scholarly journals UJI IN VIVO TAHAP PREKLINIS TERHADAP EKSTRAK BATANG PISANG (Musa paradisiaca L.) SEBAGAI ANTIINFLAMASI TOPIKAL

2021 ◽  
Vol 3 (2) ◽  
pp. 138-151
Author(s):  
Ni Luh Kade Arman Anita Dewi ◽  
Putu Era Sandhi Kusuma Yuda ◽  
I Gede Agus Suarnata ◽  
Maria Malida Vernandes Sasadara
Keyword(s):  
Tissue Injury ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Musa Paradisiaca ◽  
Stem Extract ◽  
Anti Inflammatory ◽  
Banana Stem ◽  
Inflammatory Effect

Inflammation is a normal protective response to tissue injury caused by physical trauma, chemical damage, or microbiological substances. Banana stems (Musa paradisiaca L.) have been empirically believed for their topical anti-inflammatory properties. The purpose of this study was to scientifically evaluate the in vivo preclinical anti-inflammatory activity of banana stems. This research was designed experimentally using twenty-five albino male rats which were divided into five groups: the positive control group which received sodium diclofenac gel, the negative control group which received gel basis preparation without any active ingredient, and three experimental groups which received banana stem extract gel in three different concentrations, 5%, 10%, and 15%. Every five rats of every group were firstly injected with 1% of carrageenan, and the anti-inflammatory effect was evaluated every hour for 4 hours using a plethysmometer.  The collected data were statistically evaluated. The results showed that banana stem extract gel with concentrations of 15% produced the best anti-inflammatory effect, compared to other gel concentrations. In conclusion, the preparation of 15% banana stem extract gel can be applied as potential topical anti-inflammatory preparation.

scholarly journals THE EFFECTS OF BANANA STEM (Musa paradisiaca var. sapientum) EXTRACT ON HISTOPATHOLOGIC GASTRIC OF RATS INDUCED BY INDOMETACHIN

2017 ◽  
Vol 52 (4) ◽  
pp. 246
Author(s):  
Rochmah Kurnijasanti ◽  
Arninda Alfiani Putri
Keyword(s):  
Qualitative Data ◽  
Corn Oil ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Histopathological Changes ◽  
Musa Paradisiaca ◽  
Stem Extract ◽  
Mucosa Epithelium ◽  
Banana Stem

This research aims to find out the administration effect of Ambon banana stem extract (Musa paradisiaca var. sapientum) to prevent gastric damage and observe histopathology of rat’s gastric induced by indomethacin. This research used 30 male rats age 8-12 week having 150 gram weight. They were selected randomly and divided into five groups. Negative control (K-) was given 0.5 ml CMC Na 0.5 % for 9 days and 0.5 ml corn oil was given on 10th. Positive control (K+) was given 0.5 ml CMC Na 0.5% for 9 days and then given Indomethacin emultion 5 mg/0.5 ml/150 g BW once on 10th day, and the other groups were given Ambon banana stem extract for (P1) 20 mg/150 g BW, (P2) 40 mg/150 g BW and (P3) 80 mg/150 g BW for 9 days. Afterwards, they were given Indometachin emultion 5 mg/150 g BW once on 10th day. Each gastric specimen was processed and histopathological changes were observed. Scoring of mucosa epithelium erosion and hemorrhagic, as qualitative data, was analyzed by Kruskall-Wallis test and continued by using Z test. The result shows that Pisang Ambon’s stem extract reduce significantly in gastric mucosa epithelium erosion and hemorrhagic induced by Indomethacin (p<0.05).

scholarly journals AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL 70% DAUN ASHITABA (Angelica keiskei) SECARA IN VIVO DENGAN PENGINDUKSI KARAGENAN

2019 ◽  
Author(s):  
Haka As'ada ◽  
Yardi Saibi ◽  
Hendri Aldrat
Keyword(s):  
Dose Range ◽  
White Male ◽  
Ethanol Extract ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Male Rat ◽  
Control Group ◽  
Angelica Keiskei ◽  
Anti Inflammatory

Ashitaba leaves (Angelica keiskei) or also known as tommorow's leaf is plant that known to have various health benefit, one of them is as an anti-inflammatory activity. The anti-inflammatory activity of ashitaba leaves has been known through in vitro assays. This study aims to determine the anti-inflammatory activity of 70% ethanol extract of ashitaba leaves through in vivo assay. Anti-inflammatory activity was performed on white male rat of Sprague dawley strain with induction method of edema on rat's foot using 1% carrageenan 0.2 ml. Rats were divided into 5 groups. The negative control group was given a 0.5% Na-CMC suspension, a positive control group was given sodium diclofenac suspension of 5.14 mg / kgBW, and the test group was given 70% ethanol extract of ashitaba leaves at a dose of 1000; 2000; and 4000 mg / kgBW suspended in 0.5% Na-CMC. The results showed that in that dose range the 70% ethanol extract of ashitaba leaves had anti-inflammatory activity that did not depend on the dose. Percentage of edema of 70% ethanol extract of ashitaba leaves dose 1000; 2000; 4000 mg / kgBB was significantly different with negative control (p ≤ 0,05) and had percentage of edema inhibition respectively 83,95%, 79,01%, and 80,25%. The results of this study showed that 70% ethanol extract of ashitaba leaves have anti-inflammatory activity. Keywords: Ashitaba, Angelica keiskei, tommorow's leaf, anti-inflammatory, carrageenan.

In vitro antihemolytic effect, in vivo anti inflammatory and In vitro antioxidant activity of Anchusa azurea Mill

2021 ◽  
Vol 20 ◽  
Author(s):  
Boussoualim Naouel ◽  
Trabsa Hayat ◽  
Krache Imane ◽  
Ouhida Soraya ◽  
Arrar Lekhmissi ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Folk Medicine ◽  
Oxidative Degradation ◽  
Negative Control ◽  
Dependent Manner ◽  
Anti Inflammatory ◽  
Β Carotene ◽  
Inflammatory Effect

Background: Anchusa azurea Mill. (AA) is a medicinal plant largely used traditionally in folk medicine in Algeria, it is locally named: hamham. It is effective in the treatment of various diseases. Objectives: The aim of the present study is to determine the antioxidant, anti-inflammatory and anti-hemolytic effects of phenolic fractions from Anchusa azurea Mill. Methods: In this study, various extracts from Anchusa azurea Mill. (AA) using solvents with increasing polarity were prepared. The quantification of polyphenols and flavonoids was determined. The anti-radical activity of the different extracts was evaluated using DPPH and by measuring the inhibition of the oxidative degradation of β-carotene. The In vitro antihemolytic effect of the plant extracts is determined (CrE, ChE, AcE and AqE). For each extract, four concentrations were tested: 10.59, 21.18, 42.37, 84.74 µg/ml. Vitamin C is used as a standard. Free-radical attack was measured by measuring the HT50 (Half-Hemolysis Time). The anti-inflammatory effect using PMA on mice of the methanolic extract (CrE) was evaluated. Results: The quantification of polyphenols and flavonoids showed that ethyl acetate extract (AcE) contains a higher amount of polyphenols. However, chloroform extract (ChE) presents a higher amount of flavonoids. AcE showed an important scavenging activity using the DPPH radical (IC50= 68.35 µg/ml). The results showed that AcE also exhibited very great inhibition on the oxidation of β-carotene/linoleic acid (84.33%). All extracts increased the HT50 values (Half-Hemolysis Time) in a dose-dependent manner. The three highest concentrations (21.18, 42.37 and 84.74 µg / ml) of ChE caused a very significant delay (p ≤ 0.001) of hemolysis compared to the negative control and the positive control "VIT C". The anti-inflammatory effect using PMA on mice showed that the methanolic extract (CrE) of AA reduced the weight of the ear edema. Conclusions: This plant has a strong pharmacological power, which supports its traditional medicinal use.

scholarly journals Ujitoksisitasakut ekstrakbatangpisangambon(Musa paradisiacavar sapientum) terhadap hati mencit (Mus musculus) dengan parameter LD (Acute toxicity test of ambonese banana (Musa paradisiaca var sapientum) stem extract in liver of mice (Mus musculus) with LD 50 parameters 50

2014 ◽  
Vol 13 (2) ◽  
pp. 86
Author(s):  
Hendrik Setia Budi ◽  
Ira Arundina ◽  
Retno Indrawati ◽  
Leonita Widyana Mahardikasari
Keyword(s):  
Wound Healing ◽  
Mus Musculus ◽  
Toxicity Test ◽  
Control Group ◽  
Treatment Groups ◽  
Musa Paradisiaca ◽  
Significant Difference ◽  
Post Test ◽  
Stem Extract ◽  
Banana Stem

The sap of ambonese banana (Musa paradisiaca var sapientum) contain flavonoids, saponins, tannins which have beenwidely used by people in Trunyan Bali as traditional medicine on wound healing, and it has been reported as apotential wound healing after tooth extraction. The aim of this research was to determine the level of safety of usingherbal medicine, ambonese banana stem extract on histopatology liver of mice with LDparameters. This experimentalstudy was performed bythe post test only controlled group design. The sample were 28 mice (Mus musculus) randomlydivided into 4 groups. K group as control group was given aquadest. P1, P2, and P3, as treatment groups were givenambonese banana stem extract with dose 0.42g/20gbw, 2.1g/20gbw, 4.2g/20gbw. The extract was given per-oral withsonde on the first day. On day 3, the mice were terminated, and the livers were microscopically histopathological observed. The observed at 3th50 day, there were no deaths in every groups of mice (K, P1, P2, and P3) on the third day observation. Kruskal Wallis test showed there was not significant difference in histopathological appearance on liverof mice (p=0.771). It wasconcluded that the maximumsafety dose that can still be administered is 0.42g/20gbw followingthe LDparameter. Theacute toxicity test of ambonese banana stem extract did not show necrosis on liver but it showedthe highest simple degeneration than all groups which were given 0.42g/20gbw dose.

scholarly journals Antiinflammatory activity of 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates on carrageenan model of inflammation

2019 ◽  
pp. 82-87
Author(s):  
B. V. Pristupa ◽  
I. O. Shyshkin ◽  
Ya. V. Rozhkovsky ◽  
V. O. Gelmboldt
Keyword(s):  
Antiinflammatory Activity ◽  
Biologically Active ◽  
Inflammatory Activity ◽  
Male Rats ◽  
Control Group ◽  
Paw Edema ◽  
Commercial Preparation ◽  
Reference Drug ◽  
Anti Inflammatory ◽  
Inflammatory Effect

In the last decade, high cariesprophylactic efficacy of ammonium hexafluorosilicates, including salts with substituted biologically active ammonium cations, has been demonstrated. Among the promising anti-caries agents are recently synthesized 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates, for whose cations anti-inflammatory activity is expected. The aim of the work is to assess the presence of anti-inflammatory activity in the series of 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates in the experiment. 2-, 3-, 4-Carboxymethylpyridinium hexafluorosilicates (I–III, respectively) were synthesized according to the previously proposed technique, carrageenan is a commercial preparation, reference drug is a indomethacin. Experiments on the anti-inflammatory activity of hexafluorosilicates were performed on 77 white Wistar male rats weighing 174–190 g using the carrageenan model of inflammation. The inflammatory reaction was reproduced by subplantary administration of 0.1 ml of a 0.2% solution of carrageenan, the studied compounds were administered orally in doses 1/10, 1/20, 1/50 from LD50 for the compound III. It was established that after administration of carrageenan, animals of the control group showed marked paw edema, which gradually increased and was maximal after 24 hours of observation. According to the data obtained, the indices for compounds I-III practically do not differ from those of the control group. This indicates that, despite the results of the PASS forecast and the presence of acetic acid residue, an anti-inflammatory pharmacofor, in compounds I-III, the compounds under study do not have an anti-inflammatory effect in the carrageenan model. 2-, 3-, 4-Carboxymethylpyridinium hexafluorosilicates do not show the expected anti-inflammatory activity in the carrageenan model of inflammation.

scholarly journals Efektivitas ekstrak metanol batang pisang mauli 100%padapenyembuhan luka punggung mencit (Mus musculus)ditinjau dari jumlah sel radang (Effectiveness of 100%extract of Mauli Banana stem to healing process of mice’s (Mus musculus)back from the perspective of the number of inflamed cell)

2014 ◽  
Vol 13 (1) ◽  
pp. 33
Author(s):  
Maharani Laillyza Apriasari ◽  
Amy Nindia Carabelly ◽  
Gusti Febby Aprilia
Keyword(s):  
Mus Musculus ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Banana Plant ◽  
Significant Difference ◽  
Stem Extract ◽  
Banana Stem

Banana plant especially its trunk contains many kinds of fitokimia such as saponin, flavonoid, and tannin whichfunction as antibiotic and fasten the healing process. Mauli banana stem extract is often used by the people in HuluSungai Utara Banjarmasin Province to fasten healing skin injury. The purpose of this research is to histopathologicallyexamine the effectiveness of Mauli Banana stem extract to the healing process of the mice’s back (Mus musculus)onthe 3rd day. This purely true experimental research with post test only with control design used 27 mices which weredivided to 3 groups. Treatment group was given methanol extract of mauli banana stem whose average scores ofneutrofil and macrofag are representatively 2.333 and 1; negative control group was given aquadest with its averageneutrofil and macrofag scores are representatively 1.055 dan 1.678; and positive control group was given standardmedicine which contained Aloe veraextract with its average neutrofil and macrofag scores are representatively 1.066dan 1.667. The result of Kruskall-Wallis and Mann-Whiney showed a significant difference between treatment groupand negative control group. It can be concluded that the extract methanol of mauli banana stem on the healing processof injury to mancit’s back histopathologically influences the decreasing acute inflamed (neutrofil) cell and theincreasing chronic inflamed (macrophage) cell on the third day. In this case the extract of mauli banana stem ismedically potential to fasten the healing process of injury.

scholarly journals Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Asmaa Oubihi ◽  
Hanae Hosni ◽  
Issmail Nounah ◽  
Abdessamad Ettouil ◽  
Hicham Harhar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Female Rats ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Control Group ◽  
Standard Drug ◽  
Methanolic Extracts ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Thymus leptobotrys is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of Thymus leptobotrys aerial part. The yield of the methanolic extraction (22.2%) is higher than that of the ethanolic extraction (15.8%) and is characterized by higher contents of polyphenols 243.08 mg/g GAE (mg/g of gallic acid), flavonoids 179.28 mg/g RE (mg/g of rutin), and tannins 39.31 mg/g CE (mg/g of catechin). The in vitro measurement of antioxidant activity with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction test and Trolox equivalent antioxidant capacity (TEAC) test demonstrates the higher performance of the methanolic extract. The evaluation of the anti-inflammatory effect in vivo on adult Wistar female rats leads to a very significant decrease in the inflammation of the edema compared to the standard drug (indomethacin) and the control group. The toxicity test reveals that both extracts showed no toxicity within an LD50 above 2000 mg/kg body weight of the rats.

Genoprotective Effect of New Triazine Derivatives in Endosulfan Mediated Toxicity, an In vivo and In vitro Study

2018 ◽  
Vol 16 (1) ◽  
pp. 52-57
Author(s):  
Nima Naderi ◽  
Seyed Mostafa Ghasemi Najarkolaee ◽  
Mona Modanlookordi ◽  
Mohammad Shokrzadeh ◽  
Hamid Irannejad
Keyword(s):  
Neuroprotective Activity ◽  
Drug Candidate ◽  
Control Group ◽  
P Value ◽  
Amyloid Toxicity ◽  
Triazine Derivatives ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Background: Recently, we reported synthesis and neuroprotective activity of some new 1,2,4-triazine derivatives against H2O2 and &#946;-amyloid toxicity in two neurotic cell lines, SHSY5Y and PC12. Methods: The promising results obtained prompted us to further study on these potent neuroprotective agents. In the current study, in vivo anti-inflammatory effect and also genoprotective activity of these compounds in endosulfan-mediated toxicity were investigated. Compounds RT and SMO exhibited high anti-inflammatory effect at 3 and 4 hours after injection in 20 mg/kg, and were even more effective than Indomethacin (20 mg/kg). Results: Interestingly, compound SMO in 200 &#181;M was the best compound in reducing micronuclei significantly (P value <0.0001) in lymphocytes treated with endosulfan compared to control group. Conclusion: Herein, we report SMO as a genoprotective agent and a new drug candidate for endosulfan mediated toxicity.

Evaluation of in-feed larch sawdust anti-inflammatory effect in sows

2017 ◽  
Vol 20 (2) ◽  
pp. 321-327
Author(s):  
E.D. Tzika ◽  
P.D. Tassis ◽  
V.G. Papatsiros ◽  
E.M. Pferschy-Wenzig ◽  
A. Siochu ◽  
...  
Keyword(s):  
Corn Starch ◽  
Post Partum ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Lactation Period ◽  
Basic Diet ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract The study aimed to investigate the possible anti-inflammatory activity of larch sawdust as feed supplement in lactating sows’ diet and its possible effect on the prevalence of Postpartum Dysgalactia Syndrome under field conditions. In a Greek farrow-to-finish pig farm, fifteen sows were randomly and equally allocated to a negative control group (NC group), a positive control group (PC group), and a treatment group (LT group). The animals of the first two groups received 99% basic diet and 1% corn starch, while LT group animals received 99% basic diet and 1% larch sawdust. The whole trial period lasted 35 days (7 days prior to farrow – day of weaning). At parturition day, animals of the PC group received 2 ml of an anti-inflammatory drug intramuscularly (meloxicam, Metacam®, Boehringer Ingelheim Vetmedica), while the animals of both other groups, received 2 ml of normal saline. Results showed insignificant differences among experimental groups for parameters such as post-partum rectal temperature and piglets performance. On the contrary, a significant increase of mean milk lactation index was observed in LT and PC groups on the 4th day of lactation period, when compared with NC group (p=0.014). Additionally, mean IL-6 concentrations in blood in the LT group showed a tendency for reduction when compared with those found in NC, and insignificant difference (p>0.05) when compared with those observed in PC group 24 hours postpartum. Moreover, the respective TNFα mean level in the LT group at 24 and 72 hours after parturition was similar to that found in PC group, respectively) and significantly lower than that determined in the NC group (p=0.003, p=0.024. The results suggest a possible anti-inflammatory effect of larch sawdust in sows.

scholarly journals UJI AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL DAUN PISANG AMBON (Musa paradisiaca L.) TERHADAP TIKUS PUTIH (Rattus norvegicus L.) YANG DIINDUKSI KARAGENAN

2015 ◽  
Vol 1 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Sukmawati Sukmawati ◽  
Yuliet Yuliet ◽  
Ririen Hardani
Keyword(s):  
White Male ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Male Rats ◽  
Musa Paradisiaca ◽  
Activity Test ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Banana Leaf

Anti-inflammatory activity test of ethanolic extract of banana leaf (Musa Paradisiaca L.) on carrageenan-induced paw edema in white male rats (Rattus novergicus L.) has been conducted. It was aimed to investigate and to determine the anti-inflammatory activity and its effective dose. The extract was prepared by maceration method using ethanol 96%. Anti-inflammatory activity test was performed in five different groups. Each group consisted of 5 rats. The 1st group (negative control) was given 0.5% CMC-Na suspension; the 2nd group (positive control) was given diclofenac sodium 9 mg/KgBW; the 3rd, 4th, and 5th groups were successively given the banana leaf extract as much as 500, 750 and 1000 mg/KgBW. Each rat was then induced by 1% carrageenan and tested using subplantar method.  The inflamed paw diameter was measured using a calliper while the inflamed paw volume using pletysmometer. The measurements were done for 6 hours long with intervals of 60 minutes. The data was statistically analyzed using ANOVA (analysis of variance). The results showed that the negative control had a significant difference with the other treatment groups which did not show any anti-inflammatory effect. In conclusion, ethanolic extract of banana leaf has effective anti-inflammatory activity at a dose of 750 mg/KgBW

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