Isolation and Identification of Bacteria from Marine Biofilms

In the marine environment, biofilms cover most of the subtidal and intertidal solid surfaces. Culturable bacteria forming marine biofilms were isolated on artificial substrate called acrylic coupons. The bacterial isolates were identified through a comparison of 16S rDNA sequences. A total of 115 strains were cultured and identified, 45 of which showed the same sequences with other strains. Therefore, 70 strains were finally identified. The bacterial isolates belonged to a–Proteobacteria (32 isolates), g–Proteobacteria (12 isolates), CFB group bacteria (4 isolates), high GC Gram-positive bacteria (9 isolates), and low GC Gram-positive bacteria (13 isolates). The bacterial isolates may be used as standard bacteria to test new antifouling agent. They may also be utilized as useful bacteria to enhance the settlement of commercial algae and invertebrate larvae for aquaculture.

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Influence of Cell Wall Composition on the Fossilisation of Bacteria and the Implications for the Search for Early Life Forms

International Astronomical Union Colloquium ◽

10.1017/s0252921100015025 ◽

1997 ◽

Vol 161 ◽

pp. 491-504 ◽

Cited By ~ 3

Author(s):

Frances Westall

Keyword(s):

Cell Wall ◽

Life Forms ◽

Cation Binding ◽

Positive Bacterium ◽

Gram Positive ◽

Gram Positive Bacteria ◽

Transmission Electron ◽

Hydrothermal Sediments ◽

Identification Of Bacteria ◽

The Difference

AbstractThe oldest cell-like structures on Earth are preserved in silicified lagoonal, shallow sea or hydrothermal sediments, such as some Archean formations in Western Australia and South Africa. Previous studies concentrated on the search for organic fossils in Archean rocks. Observations of silicified bacteria (as silica minerals) are scarce for both the Precambrian and the Phanerozoic, but reports of mineral bacteria finds, in general, are increasing. The problems associated with the identification of authentic fossil bacteria and, if possible, closer identification of bacteria type can, in part, be overcome by experimental fossilisation studies. These have shown that not all bacteria fossilise in the same way and, indeed, some seem to be very resistent to fossilisation. This paper deals with a transmission electron microscope investigation of the silicification of four species of bacteria commonly found in the environment. The Gram positiveBacillus laterosporusand its spore produced a robust, durable crust upon silicification, whereas the Gram negativePseudomonas fluorescens, Ps. vesicularis, andPs. acidovoranspresented delicately preserved walls. The greater amount of peptidoglycan, containing abundant metal cation binding sites, in the cell wall of the Gram positive bacterium, probably accounts for the difference in the mode of fossilisation. The Gram positive bacteria are, therefore, probably most likely to be preserved in the terrestrial and extraterrestrial rock record.

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ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH DECOMPOSING PIG (Sus scrofa) CARCASS

Bacterial Empire ◽

10.36547/be.126 ◽

2021 ◽

Vol 4 (2) ◽

pp. e126

Author(s):

Iyabo Victoria Olatubi ◽

Olukemi Aromolaran ◽

Samuel Tolani Joseph ◽

Oluwafeyikemi Ajoke Adeleke

Keyword(s):

Sus Scrofa ◽

Skin Surface ◽

Isolation And Identification ◽

Forensic Biology ◽

Gram Positive Bacteria ◽

Average Temperature ◽

Baseline Information ◽

Identification Of Bacteria ◽

Local Farm ◽

Staphylococcus Spp

The emerging field of forensic biology has attempted to solve certain problems encountered when estimating post-mortem interval (PMI) by using predictable changes in the microbial and arthropod community structure. Pig (Sus scrofa) carcasses are widely used as animal models in clinical human studies. The objective of this study was to identify bacteria from the skin surface of pig carcass for possible use in forensic investigation. Three pigs (a suitable human substitute) were collected from a local farm and killed by suffocation and further place in a bush land for decomposition. 24hours later skin samples were collected and transported to the laboratory for the isolation of bacteria using standard pour plate techniques and identified using Bergey’s manual of systemic bacteriology. The experiment was conducted in February 2019 during the dry season of the year with an average temperature of 23.50c and relative humidity of 60.8% A total of fourteen (14) isolates were gotten from the pig carcass samples out of which four (4) were Gram-positive bacteria and the remaining ten (10) were Gram-negative. Staphylococcus spp. (28.6%) was the most abundant while Salmonella sp., Serratia sp., Klebsiella sp., Citrobacter sp. and Proteus sp. occurred at 14.3% each. This study focus on the type of bacteria communities during a decomposition process which will help provide baseline information in the application of forensic biology to determination of nature of death, abuse or neglect.

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Isolation and Identification of Hydrocarbon-Degrading Bacteria that Tolerant to Saponin of Sapindus Rarak Plant

Jurnal Biodjati ◽

10.15575/biodjati.v4i1.4392 ◽

2019 ◽

Vol 4 (1) ◽

pp. 79-88

Author(s):

Evi Octaviany ◽

Suharjono Suharjono ◽

Irfan Mustafa

Keyword(s):

Crude Oil ◽

16S Rdna ◽

Bacterial Isolates ◽

High Concentration ◽

Isolation And Identification ◽

Rdna Sequences ◽

Degrading Bacteria ◽

16S Rdna Sequences ◽

Petroleum Contaminated Soil ◽

Cell Densities

A commercial saponin as biosurfactant can reduce the surface tension of water and increase of hydrocarbon degradation. However, this saponin can be toxic to some hydrocarbonoclastic bac-teria. This study aimed to obtain bacterial isolates that were tolerant and incapable to degrade saponin, and to identify them based on 16S rDNA sequence. Bacteria were isolated from petroleum contaminated soil in Wonocolo Village, Bojonegoro Regency, East Java, Indonesia. The soil samples were acclimated using Bushnell-Haas (BH) broth with 0.5% crude oil at room temperature for 3 weeks. The culture was spread onto BH agar incubated at 30°C for 7 days. The first screened, isolates were grown in nutrient broth with addition of sap-onin 0%, 8%, and 12% (v/v) then incubated at 30°C for three days. The bacterial cell density was measured using a spectrophotometer. Second screened, the isolates were grown on BH broth with addition of 0.5% saponin as a sole carbon source, and their cell densities were measured. The selected isolates were identified based on 16S rDNA sequences. Among 34 bacterial isolates, nine isolates were tol-erant to 12% saponin. Three bacterial isolates IHT1.3, IHT1.5, and IHT3.24 tolerant to high concentration of saponin and did not use this substance as growth nutrition. The IHT1.3, IHT1.5, and IHT3.24 isolates were identified as Ochrobactrum pseudogrignonense (99% similarity), Pseudomonas mendocina (99%), and Ochrobactrum pi-tuitosum; (97%), respectively. Those three selected isolates are good candidates as hydrocarbon-degrading bacteria to bioremediation of soil contaminated crude oil. However, the combined activity of bacteria and saponin to degrade hydrocarbon needs further study.

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Isolation and identification of membrane vesicle-associated proteins in Gram-positive bacteria and mycobacteria

MethodsX ◽

10.1016/j.mex.2014.08.001 ◽

2014 ◽

Vol 1 ◽

pp. 124-129 ◽

Cited By ~ 18

Author(s):

Rafael Prados-Rosales ◽

Lisa Brown ◽

Arturo Casadevall ◽

Sandra Montalvo-Quirós ◽

Jose L. Luque-Garcia

Keyword(s):

Membrane Vesicle ◽

Isolation And Identification ◽

Gram Positive ◽

Gram Positive Bacteria ◽

Associated Proteins

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Disruption of Firmicutes and Actinobacteria abundance in tomato rhizosphere causes the incidence of bacterial wilt disease

The ISME Journal ◽

10.1038/s41396-020-00785-x ◽

2020 ◽

Vol 15 (1) ◽

pp. 330-347 ◽

Cited By ~ 1

Author(s):

Sang-Moo Lee ◽

Hyun Gi Kong ◽

Geun Cheol Song ◽

Choong-Min Ryu

Keyword(s):

Bacterial Wilt ◽

Rhizosphere Soil ◽

Plant Protection ◽

Plant Immunity ◽

Disease Suppression ◽

Culturable Bacteria ◽

Gram Positive ◽

Gram Positive Bacteria ◽

Individual Strain ◽

Bacillus Niacini

AbstractEnrichment of protective microbiota in the rhizosphere facilitates disease suppression. However, how the disruption of protective rhizobacteria affects disease suppression is largely unknown. Here, we analyzed the rhizosphere microbial community of a healthy and diseased tomato plant grown <30-cm apart in a greenhouse at three different locations in South Korea. The abundance of Gram-positive Actinobacteria and Firmicutes phyla was lower in diseased rhizosphere soil (DRS) than in healthy rhizosphere soil (HRS) without changes in the causative Ralstonia solanacearum population. Artificial disruption of Gram-positive bacteria in HRS using 500-μg/mL vancomycin increased bacterial wilt occurrence in tomato. To identify HRS-specific and plant-protective Gram-positive bacteria species, Brevibacterium frigoritolerans HRS1, Bacillus niacini HRS2, Solibacillus silvestris HRS3, and Bacillus luciferensis HRS4 were selected from among 326 heat-stable culturable bacteria isolates. These four strains did not directly antagonize R. solanacearum but activated plant immunity. A synthetic community comprising these four strains displayed greater immune activation against R. solanacearum and extended plant protection by 4 more days in comparison with each individual strain. Overall, our results demonstrate for the first time that dysbiosis of the protective Gram-positive bacterial community in DRS promotes the incidence of disease.

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Isolation and identification of halophilic and halotolerant bacteria from the sediments of the Qeshm Island mangrove forest

Advances in Oceanography and Limnology ◽

10.4081/aiol.2020.8743 ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Pegah Javid ◽

Hassan Zadabbas Shahabadi ◽

Homeyra Amirkhani ◽

Narges Amrollahi ◽

Mohammad Sharif Ranjbar

Keyword(s):

Halophilic Bacteria ◽

Mangrove Forests ◽

Bacterial Strains ◽

Biochemical Tests ◽

Isolation And Identification ◽

Qeshm Island ◽

Gram Positive ◽

Halotolerant Bacteria ◽

Gram Positive Bacteria ◽

Phylogenetic Taxonomy

Due to specific environmental and ecological conditions, mangrove forests are known as marine transitional zones between sea and land, and, as such, they host organisms with high ecological plasticity. The mangrove forests of Qeshm Island (Iran) are relatively pristine habitats and represent an ideal target for investigating patterns of either aquatic or benthic biodiversity. To provide insights on microbial diversity in this area, nineteen halophilic and halotolerant bacteria were isolated from the sediments in 2017 during low tide. The extracted bacterial strains were studied morphologically by streaking, initial observation of colonies and bacterial staining, and characterized using a battery of biochemical tests including KOH, MR, VP, urease, TSI, S/I/M, Mac, LIA, ODC, ADH, oxidase, catalase, and tryptophan deaminase. The optimum growth of halophilic bacteria was observed in salt concentrations from 5 to 20% NaCl, whereas the extreme halophilic Gram-positive strain grew in salt concentration of up to 30% NaCl. Molecular analyses were also carried out on four halophilic strains and one extreme halophilic gram-positive bacteria. Phylogenetic taxonomy analysis, after 16S rDNA gene Sanger sequencing, revealed that the halophilic bacteria were closely related to the strain types of the genus Bacillus including Bacillus licheniformis, Bacillus velezensis, Bacillus Paralicheniformis and Bacillus sp. with 99% bootstrap value. The extreme halophilic strain was associated to strains of Planococcus plakortidis with 100% bootstrap value.

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Study of Microbiological Quality of Biscuits from Street Side Bakery Shops at Dehradun City, India

International Journal of Scientific Research in Science and Technology ◽

10.32628/ijsrst207348 ◽

2020 ◽

pp. 319-326

Author(s):

Irtiqa Syed ◽

Rajendra Prasad ◽

Adeeba Naaz

Keyword(s):

Agar Medium ◽

Microbiological Quality ◽

Nutrient Agar ◽

Morphological Observation ◽

Plate Count ◽

Bacterial Isolates ◽

Gram Positive ◽

Gram Positive Bacteria ◽

Nutrient Agar Medium

<p>In the present study, microbiological quality of biscuits from street side bakery shops at Dehardun city, India was conducted. A total 11 samples of bakery biscuits were collected randomly and analysed for their microbiologically quality by standard plate count method. Pure cultures of bacterial isolates were prepared by streaking on nutrient agar medium. Bacterial isolates were further studied for morphological characters, culture characters on nutrient agar medium and biochemical testes in laboratory. All samples studies were found contaminated by a variety of bacteria. The highest bacterial load was 2.2 ×105 cfu/g and lowest was 0.1×103 cfu/g, respectively. Morphological observation, culture characters and results of biochemical tests of bacterial isolates were compared with standard results of known bacteria. The bacterial isolated were identified as Alcaligenes faecalis, Bacillus cereus, Micrococcus luteus, Pseudomonas aeruginosa, Streptococcus lactis and Staphylococcus aureus, respectively. Among these, two isolates i.e., A. faecalis and P. aeruginosa were gram negative and other isolates were gram positive bacteria which suggested the poor hygienic conditions inside the bakery during preparation and storage of biscuits. Gram positive bacteria might be contaminated during storage and by bad handling. These food spoiling bacteria might be responsible for food-borne infection and diseases. Therefore, care should be taken while purchasing such products and should be purchased from certified bakery shop.</p>

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Isolation and Identification of Bacteria in the Mouth Before and After Ablution

Green Medical Journal ◽

10.33096/gmj.v3i3.89 ◽

2021 ◽

Vol 3 (3) ◽

pp. 132-138

Author(s):

Fitriah Fitriah ◽

Mochammad Erwin Rachman ◽

Sri Wahyuni Gayatri ◽

Fendy Dwimartyono ◽

Hasta Handayani Idrus

Keyword(s):

Oral Health ◽

The Body ◽

Personal Hygiene ◽

Pseudomonas Sp ◽

Normal Flora ◽

Isolation And Identification ◽

Gram Positive Bacteria ◽

Before And After ◽

Identification Of Bacteria ◽

Number Of Bacteria

Background: The oral is the gateway for the entry of various kinds of microorganisms into the body, with the prevalence of people having dental and oral problems in Indonesia increasing every year. The normal flora of the oral acts as a body defense, but it can cause disease due to predisposing factors, namely oral hygiene. Therefore, it is necessary to find an alternative in maintaining oral health. Islam is a religion that emphasizes personal hygiene, such as performing ablution. Content: The types of bacteria found in the oral before ablution was 33.33% Pseudomonas sp., 6.67% Lactobacillus sp., 3.33% Streptococcus sp. and 0.14% Staphylococcus sp. while the types of bacteria found in the oral after ablution were 26,8% Pseudomonas sp., 20% Lactobacillus sp., 5% Streptococcus sp. and 2% Staphylococcus sp. Conclusion: There was a change in the number of bacteria, namely an increase in gram-positive bacteria in the oral after ablution.

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Bakteri Aerob Penyebab Infeksi Nosokomial di Ruang Bedah RSU Abepura, Kota Jayapura, Papua

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.537 ◽

2018 ◽

Vol 4 (2) ◽

pp. 63-68

Author(s):

Daniel Lantang ◽

Desi Paiman

Keyword(s):

Listeria Monocytogenes ◽

Nosocomial Infection ◽

Brain Heart Infusion ◽

Aerobic Bacteria ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Agar Media ◽

Identification Of Bacteria

The research on identification of aerobic bacteria causing nosocomial infection in the surgery room of General Hospital (RSU) Abepura, Jayapura had been done from August–December 2008. Samples were taken from room air (30 minutes) using blood agar media and from certain tools at some points using brain heart infusion media (BHI). The colony appearance was observed after 24 hours of incubation period of bacterial growth. Further analysis to identify the aerobic bacteria causing nosocomial infections was done by several tests in Jayapura Health Laboratory. The result showed that there were 15 aerobic bacteria consisted of: the coccid gram–positive bacteria: Staphylococus epidermidis, S. aureus, S. saprophyticus, Streptcoccus sp; the rod gram-positive bacteria: Listeria monocytogenes, Diptheroid sp, Lactobacillus sp and gram-negative bacteria: Providence rettgeri, Pseudomonas puttrefaciens, Klebsiela ozaena, P. malthophyla, Morganela morganii, Serattia sp, K. oxytoca, and K. pneumonia. Key words: Aerobic bacteria, identification of bacteria, nosocomial infection, surgical room, Jayapura.

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Penapisan Bakteri Filosfer Penghasil Senyawa Bioaktif Anti Xanthomonas oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri pada Padi

Jurnal Sumberdaya Hayati ◽

10.29244/jsdh.2.1.19-24 ◽

2016 ◽

Vol 2 (1) ◽

pp. 19-24

Author(s):

NURFITRIANI RINA ◽

NI PUTU RATNA AYU KRISHANTI ◽

ALINA AKHDIYA ◽

ARIS TRI WAHYUDI

Keyword(s):

Bioactive Compounds ◽

Pathogenic Bacteria ◽

Luria Bertani ◽

Xanthomonas Oryzae ◽

Growth Stages ◽

Pathogenicity Test ◽

Bacterial Isolates ◽

Gram Positive ◽

Gram Positive Bacteria ◽

Phyllosphere Bacteria

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the important diseases in rice crops in Indonesia. The disease is difficult to be controlled since it attacks the rice plant at different growth stages such as tillering, flowering and ripening. One of the alternatives that could be used to control the disease is by using phyllosphere bacteria as the biocontrol agents. This study aims to isolate, characterize and screen the rice phyllosphere bacteria producing bioactive compounds against Xoo. Phyllosphere bacteria isolated from healthy leaves of rice var. Ciherang by using 4 different media obtained 285 bacterial isolates which were consisted of the 65 isolates of King’s B agar, 86 isolates of Nutrient agar, 81 isolates of Luria-Bertani agar, and 53 isolates of Trypticase Soy agar media. Antagonist test using double layer method showed 58 isolates of phyllosphere bacteria produced bioactive compounds that inhibited the growth of Xoo. Pathogenicity test agaist rice leaf revealed 18 bacterial isolates did not perform their potencies as pathogenic bacteria. Among the 18 non-phytopathogenic bacterial isolates, 14 isolates belong to Gram-positive bacteria and 4 isolates belong to Gram-negative bacteria. Five isolates among Gram positive bacteria were predicted as Bacillus genera.

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