Effect of Aqueous Extract on Total Phenolic Content and Antimitotic Activity of Ficus Benghalensis root on Allium Cepa Root Meristamatic cells

Eragrostis plana é uma das espécies alóctones com maior potencial invasivo nas áreas de pastagem natural do Sul da América do Sul, devido suas características biológicas e ecológicas. Os objetivos desse trabalho foram quantificar os fenóis totais presentes no extrato aquoso de extratos de parte aérea incubada de E. plana e avaliar o seu efeito na germinação e no desenvolvimento de plântulas de três espécies cultivadas. A quantidade total de fenóis presentes no extrato aquoso da biomassa degradada foi de aproximadamente 0,75 g L-1, o que equivale a 1,35% da massa seca da parte aérea das plantas. A alfafa foi a espécie com maior sensibilidade ao estrato aquoso, tendo tanto a germinação quanto o desenvolvimento de plântula inibidos. Braquiária brizanta e trigo sofreram apenas no desenvolvimento das plântulas. Braquiária brizanta foi a única espécie que se beneficiou do extrato aquoso, apresentando menor número de plântulas anormais. Mais estudos são necessários para caracterizar os aleloquímicos presentes no extrato aquoso da fitomassa de E. plana, sua taxa de liberação e seu comportamento no solo de áreas infestadas.

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Investigation on Green Synthesis, Biocompatibility, and Antibacterial Activity of Silver Nanoparticles Prepared Using Cistus incanus

Materials ◽

10.3390/ma14175028 ◽

2021 ◽

Vol 14 (17) ◽

pp. 5028

Author(s):

Wioletta Florkiewicz ◽

Klaudia Pluta ◽

Dagmara Malina ◽

Karolina Rudnicka ◽

Anna Żywicka ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antioxidant Capacity ◽

Gallic Acid ◽

Aqueous Extract ◽

Total Phenolic Content ◽

Phenolic Content ◽

Dry Weight ◽

Total Phenolic ◽

Composition Analysis ◽

Cistus Incanus

This paper describes the plant-mediated preparation of silver nanoparticles with aqueous extract and infusion of Cistus incanus leaves. To evaluate aqueous extract and infusion antioxidant capacity and total phenolic content the DPPH and Folin–Ciocalteau methods were utilized. The antioxidant capacity and total phenolic content of extract and infusion were equal to 85.97 ± 6.54 mg gallic acid equivalents per gram of dry weight.; 10.76 ± 0.59 mg/mL and 12.65 ± 1.04 mg gallic acid equivalents per gram of dry weight.; 3.10 ± 0.14 mg/mL, respectively. The formed nanoparticles displayed the characteristic absorption band in the 380–450 nm wavelength range. The average size of particles was in the 68.8–71.2 nm range. Morphology and phase composition analysis revealed the formation of spherical nanoparticles with a face-centred cubic structure. Immune compatibility tests of nanoparticles and plant extracts showed no activation of the THP1-XBlue™ monocyte. Cytotoxicity tests performed with L929 mice fibroblasts showed that nanoparticles should be utilized at a concentration of 16 ppm. The minimum inhibitory concentrations determined with the microdilution method for nanoparticles prepared with plant infusion for S. aureus and S. epidermidis were 2 ppm and 16 ppm, respectively.

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Evaluation of total phenolic content and antioxidant activities from different type of extraction technique of Helianthus tuberosus

Advances in Agricultural and Food Research Journal ◽

10.36877/aafrj.a0000165 ◽

2020 ◽

Author(s):

Nur Diyana Alyas ◽

Nurin Izzati Mohd Zulkifli ◽

Noor Zafira Noor Hasnan

Keyword(s):

High Pressure ◽

Aqueous Extract ◽

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Jerusalem Artichoke ◽

Total Phenolic ◽

Helianthus Tuberosus ◽

Extraction Techniques ◽

Pressure Steam

This study investigated the difference of antioxidants activity from different extraction techniques of Jerusalem artichoke (Helianthus tuberosus). Three different extraction techniques involved were aqueous extraction, high-pressure steam extraction and 70% ethanol extraction. Total phenolic content (TPC) was determined using the Folin-Ciocalteu method. The antioxidant activities were analysed using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The high-pressure steam extract showed the highest total phenolic content and both FRAP and DPPH activities at 127.87 ± 0.08 mg GAE/g, 53.38 ± 2.76 mg AAE/g and 79.92 ± 0.26 mg AAE/g. Meanwhile, the aqueous extract showed 2-fold higher of total phenolic content compared to ethanol extract at 65.73 ± 9.44 and 30.61 ± 0.00 mg GAE/g. Conversely, antioxidant activities obtained from ethanol extracts (41.49 ± 2.54 and 41.10 ± 0.10 mg AAE/g.) were higher compared to aqueous extract (23.29 ± 0.47 and 5.24 ± 0.38 mg AAE/g.). This study suggests that different extraction methods influenced the findings of total phenolic content and antioxidant activities of the Jerusalem artichoke. Hence, Jerusalem artichoke can be considered as a potential source of natural antioxidants.

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A Comparative Study on Phytochemical Screening and Antioxidant Activity of Aqueous Extract from Various Parts of Moringa oleifera

Indonesian Journal of Natural Pigments ◽

10.33479/ijnp.2021.03.2.43 ◽

2021 ◽

Vol 3 (2) ◽

pp. 43

Author(s):

Awalul Fatiqin ◽

Hanif Amrulloh ◽

Ike Apriani ◽

Aneke Lestari ◽

Berta Erawanti ◽

...

Keyword(s):

Antioxidant Activity ◽

Aqueous Extract ◽

Total Phenolic Content ◽

Moringa Oleifera ◽

Phenolic Content ◽

Total Phenolic ◽

Phytochemical Screening ◽

Flavonoid Content ◽

Leaf Part ◽

Different Parts

In this work, we reported a comparative study on the phytochemical screening and antioxidant activity of aqueous extract from different parts of Moringa oleifera plant, i.e. leaves, twigs, stem barks, and woods. The phytochemical content of each extract was screened through qualitative analysis and followed by quantitative analysis for the determination of total phenolic content and total flavonoid content. The results showed that different parts of Moringa oleifera plant would give different extracted natural products. Either leaf or twig part of Moringa oleifera contained alkaloids, flavonoids, saponins, carbohydrates, polyphenols, proteins, amino acids, and phenolics. Meanwhile, only flavonoids, carbohydrates, and phenolics were found in the aqueous extract of the wood part of Moringa oleifera. In agreement to the phytochemical assay, the aqueous extract of leaf part of Moringa oleifera gave the highest total phenolic content (684 ± 16.7 µg GAE mL-1) and the highest flavonoid content (514 ± 26.1 µg CE mL-1) than other parts. Consequently, the leaf extract exhibited the highest antioxidant activity (64.6 ± 0.69%) which is close to the antioxidant activity of ascorbic acid (71.7 ± 0.56%) as the positive control. This finding is critical for the utilization of Moringa oleifera leaf part as the most potent source of antioxidant agents in the future.

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The antimicrobial activity of Prunella vulgaris extracts

Herba Polonica ◽

10.1515/hepo-2015-0008 ◽

2015 ◽

Vol 61 (1) ◽

pp. 31-38 ◽

Cited By ~ 1

Author(s):

Mohaddese Mahboubi ◽

Atefeh Mahboubi ◽

Nastaran Kazempour

Keyword(s):

Antimicrobial Activity ◽

Aqueous Extract ◽

Total Phenolic Content ◽

Methanol Extract ◽

Phenolic Content ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Total Phenolic ◽

Prunella Vulgaris ◽

Ethanol Extracts

Summary Prunella vulgaris ( Labiatae family) or self-heal is traditionally used for different ailments such as eye pain and inflammation, headache, dizziness, sore throat and wound healing. Total phenolic and total flavonoid contents of extracts (methanol, ethanol and aqueous) were determined by a spectrophotometer. The antimicrobial activity was evaluated by micro broth dilution assay. The total phenolic content of P. vulgaris extracts were higher in aqueous extract (156.5 mg GAC/g) followed by ethanol extract and methanol extract. The TFC content of P. vulgaris methanol extract (82.8 mg QE/g) was higher than ethanol extract (22.7 mg QE/g) and aqueous extract (16.2 mg QE/g). The antimicrobial activity of methanol or ethanol extracts was higher than aqueous extract from P. vulgaris. The sensitivity of microorganisms to different extracts is related to type of pathogens. There is no positive relation between total phenolic content and its antimicrobial activity. Prunella vulgaris ethanolic extract as a source of phenolic and flavonoid contents can be used as an antimicrobial agent.

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Total Phenolic Content, Antioxidant, Cytotoxicity and Hepatoprotective Activities of Aqueous Extract of Channa striatus (Haruan)

IOSR Journal of Nursing and health Science ◽

10.9790/1959-03635259 ◽

2014 ◽

Vol 3 (6) ◽

pp. 52-59 ◽

Cited By ~ 1

Author(s):

Hazirah Abdul Radzak ◽

◽

Abdah Md Akim ◽

Siti Sarah Sazali ◽

Zainal Baharum ◽

...

Keyword(s):

Aqueous Extract ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Channa Striatus

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Pharmacognostic evaluation, estimation of phenolic, ϐlavonoid composition and antioxidant activity of Aspidopterys indica (Willd.)W.Theob: An endemic plant to peninsular India

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v12i2.4661 ◽

2021 ◽

Vol 12 (2) ◽

pp. 1223-1232

Author(s):

Udaya Chandrika P ◽

Sunitha K

Keyword(s):

Aqueous Extract ◽

Total Phenolic Content ◽

Phenolic Content ◽

Methanolic Extract ◽

Total Phenolic ◽

Flavonoid Content ◽

Endemic Plant ◽

Peninsular India ◽

Crude Fiber Content ◽

Dpph Scavenging

Aspidopterys indica (Willd.)W.Theob is endemic plant to Peninsular India belonging to family Malpighiaceae commonly known as Chuttakulaa-tigga. The study aims to explore Pharmacognostic, Phytochemical evaluation, Total Phenolic, Flavonoid Content and Antioxidant Activities.The Pharmacognostic study comprises of Ultrasonic extraction, microscopy of Leaf and stem, fluorescence investigation, ash values, extractive values, Loss on drying, foaming index, crude fiber content, total phenolic and flavonoid content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and Nitric oxide scavenging activities were assessed.The leaf shows presence of paracytic stomata, T shaped trichomes, Stem shows presence of vessels and pith. The physicochemical characters assessed as Total Ash value 10%, Water insoluble ash 0.5%, acid insoluble ash 8.5%, sulphated ash 4.2%, alcoholic extractive value 16.8%, water soluble extractive value 17.6%, chloroform extractive value 0.8%, Loss on drying 3.36%, Foaming index is <100, crude fiber content is 2.4%, phytoconstituents like tannins, flavonoids, phytosterols and alkaloids, carbohydrates are available in methanol, aqueous, chloroform extracts. Total phenolic content turned greatest in methanolic extract (90.69±2.86 mg GAE/g), Aqueous extract showed maximum total flavonoid content (109.89±0.33mg of QE/g). Methanolic extract indicated most intense 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging action IC50(134.97±8.37) whereas aqueous extract is potent to exclude nitrile radical IC50108.00±4.18). Total phenolic content - DPPH scavenging assay are positively correlated. The microscopical, physicochemical parameters are valuable to authenticate the identification and purity of the drug. A.indica is a perspective source of antioxidants.

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Toxicological properties of an aqueous extract of Aristolochia triangularis leaves, using the brine shrimp lethality and Allium cepa bioassays

Ciência Rural ◽

10.1590/0103-8478cr20190091 ◽

2019 ◽

Vol 49 (8) ◽

Author(s):

José Darlan Alves da Silva ◽

Cláudio Rodrigo Nogueira ◽

Maria do Carmo Vieira ◽

Silvia Cristina Heredia-Vieira ◽

Alexeia Barufatti ◽

...

Keyword(s):

Antioxidant Activity ◽

Allium Cepa ◽

Aqueous Extract ◽

Brine Shrimp ◽

Phenolic Content ◽

Artemia Salina ◽

Total Phenolic ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power ◽

Beneficial Property

ABSTRACT: The aqueous extract of Aristolochia triangularis leaves showed activity against Artemia salina larvae, with an LD50 of 370.6 µg/mL. In experiments with Allium cepa seeds, the extract caused a 51.26% reduction in the germination index, inhibited mean root growth, and was cytotoxic at concentrations of 668 and 2,000 µg/mL. Its antioxidant activity was additionally assessed in this research and the ferric reducing antioxidant power value was found to be 391.2 µM/g. Such health-beneficial property can be attributed partly to the total phenolic content, spectrophotometrically determined as 52.67 mg/g. The occurrence of cytotoxicity suggested caution when consuming teas from A. triangularis leaves for medicinal purposes, and equally reveals the need for further studies to investigate their adverse effects.

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Penetapan Kadar Fenolik Total Ekstrak Kacang Panjang (Vigna unguiculata) dengan Metode Spektrofotometri UV-Visible

Lumbung Farmasi: Jurnal Ilmu Kefarmasian ◽

10.31764/lf.v2i1.3759 ◽

2021 ◽

Vol 2 (1) ◽

pp. 102

Author(s):

Lalu Aang Robby Dewantara ◽

Agus Dwi Ananto ◽

Yayuk Andayani

Keyword(s):

Gallic Acid ◽

Aqueous Extract ◽

Vigna Unguiculata ◽

Total Phenolic Content ◽

Phenolic Content ◽

Ethanolic Extract ◽

Antioxidant Potential ◽

Total Phenolic ◽

Uv Visible ◽

Gallic Acid Equivalent

ABSTRAKKacang panjang (Vigna unguiculata) merupakan salah satu tanaman yang berpotensi sebagai antioksidan karena mengandung metabolit sekunder hampir di seluruh bagian tanamannya, seperti pada daging buahnya. Penelitian ini bertujuan untuk menentukan kadar fenolik total ekstrak etanol dan ekstrak air daging buah kacang panjang dengan metode spektrofotometri UV-Visible. Sampel daging buah kacang panjang dimaserasi menggunakan dua pelarut berbeda, yaitu pelarut etanol 96% dan pelarut air. Analisis kadar fenolik total dilakukan dengan metode spektrofotometri UV-Visible. Kadar fenolik total dinyatakan dalam gallic acid equivalent (GAE). Hasil analisis menunjukkan bahwa kadar fenolik total ekstrak etanol kacang panjang sebesar 40,4173 ± 4.2303 mg GAE/g dan kadar fenolik total ekstrak air kacang panjang sebesar 60.6062 ± 2,8533 mg GAE/g. Ekstrak etanol dan ekstrak air dari daging buah kacang panjang berpotensi sebagai antioksidan dilihat dari parameter kadar fenolik totalnya. Berdasarkan hasil penelitian, diperoleh bahwa kadar fenolik total ekstrak air lebih tinggi dibandingkan ekstrak etanol daging buah kacang panjang. Kata kunci : Kacang panjang (Vigna unguiculata); Spektrofotometri UV-Visible; Kadar fenolik total.ABSTRACTCowpea (Vigna unguiculata) is one of the vegetables that have antioxidant potential due to it contains some secondary metabolites that scattered on every part of the Cowpea, such as the pod coat. The aimed of this study was to determine total phenolic content of Cowpea pod coat ethanolic extract and aqueous extract using UV-Vis spectrophotometry method. Cowpea pod coats were macerated using two different solvents, ethanol 96%, and aqueous. Total phenolic content was showed as gallic acid equivalent (GAE). The analysis results showed that total phenolic content of ethanol extract of cowpea was 40,41732 ± 4.2303 mg GAE/g and total phenolic content of aqueous extract of cowpea was 60.6062 ± 2,8533 mg GAE/g. The ethanolic extract and aqueous extract of cowpea pod coat have antioxidant potential in terms of total phenolic content parameter. Based on the research, the total phenolic content of cowpea pod aqueous extract higher than cowpea pod coat ethanolic extract.Keywords : Cowpea (Vigna unguiculata); Spectrophotometric UV-Visible; Total phenolic content.

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