miR-6807-5p Inhibited the Odontogenic Differentiation of Human Dental Pulp Stem Cells Through Directly Targeting METTL7A

Background: Tooth tissue regeneration mediated by mesenchymal stem cells (MSCs) has become the most ideal treatment. Although the known regulatory mechanism and some achievements have been discovered, directional differentiation cannot effectively induce regeneration of tooth tissue. In this study, we intended to explore the function and mechanism of miR-6807-5p and its target gene METTL7A in odontogenic differentiation.Methods: In this study, human dental pulp stem cells (DPSCs) were used. Alkaline phosphatase (ALP), Alizarin red staining (ARS), and calcium ion quantification were used to detect the odontogenic differentiation of miR-6807-5p and METTL7A. Real-time RT-PCR, western blot, dual-luciferase reporter assay, and pull-down assay with biotinylated miRNA were used to confirm that METTL7A was the downstream gene of miR-6807-5p. Protein mass spectrometry and co-immunoprecipitation (Co-IP) were used to detect that SNRNP200 was the co-binding protein of METTL7A.Results: After mineralized induction, the odontogenic differentiation was enhanced in the miR-6807-5p-knockdown group and weakened in the miR-6807-5p-overexpressed group compared with the control group. METTL7A was the downstream target of miR-6807-5p. After mineralized induction, the odontogenic differentiation was weakened in the METTL7A-knockdown group and enhanced in the METTL7A-overexpressed group compared with the control group. SNRNP200 was the co-binding protein of METTL7A. The knockdown of SNRNP200 inhibited the odontogenic differentiation of DPSCs.Conclusion: This study verified that miR-6807-5p inhibited the odontogenic differentiation of DPSCs. The binding site of miR-6807-5p was the 3′UTR region of METTL7A, which was silenced by miR-6807-5p. METTL7A promoted the odontogenic differentiation of DPSCs. SNRNP200, a co-binding protein of METTL7A, promoted the odontogenic differentiation of DPSCs.

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The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways

Stem Cells International ◽

10.1155/2019/4793518 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 9

Author(s):

Jintao Wu ◽

Na Li ◽

Yuan Fan ◽

Yanqiu Wang ◽

Yongchun Gu ◽

...

Keyword(s):

Stem Cells ◽

Signaling Pathways ◽

Conditioned Medium ◽

Dental Pulp ◽

Mapk Signaling ◽

Dental Pulp Stem Cells ◽

Control Group ◽

Odontogenic Differentiation ◽

Human Dental Pulp ◽

Mapk Signaling Pathways

The calcined tooth powder (CTP), a type of allogeneic biomimetic mineralized material, has been confirmed that can promote new bone formation when obtained at high temperature. The aim of this study was to investigate effects of the conditioned medium of calcined tooth powder (CTP-CM) on the osteogenic and odontogenic differentiation of human dental pulp stem cells (hDPSCs) and the underlying mechanisms involved. First, ALP activity assay determined that 200 μg/mL was the optimal concentration of CTP-CM for the following experiments. CTP-CM had no significant effect on the proliferation of hDPSCs as indicated by CCK-8 and FCM analysis. Both the gene and protein (DSPP/DSPP, RUNX2/RUNX2, OCN/OCN, OSX/OSX, OPN/OPN, ALP/ALP, and COL-1/COL-1) expression levels increased in the CTP-CM-induced hDPSC group as compared with those in the control group at day 3 or 7, showing the positive regulation of CTP-CM on the osteo/odontogenic differentiation of hDPSCs. Mechanistically, MAPK signaling pathways were activated after the CTP-CM treatment, and the inhibitors targeting MAPK were identified which weakened the effects of CTM-CM on the committed differentiation of hDPSCs. These findings could lead to the creation of stem cell therapies for dental regeneration.

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miR-217 Suppresses Osteogenic Differentiation of Human Dental Pulp Stem Cells by Down-Regulating Sirtuin 1

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2020.2373 ◽

2020 ◽

Vol 10 (7) ◽

pp. 978-986

Author(s):

Haiquan Yue ◽

Yidan Guo ◽

Juan Song ◽

Ruimin Liu

Keyword(s):

Stem Cells ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Sirtuin 1 ◽

Luciferase Reporter ◽

Induction Medium ◽

Alizarin Red ◽

Human Dental Pulp ◽

Osteogenic Induction

The paper is committed to uncovering the effect of miR-217 on osteogenic differentiation of human dental pulp stem cells (hDPSCs) and its mechanism. hDPSCs were separated from human dental pulp tissues for measurement of stemness. The osteogenic differentiation of hDPSCs was induced in an osteogenic induction medium. The hDPSCs were transfected with miR-217 mimic, miR-217 inhibitor and/or sh-SIRT1 accordingly. The expressions of miR-217 and SIRT1 were detected in hDPSCs after cell transfection and osteogenic differentiation. Calcium nodules were showed by alizarin red staining. Moreover, the expressions of osteogenic differentiation-related genes were also assessed. The binding of miR-217 to SIRT1 was predicted on starBase and further determined by dual-luciferase reporter assay. Down-regulated miR-217 and up-regulated SIRT1 were found during osteogenic differentiation of hDPSCs. The osteogenic differentiation of hDPSCs was suppressed after transfection of miR-217 mimic or sh-SIRT1 while promoted by miR-217 inhibition. Taken together, miR-217 can suppress osteogenic differentiation of hDPSCs by negatively regulating SIRT1.

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Efficacy of Photobiomodulation and Vitamin D on Odontogenic Activity of Human Dental Pulp Stem Cells

Journal of lasers in medical sciences ◽

10.34172/jlms.2021.30 ◽

2021 ◽

Vol 12 (1) ◽

pp. e30-e30

Author(s):

Latifa M. Abdelgawad ◽

Nehal Salah ◽

Dina Sabry ◽

Marwa Abdelgwad

Keyword(s):

Stem Cells ◽

Vitamin D ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Control Group ◽

Mrna Levels ◽

Pulp Tissue ◽

Odontogenic Differentiation ◽

Group I ◽

Human Dental Pulp

Introduction: The regeneration of dental pulp tissue using human dental pulp stem cells (HDPSCs) has attracted increasing attention in recent years. Recent studies have suggested that several factors such as photobiomodulation (PBM) and vitamin D affect the proliferation and differentiation of HDPSCs. Therefore, the present study evaluated the effects of PBM and vitamin D on odontogenic differentiation of HDPSCs for dentin -like tissue formation. Methods: HDPSCs were collected, isolated, and characterized and then divided into six groups: group I, control; group II, vitamin D (10-7 Mol); group III, irradiation at 1 J/cm2 of 810 nm diode laser; group IV, irradiation at 1 J/cm2 and culture with vitamin D; group V, irradiation at 2 J/cm2 , and group VI, irradiation at 2 J/cm2 and culture with vitamin D, cell viability assay was measured through MTT. Alkaline phosphatase (ALP) enzyme activity and mRNA levels of vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), and dentin sialophosphoprotein (DSPP) were also assessed. Results: PBM at 1 and 2 J/cm2 combined with vitamin D significantly promoted HDPSCs proliferation through MTT assay and odontogenic differentiation through gene expression of VEGF, BMP-2, and DSPP levels (P<0.0001). Conclusion: PBM at 2 J/cm2 combined with vitamin D enhanced the HDPSCs proliferation and odontogenic differentiation and thus could be a novel strategy for dentin regeneration in dentistry.

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Nano Hydroxyapatite & Mineral Trioxide Aggregate Efficiently Promote Odontogenic Differentiation of Dental Pulp Stem Cells

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2018.368 ◽

2018 ◽

Vol 6 (9) ◽

pp. 1727-1731 ◽

Cited By ~ 2

Author(s):

Ahmed Khaled Hanafy ◽

Souzy F. Shinaishin ◽

Ghada Nour Eldeen ◽

Riham Mohammed Aly

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Mineral Trioxide Aggregate ◽

Dental Pulp Stem Cells ◽

Control Group ◽

Promoting Effect ◽

Hydroxy Apatite ◽

Alizarin Red ◽

Odontogenic Differentiation ◽

And Function

BACKGROUND: There has been an urge to shift from conventional therapies to the more promising regenerative strategy since conventional treatment relies on synthetic materials to fill defects and replace missing tissues, lacking the ability to restore the tissues’ physiological architecture and function. AIM: The present study focused on the assessment of the role of two commonly used biomaterials namely; mineral trioxide aggregate (MTA) and nano hydroxy-apatite as promoters of odontogenic differentiation of dental pulp stem cells (DPSCs). METHODS: DPSCs were isolated, cultured in odontogenic media and divided into three groups; control group, MTA group and nanohydroxyapatite group. Odontogenic differentiation was assessed by tracing genes characteristic of different stages of odontoblasts via qRT-PCR. Calcific nodules formation was evaluated by Alizarin red staining. RESULTS: Results demonstrated that both MTA and nanohydroxyapatite were capable of enhancing odontogenic differentiation of DPSCs. CONCLUSION: Nano hydroxyapatite was found to have a higher promoting effect. However, in the absence of an odontogenic medium, MTA and nanohydroxyapatite could not enhance the odontogenic differentiation of DPSCs.

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Evaluation of Resin-Based Material Containing Copaiba Oleoresin (Copaifera Reticulata Ducke): Biological Effects on the Human Dental Pulp Stem Cells

Biomolecules ◽

10.3390/biom10070972 ◽

2020 ◽

Vol 10 (7) ◽

pp. 972

Author(s):

Roberta Souza D’Almeida Couto ◽

Maria Fernanda Setubal Destro Rodrigues ◽

Leila Soares Ferreira ◽

Ivana Márcia Alves Diniz ◽

Fernando de Sá Silva ◽

...

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Biological Effects ◽

Dental Pulp Stem Cells ◽

Nodule Formation ◽

Alizarin Red ◽

Pulp Capping ◽

Hsp 27 ◽

Dental Pulp Capping ◽

Human Dental Pulp

The purpose of this study was to analyze in vitro the biological effects on human dental pulp stem cells triggered in response to substances leached or dissolved from two experimental cements for dental pulp capping. The experimental materials, based on extracts from Copaifera reticulata Ducke (COP), were compared to calcium hydroxide [Ca(OH)2] and mineral trioxide aggregate (MTA), materials commonly used for direct dental pulp capping in restorative dentistry. For this, human dental pulp stem cells were exposed to COP associated or not with Ca(OH)2 or MTA. Cell cytocompatibility, migration, and differentiation (mineralized nodule formation (Alizarin red assay) and gene expression (RT-qPCR) of OCN, DSPP, and HSP-27 (genes regulated in biomineralization events)) were evaluated. The results showed that the association of COP reduced the cytotoxicity of Ca(OH)2. Upregulations of the OCN, DSPP, and HSP-27 genes were observed in response to the association of COP to MTA, and the DSPP and HSP-27 genes were upregulated in the Ca(OH)2 + COP group. In up to 24 h, cell migration was significantly enhanced in the MTA + COP and Ca(OH)2 + COP groups. In conclusion, the combination of COP with the currently used materials for dental pulp capping [Ca(OH)2 and MTA] improved the cell activities related to pulp repair (i.e., cytocompatibility, differentiation, mineralization, and migration) including a protective effect against the cytotoxicity of Ca(OH)2.

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The role of osteomodulin on osteo/odontogenic differentiation in human dental pulp stem cells

BMC Oral Health ◽

10.1186/s12903-018-0680-6 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 5

Author(s):

Wenzhen Lin ◽

Li Gao ◽

Wenxin Jiang ◽

Chenguang Niu ◽

Keyong Yuan ◽

...

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Odontogenic Differentiation ◽

Human Dental Pulp

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WITHDRAWN: Overexpression of GLI2 induces odontogenic differentiation in human dental pulp stem cells through activation of the Wnt/β-catenin signaling pathway

Life Sciences ◽

10.1016/j.lfs.2019.117178 ◽

2019 ◽

pp. 117178

Author(s):

Haiying Zheng ◽

Chao Tian ◽

Xin Zeng ◽

Tao Liu

Keyword(s):

Stem Cells ◽

Signaling Pathway ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Odontogenic Differentiation ◽

Human Dental Pulp

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Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

Molecular Medicine Reports ◽

10.3892/mmr.2014.2481 ◽

2014 ◽

Vol 10 (4) ◽

pp. 1899-1906 ◽

Cited By ~ 13

Author(s):

WEN ZHANG ◽

WEI LIU ◽

JUNQI LING ◽

ZHENGMEI LIN ◽

YAN GAO ◽

...

Keyword(s):

Stem Cells ◽

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Vascular Endothelial ◽

Odontogenic Differentiation ◽

Human Dental Pulp ◽

Endothelial Growth Factor

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Pentraxin-3 Modulates Osteogenic/Odontogenic Differentiation and Migration of Human Dental Pulp Stem Cells

International Journal of Molecular Sciences ◽

10.3390/ijms20225778 ◽

2019 ◽

Vol 20 (22) ◽

pp. 5778

Author(s):

Yeon Kim ◽

Joo-Yeon Park ◽

Hyun-Joo Park ◽

Mi-Kyoung Kim ◽

Yong-Il Kim ◽

...

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Tissue Repair ◽

Therapeutic Potential ◽

Dental Pulp Stem Cells ◽

Pentraxin 3 ◽

Odontogenic Differentiation ◽

Human Dental Pulp ◽

And Migration

Pentraxin-3 (PTX3) is recognized as a modulator of inflammation and a mediator of tissue repair. In this study, we characterized the role of PTX3 on some biological functions of human dental pulp stem cells (HDPSCs). The expression level of PTX3 significantly increased during osteogenic/odontogenic differentiation of HDPSCs, whereas the knockdown of PTX3 decreased this differentiation. Silencing of PTX3 in HDPSCs inhibited their migration and C-X-C chemokine receptor type 4 (CXCR4) expression. Our present study indicates that PTX3 is involved in osteogenic/odontogenic differentiation and migration of HDPSCs, and may contribute to the therapeutic potential of HDPSCs for regeneration and repair.

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