Aldose Reductase B1 in Pig Seminal Plasma: Identification, Localization in Reproductive Tissues, and Relationship With Quality and Sperm Preservation

Aldose reductase B1 (AKR1B1), a NADPH-dependent enzyme that belongs to the aldo-keto reductase protein superfamily, has been reported to be involved in both male and female reproductive physiology. The objectives of this study were: (1) to evaluate the concentration of SP-AKR1B1 in pig ejaculate fractions; (2) to describe the immunohistochemical localization of AKR1B1 alongside the boar genital tract; (3) to evaluate the relationship between SP-AKR1B1 and sperm quality/functionality parameters. Ejaculates from seven boars (one ejaculate per boar) were collected in separate portions [the first 10 mL of the sperm rich fraction (SRF-P1), the rest of the SRF (SRF-P2), and the post-SRF (PSRF)], and the concentration of SP-AKR1B1 was assessed using an enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry and immunoblotting targeting was conducted in the reproductive tissues of these boars. Additionally, the entire ejaculates of 14 boars (one ejaculate per boar) were collected and split into three separate aliquots for: (i) SP-AKR1B1 quantification; (ii) assessment of sperm concentration and morphology; and (iii) evaluation of sperm quality and functionality parameters upon ejaculate collection (0 h) and after 72 h of liquid storage at 17°C. Concentration of AKR1B1 in the SP of SRF-P1 (458.2 ± 116.33 ng/mL) was lower (P < 0.05) than that of SRF-P2 (1105.0 ± 229.80 ng/mL) and PSRF (1342.4 ± 260.18 ng/mL). Monomeric and dimeric AKR1B1 forms were expressed alongside the reproductive tissues, except in the bulbourethral glands. No relationship between SP-AKR1B1 and sperm quality/functionality parameters was observed either at 0 h or after 72 h of storage at 17°C. In conclusion, AKR1B1 is expressed in the reproductive organs of boars (except bulbourethral glands) and a higher concentration is found in the PSRF suggesting that seminal vesicles would be the main secretory source. However, this enzyme does not appear to be related to sperm quality/functionality or to the sperm ability to withstand liquid storage at 17°C.

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Development of sperm sexing and associated assisted reproductive technology for sex preselection of captive bottlenose dolphins (Tursiops truncatus)

Reproduction Fertility and Development ◽

10.1071/rd05108 ◽

2006 ◽

Vol 18 (3) ◽

pp. 319 ◽

Cited By ~ 63

Author(s):

J. K. O'Brien ◽

T. R. Robeck

Keyword(s):

Tursiops Truncatus ◽

Sperm Quality ◽

Bottlenose Dolphins ◽

Sperm Concentration ◽

Motility Index ◽

Liquid Storage ◽

Directional Freezing

Research was conducted to develop sperm sorting and novel sperm preservation methodologies for sex predetermination in the bottlenose dolphin (Tursiops truncatus) using artificial insemination. In Study 1, the effect of seminal plasma (SP), sperm concentration and freezing rate (FR) on in vitro sperm quality of liquid-stored, non-sorted spermatozoa was examined. There was no effect (P > 0.05) of prefreeze SP addition on post-thaw quality (progressive motility, kinetic rating, sperm motility index (SMI), viability and acrosome integrity). Post-thaw motility parameters and viability were higher (P < 0.05) for slow FR than fast FR samples. In Study 2 investigating the effects of liquid storage and sorting on sperm quality, motility and SMI after sorting and centrifugation were lower (P < 0.05) than those of the initial ejaculate. The sort rate for enrichment (91 ± 4% purity) of X- and Y-bearing spermatozoa was 3400 ± 850 spermatozoa sex−1 s−1. In Study 3, compared with a modified straw method, directional freezing resulted in enhanced in vitro quality of sorted and non-sorted spermatozoa derived from liquid-stored semen (P < 0.05). In Study 4, endoscopic insemination of three dolphins with sorted, frozen–thawed X-bearing spermatozoa resulted in one conception and the birth of a female calf. High-purity sorting of dolphin spermatozoa, derived from liquid-stored semen, can be achieved with minimal loss of in vitro sperm quality and samples are functional in vivo.

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RELATIONSHIP OF FEMALE AGE AND SPERM QUALITY WITH THE SUCCESSFUL OF IUI LITERATURE REVIEW

Indonesian Midwifery and Health Sciences Journal ◽

10.20473/imhsj.v5i3.2021.232-241 ◽

2021 ◽

Vol 5 (3) ◽

pp. 232

Author(s):

Dhea Hesty Purwaningrum ◽

Sri Ratna Dwiningsih ◽

Sulistiawati Sulistiawati

Keyword(s):

Literature Review ◽

Pregnancy Rate ◽

Sperm Quality ◽

Intrauterine Insemination ◽

Research Question ◽

Sperm Concentration ◽

Live Birth Rate ◽

Developed Countries ◽

Relationship Of ◽

The Relationship

AbstractBackground: Infertility in developing countries is higher at 30% compared to developed countries at 5-8%. The factors that influence infertility are maternal (women) age and sperm factors. Therapy in handling infertility in the form of Assisted Reproductive Technology (ART) with one of them is Intrauterine Insemination (IUI). The success rate of IUI is the percentage of IUI programs that give results as desired depending on the type of calculation used such as pregnancy rate or live birth rate. Method: This research is a literature review. The research question with PICO standard is "What is relationship between woman's age and sperm quality with the success of Intrauterine Insemination?". 70 literatures were obtained from Cochrane, Sciencedirect, PubMed, and google scholar databases. Literature screening was done by looking at the inclusion and exclusion criteria that have been set, so that 7 literature will be reviewed. Results: From all the literature on the relationship of woman's age with the pregnancy rate in the IUI process stated that there was a significant relationship between the age of the woman with the success of IUI. Most of literature (75%) on the relationship of sperm quality with pregnancy rates in the IUI process states that there is a relationship between sperm quality and the success of IUI. The percentage of pregnancies in the IUI program can reach more than 10% with a sperm concentration >10x106/ml. Conclusion: From a review of 7 literature, the age of women who are ideal for IUI is 20-35 years with a pregnancy of 20%. In a sperm concentration >10x106/ml and sperm morphology ≥ 5% can get >10% pregnancy rate, and a pregnancy rate of >8% on sperm motility ≥ 75%.

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Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Biology ◽

10.3390/biology9090300 ◽

2020 ◽

Vol 9 (9) ◽

pp. 300 ◽

Cited By ~ 1

Author(s):

Sara Sadeghi ◽

Raquel Del Gallego ◽

Balma García-Colomer ◽

Ernesto A. Gómez ◽

Jesús L. Yániz ◽

...

Keyword(s):

Dilution Rate ◽

Dna Fragmentation ◽

Sperm Motility ◽

Storage Temperature ◽

Sperm Quality ◽

Sperm Concentration ◽

Effect Of Temperature ◽

Sperm Dna Fragmentation ◽

Liquid Storage ◽

Duration Of Storage

The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 °C or 17 °C), durations (0, 24 and 48 h) and sperm concentrations (250 × 106 sperm/mL (1:2 dilution rate), 166.7 × 106 sperm/mL (1:3 dilution rate) or 50 × 106 sperm/mL (1:10 dilution rate)) on sperm motility and kinetics were studied. In the second experiment, the effect of temperature, sperm washing and concentration on sperm motility and DNA fragmentation was studied. Finally, the effect of sperm concentration and duration of storage at 5 °C on sperm motility, response to oxidative stress and MMP was examined. We found that refrigerated liquid storage of goat sperm impaired sperm quality, such as motility, MMP and response to oxidation, as storage time increased; however, sperm DNA fragmentation index was not significantly affected. Liquid storage at 5 °C preserved higher total motility than at 17 °C. Moreover, we observed that the reduction of sperm concentration below 500 × 106 sperm/mL did not seem to improve the quality of spermatozoa conserved in milk-based extender in the conditions tested.

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0029 Light Emitted from Media Devices at Night is Associated with Decline in Sperm Quality

SLEEP ◽

10.1093/sleep/zsaa056.028 ◽

2020 ◽

Vol 43 (Supplement_1) ◽

pp. A12-A12

Author(s):

A Green ◽

S Barak ◽

L Shine ◽

A Kahane ◽

Y Dagan ◽

...

Keyword(s):

Digital Media ◽

Sperm Quality ◽

Sperm Count ◽

Sperm Concentration ◽

Significant Negative Correlation ◽

Sleep Habits ◽

Light Emitting ◽

Digital Devices ◽

Progressive Motility ◽

The Relationship

Abstract Introduction The last several decades have been characterized by the widespread usage of digital devices, especially smartphones. At the same time, there have been reports of male fertility decline. The aim of this study was to assess the relationship between evening exposure to the light-emitting screens of digital media devices and sperm quality. Methods Semen samples were obtained from 116 men adults aged between 21 and 59 (35.2 ±7.2) undergoing fertility evaluation for the following sperm variables: volume (mL), pH, sperm concentration (n/mL), motility percentage (progressive% + non-progressive motility%) and total sperm count. Exposure to the screens of electronic devices and sleep habits were obtained by means of a questionnaire. Results Smartphone and tablet usage in the evening and after bedtime was negatively correlated (p<0.05) with sperm motility, sperm progressive motility, and sperm concentration, and positively correlated with the percentage of immotile sperm. In addition, sleep duration was positively correlated with sperm total and progressive motility and negatively correlated with semen pH (p<0.05). A significant negative correlation was observed between subjective sleepiness and total and progressive motility as well as total motile sperm number (p<0.05). Conclusion The results of this study revealed a link between evening and post-bedtime exposure to light-emitting digital media screens and sperm quality. To the best of our knowledge, this is the first study to report these types of correlations between sperm quality and exposure time to SWL emitted from digital media, especially smartphones and tablets, in the evening and after bedtime. Support No Support

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Metabolite Profiling of Pig Seminal Plasma Identifies Potential Biomarkers for Sperm Resilience to Liquid Preservation

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.669974 ◽

2021 ◽

Vol 9 ◽

Author(s):

Yentel Mateo-Otero ◽

Pol Fernández-López ◽

Jordi Ribas-Maynou ◽

Jordi Roca ◽

Jordi Miró ◽

...

Keyword(s):

Seminal Plasma ◽

Sperm Quality ◽

Sperm Concentration ◽

Computer Assisted ◽

Sperm Analysis ◽

Liquid Storage ◽

Dependent Behavior ◽

Downstream Gene Expression ◽

Cell Signaling Pathways ◽

C Nmr

Metabolomic approaches allow the study of downstream gene expression events since metabolites are considered as the products of cell signaling pathways. For this reason, many studies in humans have already been conducted to determine the influence of the metabolites present in seminal plasma (SP) on sperm physiology, and to identify putative biomarkers. However, in livestock species, these relationships are yet to be uncovered. Thus, the present study aimed to explore: (i) if concentrations of metabolites in pig SP are related to sperm quality and functionality, and (ii) if they could predict the sperm resilience to liquid storage at 17°C. To this end, 28 ejaculates were individually collected and split into three aliquots: one was used for SP analysis through nuclear magnetic resonance (NMR) spectroscopy; another served for the evaluation of sperm concentration and morphology; and the last one was utilized to determine sperm functionality parameters using computer-assisted sperm analysis (CASA) and flow cytometry after 0 h and 72 h of liquid-storage at 17°C. NMR analysis allowed the identification and quantification of 23 metabolites present in pig SP which, except for fumarate, were not observed to follow a breed-dependent behavior. Moreover, specific relationships between metabolites and sperm variables were identified: (i) glutamate, methanol, trimethylamine N-oxide, carnitine, and isoleucine were seen to be related to some sperm quality and functionality parameters evaluated immediately after semen collection; (ii) leucine, hypotaurine, carnitine and isoleucine were found to be associated to the sperm ability to withstand liquid storage; and (iii) Bayesian multiple regression models allowed the identification of metabolite patterns for specific sperm parameters at both 0 h and 72 h. The identification of these relationships opens up the possibility of further investigating these metabolites as potential sperm functional biomarkers.

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Seasonal Variation of Sperm Quality and the Relationship between Spermatocrit and Sperm Concentration in YamúBrycon amazonicus

North American Journal of Aquaculture ◽

10.1577/a06-002.1 ◽

2007 ◽

Vol 69 (2) ◽

pp. 159-165 ◽

Cited By ~ 6

Author(s):

Pablo E. Cruz-Casallas ◽

Víctor M. Medina-Robles ◽

Yohana M. Velasco-Santamaría

Keyword(s):

Seasonal Variation ◽

Sperm Quality ◽

Sperm Concentration ◽

The Relationship

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Seminal Plasma Anti-Müllerian Hormone: A Potential AI-Boar Fertility Biomarker?

Biology ◽

10.3390/biology9040078 ◽

2020 ◽

Vol 9 (4) ◽

pp. 78 ◽

Cited By ~ 1

Author(s):

Isabel Barranco ◽

Beatriz Fernandez-Fuertes ◽

Lorena Padilla ◽

Ariadna Delgado-Bermúdez ◽

Asta Tvarijonaviciute ◽

...

Keyword(s):

Seminal Plasma ◽

Sperm Quality ◽

Sperm Concentration ◽

Predictive Biomarker ◽

Membrane Lipid ◽

Enzyme Linked Immunosorbent Assay ◽

Lipid Disorder ◽

Size Number ◽

Farrowing Rate

The anti-Müllerian hormone (AMH), a Sertoli cell-secreted glycoprotein that is present in seminal plasma (SP), is considered as a marker of spermatogenesis in humans. This study aimed to evaluate the presence of this hormone in boar SP, together with its putative relationship with sperm quality, function, and in vivo fertility parameters in liquid-stored semen samples. The concentration of SP-AMH was assessed in 126 ejaculates from artificial insemination (AI)-boars (n = 92) while using a commercial Enzyme-Linked ImmunoSorbent Assay (ELISA) kit with monoclonal antibodies specific for Sus scrofa AMH (CEA228Po, Cloud-clone). Sperm quality (concentration, motility, viability, and acrosome damage) and functionality (membrane lipid disorder and intracellular H2O2 generation) were assessed in semen samples at 0 and 72 h of liquid-storage. In addition, fertility parameters from 3113 sows inseminated with the AI-boars were recorded in terms of farrowing rate, litter size, number of stillbirths per litter, and the duration of pregnancy over a 12-month period. The results revealed that the SP-AMH concentration varied widely among boar ejaculates, with no differences among breeds. Moreover, the SP-AMH concentration proved to be a good predictive biomarker for sperm concentration (p ˂ 0.05), but poor for other sperm quality, functionality, and in vivo fertility parameters of liquid-stored semen samples from AI-boars.

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MiR-125a-5p Alleviates Dysfunction and Inflammation of Pentylenetetrazol- induced Epilepsy Through Targeting Calmodulin-dependent Protein Kinase IV (CAMK4)

Current Neurovascular Research ◽

10.2174/1567202616666190906125444 ◽

2019 ◽

Vol 16 (4) ◽

pp. 365-372 ◽

Cited By ~ 4

Author(s):

Qishuai Liu ◽

Li Wang ◽

Guizhen Yan ◽

Weifa Zhang ◽

Zhigang Huan ◽

...

Keyword(s):

Protein Kinase ◽

Target Gene ◽

Luciferase Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Inflammatory Factor ◽

Dependent Protein Kinase ◽

Expression Levels ◽

Dependent Protein ◽

Polymerase Chain ◽

The Relationship

Background: MicroRNAs (miRNA) are known to play a key role in the etiology and treatment of epilepsy through controlling the expression of gene. However, miR-125a-5p in the epilepsy is little known. Epilepsy in rat models was induced by Pentylenetetrazol (PTZ) and miR- 125a-5p profiles in the hippocampus were investigated in our experiment. Also, the relationship between miR-125a-5p and calmodulin-dependent protein kinase IV (CAMK4) was identified and the related mechanism was also illustrated. Methods: The miR-125a-5p mRNA expression levels were evaluated by quantitative real time polymerase chain reaction (qRT-PCR). Western Blot (WB) was used to analyze the CAMK4 protein expression levels. Seizure score, latency and duration were determined based on a Racine scale. The enzyme-linked immunosorbent assay (ELISA) was used to analyze the inflammatory factor expression. The relationship between miR-125a-5p and CAMK4 was detected through dual luciferase assay. Results: Downregulation of miR-125a-5p was observed in the hippocampus of PTZ-induced epilepsy rats. The overexpression of miR-125a-5p attenuated seizure and decreased inflammatory factor level in the hippocampus of PTZ-induced rats. The miR-125a-5p alleviated epileptic seizure and inflammation in PTZ-induced rats by suppressing its target gene, CAMK4. Conclusion: miR-125a-5p may represent a novel therapeutic treatment for PTZ-induced epilepsy by preventing the activation of CAMK4.

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CagA-PositiveHelicobacter pyloriInfection and Reduced Sperm Motility, Vitality, and Normal Morphology

Disease Markers ◽

10.1155/2013/919174 ◽

2013 ◽

Vol 35 ◽

pp. 229-234 ◽

Cited By ~ 8

Author(s):

E. Moretti ◽

G. Collodel ◽

L. Mazzi ◽

M. S. Campagna ◽

N. Figura

Keyword(s):

Sperm Motility ◽

Normal Forms ◽

Molecular Mimicry ◽

Sperm Quality ◽

Semen Analysis ◽

Normal Morphology ◽

Partial Linear ◽

Caga Status ◽

The Relationship ◽

Autoimmune Phenomena

Helicobacter pylori(HP) infection, particularly when caused by strains expressing CagA, may be considered a concomitant cause of male and female reduced fertility. This study explored, in 87 HP-infected males, the relationship between infection by CagA-positive HP strains and sperm parameters. HP infection and CagA status were determined by ELISA and Western blotting; semen analysis was performed following WHO guidelines. The amino acid sequence of human enzymes involved in glycolysis and oxidative metabolism were “blasted” with peptides expressed by HP J99. Thirty-seven patients (42.5%) were seropositive for CagA. Sperm motility (18% versus 32%; ), sperm vitality (35% versus 48%; ) and the percentage of sperm with normal forms (18% versus 22%; ) in the CagA-positive group were significantly reduced versus those in the CagA-negative group. All the considered enzymes showed partial linear homology with HP peptides, but four enzymes aligned with four different segments of the samecagisland protein. We hypothesize a relationship between infection by strains expressing CagA and decreased sperm quality. Potentially increased systemic levels of inflammatory cytokines that occur in infection by CagA-positive strains and autoimmune phenomena that involve molecular mimicry could explain the pathogenetic mechanism of alterations observed.

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Plasma angiopoietin-2 is associated with age-related deficits in cognitive sub-scales in Ugandan children following severe malaria

Malaria Journal ◽

10.1186/s12936-020-03545-6 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Benson J. Ouma ◽

Paul Bangirana ◽

John M. Ssenkusu ◽

Dibyadyuti Datta ◽

Robert O. Opoka ◽

...

Keyword(s):

Severe Malaria ◽

Parental Education ◽

Preschool Education ◽

Kidney Injury ◽

Malaria Episode ◽

Enzyme Linked Immunosorbent Assay ◽

Testing Time ◽

Age Related ◽

Angiopoietin 2 ◽

The Relationship

Abstract Background Elevated angiopoietin-2 (Angpt-2) concentrations are associated with worse overall neurocognitive function in severe malaria survivors, but the specific domains affected have not been elucidated. Methods Ugandan children with severe malaria underwent neurocognitive evaluation a week after hospital discharge and at 6, 12 and 24 months follow-up. The relationship between Angpt-2 concentrations and age-adjusted, cognitive sub-scale z-scores over time were evaluated using linear mixed effects models, adjusting for disease severity (coma, acute kidney injury, number of seizures in hospital) and sociodemographic factors (age, gender, height-for-age z-score, socio-economic status, enrichment in the home environment, parental education, and any preschool education of the child). The Mullen Scales of Early Learning was used in children < 5 years and the Kaufman Assessment Battery for Children 2nd edition was used in children ≥ 5 years of age. Angpt-2 levels were measured on admission plasma samples by enzyme-linked immunosorbent assay. Adjustment for multiple comparisons was conducted using the Benjamini–Hochberg Procedure of False Discovery Rate. Results Increased admission Angpt-2 concentration was associated with worse outcomes in all domains (fine and gross motor, visual reception, receptive and expressive language) in children < 5 years of age at the time of severe malaria episode, and worse simultaneous processing and learning in children < 5 years of age at the time of severe malaria who were tested when ≥ 5 years of age. No association was seen between Angpt-2 levels and cognitive outcomes in children ≥ 5 years at the time of severe malaria episode, but numbers of children and testing time points were lower for children ≥ 5 years at the time of severe malaria episode. Conclusion Elevated Angpt-2 concentration in children with severe malaria is associated with worse outcomes in multiple neurocognitive domains. The relationship between Angpt-2 and worse cognition is evident in children < 5 years of age at the time of severe malaria presentation and in selected domains in older years.

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