Use of Electrospun Phenylalanine/Poly-ε-Caprolactone Chiral Hybrid Scaffolds to Promote Endothelial Remodeling

The fabrication of tissue-engineered vascular grafts to replace damaged vessels is a promising therapy for cardiovascular diseases. Endothelial remodeling in the lumen of TEVGs is critical for successful revascularization. However, the construction of well-functioning TEVGs remains a fundamental challenge. Herein, chiral hybrid scaffolds were prepared by electrospinning using D/L-phenylalanine based gelators [D(L)PHEG] and poly-ε-caprolactone (PCL). The chirality of scaffolds significantly affected the endothelial remodeling progress of TEVGs. Compared with L-phenylalanine based gelators/poly-ε-caprolactone (L/PCL) and PCL, D-phenylalanine based gelators/poly-ε-caprolactone (D/PCL) scaffolds enhanced cell adhesion, and proliferation and upregulated the expression of fibronectin-1, and vinculin. These results suggests that chiral hybrid scaffolds can promote endothelial remodeling of TEVGs by upregulating adhesion-associated protein levels. This study offers an innovative strategy for endothelial remodeling of TEVGs by fabricating chiral hybrid scaffolds, and provides new insight for the treatment of cardiovascular diseases.

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Enhanced smooth muscle cell adhesion and proliferation on protein-modified polycaprolactone-based copolymers

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.31889 ◽

2009 ◽

Vol 88A (2) ◽

pp. 520-530 ◽

Cited By ~ 21

Author(s):

Hanna Bramfeldt ◽

Patrick Vermette

Keyword(s):

Smooth Muscle ◽

Cell Adhesion ◽

Smooth Muscle Cell ◽

Muscle Cell ◽

Cell Adhesion And Proliferation

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Assessment of Electrospun Pellethane-Based Scaffolds for Vascular Tissue Engineering

Materials ◽

10.3390/ma14133678 ◽

2021 ◽

Vol 14 (13) ◽

pp. 3678

Author(s):

Vera Chernonosova ◽

Alexandr Gostev ◽

Ivan Murashov ◽

Boris Chelobanov ◽

Andrey Karpenko ◽

...

Keyword(s):

Vascular Tissue ◽

Ex Vivo ◽

Vascular Grafts ◽

Wistar Rat ◽

Graft Occlusion ◽

Suitable Candidate ◽

Large Animals ◽

Cell Adhesion And Proliferation

We examined the physicochemical properties and the biocompatibility and hemocompatibility of electrospun 3D matrices produced using polyurethane Pellethane 2363-80A (Pel-80A) blends Pel-80A with gelatin or/and bivalirudin. Two layers of vascular grafts of 1.8 mm in diameter were manufactured and studied for hemocompatibility ex vivo and functioning in the infrarenal position of Wistar rat abdominal aorta in vivo (n = 18). Expanded polytetrafluoroethylene (ePTFE) vascular grafts of similar diameter were implanted as a control (n = 18). Scaffolds produced from Pel-80A with Gel showed high stiffness with a long proportional limit and limited influence of wetting on mechanical characteristics. The electrospun matrices with gelatin have moderate capacity to support cell adhesion and proliferation (~30–47%), whereas vascular grafts with bivalirudin in the inner layer have good hemocompatibility ex vivo. The introduction of bivalirudin into grafts inhibited platelet adhesion and does not lead to a change hemolysis and D-dimers concentration. Study in vivo indicates the advantages of Pel-80A grafts over ePTFE in terms of graft occlusion, calcification level, and blood velocity after 6 months of implantation. The thickness of neointima in Pel-80A–based grafts stabilizes after three months (41.84 ± 20.21 µm) and does not increase until six months, demonstrating potential for long-term functioning without stenosis and as a suitable candidate for subsequent preclinical studies in large animals.

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Silk-based hybrid microfibrous mats as guided bone regeneration membranes

Journal of Materials Chemistry B ◽

10.1039/d0tb02687e ◽

2021 ◽

Author(s):

Mi Wu ◽

Zhengyi Han ◽

Wen Liu ◽

Jinrong Yao ◽

Bingjiao Zhao ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Cell Adhesion ◽

Bone Regeneration ◽

Silk Fibroin ◽

Guided Bone Regeneration ◽

Regenerated Silk Fibroin ◽

Marrow Mesenchymal Stem Cells ◽

Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

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Effect of Surface Potential on NIH3T3 Cell Adhesion and Proliferation

The Journal of Physical Chemistry C ◽

10.1021/jp504662c ◽

2014 ◽

Vol 118 (26) ◽

pp. 14464-14470 ◽

Cited By ~ 38

Author(s):

Hsun-Yun Chang ◽

Chih-Chieh Huang ◽

Kang-Yi Lin ◽

Wei-Lun Kao ◽

Hua-Yang Liao ◽

...

Keyword(s):

Cell Adhesion ◽

Surface Potential ◽

Nih3t3 Cell ◽

Cell Adhesion And Proliferation ◽

Effect Of Surface

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Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation

Scientific Reports ◽

10.1038/s41598-017-07361-y ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 30

Author(s):

Alice C. Taylor ◽

Citlali Helenes González ◽

Benjamin S. Miller ◽

Robert J. Edgington ◽

Patrizia Ferretti ◽

...

Keyword(s):

Stem Cell ◽

Cell Adhesion ◽

Neural Stem Cell ◽

Human Neural Stem Cell ◽

Surface Functionalisation ◽

Cell Adhesion And Proliferation

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Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization

Journal of Functional Biomaterials ◽

10.3390/jfb9040062 ◽

2018 ◽

Vol 9 (4) ◽

pp. 62 ◽

Cited By ~ 8

Author(s):

Gianluca Turco ◽

Davide Porrelli ◽

Eleonora Marsich ◽

Federica Vecchies ◽

Teresa Lombardi ◽

...

Keyword(s):

Cell Adhesion ◽

Clinical Performance ◽

Maxillofacial Surgery ◽

Chemical Characterization ◽

Bone Substitutes ◽

Human Cell Line ◽

Structural Features ◽

Oral And Maxillofacial Surgery ◽

Cell Adhesion And Proliferation

Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedical applications in the field of orthopedic and maxillofacial surgery. In particular, phosphate-based bone substitution materials have exhibited a high biocompatibility due to their chemical similitude with natural hydroxyapatite. Besides the nature of the biomaterial, its porous and interconnected architecture is essential for a correct osseointegration. This performance could be predicted with an extensive characterization of the biomaterial in vitro. Methods: In this study, we compared the biological, chemical, and structural features of four different commercially available bone substitutes derived from an animal or a synthetic source. To this end, µ-CT and SEM were used to describe the biomaterials structure. Both FTIR and EDS analyses were carried out to provide a chemical characterization. The results obtained by these techniques were correlated with cell adhesion and proliferation of the osteosarcoma MG-63 human cell line cultured in vitro. Results: The findings reported in this paper indicate a significant influence of both the nature and the structure of the biomaterials in cell adhesion and proliferation, which ultimately could affect the clinical performance of the biomaterials. Conclusions: The four commercially available bone substitutes investigated in this work significantly differed in terms of structural features, which ultimately influenced in vitro cell proliferation and may so affect the clinical performance of the biomaterials.

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Adhesion and Proliferation of Osteoblast-Like Cells on Porous Polyetherimide Scaffolds

BioMed Research International ◽

10.1155/2018/1491028 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Yanbo Zhang ◽

Ruiyan Li ◽

Wenzheng Wu ◽

Yun’an Qing ◽

Xiongfeng Tang ◽

...

Keyword(s):

Tissue Engineering ◽

Contact Angle ◽

Cell Adhesion ◽

Phase Composition ◽

Cell Morphology ◽

Solvent Casting ◽

Water Contact ◽

Particulate Leaching ◽

Cell Adhesion And Proliferation ◽

Better Than

The purpose of this work was to investigate the porous polyetherimide scaffold (P-PEIs) as an alternative biopolymer for bone tissue engineering. The P-PEIs was fabricated via solvent casting and particulate leaching technique. The morphology, phase composition, roughness, hydrophilicity, and biocompatibility of P-PEIs were evaluated and compared with polyetherimide (PEI) and Ti6Al4V disks. P-PEIs showed a biomimetic porous structure with a modulus of 78.95 ± 2.30 MPa. The water contact angle of P-PEIs was 75.4 ± 3.39°, which suggested that P-PEIs had a wettability surface. Moreover, P-PEIs provides a feasible environment for cell adhesion and proliferation. The relative cell adhesion capability and the cell morphology on P-PEIs were better than PEI and Ti6Al4V samples. Furthermore, the MC3T3-E1 cells on P-PEIs showed faster proliferation rate than other groups. It was revealed that the P-PEIs could be a potential material for the application of bone regeneration.

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Electrospun membranes of PELCL/PCL-REDV loading with miRNA-126 for enhancement of vascular endothelial cell adhesion and proliferation

Materials Science and Engineering C ◽

10.1016/j.msec.2017.12.005 ◽

2018 ◽

Vol 85 ◽

pp. 37-46 ◽

Cited By ~ 19

Author(s):

Fang Zhou ◽

Meiling Wen ◽

Peiqiong Zhou ◽

Yunhui Zhao ◽

Xiaoling Jia ◽

...

Keyword(s):

Cell Adhesion ◽

Endothelial Cell ◽

Vascular Endothelial Cell ◽

Vascular Endothelial ◽

Electrospun Membranes ◽

Cell Adhesion And Proliferation ◽

Endothelial Cell Adhesion

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Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells

International Journal of Molecular Sciences ◽

10.3390/ijms20215383 ◽

2019 ◽

Vol 20 (21) ◽

pp. 5383 ◽

Cited By ~ 2

Author(s):

Li Zhang ◽

Feifei Wang ◽

Qing Zhang ◽

Qiuming Liang ◽

Shumei Wang ◽

...

Keyword(s):

Endothelial Cells ◽

Cell Adhesion ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Umbilical Vein ◽

Caspase 9 ◽

Protein Levels ◽

Tnf Α ◽

Umbilical Vein Endothelial Cells ◽

Cell Adhesion Molecule 1

Inflammation is a key mediator in the progression of atherosclerosis (AS). Benzoinum, a resin secreted from the bark of Styrax tonkinensis, has been widely used as a form of traditional Chinese medicine in clinical settings to enhance cardiovascular function, but the active components of the resin responsible for those pharmaceutical effects remain unclear. To better clarify these components, a new phenylpropane derivative termed stybenpropol A was isolated from benzoinum and characterized via comprehensive spectra a nalysis. We further assessed how this phenylpropane derivative affected treatment of human umbilical vein endothelial cells (HUVECs) with tumor necrosis factor-α (TNF-α). Our results revealed that stybenpropol A reduced soluble intercellular cell adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1 (sVCAM-1), interleukin-8 (IL-8), and interleukin-1β (IL-1β) expression by ELISA, inhibited apoptosis, and accelerated nitric oxide (NO) release in TNF-α-treated HUVECs. We further found that stybenpropol A decreased VCAM-1, ICAM-1, Bax, and caspase-9 protein levels, and increased the protein levels of Bcl-2, IKK-β, and IκB-α. This study identified a new, natural phenylpropane derivative of benzoinum, and is the first to reveal its cytoprotective effects in the context of TNF-α-treated HUVECs via regulation of the NF-κB and caspase-9 signaling pathways.

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