-Katz method and examined microscopically. Snails were collected by scooping from water contact points. Data were analyzed using SPSS, version 16 statistical software. Result: The overall prevalence of S. mansoni was 35.7%. Heavy-intensity infection was detected in 8.9% of the study participants. Results included: participants who were unaware about the possible source of infection (adjusted odds ratio: 2.95; 95% confidence interval: 1.25, 7.95), modes of transmission (adjusted odds ratio: 5.51; 95% confidence interval: 3.52, 12.51), prevention (adjusted odds ratio: 4.01; 95% confidence interval: 2.00, 8.75) about schistosomiasis/bilharziasis were more likely infected with S. mansoni than those who were aware. Participants who swim or bathe in the river were more likely infected with S. mansoni than those who do not (adjusted odds ratio: 6.41; 95% confidence interval: 3.15, 11.25). Biomphalaria pfeifferi snails were collected from all water bodies in Haradenaba and Dertoramis, but they did not shed schistosome cercaria in the laboratory. Conclusion: S. mansoni infection is found in high magnitude in these study areas, despite not being previously reported. A majority of the study participants had low awareness about the source of infection, mode of transmission, and prevention methods of schistosomiasis. Efforts should be made to improve community awareness about transmission and prevention of schistosomiasis.

scholarly journals Isolation and identification of membrane vesicle-associated proteins in Gram-positive bacteria and mycobacteria

MethodsX ◽  
2014 ◽  
Vol 1 ◽  
pp. 124-129 ◽  
Author(s):  
Rafael Prados-Rosales ◽  
Lisa Brown ◽  
Arturo Casadevall ◽  
Sandra Montalvo-Quirós ◽  
Jose L. Luque-Garcia
Keyword(s):  
Membrane Vesicle ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Associated Proteins

scholarly journals Isolation and identification of halophilic and halotolerant bacteria from the sediments of the Qeshm Island mangrove forest

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Pegah Javid ◽  
Hassan Zadabbas Shahabadi ◽  
Homeyra Amirkhani ◽  
Narges Amrollahi ◽  
Mohammad Sharif Ranjbar
Keyword(s):  
Halophilic Bacteria ◽  
Mangrove Forests ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Qeshm Island ◽  
Gram Positive ◽  
Halotolerant Bacteria ◽  
Gram Positive Bacteria ◽  
Phylogenetic Taxonomy

Due to specific environmental and ecological conditions, mangrove forests are known as marine transitional zones between sea and land, and, as such, they host organisms with high ecological plasticity. The mangrove forests of Qeshm Island (Iran) are relatively pristine habitats and represent an ideal target for investigating patterns of either aquatic or benthic biodiversity. To provide insights on microbial diversity in this area, nineteen halophilic and halotolerant bacteria were isolated from the sediments in 2017 during low tide. The extracted bacterial strains were studied morphologically by streaking, initial observation of colonies and bacterial staining, and characterized using a battery of biochemical tests including KOH, MR, VP, urease, TSI, S/I/M, Mac, LIA, ODC, ADH, oxidase, catalase, and tryptophan deaminase. The optimum growth of halophilic bacteria was observed in salt concentrations from 5 to 20% NaCl, whereas the extreme halophilic Gram-positive strain grew in salt concentration of up to 30% NaCl. Molecular analyses were also carried out on four halophilic strains and one extreme halophilic gram-positive bacteria. Phylogenetic taxonomy analysis, after 16S rDNA gene Sanger sequencing, revealed that the halophilic bacteria were closely related to the strain types of the genus Bacillus including Bacillus licheniformis, Bacillus velezensis, Bacillus Paralicheniformis and Bacillus sp. with 99% bootstrap value. The extreme halophilic strain was associated to strains of Planococcus plakortidis with 100% bootstrap value.

Isolation and Identification of Bacteria from Marine Biofilms

2005 ◽  
Vol 277-279 ◽  
pp. 612-617 ◽  
Author(s):  
Yoo Kyung Lee ◽  
Kae Kyoung Kwon ◽  
Kyeung Hee Cho ◽  
Jae Hyun Park ◽  
Hong Kum Lee
Keyword(s):  
Culturable Bacteria ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Rdna Sequences ◽  
Marine Biofilms ◽  
Antifouling Agent ◽  
Identification Of Bacteria ◽  
Invertebrate Larvae

In the marine environment, biofilms cover most of the subtidal and intertidal solid surfaces. Culturable bacteria forming marine biofilms were isolated on artificial substrate called acrylic coupons. The bacterial isolates were identified through a comparison of 16S rDNA sequences. A total of 115 strains were cultured and identified, 45 of which showed the same sequences with other strains. Therefore, 70 strains were finally identified. The bacterial isolates belonged to a–Proteobacteria (32 isolates), g–Proteobacteria (12 isolates), CFB group bacteria (4 isolates), high GC Gram-positive bacteria (9 isolates), and low GC Gram-positive bacteria (13 isolates). The bacterial isolates may be used as standard bacteria to test new antifouling agent. They may also be utilized as useful bacteria to enhance the settlement of commercial algae and invertebrate larvae for aquaculture.

scholarly journals Study of association and prognostic correlation of cardiac troponin I estimation in acute decompensated heart failure patients

2020 ◽  
Vol 8 (1) ◽  
pp. 26
Author(s):  
Sunil Kumar Tripathi ◽  
Vikas A. Mishra ◽  
Amit B. Kinare ◽  
Vishwa Deepak Tripathi ◽  
Ravi Shankar Sharma
Keyword(s):  
Heart Failure ◽  
Troponin I ◽  
Acute Decompensated Heart Failure ◽  
Public Health Problem ◽  
Decompensated Heart Failure ◽  
Major Public Health Problem ◽  
Positive Group ◽  
Medical College ◽  
Follow Up Study

Background: Heart failure is a major public health problem since last few decades affecting significant number of people worldwide. Acute decompensated heart failure is a major cause of hospitalization in elderly people with a high mortality rate. Heterogeneity and non-specificity of symptoms makes diagnosis of heart failure by clinical presentation alone more challenging. Aim of current study was to investigate troponin biomarkers in diagnosis, prognosis and management of acute decompensated heart failure.  Methods: Present study was a prospective observational study conducted on 100 patients at Department of Cardiology, Superspeciality hospital, NSCB medical college Jabalpur and Department of cardiology Superspeciality hospital, SS medical college Rewa from October 2019 to August 2020. Patients were investigated for clinical, echocardiographic parameters and NYHA classification. Cardiac functions were analyzed by color doppler echocardiography. Results: According to study findings, 65.2% of TnI positive patients were males whereas 34.8% were females. Mean age of TnI positive group was observed to be higher. Majority of troponin positive patients were in NYHA class IV. Recurrent hospitalization was observed more in TnI positive group. Logistic regression analysis depicted systolic blood pressure reduced significantly (p<0.001) on follow up study in TnI positive patients, FBS was significantly more in TnI positive patients (131.4+42.9 mg/dl) (p=0.049). LVID was significantly more in TnI positive patients (p=0.022). Reduction in EF was statistically significant (p=0.03) at the three months follow up study.  Conclusions: A positive prognostic correlation was established between ADHF and troponin positivity, large prospective randomized trials are necessary to recommend quantitative troponin I determination in all patients of acute decompensated heart failure for prognosis and guiding therapy.

scholarly journals Renal Function Evaluation of Hypertensive Patients by Renogram in a Teaching Hospital

2016 ◽  
Vol 11 (1) ◽  
pp. 11-14
Author(s):  
Jayanta Kumar Saha ◽  
ARM Saifuddin Ekram ◽  
Mohammed Shahadat Hossain ◽  
ATM Ataur Rahman ◽  
Prodip Kumar Biswas ◽  
...  
Keyword(s):  
Renal Function ◽  
Renal Involvement ◽  
Public Health Problem ◽  
Function Evaluation ◽  
Major Public Health Problem ◽  
College Hospital ◽  
Cross Sectional ◽  
Female Ratio ◽  
Hypertensive Patients ◽  
Medical College

Arterial Hypertension is the most common cardiovascular disease and is a major public health problem in both developed and developing countries. The aim of the study was to assess renal involvement in hypertensive patient by renogram, which is not detected by conventional biochemical test. This was a cross sectional observational study. Consecutive 30 hypertensive patients admitted in the medicine wards from March, 2006 to April, 2007 in Rajshahi Medical College Hospital, Bangladesh were taken as case. Informed consent was taken from all the study patients or from their attendants. Detail history was taken from each patient or from their attendants' then clinical examination and relevant investigations were done. Emphasis was laid to find out renal involvement in long standing hypertensive patients by renogram. After initial selection; laboratory investigations like Urine for R/M/E, Blood Sugar 2ABF, S. Creatinine, USG of KUB, ECG and Hb% were done for every patients. All patients included in this study renogram were done. Majority of the patient (40%) were between the age of 41-50 years and Male: Female ratio was 3:2. Most of the patient lead a moderately active life (66.67%). Serum Creatinine was raised in 23.33% patient whereas renal parenchymal insufficiency evidenced by renogram was found in 50% case. In this study, we found that a significant number of cases (26.67%) having renal parenchymal insufficiency were not detected by S. Creatinine level, but detected by renogram. Renogram is superior to conventional biochemical renal function test for early detection of hypertensive nephropathy.Faridpur Med. Coll. J. Jan 2016;11(1): 11-14

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