Chemical profile, antimicrobial activity, toxicity on Artemia salina and anti-acetylcholinesterase enzyme essential oil from Bauhinia ungulata L. (Fabaceae) leaves

The present study aimed to evaluate the chemical composition, antioxidant potential, and the cytotoxic and antimicrobial activity of the essential oil of the plant species Tithonia diversifolia (Hemsl) A. Gray. The essential oil obtained was used to identify the chemical compounds present through the techniques of GC-MS and NMR. The antioxidant potential was calculated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl. For cytotoxic activity, the larval mortality of Artemia salina was evaluated. The main chemical constituents identified are αpinene (9.9%), Limonene (5.40%), (Z)-β-ocimene (4.02%), p-cymen-8-ol (3.0%), Piperitone (11.72%), (E)-nerolidol (3.78%) and Spathulenol (10.8%). In the evaluation of the antimicrobial activity, bacterial strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used. The results showed that the bacterium E. coli were more susceptible to the presence of the essential oil, presenting minimal inhibitory concentration at the concentrations that were exposed. The essential oil presented antioxidant activity of 54.6% at the concentration of 5 mg·mL−1 and provided a CI50 of 4.30. It was observed that the essential oil of this species was highly toxic against A. salina lavas, as its cytotoxic activity showed an LC50 of 3.11. Thus, it is concluded that T. diversifolia oils are effective in inhibiting bacterial growth and reducing oxidative stress.

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Chemical profile and antimicrobial activity of essential oil and methanol extract from peels of four Durio zibethinus L. varieties

Biomass Conversion and Biorefinery ◽

10.1007/s13399-021-02134-0 ◽

2022 ◽

Author(s):

Nor Hisam Zamakshshari ◽

Idris Adewale Ahmed ◽

Nur Alyaa Mat Didik ◽

Muhammad Nazil Afiq Nasharuddin ◽

Najihah Mohd Hashim ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Methanol Extract ◽

Chemical Profile ◽

Durio Zibethinus

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Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

10.1101/595900 ◽

2019 ◽

Cited By ~ 1

Author(s):

Renata do Socorro Barbosa Chaves ◽

Rosany Lopes Martins ◽

Alex Bruno Lobato Rodrigues ◽

Érica de Menezes Rabelo ◽

Ana Luzia Ferreira Farias ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Artemia Salina ◽

High Cytotoxic Activity ◽

Origanum Majorana ◽

Main Component

ABSTRACTThis study evaluated the larvicidal activity of O. majorana essential oil, identified the chemical composition, evaluated the antimicrobial, cytotoxic and antioxidant potential. The larvicidal activity was evaluated against larvae of the third stage of Aedes aegypti, whereas the chemical composition was identified by gas chromatography coupled to mass spectrometer, the antimicrobial activity was carried out against the bacteria Pseudomonas aeruginosa, Escherichia coli and Staphylococcus auereus, the antioxidant activity was evaluated from of 2.2-diphenyl-1-picryl-hydrazila sequestration and Artemia salina cytotoxicity. Regarding to the results, the larvicidal activity showed that O. majorana essential oil caused high mortality in A. aegypti larvae. In the chromatographic analysis, the main component found in O. majorana essential oil was pulegone (57.05%), followed by the other components verbenone (16.92%), trans-p-menthan-2-one (8.57%), iso-menthone (5.58%), piperitone (2.83%), 3-octanol (2.35%) and isopulegol (1.47%). The antimicrobial activity showed that E. coli and P. aeruginosa bacteria were more sensitive to oil than S. aureus, which was resistant at all concentrations. Essential oil did not present antioxidant activity, but it has high cytotoxic activity against A. salina.

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Antimicrobial activity of the essential oil from Bixa orellana L.

Research Society and Development ◽

10.33448/rsd-v9i10.8447 ◽

2020 ◽

Vol 9 (10) ◽

pp. e8129108447

Author(s):

Yuri Nascimento Fróes ◽

Ana Patrícia Matos Pereira ◽

Paulo Victor Serra Rosa ◽

Laiane Araujo da Silva Souto ◽

Mariana Oliveira Arruda ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Total Phenolics ◽

Chemical Characterization ◽

Artemia Salina ◽

Bixa Orellana ◽

Toxicity Assay ◽

Natural Substances ◽

Application Potential ◽

And Staphylococcus Aureus

The application of natural substances with bactericidal action is the target of research aimed at a more sustainable production, implying in various sources of substances for new therapeutic formulations. This study presents the chemical characterization, toxicity and antimicrobial activity of essential oil (EO) of Bixa orellana Labill leaves. EO was extracted by hydrodistillation at 100°C for 3h. Physicochemical parameters were determined and chemical characterization used Gas chromatography coupled with Mass Spectrometry (GC/MS). The toxicity assay was performed through the bioassay with Artemia salina Leach. The methods of Disc Diffusion and Dilution in Broth in front of the bacteria Escherichia coli and Staphylococcus aureus were used to evaluate the antimicrobial activity. LC50 in the toxicity assay was verified in 355 mg L-1 and was classified as nontoxic. The OE presented antimicrobial activity against the microorganisms tested and totaled 320 mg EAT g-1 of total phenolics. The results obtained highlight the importance of pointing out this product as a therapeutic alternative, encouraging its application potential.

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Chemical profile and antioxidant, antibacterial, and cytotoxic activities on Artemia salina from the essential oil of leaves and xylopodium of Cochlospermum regium

Scientific Electronic Archives ◽

10.36560/15120221506 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Roberta Heloísa de Paula Magalhães ◽

Antonio Carlos Pereira de Menezes Filho ◽

Matheus Vinicius Abadia Ventura ◽

Hellen Regina Fernandes Batista-Ventura ◽

Carlos Frederico de Souza Castro ◽

...

Keyword(s):

Antioxidant Activity ◽

Refractive Index ◽

Essential Oil ◽

Relative Density ◽

Optical Rotation ◽

Artemia Salina ◽

Cytotoxic Activities ◽

Chemical Profile ◽

Radical Reduction ◽

Antibacterial Potential

Cochlospermum regium is a shrub plant species from the Cerrado domain used in traditional medicine. This study aimed to evaluate the chemical profile and antioxidant, antibacterial and cytotoxic activities on Artemia salina from the essential oil of fresh leaves and xylopodium. Fresch leaves and xylopodium of C. regium were collected in an area of Cerrado domain in Goiás State, Brazil, 2021. The essential oil was obtained by hydrodistillation, the yield was quantified and the chemical profile determined by gas chromatography with mass spectrometry (GC-MS). Physicochemical analyzes were carried out for organoleptic analysis (color and appearance), solubility, relative density (g mL-1), refractive index, optical rotation (αD), antioxidant activity in DPPH radical reduction (IC50 µL mL-1), antibacterial activity on Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Salmonella serovar Enteritidis and Salmonella serovar Thyphymurium by the disc method (mm), and cytotoxicity bioassay on Artemia salina (LC50 µg mL-1). The major compounds for the essential oil of fresh leaves were viridiflorol 10.21%, Copaen-4-α-ol <β>, longiborneol 9.07 and β-bisabolene 11.48%, and for the essential oil of xylopodium β-selinene 26.17%, aromadendrene 8.66 % and thujopsene 8.09%. The yield was 0.58 and 0.33%, color slightly yellow and yellow for fresh leaves and xylopodium, respectively. Positive solubility, refractive index of 1.3468 and 1.3347, optical rotation +48.8 and +21.5, relative density 0.932 and 0.936 g mL-1, antioxidant activity IC50 = 47.65 and 111.16 µL mL-1 for fresh leaves essential oil and xylopodium, respectively. The essential oil from fresh leaves showed high antibacterial potential for all strains, as well as for cytotoxic activity on A. salina with LC50 = 90.17 and 625.08 µg mL-1, respectively.

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Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity

Molecules ◽

10.3390/molecules25173943 ◽

2020 ◽

Vol 25 (17) ◽

pp. 3943 ◽

Cited By ~ 2

Author(s):

Valtcho D. Zheljazkov ◽

Vladimir Sikora ◽

Ivanka B. Semerdjieva ◽

Miroslava Kačániová ◽

Tess Astatkie ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Essential Oil ◽

Cannabis Sativa ◽

Caryophyllene Oxide ◽

Chemical Profile ◽

Processing Industry ◽

Germacrene D ◽

Pharmaceutical Industries ◽

Ground Material

The hypothesis of this study was that we can modify the essential oil (EO) profile of hemp (Cannabis sativa L.) and obtain fractions with differential composition and antimicrobial activity. Therefore, the objective was to evaluate the effects of grinding of hemp biomass before EO extraction and fractionation during distillation on EO profile and antimicrobial activity. The study generated a several EO fractions with a diversity of chemical profile and antimicrobial activity. The highest concentrations of β-pinene and myrcene in the EO can be obtained in the 5–10 min distillation time (DT) of ground material or in the 80–120 min DT of nonground material. High δ-3-carene and limonene EO can be obtained from 0–5 min DT fraction of nonground material. High eucalyptol EO can be sampled either in the 0–5 min DT of the ground material or in the 80–120 min of nonground material. Overall, the highest concentrations of β-caryophyllene, α-(E)-bergamotene, (Z)-β-farnesene, α-humulene, caryophyllenyl alcohol, germacrene D-4-ol, spathulenol, caryophyllene oxide, humulene epoxide 2, β-bisabolol, α-bisabolol, sesquiterpenes, and cannabidiol (CBD) can be obtained when EO is sampled in the 80–120 min DT and the material is nonground. Monoterpenes in the hemp EO can be increased twofold to 85% by grinding the material prior to distillation and collecting the EO in the first 10 min. However, grinding resulted in a slight but significant decrease in the CBD concentration of the EO. CBD-rich oil can be produced by collecting at 120–180 min DT. Different EO fractions had differential antimicrobial activity. The highest antimicrobial activity of EO fraction was found against Staphylococcus aureus subsp. aureus. THC-free EO can be obtained if the EO distillation is limited to 120 min. The results can be utilized by the hemp processing industry and by companies developing new hemp EO-infused products, including perfumery, cosmetics, dietary supplements, food, and pharmaceutical industries.

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Evaluation of the Larvicidal Potential of the Essential Oil Pogostemon cablin (Blanco) Benth in the Control of Aedes aegypti

Pharmaceuticals ◽

10.3390/ph12020053 ◽

2019 ◽

Vol 12 (2) ◽

pp. 53 ◽

Cited By ~ 1

Author(s):

Lizandra Lima Santos ◽

Lethicia Barreto Brandão ◽

Rosany Lopes Martins ◽

Erica de Menezes Rabelo ◽

Alex Lobato Rodrigues ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Chemical Constituents ◽

Chemical Characterization ◽

Artemia Salina ◽

World Health ◽

Pogostemon Cablin

The objective of this work was to collect information on the chemical constituents that demonstrate the larvicidal activity against Aedes aegypti, as well as the antioxidant, microbiological, and cytotoxicity potential of the essential oil of Pogostemon cablin leaves. The chemical characterization was performed by gas chromatography coupled to mass spectrometer (GC-MS). The larvicidal activity was performed according to the protocol of the World Health Organization. The antioxidant activity was evaluated through the sequestering capacity of 2,2-diphenyl-1-picryl-hydrazine (DPPH). As for the microbiological evaluation, the microdilution technique was used, according to the protocol of the Clinical and Laboratory Standards Institute. The cytotoxic activity was evaluated against the larvae of Artemia salina. The species P. cablin presented the following compounds: Patchouli alcohol (33.25%), Seyshellene (6.12%), α-bulnesene (4.11%), Pogostol (6.33%), and Norpatchoulenol (5.72%), which was in synergy with the other substances may significantly potentiate the larvicidal action of the species with the LC50 of 28.43 μg·mL−1. There was no antioxidant activity, however, it presented antimicrobial activity against all bacteria tested with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of 62.5 μg·mL−1. The species demonstrated significant toxic action with LC50 of 24.25 μg·mL−1. Therefore, the P. cablin species showed significant larvicidal potential, antimicrobial activity, the absence of antioxidant action, and high toxicity.

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Chemical profile and antimicrobial potential of essential oils of Cymbopogon citratus (DC.) Stapf, Ocimum basilicum Linn and Aniba rosaeodora Ducke

Scientia Plena ◽

10.14808/sci.plena.2020.061502 ◽

2020 ◽

Vol 16 (6) ◽

Author(s):

Gustavo Oliveira Everton ◽

Paulo Sérgio Santos Júnior ◽

Rebecca Jemima Pereira Araújo ◽

Aline Medeiro Ferreira ◽

Paulo Roberto Barros Gomes ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Physicochemical Parameters ◽

Artemia Salina ◽

Chemical Profile ◽

E Coli ◽

Diffusion Technique ◽

Mueller Hinton ◽

Major Chemical

The study evaluated the chemical profile, antimicrobial activity and toxicity of essential oils (EOs) of C. citratus, O. basilicum and A. rosaeodora. The EOs were extracted by hydrodistillation, the chemical profile was determined using the physicochemical parameters and the chemical composition was obtained by gas chromatography coupled to mass spectrometry (GC-MS). The toxicity assay followed the Artemia salina Leach bioassay. To perform the antimicrobial activity, the Disc Diffusion technique and the Mueller Hinton Broth Dilution (MH) technique were used. The physicochemical parameters of the EOs showed satisfactory results. The major chemical composition of the EO of A. rosaeodora presented linalool (93.60%), the EO of C. citratus exhibited geranial (43.96%) and EO of O. basilicum found methyl chavicol. All EOs in this study had LC50 between 582 mg L-1 and 282 mg L-1. The antimicrobial activity of EO C. citratus demonstrated inhibition halos for S. aureus of 25 mm and 25 mm for E. coli, EO of O. basilicum exhibited inhibition halo of 18 mm for E.coli and 20 mm for S. aureus and EO of A. rosaeodora presented 11 mm for E. coli and 15 for S. aureus. As the values of MIC and MBC found, the EO of O. basilicum presented a more effective inhibitory action against S. aureus. It was concluded that the observed biological potentials encourage the application potentials in the efficiency of the control of pathogenic microorganisms.

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